FastQCFastQC Report
Thu 26 May 2016
SRR1364539_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364539_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2975769
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85130.28607731312477547No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73550.2471630022357246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46250.15542201024340263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA95800.038.3429681
GTATCAA138000.035.9945261
GTACATG155950.029.7867181
TACATGG154900.028.9175722
ATCAACG172050.028.4931563
TCAACGC173000.028.302264
TATCAAC175700.028.0368442
CAACGCA174650.027.934715
ACATGGG155650.027.7832413
AACGCAG176950.027.7733556
CATGGGG67350.024.4983924
GAGTACT100700.024.48987812-13
ACGCAGA202800.024.086527
AGTACTT105800.023.73112912-13
CGCAGAG203850.023.6705868
GTACTTT107900.022.91084914-15
TACCTGG24600.022.5186942
GCAGAGT216800.021.5153479
CATGGGA105300.021.4390854
TACTTTT124500.020.19058614-15
Produced by FastQC (version 0.11.5)