##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364540_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3725974 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.043058539860986 31.0 30.0 34.0 26.0 34.0 2 29.97477545468648 31.0 30.0 34.0 26.0 34.0 3 30.259404654997592 31.0 30.0 34.0 26.0 34.0 4 33.80422139284923 35.0 35.0 37.0 29.0 37.0 5 33.22727265407649 35.0 33.0 37.0 27.0 37.0 6 33.239402636733374 36.0 33.0 37.0 27.0 37.0 7 33.0355193031406 36.0 33.0 37.0 26.0 37.0 8 32.96561543370942 36.0 33.0 37.0 26.0 37.0 9 34.239984498013136 38.0 34.0 39.0 25.0 39.0 10-11 34.16814489312057 38.0 34.0 39.0 25.0 39.0 12-13 34.05449018162767 38.0 34.0 39.0 25.0 39.0 14-15 35.02877032958362 39.0 34.0 41.0 25.0 41.0 16-17 35.021320599660655 39.0 34.0 41.0 25.0 41.0 18-19 35.013038469941016 39.0 34.0 41.0 25.0 41.0 20-21 35.01051483451039 39.0 34.0 41.0 25.0 41.0 22-23 34.962960154848105 39.0 34.0 41.0 24.5 41.0 24-25 34.915438218302114 39.0 34.0 41.0 24.0 41.0 26-27 34.793439648263785 39.0 34.0 41.0 24.0 41.0 28-29 34.65229507774343 39.0 34.0 41.0 23.5 41.0 30-31 34.532390993603286 38.5 33.5 41.0 23.0 41.0 32-33 34.37242181507439 38.0 33.0 41.0 22.5 41.0 34-35 34.21545507295542 38.0 33.0 40.0 21.5 41.0 36-37 34.038830115293344 38.0 33.0 40.0 19.5 41.0 38-39 33.89039483367302 38.0 33.0 40.0 18.5 41.0 40-41 33.94574693757927 38.0 33.0 40.0 18.5 41.0 42-43 33.84369160922755 38.0 33.0 41.0 17.0 41.0 44-45 33.720423572467226 38.0 33.0 40.5 15.5 41.0 46-47 33.54062226413818 38.0 33.0 40.0 15.0 41.0 48-49 33.319778130496886 38.0 32.0 40.0 11.5 41.0 50-51 33.16678511444256 38.0 32.0 40.0 9.0 41.0 52-53 32.990985310149775 38.0 32.0 40.0 8.5 41.0 54-55 32.80314248032863 38.0 32.0 40.0 7.5 41.0 56-57 32.589596706793984 37.5 31.0 40.0 7.0 41.0 58-59 32.381715894957935 37.0 31.0 40.0 4.0 41.0 60-61 32.14506797417266 37.0 31.0 40.0 2.0 41.0 62-63 31.967923420829024 36.0 31.0 40.0 2.0 41.0 64-65 31.830704132664373 36.0 31.0 39.5 2.0 41.0 66-67 31.52861332365712 36.0 31.0 39.0 2.0 41.0 68-69 31.23519487790307 35.0 30.0 39.0 2.0 41.0 70-71 30.915311137436817 35.0 30.0 38.5 2.0 40.0 72-73 30.617564561642137 35.0 30.0 37.5 2.0 40.0 74-75 30.254699844926456 35.0 30.0 37.0 2.0 39.0 76-77 29.93217370813645 35.0 29.5 36.5 2.0 39.0 78-79 29.643762677893083 35.0 29.0 36.0 2.0 39.0 80-81 29.359662735166694 34.0 29.0 36.0 2.0 37.0 82-83 29.07178029154256 34.0 29.0 35.0 2.0 37.0 84-85 28.851267078084817 34.0 29.0 35.0 2.0 36.5 86-87 28.637231365543613 34.0 29.0 35.0 2.0 36.0 88-89 28.464050205395957 34.0 29.0 35.0 2.0 36.0 90-91 28.320766999447663 34.0 29.0 35.0 2.0 36.0 92-93 28.214151789572337 34.0 29.0 35.0 2.0 35.0 94-95 28.103439932753155 34.0 29.0 35.0 2.0 35.0 96-97 27.987404635673787 34.0 28.0 35.0 2.0 35.0 98-99 27.904285832375642 34.0 28.0 35.0 2.0 35.0 100-101 27.807532741774367 34.0 27.5 35.0 2.0 35.0 102-103 26.528897142062718 32.0 25.0 34.0 2.0 35.0 104-105 27.472671441078226 33.5 27.0 35.0 2.0 35.0 106-107 27.637594894650366 34.0 27.0 35.0 2.0 35.0 108-109 27.644240539520673 34.0 27.0 35.0 2.0 35.0 110-111 27.56756998304336 34.0 27.0 35.0 2.0 35.0 112-113 27.485968098542823 34.0 27.0 35.0 2.0 35.0 114-115 27.383919479846078 34.0 27.0 35.0 2.0 35.0 116-117 27.256216092758564 34.0 27.0 35.0 2.0 35.0 118-119 27.116934927618928 34.0 27.0 35.0 2.0 35.0 120-121 26.91734792030218 34.0 26.5 35.0 2.0 35.0 122-123 26.619822628928702 34.0 25.0 35.0 2.0 35.0 124-125 24.785667318129434 31.5 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.761150656440492 1101 2 0.8293145883501545 1101 3 0.6623870234633173 1101 4 0.5736548924591673 1101 5 0.7016590151730426 1101 6 0.6637460949313905 1101 7 0.7825211666907208 1101 8 0.843319078750774 1101 9 1.0126101014080646 1101 10-11 0.9914772465595476 1101 12-13 0.9149008245108803 1101 14-15 1.0547449470771468 1101 16-17 1.102016481572221 1101 18-19 1.1655113235817751 1101 20-21 1.1829957381569756 1101 22-23 1.192853118302864 1101 24-25 1.1407513288907793 1101 26-27 1.1285403725275387 1101 28-29 1.2493881084238865 1101 30-31 1.2720492750960588 1101 32-33 1.333778261821898 1101 34-35 1.3128009131248746 1101 36-37 1.3582420730733133 1101 38-39 1.334597647988275 1101 40-41 1.0910360491503752 1101 42-43 1.2226005550731642 1101 44-45 1.1892803406282226 1101 46-47 1.2716618539358109 1101 48-49 1.347508855018738 1101 50-51 1.2594711552528324 1101 52-53 1.2563985765308274 1101 54-55 1.333867371818009 1101 56-57 1.3532228993407358 1101 58-59 1.3298770352151905 1101 60-61 1.237290727187343 1101 62-63 1.2332103402205874 1101 64-65 1.1640646631015557 1101 66-67 1.2378711986830169 1101 68-69 1.3068201174935545 1101 70-71 1.2969019812077178 1101 72-73 1.265382288230711 1101 74-75 1.2817195759611266 1101 76-77 1.2916827348886457 1101 78-79 1.2213691747538142 1101 80-81 1.0736396250758737 1101 82-83 1.1906317368060577 1101 84-85 1.2298655005959311 1101 86-87 1.2389451589103508 1101 88-89 1.2397436083331357 1101 90-91 1.233858836867011 1101 92-93 1.2249956697237465 1101 94-95 1.2626318719519638 1101 96-97 1.2552340561034683 1101 98-99 1.2106537238278072 1101 100-101 1.17086167826902 1101 102-103 1.0833953249558697 1101 104-105 1.1147184448561127 1101 106-107 1.0923505959012196 1101 108-109 1.1523827737381822 1101 110-111 1.143839831842456 1101 112-113 1.0923598414865765 1101 114-115 1.0824142399951846 1101 116-117 1.126321064533787 1101 118-119 1.0487140206101166 1101 120-121 1.03011926805436 1101 122-123 0.9708694833754414 1101 124-125 1.2910979891958867 1102 1 0.40120653982434007 1102 2 -0.22366388219684552 1102 3 0.3070012293559792 1102 4 0.35863153211607823 1102 5 0.6308609367829092 1102 6 0.48651652853070004 1102 7 0.5499901095991149 1102 8 0.6465265012781458 1102 9 1.0174768051873642 1102 10-11 1.0184566985296115 1102 12-13 1.0814288218717536 1102 14-15 1.186386801884261 1102 16-17 1.235079783359815 1102 18-19 1.2596127235529124 1102 20-21 1.1395623244129354 1102 22-23 1.2966667633823477 1102 24-25 1.242673252150098 1102 26-27 1.3189704749996523 1102 28-29 0.9184525483323398 1102 30-31 1.2091495740589622 1102 32-33 1.354183086464694 1102 34-35 1.3763197849007085 1102 36-37 0.991733555723151 1102 38-39 0.3661323709836779 1102 40-41 -0.028601731513937523 1102 42-43 0.3072709907342883 1102 44-45 0.08150529269106954 1102 46-47 0.22205591591391993 1102 48-49 -0.04634496242832142 1102 50-51 -0.055684425044482566 1102 52-53 -0.2951763600684174 1102 54-55 -0.22576694500020267 1102 56-57 -0.16930478462454346 1102 58-59 -0.20922874817071602 1102 60-61 -0.2948646840412934 1102 62-63 -0.315339702046586 1102 64-65 -0.26541553730668 1102 66-67 -0.2930946123311813 1102 68-69 -0.04867043321673847 1102 70-71 -0.577126496555632 1102 72-73 -0.39651871999592103 1102 74-75 -0.24742243760560356 1102 76-77 -0.6460906553507293 1102 78-79 -0.44242635935888686 1102 80-81 -0.44999944215985366 1102 82-83 -0.7240929575301429 1102 84-85 -0.5643198729015211 1102 86-87 -0.2999799931483693 1102 88-89 -0.4481403198332963 1102 90-91 -0.39615690713826623 1102 92-93 -0.31148943367618287 1102 94-95 -0.07722419699506489 1102 96-97 -0.33522347673090636 1102 98-99 -0.40337907549591634 1102 100-101 -0.29343294658407615 1102 102-103 -0.28403371774428265 1102 104-105 -0.1927379457337679 1102 106-107 -0.10488748166418915 1102 108-109 -0.09638300016338519 1102 110-111 -0.03308097391413156 1102 112-113 0.119856066306685 1102 114-115 0.0812918219007095 1102 116-117 -0.12070091027085894 1102 118-119 0.19409449523950784 1102 120-121 0.38820635079492405 1102 122-123 0.30064613952685804 1102 124-125 0.5348297555512218 1103 1 0.3884159275086638 1103 2 -0.09669779311343518 1103 3 0.2465685228370873 1103 4 0.2674333859284843 1103 5 0.3599867303278117 1103 6 0.41987477626973657 1103 7 0.5246569333752831 1103 8 0.5695893954442823 1103 9 0.8014023719394601 1103 10-11 0.7927486010057834 1103 12-13 0.7273935065649084 1103 14-15 0.7599126693888323 1103 16-17 0.7241107381476368 1103 18-19 0.865201429146282 1103 20-21 0.8580367114974692 1103 22-23 0.9483081907666389 1103 24-25 0.883970652562283 1103 26-27 0.9208262197274024 1103 28-29 0.9732745997461194 1103 30-31 0.8876768292202328 1103 32-33 1.0098994999682631 1103 34-35 0.9255336381203989 1103 36-37 0.9021614862062535 1103 38-39 0.9835220351773941 1103 40-41 0.8716536909467649 1103 42-43 0.98233775314079 1103 44-45 0.977974847695684 1103 46-47 0.9952534803448287 1103 48-49 0.9238989069063805 1103 50-51 0.8639959002877262 1103 52-53 0.7337104391180489 1103 54-55 0.7519500341905285 1103 56-57 0.866453745645984 1103 58-59 1.0283035158020581 1103 60-61 0.92925809165326 1103 62-63 0.7663149385387129 1103 64-65 0.7699317591831516 1103 66-67 0.7623157862657806 1103 68-69 0.813815859128912 1103 70-71 0.7660354934639315 1103 72-73 0.7052085183255414 1103 74-75 0.7624325478702332 1103 76-77 0.8640390061357408 1103 78-79 0.733392649436226 1103 80-81 0.7102047351134466 1103 82-83 0.7825706167488029 1103 84-85 0.8733532085937838 1103 86-87 0.8867205749060574 1103 88-89 0.8899855836417743 1103 90-91 0.8846138198693367 1103 92-93 0.7508149576106682 1103 94-95 0.756321476532392 1103 96-97 0.7975151081700282 1103 98-99 0.8476322172971216 1103 100-101 0.8313608015468859 1103 102-103 0.6821314137016223 1103 104-105 0.6805667898194372 1103 106-107 0.7165738916607474 1103 108-109 0.7906359138348016 1103 110-111 0.7221357702929794 1103 112-113 0.7682413229680591 1103 114-115 0.8437995325340992 1103 116-117 0.8157039237214754 1103 118-119 0.620078772801925 1103 120-121 0.6422435121051748 1103 122-123 0.5951647777035838 1103 124-125 0.9583840153186287 1104 1 0.2170742890753914 1104 2 0.43914403095661214 1104 3 0.20662993173812438 1104 4 -0.0672330677629418 1104 5 0.25559692605972373 1104 6 0.41327509835585374 1104 7 0.44699181501621865 1104 8 0.36486837354443935 1104 9 0.40080210326539145 1104 10-11 0.4771213117983919 1104 12-13 0.42052337563592346 1104 14-15 0.5146598038109502 1104 16-17 0.5383234523772558 1104 18-19 0.46504010257842765 1104 20-21 0.4414462576299627 1104 22-23 0.4517376966301043 1104 24-25 0.557410109628691 1104 26-27 0.45121773433999124 1104 28-29 0.7031066991720394 1104 30-31 0.5712366761833678 1104 32-33 0.5636266337529534 1104 34-35 0.7365512874936684 1104 36-37 0.7998766194776294 1104 38-39 0.8275507541851894 1104 40-41 0.7588688891294808 1104 42-43 0.706860612727894 1104 44-45 0.6370689296351344 1104 46-47 0.6761826967009199 1104 48-49 0.6486126528789313 1104 50-51 0.5241301681574342 1104 52-53 0.707942833307186 1104 54-55 0.3263686197139961 1104 56-57 0.4603882910252963 1104 58-59 0.5559123013362637 1104 60-61 0.5533524990061807 1104 62-63 0.58234286483993 1104 64-65 0.6160469719993529 1104 66-67 0.6470105644810005 1104 68-69 0.3784933351304858 1104 70-71 0.001293367823304692 1104 72-73 -0.03620359603418777 1104 74-75 0.40017215397881145 1104 76-77 0.5601539868475527 1104 78-79 0.6258785020508135 1104 80-81 0.6242569795363764 1104 82-83 0.6143172128445187 1104 84-85 0.5909455091087104 1104 86-87 0.5693271582525448 1104 88-89 0.5360614290617534 1104 90-91 0.5446854818647857 1104 92-93 0.5133569984911333 1104 94-95 0.492823788681509 1104 96-97 0.5053625294139721 1104 98-99 0.5568162482536785 1104 100-101 0.538708076783152 1104 102-103 0.4983853224407291 1104 104-105 0.5612518323616769 1104 106-107 0.5464178081596209 1104 108-109 0.49885663370297095 1104 110-111 0.45464657241551976 1104 112-113 0.48207974784600793 1104 114-115 0.46923721675916497 1104 116-117 0.5051475857093202 1104 118-119 0.4940426486984215 1104 120-121 0.5103359414925563 1104 122-123 0.616249389347928 1104 124-125 0.9041076976332114 1105 1 0.04774606144323812 1105 2 -0.13015192149066834 1105 3 0.1508133388400914 1105 4 0.11964062130728337 1105 5 -0.04623816980303985 1105 6 0.38629109610758405 1105 7 0.4251137801934277 1105 8 0.49028760380370784 1105 9 0.5797213693450161 1105 10-11 0.5890026288225485 1105 12-13 0.6419433399974324 1105 14-15 0.6668663930114391 1105 16-17 0.6324453688928529 1105 18-19 0.7504549446905315 1105 20-21 0.7256177094578504 1105 22-23 0.7150574466961928 1105 24-25 0.72859502057117 1105 26-27 0.695175768120734 1105 28-29 0.7294738294307024 1105 30-31 0.7795086807261029 1105 32-33 0.8754930112926118 1105 34-35 0.8856552532801274 1105 36-37 0.9956851217677425 1105 38-39 1.04250926094209 1105 40-41 0.6974693589598289 1105 42-43 0.7378108898963447 1105 44-45 0.7955735422278494 1105 46-47 0.7654838722844417 1105 48-49 0.7580956189174302 1105 50-51 0.6390871358064274 1105 52-53 0.8011089976082246 1105 54-55 0.6588911309335117 1105 56-57 0.6613134976313333 1105 58-59 0.4335009825217071 1105 60-61 0.5356668896589198 1105 62-63 0.7164180717347364 1105 64-65 0.5305386221700132 1105 66-67 0.7265469298835256 1105 68-69 0.7450686394099684 1105 70-71 0.8123324211042089 1105 72-73 0.8335813472756435 1105 74-75 0.7616713206486523 1105 76-77 0.7278807588830034 1105 78-79 0.6375895464310695 1105 80-81 0.6926543510227106 1105 82-83 0.68414603148182 1105 84-85 0.6391590472124129 1105 86-87 0.7233977269335625 1105 88-89 0.7264761261720736 1105 90-91 0.5775304592303776 1105 92-93 0.6419021231072648 1105 94-95 0.6119788804989774 1105 96-97 0.609923744804167 1105 98-99 0.5432292433134158 1105 100-101 0.5632508572857233 1105 102-103 0.301430061880513 1105 104-105 0.5217911179526382 1105 106-107 0.5216039480536558 1105 108-109 0.5530754421299378 1105 110-111 0.46693965149273 1105 112-113 0.500337039008727 1105 114-115 0.49230091763966044 1105 116-117 0.3277549702814504 1105 118-119 0.37489767935707974 1105 120-121 0.37302641147159576 1105 122-123 0.32383292727099544 1105 124-125 0.7555948291551147 1106 1 -0.08849647446560027 1106 2 -0.5446792375011462 1106 3 0.04235205233099748 1106 4 -0.13230433930706198 1106 5 0.25564850498098224 1106 6 0.35760088460972383 1106 7 0.41238500550302604 1106 8 0.4936169932081853 1106 9 0.522917630570312 1106 10-11 0.5466739945270263 1106 12-13 0.5554137030309647 1106 14-15 0.557778904894981 1106 16-17 0.5338131588433441 1106 18-19 0.5574453403132509 1106 20-21 0.634814048513995 1106 22-23 0.5975658789956952 1106 24-25 0.6027464272765428 1106 26-27 0.7771435067327985 1106 28-29 0.8579097061615997 1106 30-31 0.79667272272183 1106 32-33 0.7004087319526846 1106 34-35 0.7496426239767615 1106 36-37 0.7572034021648619 1106 38-39 0.7625577281108136 1106 40-41 0.7598738010061581 1106 42-43 0.6237304714158043 1106 44-45 0.6801391599990367 1106 46-47 0.5513294396551487 1106 48-49 0.6454294394419762 1106 50-51 0.7621505943950595 1106 52-53 0.5506035959723192 1106 54-55 0.6800688564415509 1106 56-57 0.27209265308175645 1106 58-59 0.6795948153495175 1106 60-61 0.6335149289076227 1106 62-63 0.5213313252565257 1106 64-65 0.6363811000050603 1106 66-67 0.5062989587867079 1106 68-69 0.5972187978281873 1106 70-71 0.5987185948521123 1106 72-73 0.5953564133632661 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-1.1286711975156472 2216 78-79 -1.1099890824943017 2216 80-81 -0.9802881660501868 2216 82-83 -0.8865795184665153 2216 84-85 -0.8976009524982018 2216 86-87 -1.0711469549238117 2216 88-89 -0.9874267477990664 2216 90-91 -0.999780674051884 2216 92-93 -0.9961136260208221 2216 94-95 -0.9658190223830196 2216 96-97 -1.0589259737966863 2216 98-99 -1.09168485271125 2216 100-101 -1.1628600580756867 2216 102-103 -1.0230334219072823 2216 104-105 -0.9477573733936566 2216 106-107 -1.0428075011674984 2216 108-109 -1.048916558847644 2216 110-111 -1.0526172133815308 2216 112-113 -0.9735583815899354 2216 114-115 -1.1042584921314678 2216 116-117 -1.067855410451532 2216 118-119 -1.0837098354419936 2216 120-121 -1.2647770745159832 2216 122-123 -1.2397317019521275 2216 124-125 -1.6336188883736575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 120352.0 3 39653.0 4 19134.0 5 14676.0 6 15039.0 7 17453.0 8 19746.0 9 21495.0 10 23052.0 11 24721.0 12 26860.0 13 29182.0 14 32103.0 15 28943.0 16 22091.0 17 19973.0 18 18847.0 19 18178.0 20 17688.0 21 18595.0 22 21195.0 23 26277.0 24 34256.0 25 44885.0 26 55034.0 27 64158.0 28 74226.0 29 88660.0 30 103074.0 31 121872.0 32 148074.0 33 183705.0 34 237282.0 35 325060.0 36 529257.0 37 824147.0 38 296152.0 39 879.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.808410323641375 19.487713340837594 14.09982457224373 24.6040517632773 2 15.42687384660969 20.68018761278936 39.07858137420249 24.81435716639846 3 19.24380557776452 24.667221152702933 29.158101789787526 26.930871479745022 4 13.052854191914292 17.059708024373123 36.65757998354242 33.229857800170166 5 13.695398840678974 38.04199921953293 34.185665278394325 14.076936661393772 6 32.24126094277631 36.88114839233983 17.31364738454965 13.563943280334215 7 28.45472049452723 32.01087951320244 20.96273600325505 18.571663989015278 8 25.76528462280493 36.1324924082155 19.52952676758269 18.572696201396877 9 25.953852017263223 16.2703980847272 19.581275631803834 38.19447426620574 10-11 24.998758708165443 26.742364444892953 27.45085356594292 20.80802328099868 12-13 25.75439334788702 23.754822229033266 27.897899985346115 22.592884437733595 14-15 22.697422672180934 25.233150602467035 25.519505449786724 26.549921275565314 16-17 22.67094689781203 28.53329082087952 26.034704547839528 22.761057733468924 18-19 22.518007382104066 27.368724758632307 27.942693079059367 22.17057478020426 20-21 23.280784470128122 26.590455695747288 27.940816907675913 22.187942926448677 22-23 22.988056277365327 26.445501230013953 27.746355717994813 22.82008677462591 24-25 22.789611521712175 26.64355682567833 27.834601100276064 22.73223055233343 26-27 22.768892107137624 26.81776630754804 27.730547770864746 22.682793814449592 28-29 22.60680026215964 26.77248955575106 27.923732157014513 22.696978025074785 30-31 22.843114310513172 26.629493388842757 27.783902947256205 22.743489353387865 32-33 22.63696687094435 26.659431869358187 27.82277868820341 22.880822571494058 34-35 22.94124972423318 26.686820680981672 27.677219433093196 22.69471016169195 36-37 22.92405958817748 26.535262994320412 27.67512602073981 22.865551396762296 38-39 22.75739175850395 26.735331486478437 27.800703923323137 22.706572831694476 40-41 22.96747105589035 26.551258811789886 27.757896324558356 22.72337380776141 42-43 22.86286753477077 26.531451910292446 27.590222046638008 23.01545850829877 44-45 22.927978026685103 26.660371221055218 27.471769797642175 22.939880954617504 46-47 22.969148469635055 26.88709046278906 27.169298551197617 22.974462516378267 48-49 23.055649341621816 26.905032080202385 26.994565716239567 23.044752861936235 50-51 22.95851202277205 26.95639606427425 26.978497685569746 23.10659422738395 52-53 23.19368347627808 26.966898717878696 26.801156798350824 23.038261007492405 54-55 23.09814829625757 26.819349786123038 26.88114570847784 23.201356209141554 56-57 22.959995673278293 27.09643019960493 26.949276032363983 22.99429809475279 58-59 23.073195580508482 26.92589190029171 26.949791851686644 23.05112066751316 60-61 23.15795816073864 26.821201650897187 27.041680913500738 22.97915927486343 62-63 23.074288763152936 26.883131766351564 27.02352458712809 23.01905488336741 64-65 23.027434626693505 26.773818283121976 26.955838169774204 23.242908920410315 66-67 22.923241010270065 27.01223894745374 26.832795934700567 23.23172410757563 68-69 22.825608237096727 26.877167386881222 27.034562515616727 23.262661860405323 70-71 22.818637489150486 26.75109917567863 27.05485867587911 23.37540465929177 72-73 22.99406812822634 26.81182155323682 26.81415651316944 23.3799538053674 74-75 22.914461678189305 26.920767223937453 26.76422897767844 23.400542120194803 76-77 22.99516851164286 26.829521623070907 26.82478460665587 23.350525258630363 78-79 22.888525255409725 26.89123702956596 26.785479447790028 23.434758267234283 80-81 22.791122473997046 26.979883496125375 26.85884120660644 23.370152823271145 82-83 22.939290504979372 27.010642049568784 26.7945106433915 23.255556802060347 84-85 22.96427726012044 26.821590810885958 26.78333235819681 23.43079957079679 86-87 22.88211082524999 26.802803776945304 26.96252040406079 23.35256499374392 88-89 22.973108074287442 26.75532903508048 26.859517718047766 23.41204517258431 90-91 22.817365716637543 26.91861603417201 26.928385293132116 23.33563295605833 92-93 22.977166047710902 26.872548074974656 26.851358558028544 23.298927319285895 94-95 22.78070415691362 26.837107134551548 26.850875348415652 23.531313360119174 96-97 22.84208397410338 26.795262209210957 26.766810878232196 23.595842938453462 98-99 22.715906172106553 26.66903838943294 26.786434640429025 23.82862079803148 100-101 22.627915892971235 26.728554249575705 26.89413860372627 23.749391253726788 102-103 22.80760681636533 26.653889694345693 26.85073755211389 23.687765937175083 104-105 22.8071103018969 26.601755675160376 26.937104231001076 23.65402979194165 106-107 22.58407233554939 26.690890703716015 26.989287325108325 23.73574963562627 108-109 22.598510161169962 26.680409831148825 26.73695881089868 23.98412119678253 110-111 22.518670326030886 26.676155928422318 26.86492576690179 23.940247978645 112-113 22.58998586678275 26.692872789772554 26.875281470026362 23.841859873418333 114-115 22.515052439979453 26.74888498953562 26.746241385473972 23.989821185010953 116-117 22.422029783353292 26.70002528197996 26.809835495363092 24.068109439303655 118-119 22.413817165659232 26.653352921947388 26.763740165658696 24.169089746734677 120-121 22.333017306336174 26.70759585313534 26.829927152757787 24.129459687770694 122-123 22.385111922271726 26.73492941113318 26.699312899243115 24.180645767351976 124-125 22.36586952019442 26.64065444322282 26.59352514023956 24.3999508963432 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 118.0 1 98.5 2 130.5 3 191.0 4 167.0 5 160.0 6 273.0 7 552.5 8 949.0 9 1146.5 10 979.0 11 802.0 12 820.0 13 933.5 14 1141.5 15 1371.5 16 1602.0 17 1880.0 18 2179.0 19 2594.0 20 3185.5 21 3880.0 22 4824.0 23 6096.0 24 7847.0 25 10382.0 26 14245.0 27 19114.0 28 24034.0 29 30056.5 30 37588.5 31 45378.0 32 52708.0 33 60083.5 34 69131.0 35 77502.5 36 85794.0 37 94380.0 38 100705.0 39 105255.5 40 109277.5 41 115538.0 42 120793.0 43 126835.0 44 134101.5 45 137664.5 46 139841.5 47 142342.0 48 147458.0 49 149314.0 50 145632.5 51 141452.0 52 138377.5 53 136093.0 54 132067.5 55 124520.5 56 114959.5 57 105695.0 58 97148.0 59 86986.0 60 73559.0 61 58397.0 62 44588.0 63 34340.0 64 26045.5 65 19359.5 66 14503.5 67 10962.0 68 8048.0 69 5826.5 70 4089.0 71 2838.5 72 2036.0 73 1582.5 74 1227.5 75 906.5 76 577.0 77 298.5 78 176.0 79 86.0 80 54.5 81 47.0 82 33.0 83 23.0 84 14.5 85 9.5 86 10.5 87 8.5 88 5.0 89 3.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09779993097106957 2 5.367723983044434E-5 3 2.683861991522217E-5 4 0.09868560542827191 5 0.0 6 0.0 7 0.0019323806338959963 8 0.0063339142999924315 9 0.0035963750686397703 10-11 4.5625653855877683E-4 12-13 0.0 14-15 6.709654978805542E-5 16-17 1.878703394065552E-4 18-19 0.0019189613239383849 20-21 0.0012077378961849975 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 8.05158597456665E-5 52-53 1.3419309957611084E-5 54-55 0.0 56-57 0.007769780465456818 58-59 6.709654978805542E-4 60-61 0.0 62-63 0.0 64-65 2.0128964936416628E-4 66-67 0.0 68-69 2.683861991522217E-5 70-71 0.0 72-73 0.0 74-75 0.001274834445973053 76-77 0.0 78-79 0.0 80-81 9.39351697032776E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.00111380272648172 90-91 1.3419309957611084E-5 92-93 0.0020128964936416624 94-95 1.3419309957611084E-5 96-97 0.00841390734342215 98-99 0.003515859208894104 100-101 0.00210683166334494 102-103 0.0 104-105 0.0 106-107 0.001623736504870941 108-109 1.3419309957611084E-5 110-111 2.1470895932177735E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 7.112234277533875E-4 122-123 0.004669919865248657 124-125 0.08687661266557416 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3725974.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.769844045528025 #Duplication Level Percentage of deduplicated Percentage of total 1 74.03453687408638 39.067909648284335 2 13.213893390966913 13.945901869511152 3 5.11480696016254 8.097226968322756 4 2.508225787690667 5.294347345896328 5 1.393048047075344 3.6755464096046637 6 0.8735730166039039 2.7658987109141684 7 0.5629158542620353 2.079348728811395 8 0.39235379396507397 1.6563558814566552 9 0.30320363139134554 1.4400007508303163 >10 1.4557814816052348 13.857804168438353 >50 0.10391413506783628 3.761276545456073 >100 0.04272754543072791 3.7538022979748202 >500 7.646112786689245E-4 0.243516150064333 >1k 2.5487041340723605E-4 0.36106452443464776 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4741 0.1272418970180683 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4119 0.11054827543080012 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.367723983044434E-5 0.0 0.0 0.0 0.0 3 5.367723983044434E-5 0.0 0.0 0.0 0.0 4 8.05158597456665E-5 0.0 0.0 2.683861991522217E-5 0.0 5 1.61031719491333E-4 0.0 0.0 5.367723983044434E-5 0.0 6 2.1470895932177735E-4 0.0 0.0 5.367723983044434E-5 0.0 7 2.952248190674438E-4 0.0 0.0 5.367723983044434E-5 0.0 8 2.952248190674438E-4 0.0 0.0 5.367723983044434E-5 0.0 9 2.952248190674438E-4 0.0 0.0 2.683861991522217E-4 0.0 10-11 3.22063438982666E-4 0.0 0.0 2.683861991522217E-4 0.0 12-13 3.891599887707214E-4 0.0 0.0 2.683861991522217E-4 1.3419309957611084E-5 14-15 4.83095158473999E-4 0.0 0.0 2.952248190674438E-4 4.025792987283325E-5 16-17 5.099337783892212E-4 0.0 0.0 3.22063438982666E-4 5.367723983044434E-5 18-19 7.112234277533874E-4 0.0 0.0 3.354827489402771E-4 6.709654978805542E-5 20-21 7.783199775414428E-4 0.0 0.0 3.891599887707215E-4 8.05158597456665E-5 22-23 8.05158597456665E-4 0.0 0.0 4.696758485163879E-4 8.05158597456665E-5 24-25 0.0011540606563545531 0.0 0.0 5.501917082620545E-4 8.05158597456665E-5 26-27 0.0011540606563545531 0.0 0.0 6.978041177957764E-4 8.05158597456665E-5 28-29 0.0013687696156763306 0.0 0.0 0.0011808992762697753 8.05158597456665E-5 30-31 0.0015298013351676635 0.0 0.0 0.0029119902608016053 8.05158597456665E-5 32-33 0.0015432206451252745 0.0 0.0 0.006629139119059875 9.39351697032776E-5 34-35 0.0017847682243622741 0.0 0.0 0.01176873483282492 1.0735447966088867E-4 36-37 0.002120250973302551 0.0 0.0 0.02039735113556885 1.0735447966088867E-4 38-39 0.0024825723421580508 2.683861991522217E-5 0.0 0.03652736170461737 1.0735447966088867E-4 40-41 0.0025094109620732727 2.683861991522217E-5 0.0 0.05518020254569678 1.0735447966088867E-4 42-43 0.0025899268218189395 2.683861991522217E-5 0.0 0.07407459096601318 1.2077378961849975E-4 44-45 0.002697281301479828 2.683861991522217E-5 0.0 0.09570651861768226 1.3419309957611085E-4 46-47 0.0030998606002081604 2.683861991522217E-5 0.0 0.12509480742485052 1.476124095337219E-4 48-49 0.003381666109317993 2.683861991522217E-5 0.0 0.15523457758964504 2.1470895932177735E-4 50-51 0.003381666109317993 2.683861991522217E-5 0.0 0.18858156283430855 2.1470895932177735E-4 52-53 0.003395085419275604 4.025792987283325E-5 0.0 0.2218211935993112 2.1470895932177735E-4 54-55 0.0034353433491484376 6.709654978805542E-5 0.0 0.25930132631091896 2.2812826927938841E-4 56-57 0.0036500523084702146 8.05158597456665E-5 0.0 0.3031690505623496 2.415475792369995E-4 58-59 0.003676890928385437 8.05158597456665E-5 0.0 0.3562692600646167 2.415475792369995E-4 60-61 0.003690310238343048 8.05158597456665E-5 0.0 0.4139588735723867 2.549668891946106E-4 62-63 0.0037574067881311034 8.05158597456665E-5 0.0 0.47419815597210285 2.952248190674438E-4 64-65 0.003905019197664825 1.3419309957611085E-4 0.0 0.5382887803296534 3.22063438982666E-4 66-67 0.00433443711630838 1.3419309957611085E-4 0.0 0.6075726776407995 3.22063438982666E-4 68-69 0.004374695046181213 1.3419309957611085E-4 0.0 0.6872967980989668 3.22063438982666E-4 70-71 0.004522307455714935 1.3419309957611085E-4 0.0 0.7808562271234314 3.22063438982666E-4 72-73 0.004710177795121491 1.3419309957611085E-4 0.0 0.8763882947116647 3.22063438982666E-4 74-75 0.004857790204655212 1.3419309957611085E-4 0.0 0.9766171207850618 3.22063438982666E-4 76-77 0.004924886754443268 1.3419309957611085E-4 0.0 1.0868835907067522 3.22063438982666E-4 78-79 0.004938306064400879 1.3419309957611085E-4 0.0 1.216594640757021 3.22063438982666E-4 80-81 0.005891077071391266 1.3419309957611085E-4 0.0 1.361335317959814 3.354827489402771E-4 82-83 0.006092366720755432 1.3419309957611085E-4 0.0 1.5163149286602644 4.025792987283325E-4 84-85 0.006253398440246765 1.3419309957611085E-4 0.0 1.6743004647912196 4.025792987283325E-4 86-87 0.006401010849780488 1.3419309957611085E-4 0.0 1.8479731742626224 4.159986086859436E-4 88-89 0.006427849469695709 1.3419309957611085E-4 0.0 2.0425800072678983 4.294179186435547E-4 90-91 0.006669397048932709 1.3419309957611085E-4 0.0 2.257812319678022 4.294179186435547E-4 92-93 0.006763332218635986 1.3419309957611085E-4 0.0 2.4960587486654497 4.294179186435547E-4 94-95 0.006763332218635986 1.3419309957611085E-4 0.0 2.739820514045455 4.696758485163879E-4 96-97 0.006790170838551208 1.3419309957611085E-4 0.0 2.9893794213271483 5.099337783892212E-4 98-99 0.007071976347661041 1.3419309957611085E-4 0.0 3.266112431273004 5.367723983044434E-4 100-101 0.007407459096601318 1.3419309957611085E-4 0.0 3.5765144899025056 5.367723983044434E-4 102-103 0.007716103225626374 1.3419309957611085E-4 0.0 3.912144851252317 5.636110182196655E-4 104-105 0.008588358372871092 1.3419309957611085E-4 0.0 4.2571553102624975 5.904496381348876E-4 106-107 0.00891042181185376 1.3419309957611085E-4 0.0 4.605614531931785 6.172882580501098E-4 108-109 0.00903119560147226 1.476124095337219E-4 0.0 4.974471104736641 6.44126877965332E-4 110-111 0.009245904560794037 1.8787033940655516E-4 0.0 5.3809554226626375 6.44126877965332E-4 112-113 0.009393516970327758 1.8787033940655516E-4 0.0 5.824812518820583 6.44126877965332E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9965 0.0 46.108795 1 GGTATCA 5990 0.0 44.770546 1 ATCAACG 12170 0.0 37.530025 3 TCAACGC 12270 0.0 36.9815 4 CAACGCA 12395 0.0 36.530087 5 AACGCAG 12780 0.0 35.56928 6 TATCAAC 13000 0.0 35.042736 2 GTACATG 13125 0.0 31.057283 1 ACGCAGA 15355 0.0 29.371895 7 TACATGG 13575 0.0 29.347166 2 CGCAGAG 15540 0.0 28.715925 8 ACATGGG 13535 0.0 28.421589 3 GCAGAGT 17490 0.0 24.798922 9 AGAGTAC 15505 0.0 22.850225 10-11 GAGTACT 8695 0.0 22.41004 12-13 CATGGGG 7780 0.0 21.58458 4 AGTACTT 9815 0.0 21.12581 12-13 GTACTTT 9725 0.0 20.373026 14-15 CATGGGA 7445 0.0 19.916304 4 CAGAGTA 17055 0.0 19.84911 10-11 >>END_MODULE