FastQCFastQC Report
Thu 26 May 2016
SRR1364540_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364540_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3725974
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA83500.046.886531
GGTATCA49350.046.6586881
ATCAACG95500.040.8698773
TCAACGC94900.040.5640144
CAACGCA96100.040.057495
AACGCAG99500.038.5639236
TATCAAC104750.036.9195482
GTACATG129000.034.407871
ACGCAGA117200.032.333777
TACATGG133600.032.1534652
CGCAGAG119150.031.359878
ACATGGG132950.031.1922933
GCAGAGT132250.026.7688669
CATGGGG78200.023.890534
GAGTACT55750.023.53810712-13
GTATAGG10750.023.2460671
AGAGTAC116850.022.73854410-11
GAGTACA102950.021.4993481
CAGAGTA128800.021.46049510-11
TAACGCA3108.461939E-921.1122194
Produced by FastQC (version 0.11.5)