FastQCFastQC Report
Thu 26 May 2016
SRR1364540_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364540_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3725974
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47410.1272418970180683No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41190.11054827543080012No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA99650.046.1087951
GGTATCA59900.044.7705461
ATCAACG121700.037.5300253
TCAACGC122700.036.98154
CAACGCA123950.036.5300875
AACGCAG127800.035.569286
TATCAAC130000.035.0427362
GTACATG131250.031.0572831
ACGCAGA153550.029.3718957
TACATGG135750.029.3471662
CGCAGAG155400.028.7159258
ACATGGG135350.028.4215893
GCAGAGT174900.024.7989229
AGAGTAC155050.022.85022510-11
GAGTACT86950.022.4100412-13
CATGGGG77800.021.584584
AGTACTT98150.021.1258112-13
GTACTTT97250.020.37302614-15
CATGGGA74450.019.9163044
CAGAGTA170550.019.8491110-11
Produced by FastQC (version 0.11.5)