FastQCFastQC Report
Thu 26 May 2016
SRR1364539_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364539_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975769
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46160.1551195674126587No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33600.11291199014439629No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA119750.035.4255181
GTACATG164400.033.2595141
TACATGG162000.032.869692
ACATGGG163800.031.5641043
ATCAACG133150.031.3677623
TCAACGC133800.030.8596464
CAACGCA135900.030.5141245
TATCAAC140750.029.6317422
AACGCAG140300.029.5117786
GGTATCA82700.028.7777371
CATGGGG69850.027.5972734
ACGCAGA158750.025.7446387
CGCAGAG158800.025.2895368
CATGGGA112900.024.9788234
GAGTACT54900.023.52373912-13
GCAGAGT170400.022.5903159
TACCTGG26900.022.3386382
GTACTTT60050.021.506314-15
AGTACAT121850.020.7515722
AGTACTT59900.020.71563312-13
Produced by FastQC (version 0.11.5)