FastQCFastQC Report
Thu 26 May 2016
SRR1364541_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364541_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3277009
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73620.22465608120087557No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62970.19215693334989314No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37010.11293835323613696No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA65850.057.5882761
GTATCAA118400.050.359461
ATCAACG152150.039.1901253
TCAACGC153350.038.8446124
CAACGCA155000.038.5005235
AACGCAG157500.038.002736
TATCAAC159300.037.169372
GTACATG171300.034.1115531
TACATGG174950.032.4824642
ACGCAGA189200.031.4782317
ACATGGG174200.031.3912853
CGCAGAG190950.031.033958
GCAGAGT207100.027.5509079
GAGTACT107700.026.13020312-13
CATGGGA110800.026.1282924
CATGGGG72050.025.7123014
AGTACTT112400.024.8787712-13
AGAGTAC190700.024.25663210-11
GTACTTT117900.023.66771914-15
CAGAGTA200200.023.34333610-11
Produced by FastQC (version 0.11.5)