##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364539_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2975769 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.74620476253365 31.0 30.0 34.0 22.0 34.0 2 28.668283727668378 31.0 28.0 34.0 16.0 34.0 3 28.788065538689327 31.0 28.0 34.0 20.0 34.0 4 32.12582629901716 35.0 32.0 37.0 20.0 37.0 5 32.20578714275201 35.0 33.0 37.0 22.0 37.0 6 32.240292509263995 35.0 33.0 37.0 22.0 37.0 7 32.0050907849366 35.0 32.0 37.0 19.0 37.0 8 31.97201966953752 35.0 32.0 37.0 18.0 37.0 9 33.09876505871255 37.0 33.0 39.0 17.0 39.0 10-11 33.04435895393762 37.0 32.5 39.0 17.0 39.0 12-13 33.00438239661748 37.0 33.0 39.0 17.0 39.0 14-15 33.77049176196136 38.0 32.5 41.0 15.0 41.0 16-17 33.898772384550014 38.0 33.0 41.0 17.0 41.0 18-19 33.701942758325664 38.5 32.0 41.0 14.0 41.0 20-21 33.41965471782252 38.5 32.0 41.0 10.5 41.0 22-23 33.32304204392209 38.0 32.0 41.0 10.5 41.0 24-25 33.14275217599216 38.0 32.0 41.0 10.0 41.0 26-27 33.05790133575556 38.0 31.5 40.0 10.0 41.0 28-29 33.11445209624806 38.0 32.0 40.0 10.0 41.0 30-31 33.06009858292092 38.0 31.5 40.0 9.5 41.0 32-33 33.056028710561876 38.0 32.0 40.0 9.0 41.0 34-35 32.5747899114481 38.0 30.5 40.0 8.0 41.0 36-37 32.44994688767844 38.0 30.5 40.0 8.0 41.0 38-39 32.26221692611221 38.0 30.0 40.0 8.0 41.0 40-41 32.47892527948238 38.0 30.5 40.0 6.5 41.0 42-43 32.3699141297594 38.0 30.5 40.0 2.0 41.0 44-45 32.22418104362268 38.0 30.5 40.0 2.0 41.0 46-47 32.176748934477104 38.0 31.0 40.0 2.0 41.0 48-49 32.02281847146065 38.0 30.0 40.0 2.0 41.0 50-51 31.86670101073034 37.0 30.0 40.0 2.0 41.0 52-53 31.799024218613745 37.0 30.0 40.0 2.0 41.0 54-55 31.814997736719484 37.0 30.0 40.0 2.0 41.0 56-57 31.68541711403002 37.0 30.0 40.0 2.0 41.0 58-59 31.48208496694468 37.0 30.0 40.0 2.0 41.0 60-61 31.260769233095715 36.0 30.0 40.0 2.0 41.0 62-63 30.967279382236995 36.0 30.0 39.5 2.0 41.0 64-65 30.66832388535535 35.0 29.0 39.0 2.0 41.0 66-67 30.411698791136004 35.0 29.0 39.0 2.0 41.0 68-69 30.368749220789653 35.0 29.0 39.0 2.0 40.0 70-71 30.121369971929944 35.0 29.0 38.0 2.0 40.0 72-73 29.822624336768076 35.0 29.0 37.0 2.0 40.0 74-75 29.51775826685472 35.0 29.0 37.0 2.0 39.0 76-77 29.211764589254074 35.0 29.0 36.0 2.0 39.0 78-79 28.909367460982352 34.0 29.0 36.0 2.0 38.5 80-81 28.6202539578845 34.0 29.0 35.5 2.0 37.0 82-83 28.38512145936059 34.0 28.5 35.0 2.0 37.0 84-85 28.155361521677253 34.0 28.0 35.0 2.0 36.5 86-87 27.965679627686153 34.0 28.0 35.0 2.0 36.0 88-89 27.780989384592687 34.0 28.0 35.0 2.0 36.0 90-91 27.655797375401114 34.0 27.0 35.0 2.0 35.5 92-93 27.538704785216865 34.0 27.0 35.0 2.0 35.0 94-95 27.427385996695307 34.0 27.0 35.0 2.0 35.0 96-97 27.324451091465768 34.0 27.0 35.0 2.0 35.0 98-99 27.235526346299057 34.0 27.0 35.0 2.0 35.0 100-101 27.14624488661586 34.0 26.5 35.0 2.0 35.0 102-103 25.98643174251765 32.0 24.5 34.0 2.0 35.0 104-105 26.85864964652834 33.5 25.5 35.0 2.0 35.0 106-107 27.008515109875802 34.0 26.0 35.0 2.0 35.0 108-109 26.9681321029959 34.0 26.0 35.0 2.0 35.0 110-111 26.888628620030655 34.0 25.5 35.0 2.0 35.0 112-113 26.801607752483477 34.0 25.0 35.0 2.0 35.0 114-115 26.678049102601715 34.0 25.0 35.0 2.0 35.0 116-117 26.5742082130703 34.0 25.0 35.0 2.0 35.0 118-119 26.440518568477593 34.0 25.0 35.0 2.0 35.0 120-121 26.264737619082663 34.0 25.0 35.0 2.0 35.0 122-123 26.038473416451346 34.0 24.0 35.0 2.0 35.0 124-125 24.598506638116064 32.0 12.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6966779119871447 1101 2 0.7660936042112567 1101 3 0.7716981822808044 1101 4 0.7747810055487179 1101 5 0.6394977536321633 1101 6 0.7667147328243118 1101 7 0.753154383532916 1101 8 0.8205019893887666 1101 9 1.0236093794691499 1101 10-11 1.0932886749562698 1101 12-13 0.8652699847372034 1101 14-15 1.2213182668147695 1101 16-17 1.086451110690497 1101 18-19 1.4138899442711335 1101 20-21 1.746893712441178 1101 22-23 1.6371323279608987 1101 24-25 1.8704886903935432 1101 26-27 1.9315348891274766 1101 28-29 1.7133823829676658 1101 30-31 1.7698643575893556 1101 32-33 1.7360466098367482 1101 34-35 2.007995504362526 1101 36-37 2.0029734449709267 1101 38-39 1.769588033775932 1101 40-41 1.5701795346689167 1101 42-43 1.665506203083126 1101 44-45 1.6202485628739538 1101 46-47 1.4071957950863592 1101 48-49 1.3991304816265995 1101 50-51 1.3301608127178284 1101 52-53 1.2825542198392412 1101 54-55 1.1280347900213243 1101 56-57 1.096442641114983 1101 58-59 1.1693403570404293 1101 60-61 1.0521232051770468 1101 62-63 1.1308063315228836 1101 64-65 1.1575016105426421 1101 66-67 1.1411002701347925 1101 68-69 0.9571285902997531 1101 70-71 0.9701799226180405 1101 72-73 0.8008029110148485 1101 74-75 0.8470467487701683 1101 76-77 0.9627344341210211 1101 78-79 0.9345625591753937 1101 80-81 0.9607361042043827 1101 82-83 0.8169674411531425 1101 84-85 0.9480134071592943 1101 86-87 0.9411700563349541 1101 88-89 0.8961299311550128 1101 90-91 0.9088345007812322 1101 92-93 0.8084004502716589 1101 94-95 0.9058358289080424 1101 96-97 0.816288147257648 1101 98-99 0.8685701657554965 1101 100-101 0.976778623610155 1101 102-103 0.9801619865883779 1101 104-105 1.0028365456552386 1101 106-107 0.9769212849519278 1101 108-109 0.9731583037782059 1101 110-111 0.7839020990944476 1101 112-113 0.7916626239790929 1101 114-115 0.9568353210594047 1101 116-117 1.0908952992125833 1101 118-119 1.1090312017131936 1101 120-121 1.1469330694773348 1101 122-123 1.2522576346279202 1101 124-125 1.5032374669480184 1102 1 0.1512808107648027 1102 2 0.33807229754917145 1102 3 0.4603650724040058 1102 4 0.5909485547259763 1102 5 0.57815312432637 1102 6 -0.06864342049500749 1102 7 -0.6246310500777561 1102 8 0.3073442019591326 1102 9 0.5516132642691502 1102 10-11 0.5247141512846554 1102 12-13 0.6337597896347802 1102 14-15 0.865754321445003 1102 16-17 0.6986195626647742 1102 18-19 0.8143870025351845 1102 20-21 1.2422345876364247 1102 22-23 1.1156498161871156 1102 24-25 1.318375609154245 1102 26-27 0.9349733622261454 1102 28-29 1.2774623414651032 1102 30-31 1.1829979491210025 1102 32-33 1.0458258455868616 1102 34-35 1.432683822254731 1102 36-37 1.5071234212082842 1102 38-39 1.5763186730308405 1102 40-41 1.2831740061585393 1102 42-43 1.21809326295174 1102 44-45 1.2797594214139352 1102 46-47 1.0922088344683303 1102 48-49 0.6848291411116705 1102 50-51 1.0848654035874716 1102 52-53 0.8037516257453916 1102 54-55 0.7730960795000854 1102 56-57 0.6898879687836015 1102 58-59 0.8755735863319138 1102 60-61 0.7752031151501555 1102 62-63 0.6272179019635828 1102 64-65 0.7364892392875539 1102 66-67 0.6875241806363768 1102 68-69 0.6578880010106225 1102 70-71 0.6244783414795663 1102 72-73 0.7410956462104963 1102 74-75 0.3957015547065268 1102 76-77 0.5759745497883237 1102 78-79 0.48097941020389356 1102 80-81 0.155445470030255 1102 82-83 0.26477244963776414 1102 84-85 -0.006223622320451483 1102 86-87 0.44308959587585406 1102 88-89 0.5950336179227769 1102 90-91 0.1263558530113258 1102 92-93 0.4515930691924339 1102 94-95 0.3933805992197996 1102 96-97 0.6095083567399975 1102 98-99 0.7017097076329044 1102 100-101 0.6658036354507004 1102 102-103 0.5542289841990637 1102 104-105 0.7745758744334061 1102 106-107 0.730129343985233 1102 108-109 0.24442500465655925 1102 110-111 0.493824858774488 1102 112-113 0.24190893587589102 1102 114-115 0.2557857838448214 1102 116-117 0.21238972289147284 1102 118-119 0.5337785184508022 1102 120-121 0.8260493080595914 1102 122-123 0.6442109974550689 1102 124-125 1.0031799972303546 1103 1 0.6668524678458922 1103 2 0.6943636915764166 1103 3 0.49208744374902125 1103 4 0.5568904970830175 1103 5 0.5839530357866565 1103 6 0.7043702110091914 1103 7 0.711526276645607 1103 8 0.8519066993518223 1103 9 1.0744387021889281 1103 10-11 1.1678688392506658 1103 12-13 1.0384316447783277 1103 14-15 1.3551155466552842 1103 16-17 1.1990497305477774 1103 18-19 1.2693239786985728 1103 20-21 1.6826429101202223 1103 22-23 1.5724120677606948 1103 24-25 1.6777549190052525 1103 26-27 1.5082152232038553 1103 28-29 1.5289920953533525 1103 30-31 1.406230174711716 1103 32-33 1.4434325026274735 1103 34-35 1.8103271715961924 1103 36-37 1.7827903643280578 1103 38-39 1.881267463828685 1103 40-41 1.6132810414542504 1103 42-43 1.4274418921116876 1103 44-45 1.168570013054719 1103 46-47 1.2553071390760735 1103 48-49 1.2092923613296023 1103 50-51 1.2557085201853013 1103 52-53 1.2355073240105199 1103 54-55 1.0057718404777276 1103 56-57 1.0127972610325244 1103 58-59 0.9670879702991222 1103 60-61 0.7609644570435776 1103 62-63 0.9284743470879491 1103 64-65 1.0474254346577077 1103 66-67 1.068410328031959 1103 68-69 1.1134152419152201 1103 70-71 1.0855934063682184 1103 72-73 1.023334966185665 1103 74-75 1.0689187279237018 1103 76-77 1.0696629821560002 1103 78-79 0.9518859117707734 1103 80-81 0.9549898049519463 1103 82-83 1.102980292562588 1103 84-85 1.0376738154449114 1103 86-87 0.9582374671558007 1103 88-89 1.0220176982087459 1103 90-91 1.0288320385063798 1103 92-93 1.0057877625380698 1103 94-95 0.9442826053104874 1103 96-97 1.0634821055250647 1103 98-99 0.9745167776097823 1103 100-101 0.9827543981561817 1103 102-103 0.9110530485703023 1103 104-105 0.9450966958976217 1103 106-107 1.0417463206965465 1103 108-109 1.1073954030043147 1103 110-111 1.1254411319001925 1103 112-113 1.1514237793529993 1103 114-115 1.1494061302953575 1103 116-117 1.136203932083042 1103 118-119 1.2148883033341704 1103 120-121 1.2284872010635226 1103 122-123 1.299824443162855 1103 124-125 1.3968360677732186 1104 1 0.659927733869555 1104 2 0.6914065063050678 1104 3 0.5903462963611332 1104 4 0.7147771773497809 1104 5 0.6482228570467043 1104 6 0.5666227497109233 1104 7 0.5817930334547583 1104 8 0.5980352468670489 1104 9 1.020874875699434 1104 10-11 0.9337705494976802 1104 12-13 0.8725462211922945 1104 14-15 0.9713512318611919 1104 16-17 0.9376777294337799 1104 18-19 1.2414407476612155 1104 20-21 1.4726083095764153 1104 22-23 1.2061409307746231 1104 24-25 1.6875592674945281 1104 26-27 1.576329336112238 1104 28-29 1.5310545721657718 1104 30-31 1.4912791429443644 1104 32-33 1.4546944580699233 1104 34-35 1.8026796247806516 1104 36-37 1.8476323099099403 1104 38-39 1.8748906210003753 1104 40-41 1.5283030259863537 1104 42-43 1.6208497307684482 1104 44-45 1.4694813280033117 1104 46-47 1.3388794582473977 1104 48-49 1.2685027633395052 1104 50-51 1.1566729512986313 1104 52-53 1.2931017054569125 1104 54-55 1.1876018313552308 1104 56-57 1.1266777031637005 1104 58-59 1.0322911008807587 1104 60-61 1.0822091269754495 1104 62-63 1.0803950659841206 1104 64-65 1.0884023538512686 1104 66-67 1.095461758453471 1104 68-69 1.1046011814587402 1104 70-71 1.0106041083282236 1104 72-73 0.9613833616789336 1104 74-75 0.9939017770031384 1104 76-77 1.0232779320314656 1104 78-79 1.0610847999478388 1104 80-81 1.0925247769890767 1104 82-83 1.039207681904319 1104 84-85 1.0581310179378036 1104 86-87 1.0547859497408822 1104 88-89 0.9870597581416867 1104 90-91 0.9918354365631998 1104 92-93 1.0195043539711897 1104 94-95 1.0841807177307636 1104 96-97 0.9030577322170821 1104 98-99 0.9546100002922309 1104 100-101 1.0544745795433315 1104 102-103 0.9759443215770531 1104 104-105 1.0770326238781038 1104 106-107 1.0197503239676564 1104 108-109 1.0756663057773785 1104 110-111 1.1047261720854387 1104 112-113 1.1441881718900682 1104 114-115 1.0831161726209935 1104 116-117 1.1593932096539987 1104 118-119 1.1373537593754435 1104 120-121 1.1132170048658416 1104 122-123 1.1612230638647247 1104 124-125 1.438508737378374 1105 1 0.19957634815485292 1105 2 0.4450134956998859 1105 3 0.46872062405103065 1105 4 0.4785575527117132 1105 5 0.4876608831401441 1105 6 0.37889777060203755 1105 7 0.513768776359079 1105 8 0.43520870788048427 1105 9 0.34721302285635147 1105 10-11 0.5948715780184273 1105 12-13 0.5045244736843273 1105 14-15 0.6802886941419644 1105 16-17 0.5967520487020863 1105 18-19 0.714239012763926 1105 20-21 1.0561876428320431 1105 22-23 1.1770735104594579 1105 24-25 1.2977520062437975 1105 26-27 1.1718021122736673 1105 28-29 0.8497073965257584 1105 30-31 0.8647511599555102 1105 32-33 0.9529405458278006 1105 34-35 1.493405660570751 1105 36-37 1.4501556038306802 1105 38-39 1.5131869053385927 1105 40-41 1.096776209714477 1105 42-43 0.7525932161562565 1105 44-45 0.9469039669457899 1105 46-47 0.922059911840897 1105 48-49 0.8840710389655939 1105 50-51 0.8155547079714154 1105 52-53 0.8411744265521932 1105 54-55 0.6131601000003215 1105 56-57 0.6462711403022681 1105 58-59 0.6701320880946433 1105 60-61 0.677707505757656 1105 62-63 0.6360978989466872 1105 64-65 0.7501360397816761 1105 66-67 0.6255456583141665 1105 68-69 0.37801282358091726 1105 70-71 0.6479841035841396 1105 72-73 0.7059238380710724 1105 74-75 0.7348457728989928 1105 76-77 0.7388075744851932 1105 78-79 0.7886855351605178 1105 80-81 0.6999244652693299 1105 82-83 0.6617148951591894 1105 84-85 0.6381387735290573 1105 86-87 0.6165063396825516 1105 88-89 0.7991766750070077 1105 90-91 0.785070722235119 1105 92-93 0.7059938678761135 1105 94-95 0.7365855663507439 1105 96-97 0.8037618404845546 1105 98-99 0.661562233909752 1105 100-101 0.6286327516175589 1105 102-103 0.5159276038619964 1105 104-105 0.649140535184447 1105 106-107 0.6137762986830779 1105 108-109 0.6734202971680432 1105 110-111 0.7375091093985944 1105 112-113 0.7205880573177339 1105 114-115 0.6865368613242531 1105 116-117 0.7019219619280079 1105 118-119 0.7541776251684809 1105 120-121 0.698844137562947 1105 122-123 0.8136013446378811 1105 124-125 0.9038402792521687 1106 1 0.5436305451145351 1106 2 0.7497521312290054 1106 3 0.5048691149080504 1106 4 0.6958878016652221 1106 5 0.4951168572900997 1106 6 0.5992429273913444 1106 7 0.45103630958960395 1106 8 0.6548854355003684 1106 9 0.7783464590023428 1106 10-11 0.9992914208619226 1106 12-13 1.0089069367819263 1106 14-15 1.2933329770236597 1106 16-17 1.1189212489664655 1106 18-19 1.0471248172730014 1106 20-21 1.506311972713803 1106 22-23 1.447787030324335 1106 24-25 1.685595290373925 1106 26-27 1.879302075554861 1106 28-29 1.784590777593536 1106 30-31 1.5864955236185807 1106 32-33 1.4598272315882426 1106 34-35 1.7731271116980167 1106 36-37 1.7579952160800758 1106 38-39 1.9751833068954738 1106 40-41 1.656080682065216 1106 42-43 1.5520073308658056 1106 44-45 1.40910430475882 1106 46-47 1.0966883909478895 1106 48-49 1.2334274317099556 1106 50-51 1.2433197810418477 1106 52-53 1.1594917504855147 1106 54-55 0.9330512654552692 1106 56-57 1.0292524871481206 1106 58-59 1.0334356791940031 1106 60-61 1.0994130943601377 1106 62-63 0.9950257097713902 1106 64-65 0.979407465205778 1106 66-67 0.7966011037133001 1106 68-69 0.8821097612323818 1106 70-71 0.9773639963652201 1106 72-73 0.985644040758892 1106 74-75 1.040093331098305 1106 76-77 0.9009385137522834 1106 78-79 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92-93 -2.520220381268704 2216 94-95 -2.560536293309191 2216 96-97 -2.5183227319451866 2216 98-99 -2.55043608721752 2216 100-101 -2.5589538348583716 2216 102-103 -2.4099156285220573 2216 104-105 -2.630196746531386 2216 106-107 -2.545256841543303 2216 108-109 -2.6490861495307527 2216 110-111 -2.7300478642085544 2216 112-113 -2.7076884167463717 2216 114-115 -2.825104372668111 2216 116-117 -2.8367381073751474 2216 118-119 -2.885952189536134 2216 120-121 -2.875438793784383 2216 122-123 -2.9225018281545516 2216 124-125 -2.9649226523669583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 176638.0 3 29131.0 4 18721.0 5 15316.0 6 14136.0 7 14747.0 8 15336.0 9 16059.0 10 17154.0 11 18192.0 12 19341.0 13 21522.0 14 24612.0 15 21963.0 16 16667.0 17 15062.0 18 13788.0 19 13288.0 20 13256.0 21 14139.0 22 17035.0 23 22796.0 24 30276.0 25 38261.0 26 47034.0 27 53159.0 28 62021.0 29 72864.0 30 83075.0 31 96543.0 32 118873.0 33 149751.0 34 195674.0 35 270113.0 36 425404.0 37 589400.0 38 193898.0 39 524.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.157290875109354 18.544530486712592 12.985903460059802 23.31227517811825 2 14.994628008491679 20.20772820713942 40.671739224678966 24.125904559689936 3 19.300622309093736 25.79458926826842 28.604944798088138 26.299843624549702 4 12.432583574858445 16.522434949535143 37.077487887896496 33.96749358770991 5 13.698250765118011 38.56107399512933 33.42202854244766 14.318646697304995 6 31.716763365140494 36.579310391179355 17.653898435517828 14.050027808162326 7 28.710086884573204 32.83838958554651 20.52647621941035 17.92504731046994 8 26.311081270085147 35.62584999037224 19.393608845310236 18.669459894232382 9 26.871640906263455 16.754931879687778 19.40695455274086 36.96647266130791 10-11 24.92913596451875 27.290424760792924 26.94303556492456 20.837403709763763 12-13 25.809705969777834 24.482002081216507 27.281747415324347 22.426544533681312 14-15 22.835136734839985 25.59448724813131 25.24955372913047 26.32082228789824 16-17 22.67582583902448 29.338103516172225 25.483496161258895 22.5025744835444 18-19 22.551025792083152 27.84311280772334 27.662695594808852 21.943165805384655 20-21 23.340204390856133 27.02063115259175 27.601100242999177 22.03806421355294 22-23 23.11516474550116 26.900949050495832 27.35447091468224 22.62941528932077 24-25 22.845709668843725 26.95611682775861 27.53420269991863 22.663970803479035 26-27 22.753762138879623 27.25523724855703 27.29800744655196 22.692993166011387 28-29 22.6011349739948 27.083499257834408 27.71725524077787 22.59811052739292 30-31 22.74529664012238 26.964781782954265 27.511305252832212 22.77861632409114 32-33 22.68463135734843 27.152799517795895 27.21604931736763 22.946519807488045 34-35 22.985083154231482 26.839366223099013 27.39729019877365 22.778260423895855 36-37 23.03334449695398 26.68216802330144 27.332055426452268 22.952432053292313 38-39 22.811380856511377 26.817639406822234 27.591540203557468 22.77943953310892 40-41 22.928615761805705 26.80020241788874 27.49543828666131 22.775743533644246 42-43 22.9182816019127 26.812123285634033 27.277611128689973 22.991983983763294 44-45 22.865565151894796 26.969079912673372 27.09352254663574 23.071832388796096 46-47 22.96873225180078 27.154719601136424 26.693610883523423 23.182937263539376 48-49 23.12223061609837 27.14162187053842 26.55502718019262 23.18112033317059 50-51 23.0460118055335 27.30776940814551 26.594174662009518 23.05204412431147 52-53 23.324004652242834 27.130432503329395 26.49169004717772 23.053872797250055 54-55 23.044201767721155 27.102458521875356 26.563023591686463 23.290316118717026 56-57 22.97190188351932 27.50936651491711 26.460730202530456 23.058001399033113 58-59 23.04195464622327 26.965203186680654 26.554165472294315 23.438676694801767 60-61 23.212111812566068 26.88609393507045 26.814663935377947 23.087130316985537 62-63 23.098345364246757 27.068630828830493 26.746695350381096 23.08632845654165 64-65 22.896174753189555 26.917572554187718 26.79603691644996 23.390215776172763 66-67 22.886250935911722 27.420129932103553 26.49663542256162 23.1969837094231 68-69 22.750904115445785 27.241995391485897 26.74785149163293 23.259249001435393 70-71 22.732784831715076 27.273440630846874 26.707352010841213 23.28642252659684 72-73 22.93576486214346 27.167145255850283 26.4680487945871 23.429041087419154 74-75 22.791335617784846 27.467706666747315 26.380693528294703 23.36026418717313 76-77 23.05798826351785 27.108423916102787 26.605280593602586 23.228307226776774 78-79 22.994643510942588 27.245019846347283 26.604018870622447 23.156317772087682 80-81 22.900143060362602 27.587596327142787 26.493236642406558 23.01902397008805 82-83 22.969027979129745 27.38618041091259 26.396906561710487 23.24788504824718 84-85 22.934575902901067 27.276982857204306 26.388741868068387 23.39969937182624 86-87 23.07359084769766 27.06515423270795 26.67637562966271 23.184879289931683 88-89 23.142866505729298 27.005497806507123 26.611248866702347 23.24038682106123 90-91 22.921134972325497 27.302620632908713 26.777247043061443 22.99899735170435 92-93 22.96244579933846 27.08029301333414 26.58444885247086 23.372812334856537 94-95 22.88295525686651 27.06702016361138 26.61124439607904 23.43878018344307 96-97 23.06689414426678 27.05010489917109 26.465781897112095 23.417219059450034 98-99 22.93943305876302 26.99121445569598 26.40579714528199 23.663555340259006 100-101 22.83013522396848 27.10256534346404 26.73340148976242 23.33389794280506 102-103 22.989318055265713 26.803979072300304 26.76703063981109 23.439672232622897 104-105 22.977327354953292 26.95059771198423 26.855494757995746 23.216580175066728 106-107 22.83913309759583 27.073486083842234 26.745436261401473 23.341944557160463 108-109 22.985688741296787 27.01200933271366 26.38452447081746 23.617777455172092 110-111 22.703240564470033 27.120702801683073 26.783258119725907 23.39279851412099 112-113 22.826082938561427 27.084444390676833 26.67781000474163 23.41166266602011 114-115 22.809280559075653 27.17326176863863 26.585430522328853 23.43202714995687 116-117 22.609130614641124 27.06441595432979 26.78531162869161 23.541141802337478 118-119 22.74665809073218 27.241563441248296 26.390338094119535 23.62144037389999 120-121 22.533788745026918 27.197339578441742 26.68605997306915 23.5828117034622 122-123 22.815749475177675 27.219451509845015 26.48517072393724 23.479628291040065 124-125 22.84209027911877 27.183312266782693 26.31480233155541 23.659795122543127 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1387.0 1 1455.5 2 2040.0 3 2221.5 4 1581.5 5 1127.5 6 944.0 7 1084.0 8 1547.5 9 1799.0 10 1618.0 11 1456.5 12 1492.5 13 1642.0 14 1790.0 15 1979.0 16 2202.0 17 2321.5 18 2513.0 19 2780.5 20 3206.0 21 3833.0 22 4642.5 23 5766.5 24 7270.5 25 9395.5 26 12243.0 27 15864.5 28 19904.5 29 24321.0 30 29894.5 31 35372.5 32 40466.0 33 46107.0 34 52363.5 35 57571.0 36 63430.5 37 70364.5 38 76101.5 39 80032.0 40 82061.5 41 86511.5 42 91036.0 43 96059.5 44 102590.0 45 106798.5 46 108268.5 47 109898.0 48 117949.5 49 121752.5 50 119376.5 51 116747.0 52 113750.5 53 112860.5 54 109435.0 55 101037.5 56 90832.5 57 81827.0 58 75716.5 59 70513.5 60 61854.5 61 50289.0 62 38723.5 63 28880.0 64 21209.5 65 15914.5 66 12129.0 67 9670.0 68 7554.0 69 5464.0 70 3967.5 71 2980.0 72 2362.5 73 1900.0 74 1461.5 75 1107.0 76 795.5 77 536.0 78 364.0 79 248.5 80 193.0 81 136.5 82 106.0 83 89.0 84 59.5 85 51.0 86 40.0 87 36.5 88 36.5 89 27.0 90 23.5 91 20.0 92 13.5 93 12.5 94 14.0 95 9.5 96 5.0 97 5.0 98 4.5 99 4.0 100 6.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012030503711813653 2 0.005040713845731977 3 0.00799793263522807 4 0.007829908840370338 5 0.013811555937305616 6 8.065142153171163E-4 7 0.0071242089019678614 8 0.0 9 0.002621171199780628 10-11 0.0 12-13 0.003545302071498157 14-15 0.018751455506122953 16-17 0.030261085453877637 18-19 0.005578389989276722 20-21 0.008216363568543123 22-23 0.001949076020349698 24-25 0.011627246604155095 26-27 0.019037095957381102 28-29 6.048856614878373E-4 30-31 0.0016970403280630992 32-33 0.0089220635069456 34-35 0.009207703958203745 36-37 0.011106372840096123 38-39 0.0 40-41 0.024716300223572463 42-43 0.009694972963291169 44-45 0.016886391383202123 46-47 0.0038645472817278492 48-49 0.024077809803113078 50-51 0.00438542104578682 52-53 0.0 54-55 0.017676103219033467 56-57 0.004788678153445379 58-59 2.6883807177237215E-4 60-61 0.004318211527843727 62-63 0.026682178623407934 64-65 0.01713842707548872 66-67 0.0020498902972643372 68-69 0.020952567218759252 70-71 0.0029068116510387737 72-73 0.011996898952842106 74-75 0.0 76-77 0.005897635199506413 78-79 0.0010921546665752617 80-81 0.0034276854150977446 82-83 0.0017138427075488723 84-85 0.0 86-87 0.005645599507219814 88-89 0.016516739034515112 90-91 0.021775883813562144 92-93 0.004587049599616099 94-95 0.024581881187686276 96-97 0.0178441270138912 98-99 9.577356306890758E-4 100-101 0.00877084209157364 102-103 0.0 104-105 0.001562621292176913 106-107 5.040713845731977E-5 108-109 0.0 110-111 7.393046973740233E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.022632805167336578 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2975769.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.67972941642032 #Duplication Level Percentage of deduplicated Percentage of total 1 72.39546991834628 33.79400946763002 2 12.801685702164095 11.951584493021535 3 5.363806550753886 7.511431152936426 4 2.816942356257352 5.259764278869868 5 1.6825555013962858 3.9270617766644027 6 1.0702398531268347 2.9975104052778043 7 0.7425302137248511 2.4262776622134963 8 0.5363484628906361 2.0029280896518284 9 0.3759890422243491 1.5795960079114486 >10 2.0089399425809855 16.676182701009495 >50 0.13077122550232337 4.191012631837893 >100 0.07021414625274187 5.819176712441316 >500 0.0036943317865198153 1.1678686541863925 >1k 8.127529929837132E-4 0.6955959663480431 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4616 0.1551195674126587 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3360 0.11291199014439629 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.360475897154651E-5 0.0 10-11 0.0 0.0 0.0 5.040713845731977E-5 0.0 12-13 0.0 0.0 0.0 1.0081427691463955E-4 3.360475897154651E-5 14-15 0.0 0.0 0.0 1.5122141537195932E-4 3.360475897154651E-5 16-17 0.0 0.0 0.0 2.688380717723721E-4 3.360475897154651E-5 18-19 0.0 0.0 0.0 3.528499692012384E-4 3.360475897154651E-5 20-21 0.0 0.0 0.0 5.20873764058971E-4 3.360475897154651E-5 22-23 0.0 0.0 0.0 8.737237332602094E-4 3.360475897154651E-5 24-25 0.0 0.0 0.0 0.0013945974973191802 3.360475897154651E-5 26-27 0.0 0.0 0.0 0.0018986688818923782 3.360475897154651E-5 28-29 0.0 0.0 0.0 0.0026883807177237214 3.360475897154651E-5 30-31 0.0 0.0 0.0 0.004872690050874245 5.040713845731977E-5 32-33 0.0 0.0 0.0 0.008384387363400855 6.720951794309302E-5 34-35 0.0 0.0 0.0 0.013609927383476338 6.720951794309302E-5 36-37 0.0 0.0 0.0 0.023338505105739055 6.720951794309302E-5 38-39 0.0 0.0 0.0 0.039334370376195193 6.720951794309302E-5 40-41 0.0 1.6802379485773256E-5 0.0 0.059346004343751146 6.720951794309302E-5 42-43 0.0 3.360475897154651E-5 0.0 0.0793576383113071 6.720951794309302E-5 44-45 0.0 3.360475897154651E-5 0.0 0.10079747453515377 6.720951794309302E-5 46-47 0.0 3.360475897154651E-5 0.0 0.12739564126113284 6.720951794309302E-5 48-49 0.0 3.360475897154651E-5 0.0 0.16180691444799644 6.720951794309302E-5 50-51 0.0 3.360475897154651E-5 0.0 0.19752877323475043 6.720951794309302E-5 52-53 0.0 3.360475897154651E-5 0.0 0.23363708674967715 6.720951794309302E-5 54-55 0.0 3.360475897154651E-5 0.0 0.27129121917729504 6.720951794309302E-5 56-57 0.0 6.720951794309302E-5 0.0 0.31732973896831373 6.720951794309302E-5 58-59 0.0 6.720951794309302E-5 0.0 0.3732312555174814 6.720951794309302E-5 60-61 0.0 6.720951794309302E-5 0.0 0.43810524271205187 6.720951794309302E-5 62-63 0.0 6.720951794309302E-5 0.0 0.5027944037322789 6.720951794309302E-5 64-65 0.0 6.720951794309302E-5 0.0 0.5671811219217621 6.720951794309302E-5 66-67 0.0 6.720951794309302E-5 0.0 0.6442200318640325 6.720951794309302E-5 68-69 0.0 6.720951794309302E-5 0.0 0.734734450153893 6.720951794309302E-5 70-71 0.0 6.720951794309302E-5 0.0 0.8381362935093417 6.720951794309302E-5 72-73 0.0 6.720951794309302E-5 0.0 0.9475365863412113 6.720951794309302E-5 74-75 0.0 6.720951794309302E-5 0.0 1.0572561243833105 6.720951794309302E-5 76-77 0.0 6.720951794309302E-5 0.0 1.1779980233680774 6.720951794309302E-5 78-79 0.0 6.720951794309302E-5 0.0 1.3214399370381236 6.720951794309302E-5 80-81 0.0 6.720951794309302E-5 0.0 1.481314577845256 6.720951794309302E-5 82-83 0.0 6.720951794309302E-5 0.0 1.653774200887233 6.720951794309302E-5 84-85 0.0 6.720951794309302E-5 0.0 1.8299471497955655 6.720951794309302E-5 86-87 0.0 6.720951794309302E-5 0.0 2.016789609677364 6.720951794309302E-5 88-89 0.0 6.720951794309302E-5 0.0 2.229558141105711 6.720951794309302E-5 90-91 0.0 6.720951794309302E-5 0.0 2.4723525246751343 6.720951794309302E-5 92-93 0.0 6.720951794309302E-5 0.0 2.7322853353200465 6.720951794309302E-5 94-95 0.0 6.720951794309302E-5 0.0 2.9998632286309856 6.720951794309302E-5 96-97 0.0 6.720951794309302E-5 0.0 3.2694070003417606 6.720951794309302E-5 98-99 0.0 6.720951794309302E-5 0.0 3.570488838347331 6.720951794309302E-5 100-101 0.0 6.720951794309302E-5 0.0 3.9084014921857175 6.720951794309302E-5 102-103 0.0 6.720951794309302E-5 0.0 4.28254007619543 6.720951794309302E-5 104-105 0.0 6.720951794309302E-5 0.0 4.659232621886981 6.720951794309302E-5 106-107 0.0 6.720951794309302E-5 0.0 5.029926718102111 6.720951794309302E-5 108-109 0.0 6.720951794309302E-5 0.0 5.42463141460241 6.720951794309302E-5 110-111 0.0 6.720951794309302E-5 0.0 5.868751909170369 6.720951794309302E-5 112-113 0.0 6.720951794309302E-5 0.0 6.357600337929457 6.720951794309302E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 11975 0.0 35.425518 1 GTACATG 16440 0.0 33.259514 1 TACATGG 16200 0.0 32.86969 2 ACATGGG 16380 0.0 31.564104 3 ATCAACG 13315 0.0 31.367762 3 TCAACGC 13380 0.0 30.859646 4 CAACGCA 13590 0.0 30.514124 5 TATCAAC 14075 0.0 29.631742 2 AACGCAG 14030 0.0 29.511778 6 GGTATCA 8270 0.0 28.777737 1 CATGGGG 6985 0.0 27.597273 4 ACGCAGA 15875 0.0 25.744638 7 CGCAGAG 15880 0.0 25.289536 8 CATGGGA 11290 0.0 24.978823 4 GAGTACT 5490 0.0 23.523739 12-13 GCAGAGT 17040 0.0 22.590315 9 TACCTGG 2690 0.0 22.338638 2 GTACTTT 6005 0.0 21.5063 14-15 AGTACAT 12185 0.0 20.751572 2 AGTACTT 5990 0.0 20.715633 12-13 >>END_MODULE