##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364540_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3725974 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.75074410073715 31.0 28.0 33.0 12.0 34.0 2 27.6428705621671 31.0 28.0 33.0 10.0 34.0 3 27.879829005784796 31.0 28.0 34.0 15.0 34.0 4 31.17918589877439 35.0 32.0 37.0 16.0 37.0 5 31.29464268940148 35.0 32.0 37.0 16.0 37.0 6 31.252276585934307 35.0 32.0 37.0 14.0 37.0 7 31.065357407217547 35.0 32.0 37.0 11.0 37.0 8 31.037309439088947 35.0 32.0 37.0 10.0 37.0 9 32.115602792719436 37.0 32.0 39.0 10.0 39.0 10-11 32.01352814056137 37.0 32.0 39.0 10.0 39.0 12-13 31.927628856240005 37.0 32.0 39.0 8.0 39.0 14-15 32.58571503719564 38.0 31.0 40.0 8.0 41.0 16-17 32.672533410056005 38.0 32.0 40.0 7.5 41.0 18-19 32.44899253188562 38.0 31.0 40.0 2.0 41.0 20-21 32.15754323567475 38.0 30.5 40.0 2.0 41.0 22-23 32.032098721032405 38.0 30.0 40.0 2.0 41.0 24-25 31.842353704024774 38.0 30.0 40.0 2.0 41.0 26-27 31.73625111178983 38.0 30.0 40.0 2.0 41.0 28-29 31.776587813012114 38.0 30.0 40.0 2.0 41.0 30-31 31.718797823065863 38.0 30.0 40.0 2.0 41.0 32-33 31.712062805591238 38.0 30.0 40.0 2.0 41.0 34-35 31.239329635687206 37.0 29.5 40.0 2.0 41.0 36-37 31.10360270898294 37.0 29.5 40.0 2.0 41.0 38-39 30.90706872887465 37.0 28.5 40.0 2.0 41.0 40-41 31.121299960761938 37.0 29.5 40.0 2.0 41.0 42-43 31.0044636650712 37.0 29.0 40.0 2.0 41.0 44-45 30.852564725357716 37.0 29.0 40.0 2.0 41.0 46-47 30.832490779592128 37.0 29.0 40.0 2.0 41.0 48-49 30.700791927157837 37.0 29.0 40.0 2.0 41.0 50-51 30.543816462487392 37.0 28.0 40.0 2.0 41.0 52-53 30.485538814817282 36.5 28.5 40.0 2.0 41.0 54-55 30.51594925246392 36.5 28.0 40.0 2.0 41.0 56-57 30.382450602178118 36.0 28.0 40.0 2.0 41.0 58-59 30.184964924607634 36.0 28.0 40.0 2.0 41.0 60-61 29.98092055929537 36.0 28.0 40.0 2.0 41.0 62-63 29.699814867199827 35.0 28.0 39.0 2.0 41.0 64-65 29.420625855145527 35.0 27.5 39.0 2.0 41.0 66-67 29.17859289946736 35.0 27.0 39.0 2.0 40.0 68-69 29.146909103498842 35.0 28.0 39.0 2.0 40.0 70-71 28.915959424300866 35.0 27.5 38.0 2.0 40.0 72-73 28.633307290925806 34.5 27.0 37.0 2.0 40.0 74-75 28.330898846851856 34.0 26.0 37.0 2.0 39.0 76-77 28.0264979036354 34.0 26.0 36.0 2.0 39.0 78-79 27.725166359185543 34.0 26.0 36.0 2.0 38.5 80-81 27.42726156972647 34.0 26.0 35.5 2.0 37.0 82-83 27.18467842234004 34.0 26.0 35.0 2.0 37.0 84-85 26.944901655245044 34.0 26.0 35.0 2.0 36.5 86-87 26.751540134203836 34.0 25.0 35.0 2.0 36.0 88-89 26.563082297407334 33.5 25.0 35.0 2.0 36.0 90-91 26.4253528070781 33.0 25.0 35.0 2.0 35.5 92-93 26.305510854343055 33.0 24.5 35.0 2.0 35.0 94-95 26.18422458127727 33.0 24.0 35.0 2.0 35.0 96-97 26.07035328212167 33.0 24.0 35.0 2.0 35.0 98-99 25.978620355375533 33.0 23.5 35.0 2.0 35.0 100-101 25.88632757501797 33.0 23.0 35.0 2.0 35.0 102-103 24.760142717045262 31.5 19.5 34.0 2.0 35.0 104-105 25.60705912064872 33.0 21.5 35.0 2.0 35.0 106-107 25.75021551411792 33.0 23.0 35.0 2.0 35.0 108-109 25.70701164313009 33.0 21.5 35.0 2.0 35.0 110-111 25.62333218106192 33.0 20.0 35.0 2.0 35.0 112-113 25.534445489957793 33.0 19.5 35.0 2.0 35.0 114-115 25.412689138464195 33.0 19.0 35.0 2.0 35.0 116-117 25.296848823958513 33.0 18.0 35.0 2.0 35.0 118-119 25.147164875546636 33.0 16.0 35.0 2.0 35.0 120-121 24.966147240963036 33.0 4.5 35.0 2.0 35.0 122-123 24.72547460073527 33.0 2.0 35.0 2.0 35.0 124-125 23.31129216145899 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7990440091683517 1101 2 0.8088285620609881 1101 3 0.7739482328828267 1101 4 0.8286835887544015 1101 5 0.7843004626734107 1101 6 0.873121582246597 1101 7 0.9152242159011301 1101 8 0.8428414037256928 1101 9 1.2241050090397536 1101 10-11 1.2502098832862956 1101 12-13 1.0335156334682125 1101 14-15 1.3012665984321643 1101 16-17 1.0270637437091494 1101 18-19 1.4838235270826985 1101 20-21 1.7451806980307794 1101 22-23 1.66775982251486 1101 24-25 1.8778545665267465 1101 26-27 1.89361038767683 1101 28-29 1.668767946651446 1101 30-31 1.7781129669306743 1101 32-33 1.7240900634430645 1101 34-35 2.1265816221724485 1101 36-37 2.013910984091609 1101 38-39 1.7545489028579944 1101 40-41 1.6937421019154613 1101 42-43 1.667489200665699 1101 44-45 1.6475852757830545 1101 46-47 1.4898943703917062 1101 48-49 1.5027787934188304 1101 50-51 1.4191129565261953 1101 52-53 1.239287649120925 1101 54-55 1.2073435850784797 1101 56-57 1.2265085614801947 1101 58-59 1.2325052003900439 1101 60-61 1.2008808526590755 1101 62-63 1.1667263241304298 1101 64-65 1.3126280109010544 1101 66-67 1.1141058147844767 1101 68-69 0.9627219746886482 1101 70-71 0.9797994936609662 1101 72-73 0.8545250089334502 1101 74-75 0.8100183210467016 1101 76-77 0.9912242282061072 1101 78-79 0.9901128067326077 1101 80-81 1.112121717945275 1101 82-83 0.8659003773775744 1101 84-85 1.000980184142648 1101 86-87 1.037544123035964 1101 88-89 1.0371690129904323 1101 90-91 1.0095030926321265 1101 92-93 0.8755018418618121 1101 94-95 0.9647615591349137 1101 96-97 0.9168438531758412 1101 98-99 0.9735896681666532 1101 100-101 1.0955311647797288 1101 102-103 0.9756219185289083 1101 104-105 1.0405624297925335 1101 106-107 0.981920065576503 1101 108-109 1.035546439536791 1101 110-111 0.8294753086471651 1101 112-113 0.8463291617982414 1101 114-115 0.9644014234128555 1101 116-117 1.0152839700505076 1101 118-119 0.9716723321691703 1101 120-121 1.040460443956178 1101 122-123 1.1309415458416865 1101 124-125 1.3351177889363903 1102 1 0.07043450420969322 1102 2 0.31078479389125846 1102 3 0.49715238949815443 1102 4 0.6279300867647528 1102 5 0.7368440226000139 1102 6 -0.1549209240934779 1102 7 -0.7148277170487063 1102 8 0.10189823141996968 1102 9 0.4993357877865847 1102 10-11 0.5686030875501231 1102 12-13 0.6950535976339012 1102 14-15 0.9311376691317435 1102 16-17 0.7201786073202072 1102 18-19 0.924748324509558 1102 20-21 1.3076351628871734 1102 22-23 1.1294014381516533 1102 24-25 1.2681898915715841 1102 26-27 0.8344567702256427 1102 28-29 1.4563908822610223 1102 30-31 1.315649166184297 1102 32-33 1.214588766562592 1102 34-35 1.5206349571552344 1102 36-37 1.6849271033468796 1102 38-39 1.691843523587469 1102 40-41 1.5146837072431865 1102 42-43 1.272869064998396 1102 44-45 1.3111513053494512 1102 46-47 0.9861568639485405 1102 48-49 0.6098280171461745 1102 50-51 1.0266448919502054 1102 52-53 0.8990276792234013 1102 54-55 0.8635404140098402 1102 56-57 0.8316401010360792 1102 58-59 1.0570124888995593 1102 60-61 0.9173371401583026 1102 62-63 0.7315873867889309 1102 64-65 0.7980865375293718 1102 66-67 0.8150698856283753 1102 68-69 0.6110157748164831 1102 70-71 0.6379176685439596 1102 72-73 0.843337940625581 1102 74-75 0.435761559330448 1102 76-77 0.631579255937492 1102 78-79 0.5308218395360562 1102 80-81 0.2082017492848358 1102 82-83 0.5010790334877591 1102 84-85 0.08896962765927441 1102 86-87 0.540732263486678 1102 88-89 0.7855073647769899 1102 90-91 0.21869839994537799 1102 92-93 0.6382146436597154 1102 94-95 0.5775874060246693 1102 96-97 0.7283129115457747 1102 98-99 0.7311215788489065 1102 100-101 0.7562022437265554 1102 102-103 0.5707567487385958 1102 104-105 0.873849017697939 1102 106-107 0.8176025298555913 1102 108-109 0.3997666741711292 1102 110-111 0.6120770364465926 1102 112-113 0.4085053808452699 1102 114-115 0.4648460987803702 1102 116-117 0.4564296948809279 1102 118-119 0.7655047397483976 1102 120-121 1.0615431597764697 1102 122-123 0.9287603619485694 1102 124-125 1.1984085417043708 1103 1 0.5907465677655956 1103 2 0.5768637740846074 1103 3 0.5342465331154251 1103 4 0.5803818532024252 1103 5 0.6271798328648615 1103 6 0.619163020786587 1103 7 0.8684460444930053 1103 8 0.8957404554344066 1103 9 0.9942162990737486 1103 10-11 1.1037262905147784 1103 12-13 1.013064604661622 1103 14-15 1.2580988045513308 1103 16-17 1.2228941248148857 1103 18-19 1.215891947193633 1103 20-21 1.6548704457495091 1103 22-23 1.5767990066265085 1103 24-25 1.6961709737552262 1103 26-27 1.658012892597199 1103 28-29 1.5997037133360017 1103 30-31 1.5898225393049792 1103 32-33 1.5221230568229203 1103 34-35 1.918134887515091 1103 36-37 1.852701322255868 1103 38-39 1.871072359648128 1103 40-41 1.5899137247460189 1103 42-43 1.44790845276804 1103 44-45 1.13476079913249 1103 46-47 1.2959456854749014 1103 48-49 1.2822745171618912 1103 50-51 1.196402203260849 1103 52-53 1.251339322049894 1103 54-55 1.1449198241194516 1103 56-57 1.0464848005211707 1103 58-59 1.0354381043084544 1103 60-61 0.9830296449296085 1103 62-63 0.868488642971009 1103 64-65 1.170909635520843 1103 66-67 1.1776945784273458 1103 68-69 1.1674609977697017 1103 70-71 1.2560697387853175 1103 72-73 1.2206055242313525 1103 74-75 1.1471232056414422 1103 76-77 1.1613385241445577 1103 78-79 1.1478983139789847 1103 80-81 1.1973617251021942 1103 82-83 1.209468099689257 1103 84-85 1.1948903648547606 1103 86-87 1.1629497412145327 1103 88-89 1.1572616234753106 1103 90-91 1.1679504714118742 1103 92-93 1.1989211297487365 1103 94-95 1.1212134467713426 1103 96-97 1.2978800313830376 1103 98-99 1.2277877000149289 1103 100-101 1.2635419001527808 1103 102-103 1.1661858118908697 1103 104-105 1.150585546629177 1103 106-107 1.2321495878524011 1103 108-109 1.3643097961300974 1103 110-111 1.359553963147075 1103 112-113 1.3642900134711695 1103 114-115 1.422852094373674 1103 116-117 1.3988916279499541 1103 118-119 1.371947587134283 1103 120-121 1.4189931331671275 1103 122-123 1.4535440865466427 1103 124-125 1.5462588159537418 1104 1 0.7500693114687387 1104 2 0.6871985661239073 1104 3 0.6460231762921929 1104 4 0.7365593176610901 1104 5 0.7291674969373467 1104 6 0.6714082848991012 1104 7 0.7646365323443334 1104 8 0.8188071668769688 1104 9 1.091925079850597 1104 10-11 0.9898214993599694 1104 12-13 1.021150311433658 1104 14-15 1.0534104953501569 1104 16-17 1.075932279317712 1104 18-19 1.3031998344714069 1104 20-21 1.5553350501889582 1104 22-23 1.3533956690526807 1104 24-25 1.7826066937540865 1104 26-27 1.7535075533084097 1104 28-29 1.6819143857947516 1104 30-31 1.614454375795571 1104 32-33 1.5700070483715791 1104 34-35 1.823294714439367 1104 36-37 1.8927542831803592 1104 38-39 1.8898370178579036 1104 40-41 1.5589210753517193 1104 42-43 1.6410315923696075 1104 44-45 1.525179100148943 1104 46-47 1.5098004667021847 1104 48-49 1.441104732242902 1104 50-51 1.3374056955127855 1104 52-53 1.3834336510169578 1104 54-55 1.277538194941691 1104 56-57 1.2322499974312073 1104 58-59 1.1492934837107995 1104 60-61 1.1832957113275988 1104 62-63 1.1508777896826956 1104 64-65 1.1682665273558683 1104 66-67 1.1125390920108558 1104 68-69 1.1696125119610805 1104 70-71 1.1204839056020752 1104 72-73 0.9853178968936689 1104 74-75 1.0034598750157855 1104 76-77 1.0890064635844396 1104 78-79 1.0990819014759659 1104 80-81 1.0691324014827224 1104 82-83 1.0467311472036407 1104 84-85 1.0508517572654839 1104 86-87 0.9944759627635555 1104 88-89 1.004886636377357 1104 90-91 1.1155579845778796 1104 92-93 1.0540009460853135 1104 94-95 1.10728127976099 1104 96-97 0.9265942662390891 1104 98-99 1.0439501533950484 1104 100-101 1.0551031267817805 1104 102-103 1.0297154584905996 1104 104-105 1.0693730320716384 1104 106-107 1.033853480168176 1104 108-109 1.069302068611801 1104 110-111 1.1596650430097704 1104 112-113 1.107815523939589 1104 114-115 1.083511961846117 1104 116-117 1.064401651479109 1104 118-119 1.0160774475753662 1104 120-121 1.0860276774965278 1104 122-123 1.0538959599109887 1104 124-125 1.4100982985422839 1105 1 0.30224178224135656 1105 2 0.46550445008733377 1105 3 0.5784588891989877 1105 4 0.7402074027589514 1105 5 0.7226205128576844 1105 6 0.6693954942672988 1105 7 0.7527921293332618 1105 8 0.6304204517928156 1105 9 0.5166756982893403 1105 10-11 0.7144999640516048 1105 12-13 0.6919241177464173 1105 14-15 0.9204977910927212 1105 16-17 0.846109615268773 1105 18-19 1.038985512497895 1105 20-21 1.3177959258203202 1105 22-23 1.499906376266921 1105 24-25 1.5627472035533252 1105 26-27 1.2929475005130229 1105 28-29 1.132070698945025 1105 30-31 1.003066279876645 1105 32-33 1.0548853900328865 1105 34-35 1.6806565378785585 1105 36-37 1.6308370720709178 1105 38-39 1.81905954349158 1105 40-41 1.3736520368327376 1105 42-43 0.9913648691358645 1105 44-45 1.1299108462370633 1105 46-47 1.105093272610791 1105 48-49 0.9688560458300266 1105 50-51 0.989027423710386 1105 52-53 0.9708197278475019 1105 54-55 0.7189198573281423 1105 56-57 0.7252052916613287 1105 58-59 0.7583841308377828 1105 60-61 0.7608717724426555 1105 62-63 0.7583815581930011 1105 64-65 0.8654918076547595 1105 66-67 0.514827229353596 1105 68-69 0.4621372338433609 1105 70-71 0.7039345050308405 1105 72-73 0.7841786590549873 1105 74-75 0.7414123773471815 1105 76-77 0.8144934668166286 1105 78-79 0.8406449481895244 1105 80-81 0.8061276616447977 1105 82-83 0.7023819895472592 1105 84-85 0.627370288431834 1105 86-87 0.7287743433762799 1105 88-89 0.8503992490133285 1105 90-91 0.7420577699629511 1105 92-93 0.6760370727878673 1105 94-95 0.7395223369911044 1105 96-97 0.8210022786443609 1105 98-99 0.6670336107683923 1105 100-101 0.8228403469329457 1105 102-103 0.834757274915642 1105 104-105 0.9373218903116296 1105 106-107 0.8377975532264657 1105 108-109 0.8139700888742034 1105 110-111 0.8158843261332471 1105 112-113 0.8628645965978947 1105 114-115 0.8860395948235009 1105 116-117 0.984953961425088 1105 118-119 0.9825695171452544 1105 120-121 0.9002991799398679 1105 122-123 1.001927290372386 1105 124-125 1.0429076736693936 1106 1 0.53373999125861 1106 2 0.7427121958538478 1106 3 0.42554215299594134 1106 4 0.6404301187414418 1106 5 0.3838883500305492 1106 6 0.43088814286872434 1106 7 0.2159470154769494 1106 8 0.5259570394037887 1106 9 0.6763750691316588 1106 10-11 0.8218954730117574 1106 12-13 0.7552858231701052 1106 14-15 1.0942207164950872 1106 16-17 1.0054504527936245 1106 18-19 0.9290141951632904 1106 20-21 1.364056390265958 1106 22-23 1.3848223887927062 1106 24-25 1.5215040539781377 1106 26-27 1.7327437445756146 1106 28-29 1.460036946297965 1106 30-31 1.3516358136373974 1106 32-33 1.2660178588949336 1106 34-35 1.5389711119803913 1106 36-37 1.4597473927165652 1106 38-39 1.6857857120956545 1106 40-41 1.390436528685818 1106 42-43 1.2836757919229171 1106 44-45 1.1912098781160303 1106 46-47 0.9382090163207693 1106 48-49 1.0511377895305287 1106 50-51 1.0925331049885507 1106 52-53 1.0921372308341368 1106 54-55 0.8624595035420093 1106 56-57 0.8860346130885368 1106 58-59 0.8554798675280182 1106 60-61 0.9624982439634238 1106 62-63 0.8754699084358357 1106 64-65 0.9678758016398312 1106 66-67 0.8680107046159833 1106 68-69 0.883569294118363 1106 70-71 0.9922992226582572 1106 72-73 0.9296507306254469 1106 74-75 0.9757958160390423 1106 76-77 0.8670496553299429 1106 78-79 0.8044318358359455 1106 80-81 0.9101588570992796 1106 82-83 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-2.7443576890897674 2216 96-97 -2.7464578073506125 2216 98-99 -2.750018597090431 2216 100-101 -2.772527354094745 2216 102-103 -2.6324416406573796 2216 104-105 -2.8471832480332964 2216 106-107 -2.827650076983165 2216 108-109 -2.9086234360572085 2216 110-111 -2.9194912352152933 2216 112-113 -2.9743491872860695 2216 114-115 -3.145519618821112 2216 116-117 -3.218945556352395 2216 118-119 -3.239250706967997 2216 120-121 -3.3677241254282 2216 122-123 -3.4783445025324937 2216 124-125 -3.448584533664924 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 298386.0 3 47597.0 4 28939.0 5 22569.0 6 20818.0 7 21845.0 8 22839.0 9 23869.0 10 24505.0 11 25781.0 12 27673.0 13 28933.0 14 31014.0 15 27796.0 16 22608.0 17 20441.0 18 18779.0 19 18007.0 20 17891.0 21 19352.0 22 23252.0 23 30285.0 24 40473.0 25 50859.0 26 61673.0 27 72035.0 28 82149.0 29 95544.0 30 108820.0 31 125534.0 32 151351.0 33 189841.0 34 242319.0 35 326516.0 36 499467.0 37 648066.0 38 207643.0 39 505.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.16222767114794 18.600941348890956 13.111994212865103 24.124836767096006 2 15.059096223402943 19.836340661358083 40.750976706135724 24.353586409103258 3 19.335509044123235 24.76971311806519 28.64232264776434 27.252455190047236 4 12.283666950373341 16.283152681699097 37.74503922640678 33.68814114152078 5 13.843221393351996 37.6917066241408 34.33675514083114 14.128316841676064 6 31.933991457730688 36.33813945574032 17.89614545798083 13.831723628548161 7 28.591819857899804 31.88064141830715 21.147194298169918 18.380344425623125 8 25.83931074129879 35.76095807431828 19.882183826296156 18.517547358086773 9 26.24672930406676 16.589651489886037 19.72790923186386 37.43570997418334 10-11 24.872623909882353 26.665202172639958 27.72591810892937 20.736255808548314 12-13 25.706594271504674 23.8123943612829 27.869108974535322 22.611902392677102 14-15 22.724437651759967 25.33972556737475 25.512116705203237 26.423720075662043 16-17 22.72893479839004 28.655912130961635 25.869707389573072 22.745445681075253 18-19 22.584445647183678 27.574428213131057 27.699771952231423 22.141354187453842 20-21 23.240350815270673 26.76152133397948 27.890715870954025 22.107411979795817 22-23 23.04195268820321 26.632817122415673 27.569423168821384 22.755807020559736 24-25 22.759907981236033 26.737568159499908 27.79211199866221 22.71041186060185 26-27 22.72323517355734 26.96216874397524 27.609883140822916 22.7047129416445 28-29 22.581057698733506 26.946835403629187 27.748132895790263 22.72397400184704 30-31 22.89249479726092 26.853648573426923 27.45876054302801 22.795096086284147 32-33 22.699148886428766 26.90786583865869 27.474693331912555 22.918291942999993 34-35 22.93595736225881 26.746639048888383 27.654313598047253 22.663089990805556 36-37 22.929343481092666 26.639961895574388 27.58380873274735 22.846885890585593 38-39 22.737880081825583 26.832970385730015 27.75710458527086 22.672044947173546 40-41 23.035819606139768 26.767631482939585 27.451210420756684 22.745338490163963 42-43 22.86902088801404 26.765528448999763 27.324363784625078 23.041086878361114 44-45 22.987294718429094 26.853961402991217 27.22447786987525 22.93426600870444 46-47 23.0093219847679 27.0656280396026 26.86004891277508 23.065001062854417 48-49 23.09781189828545 27.081012044861353 26.77662543883845 23.044550618014743 50-51 22.974381877597665 27.137439159358095 26.798023897829726 23.090155065214514 52-53 23.196203194117835 27.119982587103397 26.648535389672606 23.03527882910616 54-55 23.154579865283946 26.895644048546743 26.74180479137749 23.207971294791825 56-57 23.032266809096548 27.183270215067328 26.784122110359892 23.000340865476232 58-59 23.14568072737169 26.992578831298108 26.738308995553634 23.123431445776568 60-61 23.248904565149502 26.859333832286108 26.91165811616206 22.980103486402328 62-63 23.149830494642085 26.935485949364235 26.87081404451489 23.043869511478793 64-65 23.070207097172773 26.875186980154904 26.828855495562337 23.225750427109983 66-67 23.039024194514813 27.10318951911434 26.64924629751176 23.208539988859087 68-69 22.90746297023579 26.964006293388852 26.912116169451185 23.21641456692417 70-71 22.86410001435908 26.890252330666836 26.955284209170223 23.29036344580386 72-73 23.10402514549992 26.88226534971316 26.703431134063926 23.31027837072299 74-75 22.974063020040266 26.981941766359853 26.730155219837176 23.313839993762702 76-77 23.07083962905152 26.89349637716898 26.767306900778138 23.268357093001363 78-79 22.980069858890623 26.96289083598915 26.730962754494534 23.32607655062569 80-81 22.900326140650186 27.066305603303842 26.78389998084999 23.249468275195987 82-83 23.041593636575787 27.15755859930914 26.71828436397629 23.082563400138785 84-85 23.111540767595265 26.92663716923414 26.699166446142677 23.262655617027924 86-87 23.023012410519705 26.886864807506566 26.886515884517763 23.203606897455963 88-89 23.145093489553695 26.820251918216236 26.808481167873175 23.22617342435689 90-91 23.029072487803802 27.026563049895884 26.816127836372196 23.12823662592812 92-93 23.108432072806952 27.002497183153785 26.77383475969443 23.11523598434483 94-95 22.99387819969031 26.963330405406854 26.767453734824358 23.275337660078474 96-97 23.07229360359381 26.908821985909842 26.69172508485905 23.327159325637297 98-99 22.891339751751502 26.81878739991798 26.6897596957979 23.60011315253262 100-101 22.86239755467911 26.83121262728665 26.835762173861927 23.47062764417231 102-103 22.99406812822634 26.744027199330965 26.852898061017065 23.40900661142563 104-105 22.998402680887363 26.72221073366774 26.946530537807938 23.33285604763696 106-107 22.83095218765034 26.77160123967635 26.971642973747123 23.425803598926187 108-109 22.791449967176366 26.80534002652729 26.758090636166543 23.645119370129798 110-111 22.716876349321034 26.877669790345976 26.890767128258364 23.51468673207463 112-113 22.880835990804016 26.82771001622663 26.8882176848255 23.403236308143857 114-115 22.81352473205664 26.886741560730158 26.774421936384957 23.525311770828246 116-117 22.707498764081553 26.902603185100055 26.839773975878522 23.55012407493987 118-119 22.73741040597707 26.82592524800227 26.77787069904406 23.6587936469766 120-121 22.6907008329501 26.918723388666077 26.85151947538006 23.539056303003765 122-123 22.74688443880714 26.963822077126682 26.75376961836019 23.535523865705986 124-125 22.82523842325759 26.894419958884303 26.571891531444276 23.708450086413833 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 273.0 1 311.0 2 390.0 3 427.0 4 385.5 5 334.0 6 347.0 7 454.5 8 659.5 9 813.5 10 860.5 11 926.0 12 1044.5 13 1186.0 14 1340.5 15 1495.0 16 1740.0 17 1977.0 18 2154.5 19 2478.0 20 2956.0 21 3650.0 22 4631.5 23 5862.0 24 7522.5 25 10257.5 26 14155.0 27 18859.5 28 23819.5 29 29797.5 30 37681.5 31 45579.0 32 52776.5 33 60534.5 34 69396.5 35 77873.0 36 86781.0 37 95457.5 38 101603.0 39 105977.5 40 109978.5 41 116215.5 42 121653.0 43 127617.0 44 134919.5 45 138182.5 46 140030.5 47 142261.0 48 146163.0 49 147330.0 50 144127.0 51 140513.0 52 136811.5 53 133771.5 54 129307.0 55 121729.5 56 112803.0 57 104188.0 58 96094.0 59 86110.0 60 73111.5 61 58780.5 62 45684.5 63 35463.5 64 27074.5 65 20446.5 66 15432.0 67 11815.0 68 8964.5 69 6707.5 70 4836.0 71 3449.5 72 2544.0 73 1951.0 74 1522.0 75 1111.5 76 753.0 77 505.0 78 349.5 79 231.0 80 157.0 81 114.0 82 90.5 83 77.5 84 65.5 85 49.5 86 34.0 87 37.5 88 31.5 89 19.5 90 18.0 91 18.5 92 15.5 93 12.0 94 9.0 95 8.0 96 10.0 97 7.0 98 6.5 99 7.5 100 9.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012587312740239198 2 0.0049919833042313235 3 0.007917392874990539 4 0.007917392874990539 5 0.014895434052948303 6 8.588358372871094E-4 7 0.007863715635160095 8 0.0 9 0.0022812826927938843 10-11 0.0 12-13 0.003395085419275604 14-15 0.01944458012857846 16-17 0.03107912186182727 18-19 0.005891077071391266 20-21 0.00830655286376126 22-23 0.0019994771836840514 24-25 0.011527187253587921 26-27 0.019350644958875184 28-29 4.5625653855877683E-4 30-31 0.0013956082355915528 32-33 0.008735970782404815 34-35 0.009514290759946257 36-37 0.011339316914181366 38-39 0.0 40-41 0.025416173059715393 42-43 0.009890031438759368 44-45 0.017002265716293243 46-47 0.004240501946605102 48-49 0.02526856065018167 50-51 0.004146566776901825 52-53 0.0 54-55 0.017632973284300964 56-57 0.004401533666096436 58-59 2.952248190674439E-4 60-61 0.00457598469554538 62-63 0.027321715073696167 64-65 0.01732432915527591 66-67 0.0018116068442774963 68-69 0.021336702832601622 70-71 0.0029254095707592163 72-73 0.01225182999129892 74-75 1.3419309957611084E-5 76-77 0.006105786030713043 78-79 9.259323870751648E-4 80-81 0.003421924039190826 82-83 0.0014895434052948303 84-85 0.0 86-87 0.005998431551052155 88-89 0.017123039505911743 90-91 0.022463924869040953 92-93 0.004723597105079102 94-95 0.02485256204149573 96-97 0.018223422922435853 98-99 8.051585974566651E-4 100-101 0.008803067332192872 102-103 0.0 104-105 0.0011674799663121643 106-107 4.025792987283325E-5 108-109 0.0 110-111 6.978041177957764E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 1.3419309957611084E-5 122-123 0.0 124-125 0.023510631045734618 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3725974.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.106299810421326 #Duplication Level Percentage of deduplicated Percentage of total 1 77.92685376991926 45.2804112843779 2 11.540504151630044 13.41151988396055 3 4.292768271475017 7.483106405969743 4 2.0503357308256946 4.765496907495086 5 1.1523268024539557 3.3478723331486853 6 0.7024120221290132 2.4488738128963643 7 0.47633837319864325 1.9374782227002114 8 0.32882031436673925 1.5285225416280595 9 0.2216845883795773 1.1593144040160206 >10 1.1996199027662167 12.356817611429825 >50 0.07805540029471383 3.0976099118577105 >100 0.02976544254401613 2.849629442223877 >500 3.747127449691704E-4 0.13017634599794414 >1k 1.4051727206879235E-4 0.20317089229803476 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 2.683861991522217E-5 2.683861991522217E-5 2 1.3419309957611085E-4 0.0 0.0 2.683861991522217E-5 2.683861991522217E-5 3 1.3419309957611085E-4 0.0 0.0 2.683861991522217E-5 2.683861991522217E-5 4 1.3419309957611085E-4 0.0 0.0 2.683861991522217E-5 2.683861991522217E-5 5 1.8787033940655516E-4 0.0 0.0 2.683861991522217E-5 5.367723983044434E-5 6 1.8787033940655516E-4 0.0 0.0 2.683861991522217E-5 5.367723983044434E-5 7 2.415475792369995E-4 0.0 0.0 2.683861991522217E-5 5.367723983044434E-5 8 2.683861991522217E-4 0.0 0.0 2.683861991522217E-5 5.367723983044434E-5 9 2.683861991522217E-4 0.0 0.0 8.05158597456665E-5 5.367723983044434E-5 10-11 3.086441290250549E-4 0.0 0.0 8.05158597456665E-5 5.367723983044434E-5 12-13 3.623213688554993E-4 0.0 0.0 9.39351697032776E-5 8.05158597456665E-5 14-15 4.5625653855877683E-4 0.0 0.0 1.0735447966088867E-4 8.05158597456665E-5 16-17 5.367723983044433E-4 0.0 0.0 1.3419309957611085E-4 8.05158597456665E-5 18-19 7.112234277533874E-4 0.0 0.0 1.476124095337219E-4 8.05158597456665E-5 20-21 8.05158597456665E-4 0.0 0.0 2.549668891946106E-4 9.39351697032776E-5 22-23 8.319972173718872E-4 0.0 0.0 3.489020588978882E-4 1.0735447966088867E-4 24-25 0.0010332868667360536 0.0 0.0 4.83095158473999E-4 1.0735447966088867E-4 26-27 0.0010735447966088868 0.0 0.0 6.307075680077209E-4 1.3419309957611085E-4 28-29 0.001274834445973053 0.0 0.0 0.0011674799663121643 1.3419309957611085E-4 30-31 0.0014090275455491638 0.0 0.0 0.002750958541310272 1.61031719491333E-4 32-33 0.001435866165464386 0.0 2.683861991522217E-5 0.006293656370119598 1.8787033940655516E-4 34-35 0.0016371558148285522 0.0 2.683861991522217E-5 0.01140641346396942 1.8787033940655516E-4 36-37 0.0019860578737264404 0.0 2.683861991522217E-5 0.020249738726035124 1.8787033940655516E-4 38-39 0.0022812826927938843 0.0 2.683861991522217E-5 0.03617845964571948 1.8787033940655516E-4 40-41 0.0023349599326243286 0.0 2.683861991522217E-5 0.05485813910671411 1.8787033940655516E-4 42-43 0.0024557337222428284 0.0 2.683861991522217E-5 0.07439665440499585 1.8787033940655516E-4 44-45 0.0026704426815646054 0.0 2.683861991522217E-5 0.09635064549564758 2.0128964936416626E-4 46-47 0.0031266992201233823 0.0 2.683861991522217E-5 0.1264098998006964 2.1470895932177735E-4 48-49 0.003327988869487549 0.0 2.683861991522217E-5 0.15728773201315951 2.415475792369995E-4 50-51 0.003327988869487549 0.0 2.683861991522217E-5 0.19197664825358418 2.415475792369995E-4 52-53 0.003327988869487549 0.0 2.683861991522217E-5 0.22620930795545002 2.8180550910983273E-4 54-55 0.003327988869487549 0.0 2.683861991522217E-5 0.2645482765043449 2.952248190674438E-4 56-57 0.0036500523084702146 0.0 2.683861991522217E-5 0.30963715796191815 2.952248190674438E-4 58-59 0.0036500523084702146 0.0 2.683861991522217E-5 0.36415981431969197 2.952248190674438E-4 60-61 0.0036500523084702146 0.0 2.683861991522217E-5 0.42284245676432525 2.952248190674438E-4 62-63 0.003703729548300659 0.0 2.683861991522217E-5 0.48455786325937866 2.952248190674438E-4 64-65 0.0037574067881311034 0.0 2.683861991522217E-5 0.5492657758749793 2.952248190674438E-4 66-67 0.003998954367368103 0.0 2.683861991522217E-5 0.620562569679767 2.952248190674438E-4 68-69 0.004106308847028992 0.0 2.683861991522217E-5 0.7031852610887783 2.952248190674438E-4 70-71 0.004240501946605102 0.0 2.683861991522217E-5 0.7994822293445956 2.952248190674438E-4 72-73 0.004428372286011657 0.0 2.683861991522217E-5 0.8975505465148174 3.22063438982666E-4 74-75 0.004602823315460601 0.0 2.683861991522217E-5 1.0002351063104573 3.757406788131103E-4 76-77 0.004629661935375824 0.0 2.683861991522217E-5 1.1131854382236699 3.757406788131103E-4 78-79 0.004710177795121491 0.0 2.683861991522217E-5 1.2450704164870716 3.757406788131103E-4 80-81 0.005233530883468323 0.0 2.683861991522217E-5 1.3925352136112599 3.757406788131103E-4 82-83 0.005273788813341156 0.0 2.683861991522217E-5 1.5503999759525966 3.757406788131103E-4 84-85 0.005394562602959656 0.0 2.683861991522217E-5 1.7117403395729545 3.757406788131103E-4 86-87 0.005501917082620544 0.0 2.683861991522217E-5 1.888392135854947 3.757406788131103E-4 88-89 0.005542175012493378 0.0 2.683861991522217E-5 2.0858438625712363 3.757406788131103E-4 90-91 0.0059044963813488765 0.0 2.683861991522217E-5 2.304417583160806 3.757406788131103E-4 92-93 0.006011850861009765 0.0 2.683861991522217E-5 2.5460859361874237 3.757406788131103E-4 94-95 0.006011850861009765 0.0 2.683861991522217E-5 2.7921826615000533 3.757406788131103E-4 96-97 0.006011850861009765 0.0 2.683861991522217E-5 3.045914974178564 4.5625653855877683E-4 98-99 0.006320494990034821 1.3419309957611084E-5 2.683861991522217E-5 3.3256539095549247 4.5625653855877683E-4 100-101 0.006615719809102264 2.683861991522217E-5 2.683861991522217E-5 3.6388203460356943 4.5625653855877683E-4 102-103 0.006978041177957764 2.683861991522217E-5 2.683861991522217E-5 3.977362697646307 4.5625653855877683E-4 104-105 0.00819919838410037 8.05158597456665E-5 2.683861991522217E-5 4.325862177245467 4.5625653855877683E-4 106-107 0.008467584583252592 9.39351697032776E-5 2.683861991522217E-5 4.676750294017081 4.5625653855877683E-4 108-109 0.008601777682828705 1.3419309957611085E-4 2.683861991522217E-5 5.047673440555409 4.5625653855877683E-4 110-111 0.008735970782404815 1.3419309957611085E-4 2.683861991522217E-5 5.457351554251318 4.5625653855877683E-4 112-113 0.00891042181185376 1.3419309957611085E-4 2.683861991522217E-5 5.9036107068916746 4.5625653855877683E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8350 0.0 46.88653 1 GGTATCA 4935 0.0 46.658688 1 ATCAACG 9550 0.0 40.869877 3 TCAACGC 9490 0.0 40.564014 4 CAACGCA 9610 0.0 40.05749 5 AACGCAG 9950 0.0 38.563923 6 TATCAAC 10475 0.0 36.919548 2 GTACATG 12900 0.0 34.40787 1 ACGCAGA 11720 0.0 32.33377 7 TACATGG 13360 0.0 32.153465 2 CGCAGAG 11915 0.0 31.35987 8 ACATGGG 13295 0.0 31.192293 3 GCAGAGT 13225 0.0 26.768866 9 CATGGGG 7820 0.0 23.89053 4 GAGTACT 5575 0.0 23.538107 12-13 GTATAGG 1075 0.0 23.246067 1 AGAGTAC 11685 0.0 22.738544 10-11 GAGTACA 10295 0.0 21.499348 1 CAGAGTA 12880 0.0 21.460495 10-11 TAACGCA 310 8.461939E-9 21.112219 4 >>END_MODULE