##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364541_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3277009 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.289682146127763 31.0 30.0 34.0 26.0 34.0 2 30.21746202100757 31.0 30.0 34.0 26.0 34.0 3 30.49356898317948 31.0 30.0 34.0 26.0 34.0 4 34.04359798828749 37.0 35.0 37.0 30.0 37.0 5 33.49989334786691 35.0 35.0 37.0 28.0 37.0 6 33.52030006631047 37.0 35.0 37.0 28.0 37.0 7 33.32043549468433 37.0 35.0 37.0 27.0 37.0 8 33.25507009593199 37.0 35.0 37.0 26.0 37.0 9 34.5359503132277 38.0 35.0 39.0 27.0 39.0 10-11 34.475883343622186 38.0 34.5 39.0 27.0 39.0 12-13 34.368864107483375 38.0 34.0 39.0 26.0 39.0 14-15 35.36539142858625 39.0 34.0 41.0 25.5 41.0 16-17 35.36651165742907 39.0 34.0 41.0 26.0 41.0 18-19 35.392325135512294 39.0 34.0 41.0 26.0 41.0 20-21 35.40784950544841 39.0 34.0 41.0 26.0 41.0 22-23 35.36492148785676 39.0 34.0 41.0 26.0 41.0 24-25 35.32626764223107 39.0 34.0 41.0 25.5 41.0 26-27 35.20803162273891 39.0 34.0 41.0 25.0 41.0 28-29 35.064562379901915 39.0 34.0 41.0 25.0 41.0 30-31 34.92184855152976 39.0 34.0 41.0 24.5 41.0 32-33 34.738636817903156 39.0 34.0 41.0 24.0 41.0 34-35 34.568432219746725 38.0 34.0 41.0 24.0 41.0 36-37 34.38147331911508 38.0 33.0 41.0 23.0 41.0 38-39 34.22216920978856 38.0 33.0 40.0 22.0 41.0 40-41 34.25764775134887 38.0 33.0 41.0 22.0 41.0 42-43 34.133418919508614 38.0 33.0 41.0 20.0 41.0 44-45 33.97492042286122 38.0 33.0 41.0 18.5 41.0 46-47 33.74334446441862 38.0 33.0 41.0 15.5 41.0 48-49 33.47189342476631 38.0 33.0 40.5 13.5 41.0 50-51 33.30386871076644 38.0 32.5 40.0 9.5 41.0 52-53 33.125579758859374 38.0 32.0 40.0 8.5 41.0 54-55 32.93646965876505 38.0 32.0 40.0 8.0 41.0 56-57 32.72910465000248 38.0 32.0 40.0 7.0 41.0 58-59 32.52664960639412 37.0 31.0 40.0 6.0 41.0 60-61 32.29407609194848 37.0 31.0 40.0 2.0 41.0 62-63 32.1180401091361 36.5 31.0 40.0 2.0 41.0 64-65 31.982698094512408 36.0 31.0 40.0 2.0 41.0 66-67 31.682052597353255 36.0 31.0 39.0 2.0 41.0 68-69 31.39221726275393 35.0 31.0 39.0 2.0 41.0 70-71 31.07819478066737 35.0 30.0 39.0 2.0 40.5 72-73 30.782817654757736 35.0 30.0 37.5 2.0 40.0 74-75 30.425352508949473 35.0 30.0 37.0 2.0 39.5 76-77 30.107191496880233 35.0 30.0 36.5 2.0 39.0 78-79 29.815187568908108 35.0 30.0 36.0 2.0 39.0 80-81 29.527731995853536 35.0 29.0 36.0 2.0 37.5 82-83 29.23611882054642 34.0 29.0 35.0 2.0 37.0 84-85 29.005421712299235 34.0 29.0 35.0 2.0 37.0 86-87 28.793900932222037 34.0 29.0 35.0 2.0 36.0 88-89 28.61729018748499 34.0 29.0 35.0 2.0 36.0 90-91 28.475894939562266 34.0 29.0 35.0 2.0 36.0 92-93 28.369844269576312 34.0 29.0 35.0 2.0 35.0 94-95 28.258311161183872 34.0 29.0 35.0 2.0 35.0 96-97 28.146356326760163 34.0 29.0 35.0 2.0 35.0 98-99 28.069419858169447 34.0 29.0 35.0 2.0 35.0 100-101 27.975190791358827 34.0 29.0 35.0 2.0 35.0 102-103 26.693514726386166 32.0 25.5 34.0 2.0 35.0 104-105 27.644716111551723 34.0 27.0 35.0 2.0 35.0 106-107 27.806859700415835 34.0 28.0 35.0 2.0 35.0 108-109 27.81665826978199 34.0 28.0 35.0 2.0 35.0 110-111 27.744953248526322 34.0 28.0 35.0 2.0 35.0 112-113 27.663598116453144 34.0 27.5 35.0 2.0 35.0 114-115 27.5670649668646 34.0 27.0 35.0 2.0 35.0 116-117 27.448875331132747 34.0 27.0 35.0 2.0 35.0 118-119 27.31582351467451 34.0 27.0 35.0 2.0 35.0 120-121 27.12478574212033 34.0 27.0 35.0 2.0 35.0 122-123 26.839837791107684 34.0 27.0 35.0 2.0 35.0 124-125 24.991128190371157 31.5 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6004778624225438 1101 2 0.7797781543831981 1101 3 0.597060841133839 1101 4 0.5669179090019227 1101 5 0.6305948728447248 1101 6 0.5795391723297811 1101 7 0.6684630244960559 1101 8 0.7934661882763479 1101 9 1.0070031245243385 1101 10-11 0.9361022989938377 1101 12-13 0.8123130569134105 1101 14-15 0.9591158794390182 1101 16-17 0.9075152444979366 1101 18-19 1.105419324930537 1101 20-21 1.13133350594984 1101 22-23 1.0118588456423154 1101 24-25 1.1111161155981932 1101 26-27 1.0977276992230287 1101 28-29 1.1423360501645732 1101 30-31 1.1447279288512675 1101 32-33 1.1240630517595065 1101 34-35 1.2201258838020053 1101 36-37 1.2472422697693375 1101 38-39 1.1387738559471359 1101 40-41 0.997282583881784 1101 42-43 0.993777477116808 1101 44-45 0.9549785338304062 1101 46-47 1.0537662210351328 1101 48-49 1.1177267817984458 1101 50-51 1.1130611815936646 1101 52-53 1.0923367371016397 1101 54-55 1.0991489284632578 1101 56-57 1.0422757952051214 1101 58-59 1.0406520856976158 1101 60-61 0.9173940827681761 1101 62-63 0.9370155693467268 1101 64-65 0.860191023752062 1101 66-67 1.0518376035842252 1101 68-69 1.0968338043504957 1101 70-71 1.0451603158227698 1101 72-73 0.9753250059733602 1101 74-75 0.9951954774318637 1101 76-77 1.0151495831673003 1101 78-79 0.9957519121360541 1101 80-81 0.8408796852300959 1101 82-83 1.058644605164627 1101 84-85 1.0568552444102366 1101 86-87 1.0854172248123533 1101 88-89 1.0168482894157407 1101 90-91 1.0051013551951726 1101 92-93 1.013542563354779 1101 94-95 0.995243428607484 1101 96-97 1.0021334243876332 1101 98-99 1.0156936330326083 1101 100-101 1.0543493562404862 1101 102-103 0.9402445028173432 1101 104-105 1.0031089204968602 1101 106-107 0.9318905298175011 1101 108-109 0.9881161325331362 1101 110-111 0.9202985910779446 1101 112-113 0.9847876521891159 1101 114-115 1.0205656814188373 1101 116-117 0.9298612060534914 1101 118-119 0.9138320678835896 1101 120-121 0.9098605975435312 1101 122-123 0.9117195203126833 1101 124-125 1.1400839008478023 1102 1 0.22201762876480302 1102 2 -0.5182764647037992 1102 3 0.08696839578473003 1102 4 0.15493733100646523 1102 5 0.36603515442138246 1102 6 0.2840367657822753 1102 7 0.150633581924545 1102 8 0.30777019777531933 1102 9 0.4858732133651671 1102 10-11 0.5865697204895 1102 12-13 0.6439228993746653 1102 14-15 0.7719672671794058 1102 16-17 0.7242923791161715 1102 18-19 0.760717545993657 1102 20-21 0.632642901026486 1102 22-23 0.8198633194883413 1102 24-25 0.6315125068370975 1102 26-27 0.779922004080646 1102 28-29 0.37518913414989186 1102 30-31 0.677501607370445 1102 32-33 0.91610946526162 1102 34-35 0.9058937526822746 1102 36-37 0.5721854455639246 1102 38-39 -0.014463533531909434 1102 40-41 -0.40222953366798464 1102 42-43 -0.06527209564149672 1102 44-45 -0.3796599539672343 1102 46-47 -0.18152733535441 1102 48-49 -0.5208616243674058 1102 50-51 -0.4923602292580682 1102 52-53 -0.7013027684429574 1102 54-55 -0.6425146637594636 1102 56-57 -0.7048201274461121 1102 58-59 -0.6469884375939472 1102 60-61 -0.6953754611539722 1102 62-63 -0.7158711214914248 1102 64-65 -0.578257442206425 1102 66-67 -0.6946617709254568 1102 68-69 -0.4823930260038054 1102 70-71 -0.9077195900356294 1102 72-73 -0.7568136408472377 1102 74-75 -0.5844552021262643 1102 76-77 -0.9834810170913784 1102 78-79 -0.8326477867804698 1102 80-81 -0.7839788903482194 1102 82-83 -1.0258075077204758 1102 84-85 -0.8919940654301044 1102 86-87 -0.5776876455309221 1102 88-89 -0.7040480700185654 1102 90-91 -0.6714401073920229 1102 92-93 -0.5451628837117752 1102 94-95 -0.4687151830160339 1102 96-97 -0.7131855756759755 1102 98-99 -0.84615378973524 1102 100-101 -0.6986915868717034 1102 102-103 -0.7583594875988737 1102 104-105 -0.6529556852322074 1102 106-107 -0.5640989110577621 1102 108-109 -0.4870118279443574 1102 110-111 -0.4282335209590329 1102 112-113 -0.3385831045481531 1102 114-115 -0.42674884479875175 1102 116-117 -0.5115909916438603 1102 118-119 -0.28691162950631366 1102 120-121 -0.09532185480258804 1102 122-123 -0.0916763620527341 1102 124-125 0.03062977367184061 1103 1 0.2737319527307882 1103 2 0.04437442128577729 1103 3 0.24232277002085212 1103 4 0.2441400483578633 1103 5 0.22340292538994788 1103 6 0.2720051871531268 1103 7 0.36066795909741245 1103 8 0.4180112266720073 1103 9 0.5883973011392456 1103 10-11 0.5529270573814742 1103 12-13 0.5227791025973119 1103 14-15 0.5865445272841754 1103 16-17 0.5603318351276485 1103 18-19 0.6455183872842696 1103 20-21 0.6669253855114121 1103 22-23 0.6397816737027924 1103 24-25 0.49884328779489806 1103 26-27 0.5929380395634141 1103 28-29 0.6538629785629553 1103 30-31 0.5697469852386305 1103 32-33 0.6945236454135539 1103 34-35 0.7017531604102274 1103 36-37 0.7064493594655161 1103 38-39 0.6722819285517119 1103 40-41 0.5538806201223565 1103 42-43 0.6241587591667681 1103 44-45 0.5795811785792253 1103 46-47 0.6596559563030766 1103 48-49 0.6546924700143464 1103 50-51 0.6729294070608063 1103 52-53 0.5921262681756403 1103 54-55 0.6415617088441792 1103 56-57 0.6669356348331448 1103 58-59 0.8304493833424402 1103 60-61 0.8258241553418912 1103 62-63 0.8100232896267414 1103 64-65 0.7556333325324012 1103 66-67 0.6941062652137546 1103 68-69 0.7623224553845844 1103 70-71 0.7006614653781256 1103 72-73 0.6728290049858252 1103 74-75 0.6619486907862502 1103 76-77 0.7374990994282342 1103 78-79 0.627259295521764 1103 80-81 0.6408952183717496 1103 82-83 0.6803830826081381 1103 84-85 0.7285104498128909 1103 86-87 0.7300758300185279 1103 88-89 0.7398632619241354 1103 90-91 0.7036138768618514 1103 92-93 0.632906013152386 1103 94-95 0.647953858957802 1103 96-97 0.7429051437829699 1103 98-99 0.7467904682835815 1103 100-101 0.6756522885067291 1103 102-103 0.5144190603542569 1103 104-105 0.5937710335184683 1103 106-107 0.7728373313790442 1103 108-109 0.8221046009756563 1103 110-111 0.6852923418173091 1103 112-113 0.6167439536555079 1103 114-115 0.6820135553843656 1103 116-117 0.7366353017108942 1103 118-119 0.6359277047166785 1103 120-121 0.6173709229679929 1103 122-123 0.7159280758848077 1103 124-125 0.9956660079030399 1104 1 0.42883696874319455 1104 2 0.6215429364254561 1104 3 0.3751667895554931 1104 4 0.18553620175393348 1104 5 0.33549080985667956 1104 6 0.45026263667242716 1104 7 0.46736700494580674 1104 8 0.3949922161613415 1104 9 0.4737699295640283 1104 10-11 0.5427790806476764 1104 12-13 0.48439635990178687 1104 14-15 0.5193836433794914 1104 16-17 0.54270057129294 1104 18-19 0.5765561281090754 1104 20-21 0.5200972926773559 1104 22-23 0.6382182594585899 1104 24-25 0.702439095230659 1104 26-27 0.5985009090725981 1104 28-29 0.7257782207598993 1104 30-31 0.6064955228599658 1104 32-33 0.7027989615823742 1104 34-35 0.8916318435524531 1104 36-37 0.8851226742069898 1104 38-39 0.9265317476601282 1104 40-41 0.8343047923476234 1104 42-43 0.7469833009218192 1104 44-45 0.72340599723438 1104 46-47 0.6945917253683902 1104 48-49 0.8185376194003027 1104 50-51 0.7023150596797407 1104 52-53 0.7467355629539298 1104 54-55 0.4351410670952234 1104 56-57 0.6190320199682233 1104 58-59 0.7219079422751875 1104 60-61 0.7571513433059707 1104 62-63 0.830598945163004 1104 64-65 0.8221255150491267 1104 66-67 0.8161634599190322 1104 68-69 0.6031651794223123 1104 70-71 0.17336160811353807 1104 72-73 0.1838297681093266 1104 74-75 0.701609026659483 1104 76-77 0.7800264433134423 1104 78-79 0.7941961664187396 1104 80-81 0.8498594471637553 1104 82-83 0.7982935746497333 1104 84-85 0.7279362735494921 1104 86-87 0.7867291791087965 1104 88-89 0.7962296568001648 1104 90-91 0.8537127697559015 1104 92-93 0.7998362499843843 1104 94-95 0.7940210121775095 1104 96-97 0.8113170640027754 1104 98-99 0.8157290961776411 1104 100-101 0.7197321478151046 1104 102-103 0.6096048512584211 1104 104-105 0.7521185621026376 1104 106-107 0.739602499488722 1104 108-109 0.7117758562726095 1104 110-111 0.781249703321933 1104 112-113 0.7787730380556503 1104 114-115 0.7448124385539501 1104 116-117 0.6999198932090742 1104 118-119 0.721132256426948 1104 120-121 0.734503457576384 1104 122-123 0.7644277676686251 1104 124-125 1.1493378598537838 1105 1 0.17682408629553237 1105 2 -0.006476387246042492 1105 3 0.17649529197095504 1105 4 0.18030270738999832 1105 5 0.0029626295024129945 1105 6 0.30291964150334394 1105 7 0.3512236877155317 1105 8 0.45859930150756156 1105 9 0.6058150181308548 1105 10-11 0.4595463914367244 1105 12-13 0.4581282439597558 1105 14-15 0.5118353342007467 1105 16-17 0.5221691703267552 1105 18-19 0.5985971909110006 1105 20-21 0.6349322363166436 1105 22-23 0.6360040887661498 1105 24-25 0.5686830565833176 1105 26-27 0.5829010701031905 1105 28-29 0.6161767714845325 1105 30-31 0.6443377128760588 1105 32-33 0.6790469486999342 1105 34-35 0.6849088161772485 1105 36-37 0.6979246065741407 1105 38-39 0.6824697508807418 1105 40-41 0.46172025128483085 1105 42-43 0.4799744442390548 1105 44-45 0.5423472210669118 1105 46-47 0.4848493743216906 1105 48-49 0.604668859335888 1105 50-51 0.4101451724011582 1105 52-53 0.38411479000706805 1105 54-55 0.41018132474417257 1105 56-57 0.44058999098272267 1105 58-59 0.04215753969869951 1105 60-61 0.1872036782496096 1105 62-63 0.2936625522483567 1105 64-65 0.12898944419566405 1105 66-67 0.3075080653940745 1105 68-69 0.4164703324013814 1105 70-71 0.4488245710765 1105 72-73 0.3864263864668125 1105 74-75 0.35629904276254365 1105 76-77 0.41086380241166864 1105 78-79 0.3118088878358094 1105 80-81 0.3397950349254302 1105 82-83 0.3571703862439328 1105 84-85 0.30965930092469307 1105 86-87 0.42068650600090507 1105 88-89 0.4774897212124003 1105 90-91 0.20868458940163492 1105 92-93 0.40762610505684904 1105 94-95 0.3803736691823403 1105 96-97 0.41155860989649895 1105 98-99 0.28654230068649866 1105 100-101 0.2877675745461836 1105 102-103 -0.06108758030357819 1105 104-105 0.2629881273200283 1105 106-107 0.18374501156601752 1105 108-109 0.1816832636800143 1105 110-111 0.11528754551195064 1105 112-113 0.08271828765307276 1105 114-115 0.1533269313075678 1105 116-117 -0.08530554962506542 1105 118-119 0.04269022744144024 1105 120-121 0.14442696804337274 1105 122-123 0.1678094101806984 1105 124-125 0.6601067809487908 1106 1 -0.08855847004148387 1106 2 -0.499655955480101 1106 3 -0.025159087410855818 1106 4 -0.1985669539162771 1106 5 0.09849202044593852 1106 6 0.2432638439933541 1106 7 0.3201154004411322 1106 8 0.2924746464388832 1106 9 0.3974487563775355 1106 10-11 0.37204048393110867 1106 12-13 0.3705975175767904 1106 14-15 0.36048130204626716 1106 16-17 0.3453891686141546 1106 18-19 0.37753990346763544 1106 20-21 0.36364431920017637 1106 22-23 0.3134382262065998 1106 24-25 0.38621087088468187 1106 26-27 0.46520378474501456 1106 28-29 0.40701330287831183 1106 30-31 0.40317112922496534 1106 32-33 0.3702047201175418 1106 34-35 0.4316135229312863 1106 36-37 0.5922366959289604 1106 38-39 0.5352016413119571 1106 40-41 0.5169747732638541 1106 42-43 0.26612441578824786 1106 44-45 0.3563177038024534 1106 46-47 0.3113356798575566 1106 48-49 0.4545053366388174 1106 50-51 0.5457741272841616 1106 52-53 0.44400137243581383 1106 54-55 0.4611202627070341 1106 56-57 0.08398754045460777 1106 58-59 0.39843272830582777 1106 60-61 0.4281910144531835 1106 62-63 0.327762361966208 1106 64-65 0.41689740934350183 1106 66-67 0.30715671996988547 1106 68-69 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-1.264362802050229 2216 52-53 -1.2053899208353016 2216 54-55 -1.1403806178214815 2216 56-57 -1.0454504050047149 2216 58-59 -1.0453825525768075 2216 60-61 -1.0192114309587055 2216 62-63 -1.221891880924808 2216 64-65 -1.08794269718339 2216 66-67 -0.9546715451241354 2216 68-69 -0.824209885253083 2216 70-71 -0.9055606941206733 2216 72-73 -0.7681224150933943 2216 74-75 -0.81228565030003 2216 76-77 -0.8577675834839589 2216 78-79 -0.8834290255942143 2216 80-81 -0.8498316496188245 2216 82-83 -0.7415996775096865 2216 84-85 -0.6837914252660582 2216 86-87 -0.8693706276487347 2216 88-89 -0.8431567904576411 2216 90-91 -0.8467864998686778 2216 92-93 -0.8679946996704722 2216 94-95 -0.8045185173968683 2216 96-97 -0.8337856602299567 2216 98-99 -0.8417563423309034 2216 100-101 -0.8727535991508688 2216 102-103 -0.8865357818889166 2216 104-105 -0.7627895125506754 2216 106-107 -0.8513221041686485 2216 108-109 -0.8873795096355543 2216 110-111 -0.9102833595754838 2216 112-113 -0.8158681720293117 2216 114-115 -1.0183219519614966 2216 116-117 -1.0010849338261174 2216 118-119 -1.082351385550755 2216 120-121 -1.2741073220688186 2216 122-123 -1.307678164665166 2216 124-125 -1.5895634608041789 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 90309.0 3 31502.0 4 15204.0 5 11934.0 6 12594.0 7 15065.0 8 17131.0 9 18396.0 10 20407.0 11 22613.0 12 25179.0 13 28615.0 14 33422.0 15 29840.0 16 20284.0 17 17567.0 18 16317.0 19 15547.0 20 15368.0 21 15901.0 22 17718.0 23 21996.0 24 28764.0 25 37776.0 26 47776.0 27 54262.0 28 62294.0 29 74630.0 30 86447.0 31 102215.0 32 126210.0 33 157303.0 34 203919.0 35 283814.0 36 465010.0 37 744406.0 38 288463.0 39 811.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.17013905328426 19.79683430259996 14.230976354252162 23.802050289863615 2 15.14173904976735 20.971813312631525 39.61403206827691 24.272415569324213 3 18.72039411548763 25.655346079305858 29.850238433888954 25.774021371317563 4 12.477646974009701 17.264203450262492 37.42047760176392 32.83767197396388 5 13.070852109347273 38.86068057792944 34.163104220952704 13.905363091770576 6 31.053286701379214 37.539567331063175 17.640384875354325 13.766761092203286 7 27.94913539633835 33.0503046291733 21.067888133270877 17.93267184121748 8 25.981608965293727 35.73107902981371 19.681129292050144 18.606182712842415 9 26.69155207366015 15.957370516590899 19.94203645105858 37.40904095869036 10-11 24.72291217733865 27.078629758547834 27.504875290072324 20.693582774041193 12-13 25.51781212685104 24.44785778739088 28.062709013005456 21.971621072752622 14-15 22.702355319242432 25.56687380683843 26.133045753931633 25.5977251199875 16-17 22.010587419448107 29.06235195121151 26.545509196285327 22.381551433055055 18-19 21.819853830298317 27.401974613851408 29.199548542857528 21.578623012992743 20-21 22.828762916878055 26.47408995373002 29.14554255655962 21.55160457283231 22-23 22.50956588767379 26.49036056965361 28.87860546003993 22.12146808263267 24-25 22.13404052292807 26.741717828666324 29.040048409998263 22.08419323840734 26-27 22.136985281395322 27.02017296870408 28.82590496394731 22.01693678595329 28-29 21.851603092942376 26.735416350702728 29.26447562396075 22.148504932394143 30-31 22.08019569064351 26.542267659319823 29.21703907435103 22.160497575685632 32-33 22.0201561851066 26.784134556847416 28.931595854634516 22.264113403411464 34-35 22.264616911335917 26.74490671218785 28.844366921177205 22.146109455299023 36-37 22.369834809730456 26.438819667568808 28.82813260506761 22.36321291763312 38-39 22.189807840015085 26.78285290031245 28.829154878732403 22.19818438094006 40-41 22.308086428813592 26.659462943189965 28.86613982445578 22.166310803540668 42-43 22.261244934023676 26.6102870025685 28.6128753384565 22.51559272495132 44-45 22.378684342948098 26.761049481402093 28.364661189517637 22.49560498613217 46-47 22.390905853477975 27.029403947319036 27.942950416065383 22.63673978313761 48-49 22.577508941843003 27.096156891848633 27.658559985645446 22.667774180662917 50-51 22.567849260010735 27.114162404901794 27.532638776786257 22.785349558301217 52-53 22.756361059734655 27.13881774508401 27.487519869490747 22.61730132569059 54-55 22.58350526348875 27.036269964470648 27.51727261048108 22.86295216155952 56-57 22.50631834767598 27.359624190493893 27.329457256109034 22.804600205721098 58-59 22.584311990870866 27.07820507323739 27.438826688166134 22.89865624772561 60-61 22.728561319178556 27.052504280580248 27.620964727286374 22.59796967295482 62-63 22.594216250245267 27.036056355048153 27.681019490639176 22.6887079040674 64-65 22.50913945682026 26.91184009765029 27.607705218187366 22.97131522734208 66-67 22.44731399883247 27.265823804573014 27.364679193740386 22.922183002854126 68-69 22.37888610908394 27.081589809730428 27.583449966638778 22.956074114546848 70-71 22.328531902109518 27.02089008605103 27.683277647391265 22.96730036444819 72-73 22.554423866397684 27.012803443628012 27.414968954922003 23.0178037350523 74-75 22.452604773014055 27.23677244035258 27.247559861029945 23.06306292560342 76-77 22.52543401620197 27.064969916164404 27.401679397279654 23.00791667035397 78-79 22.44711564722587 27.13927547956078 27.335521507569858 23.078087365643487 80-81 22.300776287630754 27.239658017208995 27.39267874618516 23.066886948975085 82-83 22.502791417417527 27.190236584641664 27.32685506814293 22.980116929797873 84-85 22.44938906179385 27.04811002960321 27.195592078019924 23.30690883058301 86-87 22.30581301424561 26.874994850487138 27.523406252469858 23.295785882797393 88-89 22.4982518114712 26.87178236803675 27.35390552870058 23.27606029179147 90-91 22.32253898639567 27.047747968917385 27.567444515325484 23.06226852936146 92-93 22.35403581518578 26.979228144356888 27.385049561571883 23.281686478885447 94-95 22.273466444553556 26.801558982596628 27.37316253937661 23.551812033473208 96-97 22.440379694915915 26.77733268716186 27.310592003750074 23.471695614172152 98-99 22.131992506293326 26.778893234821016 27.2521173322331 23.836996926652553 100-101 21.963457660252093 26.80097597193234 27.506955043556662 23.728611324258907 102-103 22.264800005126624 26.605908009407358 27.41235986840439 23.716932117061624 104-105 22.267912599568692 26.522646108082093 27.44098353101868 23.76845776133053 106-107 22.014658129060745 26.646033678237067 27.54206422856843 23.797243964133756 108-109 22.051236356079585 26.75633481629132 27.053724905851645 24.13870392177745 110-111 21.91438669943035 26.688047995080872 27.369539693668223 24.02802561182056 112-113 21.949802396026378 26.59196236568163 27.459231878826092 23.999003359465902 114-115 21.795286494483232 26.769853241172058 27.25111527005266 24.18374499429205 116-117 21.673193451711604 26.71820553437601 27.364084138920582 24.2445168749918 118-119 21.63475901347845 26.59444938967211 27.288939395650118 24.481852201199324 120-121 21.491958512341185 26.674192917888128 27.466661702738303 24.36718686703238 122-123 21.654771673814242 26.70070118762758 27.2150031020465 24.429524036511683 124-125 21.493466363350247 26.625453138330823 27.1974622573699 24.68361824094903 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 255.0 1 196.0 2 225.0 3 341.5 4 269.0 5 214.0 6 425.5 7 957.0 8 1657.5 9 2009.5 10 1664.5 11 1256.0 12 1245.0 13 1389.0 14 1607.5 15 1842.0 16 2151.5 17 2510.5 18 2942.0 19 3327.5 20 3783.0 21 4537.5 22 5514.0 23 6751.0 24 8227.0 25 10729.0 26 14797.0 27 19240.5 28 23674.5 29 29012.0 30 36426.0 31 45201.5 32 53508.0 33 61961.0 34 71971.5 35 79703.5 36 86889.5 37 95238.0 38 98921.0 39 100979.5 40 103432.5 41 106482.5 42 109652.5 43 113675.5 44 117547.5 45 118731.5 46 118545.5 47 118722.0 48 122470.5 49 124557.0 50 121394.0 51 116341.5 52 111904.5 53 110281.0 54 107362.0 55 98903.5 56 89768.0 57 80805.5 58 74259.0 59 68796.0 60 60003.0 61 49151.5 62 38421.5 63 29282.5 64 21656.0 65 16079.0 66 11760.0 67 9025.0 68 6806.0 69 4715.0 70 3315.0 71 2479.5 72 1952.0 73 1578.5 74 1229.5 75 930.5 76 635.5 77 322.0 78 197.5 79 130.0 80 84.0 81 57.0 82 39.0 83 30.5 84 22.5 85 17.5 86 11.5 87 6.0 88 3.0 89 3.0 90 3.5 91 2.0 92 0.5 93 1.5 94 1.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09850445940185089 2 3.0515631784960002E-5 3 0.0 4 0.1038446949642189 5 0.0 6 0.0 7 0.00225815675208704 8 0.005950548198067201 9 0.003722907077765121 10-11 7.476329787315201E-4 12-13 0.0 14-15 3.0515631784960002E-5 16-17 1.9835160660224005E-4 18-19 0.0019682582501299203 20-21 0.0014189768780006403 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 6.1031263569920005E-5 52-53 0.0 54-55 0.0 56-57 0.007400040707852802 58-59 8.696955058713602E-4 60-61 0.0 62-63 0.0 64-65 2.7464068606464003E-4 66-67 0.0 68-69 1.5257815892480001E-5 70-71 0.0 72-73 0.0 74-75 0.0013426877985382403 76-77 0.0 78-79 0.0 80-81 6.1031263569920005E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0013884612462156803 90-91 1.5257815892480001E-5 92-93 0.0022886723838720004 94-95 0.0 96-97 0.008330767477294082 98-99 0.0038449696049049605 100-101 0.0021513520408396806 102-103 0.0 104-105 0.0 106-107 0.0017088753799577605 108-109 0.0 110-111 1.9835160660224005E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.00105278929658112 122-123 0.0042721884498944 124-125 0.08423840154238209 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3277009.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.156267828770126 #Duplication Level Percentage of deduplicated Percentage of total 1 72.6032609174397 33.51095556147425 2 12.879334876228238 11.88924060007221 3 5.273812817323772 7.302585506255902 4 2.77444780062014 5.1223262304946156 5 1.6171867809093805 3.7321653094399982 6 1.0646378074149285 2.948382466780682 7 0.7106960124335596 2.296215284630563 8 0.4970952667324454 1.835524981417334 9 0.3866681242680177 1.6062441754106518 >10 1.9540560254746413 16.196955175754116 >50 0.14872209036840248 4.765093528669047 >100 0.08696382645312788 7.285144429960953 >500 0.00258698976653523 0.7910480670093135 >1k 3.9799842538807594E-4 0.2999228571753491 >5k 1.3266614179602534E-4 0.4181958254550932 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7362 0.22465608120087557 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6297 0.19215693334989314 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3701 0.11293835323613696 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.577344767744001E-5 0.0 12-13 0.0 0.0 0.0 6.103126356992001E-5 0.0 14-15 0.0 0.0 0.0 6.103126356992001E-5 0.0 16-17 0.0 0.0 0.0 9.154689535488002E-5 0.0 18-19 0.0 0.0 0.0 1.2206252713984002E-4 0.0 20-21 0.0 0.0 0.0 1.5257815892480003E-4 0.0 22-23 0.0 0.0 0.0 3.2041413374208006E-4 0.0 24-25 0.0 0.0 0.0 6.408282674841601E-4 0.0 26-27 0.0 0.0 0.0 0.0010985627442585601 0.0 28-29 0.0 0.0 0.0 0.0020140316978073604 1.5257815892480003E-5 30-31 0.0 0.0 0.0 0.0045010556882816005 3.0515631784960006E-5 32-33 0.0 0.0 0.0 0.010131189752606722 3.0515631784960006E-5 34-35 0.0 1.5257815892480003E-5 0.0 0.019041754233815045 6.103126356992001E-5 36-37 3.0515631784960006E-5 3.0515631784960006E-5 0.0 0.03298739795954177 6.103126356992001E-5 38-39 3.0515631784960006E-5 3.0515631784960006E-5 0.0 0.05561473892808961 6.103126356992001E-5 40-41 3.0515631784960006E-5 3.0515631784960006E-5 0.0 0.08327715914115585 6.103126356992001E-5 42-43 3.0515631784960006E-5 3.0515631784960006E-5 0.0 0.11161092325349121 6.103126356992001E-5 44-45 3.0515631784960006E-5 3.0515631784960006E-5 0.0 0.14401852420911876 6.103126356992001E-5 46-47 4.577344767744001E-5 3.0515631784960006E-5 0.0 0.18443647850829825 6.103126356992001E-5 48-49 6.103126356992001E-5 3.0515631784960006E-5 0.0 0.22924868378451202 6.103126356992001E-5 50-51 6.103126356992001E-5 3.0515631784960006E-5 0.0 0.2790196792257818 6.103126356992001E-5 52-53 6.103126356992001E-5 3.0515631784960006E-5 0.0 0.32743272905262083 6.103126356992001E-5 54-55 6.103126356992001E-5 3.0515631784960006E-5 0.0 0.3790651780327732 6.103126356992001E-5 56-57 6.103126356992001E-5 3.0515631784960006E-5 0.0 0.4417290279031886 6.103126356992001E-5 58-59 6.103126356992001E-5 3.0515631784960006E-5 0.0 0.5135323094931994 6.103126356992001E-5 60-61 6.103126356992001E-5 3.0515631784960006E-5 0.0 0.5947038900411931 6.103126356992001E-5 62-63 6.103126356992001E-5 3.0515631784960006E-5 0.0 0.6753414470329498 6.103126356992001E-5 64-65 6.103126356992001E-5 3.0515631784960006E-5 0.0 0.7596561376547943 6.103126356992001E-5 66-67 6.103126356992001E-5 3.0515631784960006E-5 0.0 0.8581605970566453 6.103126356992001E-5 68-69 6.103126356992001E-5 3.0515631784960006E-5 0.0 0.9736622633627188 6.103126356992001E-5 70-71 6.103126356992001E-5 3.0515631784960006E-5 0.0 1.1032011202898742 6.103126356992001E-5 72-73 6.103126356992001E-5 3.0515631784960006E-5 0.0 1.2371952594576334 6.103126356992001E-5 74-75 6.103126356992001E-5 3.0515631784960006E-5 0.0 1.3764838607400833 6.103126356992001E-5 76-77 6.103126356992001E-5 3.0515631784960006E-5 0.0 1.5292298556397008 6.103126356992001E-5 78-79 6.103126356992001E-5 3.0515631784960006E-5 0.0 1.7078225906611793 6.103126356992001E-5 80-81 6.103126356992001E-5 3.0515631784960006E-5 0.0 1.910461643529206 6.103126356992001E-5 82-83 6.103126356992001E-5 3.0515631784960006E-5 0.0 2.12298776109556 6.103126356992001E-5 84-85 6.103126356992001E-5 3.0515631784960006E-5 0.0 2.335254495791742 6.103126356992001E-5 86-87 6.103126356992001E-5 3.0515631784960006E-5 0.0 2.560154702046897 6.103126356992001E-5 88-89 6.103126356992001E-5 3.0515631784960006E-5 0.0 2.81543321974398 6.103126356992001E-5 90-91 6.103126356992001E-5 3.0515631784960006E-5 0.0 3.10896308188351 6.103126356992001E-5 92-93 6.103126356992001E-5 3.0515631784960006E-5 0.0 3.424128526958577 6.103126356992001E-5 94-95 6.103126356992001E-5 3.0515631784960006E-5 0.0 3.7492420679955414 9.154689535488002E-5 96-97 6.103126356992001E-5 3.0515631784960006E-5 0.0 4.073485913526634 1.0680471124736003E-4 98-99 6.103126356992001E-5 3.0515631784960006E-5 0.0 4.429328695771052 1.2206252713984002E-4 100-101 6.103126356992001E-5 3.0515631784960006E-5 0.0 4.847347077777327 1.2206252713984002E-4 102-103 6.103126356992001E-5 3.0515631784960006E-5 0.0 5.282362666687824 1.2206252713984002E-4 104-105 6.103126356992001E-5 3.0515631784960006E-5 0.0 5.727295835928434 1.2206252713984002E-4 106-107 6.103126356992001E-5 3.0515631784960006E-5 0.0 6.178576256580314 1.2206252713984002E-4 108-109 6.103126356992001E-5 3.0515631784960006E-5 0.0 6.647067493558913 1.2206252713984002E-4 110-111 6.103126356992001E-5 3.0515631784960006E-5 0.0 7.16864067202745 1.2206252713984002E-4 112-113 6.103126356992001E-5 3.0515631784960006E-5 0.0 7.734644610374888 1.2206252713984002E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6585 0.0 57.588276 1 GTATCAA 11840 0.0 50.35946 1 ATCAACG 15215 0.0 39.190125 3 TCAACGC 15335 0.0 38.844612 4 CAACGCA 15500 0.0 38.500523 5 AACGCAG 15750 0.0 38.00273 6 TATCAAC 15930 0.0 37.16937 2 GTACATG 17130 0.0 34.111553 1 TACATGG 17495 0.0 32.482464 2 ACGCAGA 18920 0.0 31.478231 7 ACATGGG 17420 0.0 31.391285 3 CGCAGAG 19095 0.0 31.03395 8 GCAGAGT 20710 0.0 27.550907 9 GAGTACT 10770 0.0 26.130203 12-13 CATGGGA 11080 0.0 26.128292 4 CATGGGG 7205 0.0 25.712301 4 AGTACTT 11240 0.0 24.87877 12-13 AGAGTAC 19070 0.0 24.256632 10-11 GTACTTT 11790 0.0 23.667719 14-15 CAGAGTA 20020 0.0 23.343336 10-11 >>END_MODULE