FastQCFastQC Report
Thu 26 May 2016
SRR1364538_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364538_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2498912
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30260.12109269954284105No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34150.059.9372141
GTATCAA62500.052.361431
TCAACGC77050.042.0093884
ATCAACG76950.041.754693
CAACGCA77850.041.577695
AACGCAG81350.039.269796
TATCAAC84250.038.278022
GTACATG116600.036.895091
TACATGG115900.035.936412
ACATGGG120350.034.162693
ACGCAGA96250.032.6961677
CGCAGAG97450.031.4420438
CATGGGA63550.028.3692054
CATGGGG60800.026.5207524
GCAGAGT115650.025.362699
GTATAGG7750.023.0328871
AGAGTAC99150.022.9267710-11
CAGAGTA104250.022.86118310-11
TACCGTA1602.6155112E-422.3086417
GTGGTAT21750.022.1592241
Produced by FastQC (version 0.11.5)