##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364539_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2975769 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.02063500224648 31.0 30.0 34.0 26.0 34.0 2 29.938226387868145 31.0 30.0 34.0 26.0 34.0 3 30.215260324306087 31.0 30.0 34.0 26.0 34.0 4 33.75759408744429 35.0 35.0 37.0 28.0 37.0 5 33.17032975341836 35.0 33.0 37.0 26.0 37.0 6 33.18167942471341 36.0 33.0 37.0 26.0 37.0 7 32.96549866605909 36.0 33.0 37.0 26.0 37.0 8 32.89047436141717 36.0 33.0 37.0 26.0 37.0 9 34.135011487786855 38.0 34.0 39.0 25.0 39.0 10-11 34.068584792704 38.0 34.0 39.0 25.0 39.0 12-13 33.946711925556045 38.0 34.0 39.0 24.5 39.0 14-15 34.911893530714245 39.0 34.0 41.0 24.5 41.0 16-17 34.911516149270994 39.0 34.0 41.0 24.0 41.0 18-19 34.917998675300396 39.0 34.0 41.0 24.0 41.0 20-21 34.928048682542226 39.0 34.0 41.0 24.0 41.0 22-23 34.88687445161234 39.0 34.0 41.0 24.0 41.0 24-25 34.840820809679784 39.0 34.0 41.0 24.0 41.0 26-27 34.72241192108662 39.0 34.0 41.0 24.0 41.0 28-29 34.57149378866438 39.0 33.5 41.0 23.0 41.0 30-31 34.42017206308688 38.5 33.0 41.0 22.5 41.0 32-33 34.23102280452549 38.0 33.0 40.5 21.5 41.0 34-35 34.059995080263285 38.0 33.0 40.0 20.0 41.0 36-37 33.8644543645693 38.0 33.0 40.0 18.5 41.0 38-39 33.70470137298964 38.0 33.0 40.0 17.0 41.0 40-41 33.73879289689489 38.0 33.0 40.0 16.5 41.0 42-43 33.61840401590312 38.0 33.0 40.0 15.5 41.0 44-45 33.470482923909756 38.0 33.0 40.0 15.0 41.0 46-47 33.23580896232201 38.0 32.0 40.0 10.5 41.0 48-49 32.9665083210424 38.0 32.0 40.0 8.0 41.0 50-51 32.800096546472524 38.0 32.0 40.0 7.0 41.0 52-53 32.610047184442074 38.0 31.0 40.0 3.5 41.0 54-55 32.41339935996376 37.5 31.0 40.0 2.0 41.0 56-57 32.1968509652463 37.0 31.0 40.0 2.0 41.0 58-59 31.978873696177356 37.0 31.0 40.0 2.0 41.0 60-61 31.730821175971656 36.0 30.5 40.0 2.0 41.0 62-63 31.535226692663308 36.0 30.0 39.5 2.0 41.0 64-65 31.384352078403936 36.0 30.0 39.0 2.0 41.0 66-67 31.073312814267503 35.0 30.0 39.0 2.0 41.0 68-69 30.78453855121147 35.0 30.0 39.0 2.0 40.5 70-71 30.462600927692975 35.0 30.0 38.0 2.0 40.0 72-73 30.159791301004883 35.0 29.0 37.0 2.0 39.5 74-75 29.80565376546365 35.0 29.0 37.0 2.0 39.0 76-77 29.49269348528061 35.0 29.0 36.0 2.0 39.0 78-79 29.21074686912862 34.0 29.0 36.0 2.0 38.5 80-81 28.943171664198395 34.0 29.0 35.5 2.0 37.0 82-83 28.670168114527705 34.0 29.0 35.0 2.0 37.0 84-85 28.457557693490322 34.0 29.0 35.0 2.0 36.0 86-87 28.252414417920207 34.0 28.5 35.0 2.0 36.0 88-89 28.08329561199139 34.0 28.0 35.0 2.0 36.0 90-91 27.952055418280114 34.0 28.0 35.0 2.0 35.5 92-93 27.84852570881678 34.0 27.5 35.0 2.0 35.0 94-95 27.7472977573192 34.0 27.0 35.0 2.0 35.0 96-97 27.635315442831754 34.0 27.0 35.0 2.0 35.0 98-99 27.554240769360796 34.0 27.0 35.0 2.0 35.0 100-101 27.455551657403515 34.0 27.0 35.0 2.0 35.0 102-103 26.18662150859156 31.5 24.5 34.0 2.0 35.0 104-105 27.127313309601654 33.5 25.5 35.0 2.0 35.0 106-107 27.29029555049468 34.0 27.0 35.0 2.0 35.0 108-109 27.296263419640436 34.0 27.0 35.0 2.0 35.0 110-111 27.219249209196008 34.0 27.0 35.0 2.0 35.0 112-113 27.13555823721532 34.0 26.5 35.0 2.0 35.0 114-115 27.030906632873723 34.0 26.0 35.0 2.0 35.0 116-117 26.910689640224092 34.0 25.0 35.0 2.0 35.0 118-119 26.780461789876835 34.0 25.0 35.0 2.0 35.0 120-121 26.58170661096342 34.0 25.0 35.0 2.0 35.0 122-123 26.290170876838893 34.0 25.0 35.0 2.0 35.0 124-125 24.475699390644905 31.5 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.606127091192068 1101 2 0.838765290380838 1101 3 0.6544406392816065 1101 4 0.5756532980558973 1101 5 0.5595837947765432 1101 6 0.5702550425117465 1101 7 0.7306682307843246 1101 8 0.8438558204308393 1101 9 0.9688390126610926 1101 10-11 0.9038079047611234 1101 12-13 0.8053713823870794 1101 14-15 0.9555354091603121 1101 16-17 0.9982748145119942 1101 18-19 0.9904644094903503 1101 20-21 1.046817451658164 1101 22-23 1.008861526376748 1101 24-25 1.0458842510198068 1101 26-27 1.0512255882701282 1101 28-29 1.137351487154156 1101 30-31 1.1885453702701838 1101 32-33 1.223083664794352 1101 34-35 1.1514889648712128 1101 36-37 1.2024264243010805 1101 38-39 1.138644975943123 1101 40-41 0.9322343466817173 1101 42-43 1.0170320234626047 1101 44-45 0.9741903144720112 1101 46-47 0.955310662308456 1101 48-49 1.137975697271706 1101 50-51 1.1331689240497127 1101 52-53 1.1381013518358145 1101 54-55 1.1564910168438374 1101 56-57 1.0758245446286665 1101 58-59 1.1148733444917909 1101 60-61 1.029114136589854 1101 62-63 1.12535952094618 1101 64-65 1.057709341499276 1101 66-67 1.1481623601062374 1101 68-69 1.2061546989367429 1101 70-71 1.193793328382995 1101 72-73 1.1326710460967107 1101 74-75 1.1196311499674927 1101 76-77 1.1416185185124057 1101 78-79 1.1036207492434755 1101 80-81 0.9688268870734866 1101 82-83 1.0623105398920742 1101 84-85 1.0892321427026026 1101 86-87 1.0649978017627895 1101 88-89 1.072118102346952 1101 90-91 1.0607974784389214 1101 92-93 1.0281080044481108 1101 94-95 1.025803068181549 1101 96-97 1.0245424457126084 1101 98-99 1.0290151621537866 1101 100-101 0.9069234782805431 1101 102-103 0.7524545803787142 1101 104-105 0.9096138962882527 1101 106-107 0.8964513311041777 1101 108-109 0.941079611963886 1101 110-111 0.8262206359159272 1101 112-113 0.8229145339888895 1101 114-115 0.8569059213785906 1101 116-117 0.8758444566404009 1101 118-119 0.9216204865878552 1101 120-121 0.9952375624855101 1101 122-123 0.9154825775127406 1101 124-125 1.0989918061201074 1102 1 0.32338342009888166 1102 2 -0.4330500105606774 1102 3 0.20770821196665779 1102 4 0.3156286933710959 1102 5 0.4342254946609643 1102 6 0.3393575688958279 1102 7 0.42130208889391696 1102 8 0.39017039829881384 1102 9 0.8503656102071062 1102 10-11 0.7983319861784111 1102 12-13 0.8950437657487882 1102 14-15 1.036243892781421 1102 16-17 1.0968021217771664 1102 18-19 1.1041740948019836 1102 20-21 0.8671735110918988 1102 22-23 1.0211878505962773 1102 24-25 1.033819229246049 1102 26-27 1.097482721905898 1102 28-29 0.8035482018945643 1102 30-31 1.0044457808307854 1102 32-33 1.0414500996656457 1102 34-35 1.1715385820082105 1102 36-37 0.936110809684493 1102 38-39 0.37539567021809717 1102 40-41 0.02256573312961052 1102 42-43 0.29439535665117944 1102 44-45 0.014109646867069614 1102 46-47 0.18636826108450322 1102 48-49 -0.0844835142651732 1102 50-51 -0.19580876536487324 1102 52-53 -0.44593469215168824 1102 54-55 -0.38168936599674197 1102 56-57 -0.5028550413324737 1102 58-59 -0.31633815589446357 1102 60-61 -0.3905960294795321 1102 62-63 -0.3006618650266617 1102 64-65 -0.30515955292301555 1102 66-67 -0.49799411614732847 1102 68-69 -0.28797666169946723 1102 70-71 -0.6762467164143331 1102 72-73 -0.49188407967200476 1102 74-75 -0.4254178962951478 1102 76-77 -0.648581969503617 1102 78-79 -0.5157295060291069 1102 80-81 -0.6322485076912265 1102 82-83 -0.8226931921990364 1102 84-85 -0.7003802172981075 1102 86-87 -0.439772974852648 1102 88-89 -0.5708710916131139 1102 90-91 -0.5337651828713135 1102 92-93 -0.3235049806600223 1102 94-95 -0.18505495699686492 1102 96-97 -0.46611231557321986 1102 98-99 -0.6368787343226323 1102 100-101 -0.48429763624154276 1102 102-103 -0.43832936161881975 1102 104-105 -0.37164443108657963 1102 106-107 -0.30064288783469806 1102 108-109 -0.31801515562602845 1102 110-111 -0.25015277055456764 1102 112-113 -0.002952552800525865 1102 114-115 -0.0837228651239812 1102 116-117 -0.15045977718810732 1102 118-119 0.1302367850099806 1102 120-121 0.1934481466184259 1102 122-123 0.1585578715589051 1102 124-125 0.3556850276191561 1103 1 0.2853360943157064 1103 2 -0.15090346426318746 1103 3 0.20232593771645924 1103 4 0.3018296984400237 1103 5 0.2741482496780563 1103 6 0.3995149167890233 1103 7 0.39514660519125755 1103 8 0.3666558511993614 1103 9 0.6343607809947258 1103 10-11 0.5034643163107333 1103 12-13 0.5358415975843513 1103 14-15 0.6312747260198037 1103 16-17 0.46592811590316074 1103 18-19 0.6299909390150518 1103 20-21 0.6685651473891951 1103 22-23 0.830093532143465 1103 24-25 0.6430312040240622 1103 26-27 0.6212895994863601 1103 28-29 0.7386766747588851 1103 30-31 0.6586137628289634 1103 32-33 0.7041694487057484 1103 34-35 0.6992242865807015 1103 36-37 0.726420816975633 1103 38-39 0.7108835009889418 1103 40-41 0.5831285767113243 1103 42-43 0.6805971555888277 1103 44-45 0.6454991225961706 1103 46-47 0.6442652779437807 1103 48-49 0.5047112164389915 1103 50-51 0.4074691185733883 1103 52-53 0.40628174261192385 1103 54-55 0.46697069256897095 1103 56-57 0.5028437686116192 1103 58-59 0.6084637972093745 1103 60-61 0.5527145058121334 1103 62-63 0.46333489661160954 1103 64-65 0.45723227078956086 1103 66-67 0.5046076718318204 1103 68-69 0.5035545803034722 1103 70-71 0.43359669531587386 1103 72-73 0.37852066573274 1103 74-75 0.4364396849020551 1103 76-77 0.4563259915364206 1103 78-79 0.43722419928308653 1103 80-81 0.4067488143249598 1103 82-83 0.48140320151999916 1103 84-85 0.5282250207410932 1103 86-87 0.6220090798539353 1103 88-89 0.6106992927874586 1103 90-91 0.620252197561161 1103 92-93 0.5564861186707013 1103 94-95 0.46059481365290367 1103 96-97 0.5148472880278909 1103 98-99 0.5618510445958265 1103 100-101 0.4363189164504817 1103 102-103 0.25206144905501304 1103 104-105 0.3407359204447147 1103 106-107 0.4469282630383411 1103 108-109 0.5600196799559782 1103 110-111 0.38694594437433594 1103 112-113 0.2584537840775063 1103 114-115 0.40806899930939267 1103 116-117 0.47237584093053187 1103 118-119 0.32271048927613677 1103 120-121 0.27375940853346137 1103 122-123 0.2529938480703535 1103 124-125 0.5582579094373195 1104 1 0.23066797316656817 1104 2 0.41476248303494856 1104 3 0.1661708788630598 1104 4 -0.159968093069061 1104 5 0.2668265344909173 1104 6 0.29706625913463824 1104 7 0.3295277465171651 1104 8 0.29346236665102765 1104 9 0.27681602219968937 1104 10-11 0.31297378274791754 1104 12-13 0.3096669406129209 1104 14-15 0.4254712868280919 1104 16-17 0.3098349119183865 1104 18-19 0.2910101936862688 1104 20-21 0.2167594438103535 1104 22-23 0.41865719625839404 1104 24-25 0.5025054401009328 1104 26-27 0.3767937036265181 1104 28-29 0.5352970203903666 1104 30-31 0.3989203210884611 1104 32-33 0.41602808147253967 1104 34-35 0.565014204399489 1104 36-37 0.5845878147880015 1104 38-39 0.5854316814589282 1104 40-41 0.574839010704089 1104 42-43 0.5805072837269663 1104 44-45 0.5596599068751615 1104 46-47 0.4993373427282819 1104 48-49 0.5271246142104289 1104 50-51 0.5364901524762189 1104 52-53 0.6332903159889867 1104 54-55 0.2785914219948893 1104 56-57 0.39025100599044293 1104 58-59 0.5506651436389589 1104 60-61 0.5871802049232002 1104 62-63 0.5898803580456793 1104 64-65 0.5811586550819428 1104 66-67 0.5553200287330853 1104 68-69 0.312498343594811 1104 70-71 -0.13215122653570788 1104 72-73 -0.04573870943024971 1104 74-75 0.35173618085892855 1104 76-77 0.48131029532840586 1104 78-79 0.49182670634638015 1104 80-81 0.4401063562299008 1104 82-83 0.4954994296080457 1104 84-85 0.48407311293835775 1104 86-87 0.4659442176115327 1104 88-89 0.39117562103600534 1104 90-91 0.38933430890287823 1104 92-93 0.4149735602281588 1104 94-95 0.3649929255811344 1104 96-97 0.39349335969510335 1104 98-99 0.4486862560616345 1104 100-101 0.34075518640164404 1104 102-103 0.2724566539864739 1104 104-105 0.32380003182776207 1104 106-107 0.4229727743351823 1104 108-109 0.34916641637482115 1104 110-111 0.32823044035876237 1104 112-113 0.32155313074130376 1104 114-115 0.30443008688438766 1104 116-117 0.3341508507960178 1104 118-119 0.3483673682008046 1104 120-121 0.3354543874198477 1104 122-123 0.30956854184668714 1104 124-125 0.6851308016432434 1105 1 0.07926029957126701 1105 2 -0.10074220832557756 1105 3 0.10671179636349848 1105 4 0.09419107801411286 1105 5 -0.19282501408154928 1105 6 0.15511389159200917 1105 7 0.1730734850803799 1105 8 0.24918590371220262 1105 9 0.4476082338872729 1105 10-11 0.4434802103087847 1105 12-13 0.4354912496860237 1105 14-15 0.5226861029513259 1105 16-17 0.5272419672864146 1105 18-19 0.4373519266579322 1105 20-21 0.45218304653104013 1105 22-23 0.5817785394090436 1105 24-25 0.503699883198081 1105 26-27 0.4957770455141599 1105 28-29 0.41226419329395725 1105 30-31 0.5388463413384201 1105 32-33 0.6479705964703442 1105 34-35 0.605938944035799 1105 36-37 0.6734049954254715 1105 38-39 0.7036076841538375 1105 40-41 0.46574814987448576 1105 42-43 0.5286842492348498 1105 44-45 0.6437756645040125 1105 46-47 0.6162283158619388 1105 48-49 0.5739598189963999 1105 50-51 0.46556512568515984 1105 52-53 0.44356353045955643 1105 54-55 0.42752734474012755 1105 56-57 0.4905300769700247 1105 58-59 0.18606261286453218 1105 60-61 0.26317317518193306 1105 62-63 0.44902755188021004 1105 64-65 0.32442356112804305 1105 66-67 0.42214957729445146 1105 68-69 0.4136407368776105 1105 70-71 0.44762337870493596 1105 72-73 0.5141822337307254 1105 74-75 0.48661170410193577 1105 76-77 0.4389897905731033 1105 78-79 0.42417340974954953 1105 80-81 0.4913637923916596 1105 82-83 0.37770874514936637 1105 84-85 0.4045775545466448 1105 86-87 0.5659941680592375 1105 88-89 0.40790146812441463 1105 90-91 0.21603721498131634 1105 92-93 0.33192802110476904 1105 94-95 0.269194096062499 1105 96-97 0.2999490327749754 1105 98-99 0.20154408526343914 1105 100-101 0.29393030953432486 1105 102-103 0.03601167319808951 1105 104-105 0.14376517751536255 1105 106-107 0.11408031571864186 1105 108-109 0.15821403507771592 1105 110-111 0.051909269114876366 1105 112-113 0.1316729466630413 1105 114-115 0.09923092093621477 1105 116-117 -0.11717108659604136 1105 118-119 0.024577996932158896 1105 120-121 0.013561106189413863 1105 122-123 0.05630599336519282 1105 124-125 0.42994557114606025 1106 1 -0.12490948819402803 1106 2 -0.5406129581236243 1106 3 0.016234892493336872 1106 4 -0.12150782776542712 1106 5 0.16084965728512657 1106 6 0.23277637231463189 1106 7 0.3438911394829631 1106 8 0.4347007748291887 1106 9 0.36727482711632575 1106 10-11 0.36906749091391333 1106 12-13 0.5069539066589286 1106 14-15 0.49828131481884697 1106 16-17 0.4148963698714141 1106 18-19 0.5563685969964851 1106 20-21 0.5066415175598991 1106 22-23 0.4503837387921621 1106 24-25 0.4249063163331357 1106 26-27 0.6084411418643185 1106 28-29 0.6636047969511552 1106 30-31 0.6437671021521112 1106 32-33 0.5670209012364467 1106 34-35 0.6627127561823869 1106 36-37 0.7026106941843722 1106 38-39 0.5827655015879287 1106 40-41 0.5764148899787713 1106 42-43 0.39104908730491417 1106 44-45 0.4363829201403391 1106 46-47 0.4348196355358809 1106 48-49 0.5753940556123993 1106 50-51 0.607029765473655 1106 52-53 0.4414737162567235 1106 54-55 0.47604706273968844 1106 56-57 0.002343618866184727 1106 58-59 0.554314650758343 1106 60-61 0.46347581159230344 1106 62-63 0.4028631984984017 1106 64-65 0.49492499316152916 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-1.2981937134999484 2216 68-69 -1.2121128153729899 2216 70-71 -1.3497219299251348 2216 72-73 -1.2044949248451289 2216 74-75 -1.2332974292636472 2216 76-77 -1.3456414023281766 2216 78-79 -1.3228757571255372 2216 80-81 -1.2025514961436805 2216 82-83 -1.0756567360878435 2216 84-85 -1.086950266679544 2216 86-87 -1.2182497132937975 2216 88-89 -1.1166214683243467 2216 90-91 -1.184075019124208 2216 92-93 -1.2117088839884786 2216 94-95 -1.0985406436142249 2216 96-97 -1.2831714360131237 2216 98-99 -1.2630901496799574 2216 100-101 -1.3119595439746234 2216 102-103 -1.234905502501011 2216 104-105 -1.1084067203399712 2216 106-107 -1.2418233236770568 2216 108-109 -1.2911839855564224 2216 110-111 -1.2790645868329342 2216 112-113 -1.237964049377858 2216 114-115 -1.3046110815638023 2216 116-117 -1.3801153155625023 2216 118-119 -1.372256332251819 2216 120-121 -1.3967802558875597 2216 122-123 -1.3275870469703541 2216 124-125 -1.728841121853609 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 97840.0 3 33112.0 4 15753.0 5 12339.0 6 13146.0 7 15345.0 8 17222.0 9 18450.0 10 19966.0 11 21607.0 12 23270.0 13 26179.0 14 30048.0 15 26533.0 16 18897.0 17 16729.0 18 15697.0 19 15009.0 20 14664.0 21 15371.0 22 17420.0 23 21597.0 24 28303.0 25 37484.0 26 46216.0 27 51386.0 28 60607.0 29 70528.0 30 81616.0 31 96343.0 32 117767.0 33 145467.0 34 188924.0 35 260947.0 36 423598.0 37 644286.0 38 215598.0 39 505.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.69232735747001 19.422822828479628 14.03647276954624 23.848377044504126 2 15.373202691472358 20.893019585861673 38.898550257093206 24.835227465572764 3 19.149739109453726 25.42720889961553 29.462770799749578 25.960281191181174 4 13.001412809472551 17.089242465016145 36.07460979547901 33.8347349300323 5 13.466300643631948 38.49626768744482 33.64007757322561 14.397354095697617 6 31.786976744498645 36.92897533377087 17.0795179330116 14.204529988718884 7 28.259920576911252 32.78879083942684 20.59993097443332 18.351357609228593 8 26.295489236770877 35.68665617688435 19.12559307486169 18.892261511483085 9 26.46742333964454 16.216715765050957 19.383343112682976 37.932517782621524 10-11 25.088885155002938 27.039048979248932 26.740872049063263 21.13119381668487 12-13 25.943008345069796 24.16271222665469 27.49119639326843 22.403083035007086 14-15 22.986069980265594 25.176630902783902 25.502513217178425 26.334785899772072 16-17 22.491684482831626 28.88644992442272 25.96267779479676 22.659187797948896 18-19 22.46501005473648 27.047106709251434 28.518435251798564 21.969447984213524 20-21 23.164525398650213 26.262909021621255 28.53924198070456 22.033323599023973 22-23 22.90184486766278 26.11603924901429 28.363575936169777 22.618539947153156 24-25 22.667922140461844 26.272973473411408 28.427458582974687 22.631645803152058 26-27 22.560857378378497 26.557152117654294 28.318310325835107 22.563680178132106 28-29 22.44066995791676 26.34853377395893 28.710612282068936 22.50018398605537 30-31 22.560588540306725 26.20631843399135 28.579956979187564 22.653136046514362 32-33 22.459018156315224 26.37481269547468 28.328324544008627 22.837844604201468 34-35 22.746271636004003 26.249097292162126 28.25162168165607 22.7530093901778 36-37 22.869113832424492 26.105806599907456 28.18496664223601 22.84011292543205 38-39 22.694167457218622 26.241183371424327 28.397382323695155 22.6672668476619 40-41 22.65513552967317 26.099472102841315 28.519972484423356 22.72541988306216 42-43 22.734392353707563 26.165471849461436 28.239557573185287 22.86057822364572 44-45 22.760385634772053 26.297790587911894 27.96787989927982 22.97394387803623 46-47 22.835122618724775 26.522757646846916 27.5390663724234 23.103053362004914 48-49 23.033810756144042 26.664284089255585 27.203926111200165 23.097979043400212 50-51 22.968919966569988 26.78048934577919 27.238471803422915 23.01211888422791 52-53 23.217107913954344 26.644860538570033 27.003171281104144 23.134860266371483 54-55 22.89576240628893 26.638391622468006 27.167750588167294 23.298095383075772 56-57 22.85827686861217 27.07293707794291 27.015132259608833 23.05365379383609 58-59 22.969947836548357 26.545962898335457 27.11059508677246 23.373494178343723 60-61 23.056141118480635 26.482919205086148 27.39501957309186 23.065920103341355 62-63 22.979959129892137 26.589143848195206 27.361818071227972 23.06907895068468 64-65 22.775684290771093 26.481825588626247 27.396515213658613 23.34597490694405 66-67 22.720563995390773 26.901197640004987 27.167212912023746 23.211025452580493 68-69 22.609205421928053 26.76342712200682 27.275916670923273 23.351450785141854 70-71 22.553111481435558 26.785278023932634 27.26360816313363 23.398002331498176 72-73 22.805651245106727 26.669761664967943 26.994702881843313 23.52988420808201 74-75 22.636681708881145 27.002189205932716 26.848109703261695 23.51301938192444 76-77 22.858528333348456 26.686480032556293 27.103111834285524 23.35187979980973 78-79 22.762116279859086 26.766660987462398 27.10635469352628 23.36486803915223 80-81 22.627083159708686 27.06069491976525 27.022486295974684 23.289735624551376 82-83 22.697628747392688 26.883471129647496 26.876078082673754 23.542822040286058 84-85 22.654043375006594 26.80515523886431 26.879690594263195 23.661110791865898 86-87 22.819496405803005 26.550414363480503 27.157114681952798 23.4729745487637 88-89 22.86683507346598 26.507251495514883 27.084437403784143 23.54147602723499 90-91 22.616758894927663 26.822058432626996 27.218829821804047 23.342352850641294 92-93 22.630139039460268 26.577980590798404 27.07631678172075 23.715563588020576 94-95 22.536598529085847 26.552367900930467 27.03147103008853 23.879562539895158 96-97 22.66798184847459 26.54300148798146 27.005594843904777 23.78342181963917 98-99 22.5519201076335 26.405897057570716 26.924152963374265 24.11802987142152 100-101 22.41708933989716 26.526454421022528 27.249409635302985 23.807046603777327 102-103 22.569409789536753 26.27630034454959 27.205740768184626 23.948549097729025 104-105 22.546222505846387 26.33411733236014 27.285652885018962 23.834007276774507 106-107 22.377721313442144 26.4688615170162 27.232003785298563 23.92141338424309 108-109 22.465020380449623 26.38733490189173 26.90125256719399 24.24639215046466 110-111 22.249365582983994 26.42189139022641 27.24055523901681 24.088187787772785 112-113 22.283248464514553 26.40178051454935 27.148444654138142 24.16652636679796 114-115 22.166875184196087 26.438006444720674 27.06596177324248 24.329156597840758 116-117 21.967330125423043 26.29051515759456 27.28511520887542 24.45703950810698 118-119 22.04788073267784 26.43683027815667 26.933995884761213 24.581293104404274 120-121 21.797483515013756 26.391402737741025 27.175729180458763 24.63538456678646 122-123 21.9830876576868 26.363493537099192 27.000903167808577 24.652515637405433 124-125 21.807024730570905 26.2957551884692 26.94662606564236 24.950594015317538 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 800.0 1 567.5 2 575.5 3 839.5 4 626.5 5 466.0 6 867.0 7 2003.5 8 3357.5 9 3886.5 10 3096.0 11 1981.0 12 1681.5 13 1785.0 14 1988.0 15 2251.0 16 2561.0 17 2914.5 18 3297.0 19 3690.0 20 4145.0 21 4729.0 22 5468.0 23 6467.5 24 7920.5 25 9937.5 26 12727.5 27 16154.5 28 19976.5 29 24397.0 30 29966.0 31 35427.5 32 40263.0 33 45422.0 34 51358.5 35 56603.0 36 62420.5 37 69415.0 38 75134.0 39 78905.0 40 81472.0 41 86095.5 42 90591.5 43 95691.5 44 101935.0 45 105954.0 46 107825.0 47 109530.5 48 118215.0 49 122601.5 50 119836.0 51 117408.0 52 114492.5 53 113929.0 54 110772.5 55 102003.5 56 91135.5 57 81774.5 58 75947.0 59 70798.5 60 62152.5 61 50212.0 62 38006.5 63 28145.5 64 20664.0 65 15437.5 66 11687.5 67 9415.5 68 7189.5 69 4888.5 70 3507.0 71 2690.5 72 2137.5 73 1782.5 74 1392.0 75 1057.5 76 708.5 77 380.0 78 239.0 79 146.5 80 83.0 81 60.5 82 42.5 83 26.5 84 15.0 85 7.5 86 4.0 87 2.5 88 2.5 89 0.5 90 1.0 91 1.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09815950095588737 2 0.0 3 0.0 4 0.0997725293865216 5 0.0 6 0.0 7 0.002016285538292791 8 0.00588083282002064 9 0.003730128245841663 10-11 6.384904204593838E-4 12-13 0.0 14-15 5.040713845731977E-5 16-17 2.3523331280082558E-4 18-19 0.0019154712613781515 20-21 0.0013441903588618605 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.008014735014713843 58-59 8.905261127459827E-4 60-61 0.0 62-63 0.0 64-65 1.8482617434350583E-4 66-67 0.0 68-69 3.360475897154652E-5 70-71 0.0 72-73 0.0 74-75 0.0010921546665752617 76-77 0.0 78-79 0.0 80-81 3.360475897154652E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0012769808409187676 90-91 0.0 92-93 0.0022515188510936163 94-95 1.680237948577326E-5 96-97 0.008787644471059414 98-99 0.003881349661213622 100-101 0.00221791409212207 102-103 0.0 104-105 0.0 106-107 0.0015458189126911397 108-109 1.680237948577326E-5 110-111 1.8482617434350583E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.001041747528117942 122-123 0.004419025804758367 124-125 0.08216363568543122 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2975769.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.20634754773267 #Duplication Level Percentage of deduplicated Percentage of total 1 71.44258835281187 31.582158904339984 2 12.864704173136959 11.374031675529185 3 5.536567064865987 7.3425422367238795 4 3.0025923498538734 5.309345638472145 5 1.7587732926121475 3.887447171544135 6 1.1514854359903821 3.0541779227725985 7 0.7817596567188645 2.4191117358597216 8 0.5804535217727864 2.0527784095034565 9 0.43486192480945024 1.7301291645062291 >10 2.204252443384301 17.36693726260486 >50 0.15258472297890513 4.629331169421748 >100 0.08380383013453209 6.676710649747189 >500 0.00442804649235788 1.3382397011458558 >1k 9.924931792907375E-4 0.7015194801612918 >5k 1.5269125835242115E-4 0.535538877667631 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8513 0.28607731312477547 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7355 0.2471630022357246 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4625 0.15542201024340263 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 3.360475897154651E-5 0.0 10-11 0.0 0.0 0.0 5.040713845731977E-5 0.0 12-13 0.0 0.0 0.0 1.0081427691463955E-4 0.0 14-15 0.0 0.0 0.0 1.5122141537195932E-4 0.0 16-17 0.0 0.0 0.0 2.8564045125814536E-4 0.0 18-19 0.0 0.0 0.0 3.696523486870117E-4 0.0 20-21 0.0 0.0 0.0 5.88083282002064E-4 0.0 22-23 0.0 0.0 0.0 8.737237332602094E-4 0.0 24-25 0.0 0.0 0.0 0.0013441903588618607 0.0 26-27 0.0 0.0 0.0 0.0018146569844635118 0.0 28-29 0.0 0.0 0.0 0.002553961681837535 0.0 30-31 0.0 0.0 0.0 0.004654259117559192 0.0 32-33 0.0 0.0 0.0 0.008300375465971989 0.0 34-35 0.0 0.0 0.0 0.013542717865533245 0.0 36-37 0.0 0.0 0.0 0.02320408606985287 0.0 38-39 0.0 0.0 0.0 0.03930076561722365 0.0 40-41 0.0 0.0 0.0 0.05899315437454991 0.0 42-43 0.0 0.0 0.0 0.07843350743958957 0.0 44-45 0.0 0.0 0.0 0.09906682944811912 0.0 46-47 0.0 0.0 0.0 0.12479127244083799 0.0 48-49 0.0 0.0 0.0 0.15869847424312844 0.0 50-51 0.0 0.0 0.0 0.1934625973991933 0.0 52-53 0.0 0.0 0.0 0.22852916338600207 0.0 54-55 0.0 0.0 0.0 0.2648391054547581 0.0 56-57 0.0 0.0 0.0 0.3094494229894861 0.0 58-59 0.0 0.0 0.0 0.36375471348750527 0.0 60-61 0.0 0.0 0.0 0.4267468341796692 0.0 62-63 0.0 0.0 0.0 0.49004139770257704 0.0 64-65 0.0 0.0 0.0 0.5523782255947958 0.0 66-67 0.0 0.0 0.0 0.6277873047269462 0.0 68-69 0.0 0.0 0.0 0.7160502041657131 0.0 70-71 0.0 0.0 0.0 0.8164276192137225 0.0 72-73 0.0 0.0 0.0 0.9227698789791815 0.0 74-75 0.0 0.0 0.0 1.0288096959138966 0.0 76-77 0.0 0.0 0.0 1.1468464118014536 0.0 78-79 0.0 0.0 0.0 1.2870118614717743 0.0 80-81 0.0 0.0 0.0 1.4435428287612377 0.0 82-83 0.0 0.0 0.0 1.6120202878650862 0.0 84-85 0.0 0.0 0.0 1.783286941963573 0.0 86-87 0.0 0.0 0.0 1.965290316553469 0.0 88-89 0.0 0.0 0.0 2.1725812722694537 0.0 90-91 0.0 0.0 0.0 2.409377206362456 0.0 92-93 0.0 3.360475897154651E-5 0.0 2.663916453192435 0.0 94-95 0.0 3.360475897154651E-5 0.0 2.925395082750039 0.0 96-97 0.0 3.360475897154651E-5 0.0 3.1899653501330247 0.0 98-99 0.0 3.360475897154651E-5 0.0 3.485166355318575 0.0 100-101 0.0 3.360475897154651E-5 0.0 3.8164924763985377 0.0 102-103 0.0 3.360475897154651E-5 0.0 4.184380575239543 0.0 104-105 0.0 3.360475897154651E-5 0.0 4.554906647659815 0.0 106-107 0.0 3.360475897154651E-5 0.0 4.919148630152407 0.0 108-109 0.0 3.360475897154651E-5 0.0 5.308190924766002 0.0 110-111 0.0 3.360475897154651E-5 0.0 5.745288024708907 0.0 112-113 0.0 3.360475897154651E-5 0.0 6.226961837427569 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9580 0.0 38.342968 1 GTATCAA 13800 0.0 35.994526 1 GTACATG 15595 0.0 29.786718 1 TACATGG 15490 0.0 28.917572 2 ATCAACG 17205 0.0 28.493156 3 TCAACGC 17300 0.0 28.30226 4 TATCAAC 17570 0.0 28.036844 2 CAACGCA 17465 0.0 27.93471 5 ACATGGG 15565 0.0 27.783241 3 AACGCAG 17695 0.0 27.773355 6 CATGGGG 6735 0.0 24.498392 4 GAGTACT 10070 0.0 24.489878 12-13 ACGCAGA 20280 0.0 24.08652 7 AGTACTT 10580 0.0 23.731129 12-13 CGCAGAG 20385 0.0 23.670586 8 GTACTTT 10790 0.0 22.910849 14-15 TACCTGG 2460 0.0 22.518694 2 GCAGAGT 21680 0.0 21.515347 9 CATGGGA 10530 0.0 21.439085 4 TACTTTT 12450 0.0 20.190586 14-15 >>END_MODULE