##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364538_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2498912 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.375144062696084 31.0 28.0 33.0 16.0 34.0 2 28.29026072146598 31.0 28.0 34.0 16.0 34.0 3 28.50839845500762 31.0 28.0 34.0 16.0 34.0 4 31.858214294861124 35.0 32.0 37.0 19.0 37.0 5 31.958590378532737 35.0 32.0 37.0 19.0 37.0 6 31.946984127492286 35.0 32.0 37.0 19.0 37.0 7 31.78558708750048 35.0 32.0 37.0 17.0 37.0 8 31.670943594652393 35.0 32.0 37.0 17.0 37.0 9 32.787182181685466 37.0 32.0 39.0 16.0 39.0 10-11 32.70807255317514 37.0 32.0 39.0 14.0 39.0 12-13 32.62525371041477 37.0 32.0 39.0 12.5 39.0 14-15 33.34791361200394 38.0 32.0 41.0 10.5 41.0 16-17 33.450922641533595 38.0 32.0 41.0 11.0 41.0 18-19 33.24398538243844 38.0 32.0 41.0 10.0 41.0 20-21 32.958263636334536 38.0 31.5 40.5 10.0 41.0 22-23 32.86199694106875 38.0 31.5 40.0 10.0 41.0 24-25 32.67505838540933 38.0 31.0 40.0 9.0 41.0 26-27 32.578240050069795 38.0 31.0 40.0 9.0 41.0 28-29 32.64370414004175 38.0 31.0 40.0 8.5 41.0 30-31 32.57976131212304 38.0 31.0 40.0 8.0 41.0 32-33 32.560906706598715 38.0 31.0 40.0 8.0 41.0 34-35 32.09717188920618 38.0 30.0 40.0 4.0 41.0 36-37 31.958293649396218 38.0 30.0 40.0 2.0 41.0 38-39 31.752983698505588 37.0 30.0 40.0 2.0 41.0 40-41 31.946270016711274 37.5 30.0 40.0 2.0 41.0 42-43 31.832102130847346 37.0 30.0 40.0 2.0 41.0 44-45 31.67459038173413 37.0 30.0 40.0 2.0 41.0 46-47 31.632938254728458 37.0 30.0 40.0 2.0 41.0 48-49 31.472391984991866 37.0 30.0 40.0 2.0 41.0 50-51 31.30155643736154 37.0 30.0 40.0 2.0 41.0 52-53 31.221400353433815 37.0 29.5 40.0 2.0 41.0 54-55 31.236890294656234 37.0 30.0 40.0 2.0 41.0 56-57 31.095038560781653 36.5 29.5 40.0 2.0 41.0 58-59 30.87481652014957 36.0 29.0 40.0 2.0 41.0 60-61 30.655653940594945 36.0 29.0 40.0 2.0 41.0 62-63 30.356956347402388 35.0 29.0 39.0 2.0 41.0 64-65 30.061159416578093 35.0 28.0 39.0 2.0 41.0 66-67 29.804281023101254 35.0 28.0 39.0 2.0 40.0 68-69 29.75235582525515 35.0 29.0 38.5 2.0 40.0 70-71 29.51704901973339 35.0 29.0 37.5 2.0 40.0 72-73 29.242147982802116 35.0 28.5 37.0 2.0 39.5 74-75 28.95033898752737 34.5 28.0 36.5 2.0 39.0 76-77 28.659883981508752 34.0 28.0 36.0 2.0 39.0 78-79 28.368327296039237 34.0 28.0 36.0 2.0 38.5 80-81 28.093794219244216 34.0 27.5 35.0 2.0 37.0 82-83 27.864063640496344 34.0 27.0 35.0 2.0 37.0 84-85 27.635103597085454 34.0 27.0 35.0 2.0 36.0 86-87 27.45996157527756 34.0 26.5 35.0 2.0 36.0 88-89 27.272474981111777 34.0 26.0 35.0 2.0 36.0 90-91 27.148613876759164 34.0 26.0 35.0 2.0 35.5 92-93 27.03246853030439 34.0 26.0 35.0 2.0 35.0 94-95 26.91295251693537 34.0 26.0 35.0 2.0 35.0 96-97 26.809046096861355 34.0 25.5 35.0 2.0 35.0 98-99 26.71774276164987 34.0 25.0 35.0 2.0 35.0 100-101 26.623653013791603 34.0 25.0 35.0 2.0 35.0 102-103 25.441372285218527 31.5 23.5 34.0 2.0 35.0 104-105 26.33278922987284 33.0 25.0 35.0 2.0 35.0 106-107 26.4837603324967 33.5 25.0 35.0 2.0 35.0 108-109 26.4458470326286 34.0 25.0 35.0 2.0 35.0 110-111 26.354186341895993 34.0 25.0 35.0 2.0 35.0 112-113 26.26218870452421 33.5 24.5 35.0 2.0 35.0 114-115 26.14064100696623 33.0 24.0 35.0 2.0 35.0 116-117 26.033653045805536 33.0 24.0 35.0 2.0 35.0 118-119 25.883115932053627 33.0 23.0 35.0 2.0 35.0 120-121 25.712302994263105 33.0 22.0 35.0 2.0 35.0 122-123 25.47459854528691 33.0 18.5 35.0 2.0 35.0 124-125 24.046143281556134 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9490364271904568 1101 2 1.0667984481714932 1101 3 0.9806134000246587 1101 4 1.1139235800760083 1101 5 1.0047946450103673 1101 6 1.1821839832950651 1101 7 1.227293391278078 1101 8 1.0599048366158037 1101 9 1.4661539363321552 1101 10-11 1.510997273293036 1101 12-13 1.3359204293243963 1101 14-15 1.6280334822025395 1101 16-17 1.4458770407454011 1101 18-19 1.834595202096125 1101 20-21 2.1726667363027587 1101 22-23 2.007388594253918 1101 24-25 2.2712188786819425 1101 26-27 2.343036993664292 1101 28-29 2.0880170206206827 1101 30-31 2.1218291179377076 1101 32-33 2.08045953983558 1101 34-35 2.378799183815083 1101 36-37 2.25581063699639 1101 38-39 2.1115261576368987 1101 40-41 1.9891857446046188 1101 42-43 1.8964154297831577 1101 44-45 1.993093953194819 1101 46-47 1.8034938595564967 1101 48-49 1.8248856986865007 1101 50-51 1.824138225749401 1101 52-53 1.5535765840084785 1101 54-55 1.513040484129892 1101 56-57 1.5268276029670034 1101 58-59 1.6008009482773922 1101 60-61 1.39875005357786 1101 62-63 1.509494582098995 1101 64-65 1.4877782755544828 1101 66-67 1.5153215766692298 1101 68-69 1.4457377208850666 1101 70-71 1.349962027734449 1101 72-73 1.1459869504938105 1101 74-75 1.1764817613199874 1101 76-77 1.3226093916961048 1101 78-79 1.462152317388174 1101 80-81 1.4808444231804359 1101 82-83 1.2879300853804132 1101 84-85 1.3638453945050273 1101 86-87 1.3648988551852703 1101 88-89 1.36497374874196 1101 90-91 1.3477268711967554 1101 92-93 1.2367981779456052 1101 94-95 1.2438450177313598 1101 96-97 1.1936685123501718 1101 98-99 1.3231589800284667 1101 100-101 1.3884829000703505 1101 102-103 1.2762186687929358 1101 104-105 1.3576481215657346 1101 106-107 1.418745537288327 1101 108-109 1.4498201790172232 1101 110-111 1.2085034888073025 1101 112-113 1.1933690214321686 1101 114-115 1.3886917614513088 1101 116-117 1.4180458411072827 1101 118-119 1.4607385125537604 1101 120-121 1.4682424850442075 1101 122-123 1.4679432644314687 1101 124-125 1.6063419151538234 1102 1 0.11288302710364562 1102 2 0.37273391778323983 1102 3 0.3829844434911287 1102 4 0.4024788123327774 1102 5 0.42971264623394134 1102 6 -0.37873058360670697 1102 7 -0.9534605075800329 1102 8 0.026121497377264546 1102 9 0.26182517563870533 1102 10-11 0.22648373480801354 1102 12-13 0.29772962025081995 1102 14-15 0.41798125533336616 1102 16-17 0.3541081200760132 1102 18-19 0.568798866948697 1102 20-21 0.8789801721397765 1102 22-23 0.7020957070995024 1102 24-25 0.9235018425318486 1102 26-27 0.545753975699192 1102 28-29 0.9522472759760987 1102 30-31 0.9203971584090382 1102 32-33 0.7814802594083119 1102 34-35 1.106109497204777 1102 36-37 1.273848765715389 1102 38-39 1.2309040862663458 1102 40-41 1.0271835902498268 1102 42-43 0.9377078233499354 1102 44-45 1.1259431500682133 1102 46-47 0.7737478706324552 1102 48-49 0.541874853999424 1102 50-51 0.8376159023728889 1102 52-53 0.6716850482531349 1102 54-55 0.5407287067814899 1102 56-57 0.42458692730164316 1102 58-59 0.6534315809581877 1102 60-61 0.5730073152687396 1102 62-63 0.5013962060678168 1102 64-65 0.5915164827120414 1102 66-67 0.4902652754947603 1102 68-69 0.4733131204546517 1102 70-71 0.40645103893002954 1102 72-73 0.5692294452845097 1102 74-75 0.1796344704410764 1102 76-77 0.35386950269493767 1102 78-79 0.32123878009938167 1102 80-81 0.013308312673768086 1102 82-83 0.21894975397890093 1102 84-85 -0.10125261240349204 1102 86-87 0.37797561646430466 1102 88-89 0.5863339529328719 1102 90-91 0.06684197081999699 1102 92-93 0.484518468128428 1102 94-95 0.3442427858494135 1102 96-97 0.5010086631550053 1102 98-99 0.5754015568464226 1102 100-101 0.603527931041338 1102 102-103 0.3862857559822146 1102 104-105 0.6802105591196401 1102 106-107 0.5695474534222953 1102 108-109 0.16206072509090674 1102 110-111 0.47076510420883366 1102 112-113 0.14859022018080026 1102 114-115 0.18292278866043432 1102 116-117 0.13952225058052292 1102 118-119 0.4691853976255942 1102 120-121 0.8415152131454917 1102 122-123 0.6429063023223662 1102 124-125 0.9207057923430675 1103 1 0.6482485053436946 1103 2 0.6161717277642005 1103 3 0.5849158742777263 1103 4 0.7186106490162807 1103 5 0.8105197653920477 1103 6 0.7971768411142399 1103 7 0.8416755704981149 1103 8 0.8181875525381948 1103 9 1.2264209319637445 1103 10-11 1.253345330803775 1103 12-13 1.1640983251432502 1103 14-15 1.5124906730537333 1103 16-17 1.3071687366744627 1103 18-19 1.4630012253693465 1103 20-21 1.7487683589435647 1103 22-23 1.7446508195762718 1103 24-25 1.845228016041375 1103 26-27 1.7634948629715765 1103 28-29 1.7249028935436783 1103 30-31 1.655567526794691 1103 32-33 1.7674180266332726 1103 34-35 1.98012489791396 1103 36-37 2.001569400898436 1103 38-39 2.007656369436443 1103 40-41 1.6874618462613213 1103 42-43 1.6699981479534465 1103 44-45 1.2821491826539848 1103 46-47 1.3636511850771385 1103 48-49 1.2013740957931205 1103 50-51 1.2738595887377215 1103 52-53 1.2839615056624716 1103 54-55 1.101245327461708 1103 56-57 1.174847240047491 1103 58-59 1.0710328699089011 1103 60-61 1.0629001544948018 1103 62-63 0.9588858448651756 1103 64-65 1.1228120178460692 1103 66-67 1.1892112689730467 1103 68-69 1.1716273941158164 1103 70-71 1.1172298251158566 1103 72-73 1.144862661561433 1103 74-75 1.107402636037893 1103 76-77 1.1741738256110068 1103 78-79 1.0647695843681397 1103 80-81 1.1247709809898652 1103 82-83 1.197320707836628 1103 84-85 1.1043264035245386 1103 86-87 1.0897103554247067 1103 88-89 1.1536155695655204 1103 90-91 1.225145897530524 1103 92-93 1.2430265547121628 1103 94-95 1.1450231540070313 1103 96-97 1.2829732583412508 1103 98-99 1.1388535903215171 1103 100-101 1.1758309541751828 1103 102-103 1.1301797011633283 1103 104-105 1.0348309143934493 1103 106-107 1.1843443054409448 1103 108-109 1.3060130322043655 1103 110-111 1.305686703967119 1103 112-113 1.3187954917098672 1103 114-115 1.4265788679927596 1103 116-117 1.5298932443438495 1103 118-119 1.511664475692342 1103 120-121 1.5381067052726891 1103 122-123 1.5808238146075908 1103 124-125 1.566649032335313 1104 1 0.8123906937782799 1104 2 0.7338648504510239 1104 3 0.7009753229195397 1104 4 0.7688791967761368 1104 5 0.6928574680277144 1104 6 0.7457587742089977 1104 7 0.7941133907871638 1104 8 0.837083951555325 1104 9 1.1442626694503986 1104 10-11 1.0854241483634581 1104 12-13 0.9906258967951089 1104 14-15 1.0975509729643917 1104 16-17 1.2297803695931862 1104 18-19 1.3256315780368908 1104 20-21 1.5592641771109044 1104 22-23 1.3582827570600742 1104 24-25 1.7054278975033768 1104 26-27 1.758255953112858 1104 28-29 1.6863932196320448 1104 30-31 1.4857964167224296 1104 32-33 1.4943936882379205 1104 34-35 1.6421290474242554 1104 36-37 1.7087665693237888 1104 38-39 1.775046716687445 1104 40-41 1.5210170470648237 1104 42-43 1.4872166423303774 1104 44-45 1.4156179636791997 1104 46-47 1.3028761697835591 1104 48-49 1.2930203313271633 1104 50-51 1.005249225577316 1104 52-53 1.1259858447382918 1104 54-55 1.0489801766115612 1104 56-57 0.950451889295465 1104 58-59 0.9135493594150184 1104 60-61 0.7807516567359265 1104 62-63 0.8257421587145899 1104 64-65 0.8944139343237332 1104 66-67 0.8690275895182928 1104 68-69 0.9082405621764273 1104 70-71 0.8648136778184465 1104 72-73 0.7473442701397168 1104 74-75 0.8810506258253881 1104 76-77 0.7918590548377722 1104 78-79 0.9204881239963711 1104 80-81 0.8904565450082096 1104 82-83 0.9240725286060929 1104 84-85 0.8981022741697835 1104 86-87 0.8850003073902784 1104 88-89 0.9703518483843077 1104 90-91 0.9264290270969084 1104 92-93 0.9444761614341282 1104 94-95 0.8817957563515257 1104 96-97 0.8346511629814515 1104 98-99 0.8834542254728603 1104 100-101 0.8697032815414687 1104 102-103 1.0066345304978768 1104 104-105 0.9622054679196133 1104 106-107 0.9468514248655922 1104 108-109 0.9981327052245881 1104 110-111 0.9730898100436534 1104 112-113 1.0384707915082458 1104 114-115 0.9320476408523177 1104 116-117 0.9197831169665385 1104 118-119 0.9602445715162204 1104 120-121 0.9625679255880044 1104 122-123 0.9591403777406384 1104 124-125 1.2565543176192513 1105 1 0.1352423977296091 1105 2 0.46121031625683884 1105 3 0.4828567145168279 1105 4 0.5422452791136045 1105 5 0.4938902223754802 1105 6 0.45941792144295235 1105 7 0.49317496163410723 1105 8 0.354268013390989 1105 9 0.3876478488350017 1105 10-11 0.6190636107511054 1105 12-13 0.6394564774493716 1105 14-15 0.589542192367638 1105 16-17 0.6483648071468266 1105 18-19 0.6883540581624814 1105 20-21 1.1895034614272078 1105 22-23 1.3422976296642375 1105 24-25 1.4429811486246393 1105 26-27 1.2040480855460984 1105 28-29 0.9497734125316342 1105 30-31 0.8472930751619359 1105 32-33 0.9455845429757019 1105 34-35 1.5955091327748647 1105 36-37 1.4752395395920352 1105 38-39 1.5695178330547002 1105 40-41 1.228786974217634 1105 42-43 0.7836516775689084 1105 44-45 1.0442549034005602 1105 46-47 1.0541166526284442 1105 48-49 0.8914994872177999 1105 50-51 0.9318762303428159 1105 52-53 0.9203483636870367 1105 54-55 0.6891378109559732 1105 56-57 0.6580842687369461 1105 58-59 0.6310574413153986 1105 60-61 0.6938968889237032 1105 62-63 0.5662580799890051 1105 64-65 0.6215195456007123 1105 66-67 0.49322187682735574 1105 68-69 0.3824408120282783 1105 70-71 0.4336445885601634 1105 72-73 0.5384188678067403 1105 74-75 0.5860726425907217 1105 76-77 0.6448400658131135 1105 78-79 0.6506216788670827 1105 80-81 0.5603690941552202 1105 82-83 0.4857999979899752 1105 84-85 0.426450698519659 1105 86-87 0.4982096157752238 1105 88-89 0.6891831548251162 1105 90-91 0.46955424232170273 1105 92-93 0.4661784305350807 1105 94-95 0.5470074678813361 1105 96-97 0.6368951285871489 1105 98-99 0.49763712273564664 1105 100-101 0.5289482565518853 1105 102-103 0.4647803887019144 1105 104-105 0.5890087989078374 1105 106-107 0.4520212842828357 1105 108-109 0.4043687944566976 1105 110-111 0.520491179676597 1105 112-113 0.5906639757299814 1105 114-115 0.5653807171503402 1105 116-117 0.6112276831420012 1105 118-119 0.6590317233492158 1105 120-121 0.5674206778375535 1105 122-123 0.71920633705966 1105 124-125 0.9187802596384422 1106 1 0.29048382874290013 1106 2 0.45757253484021376 1106 3 0.23217663511888986 1106 4 0.37013058702048696 1106 5 0.26776900544028237 1106 6 0.20290765995924787 1106 7 0.049919245955099 1106 8 0.4169375706363887 1106 9 0.6266010176078396 1106 10-11 0.6348755210037282 1106 12-13 0.5959663482896289 1106 14-15 0.8482753521227764 1106 16-17 0.6918827610853029 1106 18-19 0.821662641606423 1106 20-21 1.0755435848952004 1106 22-23 1.0273568063208103 1106 24-25 1.292821714698519 1106 26-27 1.4261518087824854 1106 28-29 1.2366441741787497 1106 30-31 0.9199571764205601 1106 32-33 0.8886054956508929 1106 34-35 1.1545288123636297 1106 36-37 1.1393841801935487 1106 38-39 1.3478804147308772 1106 40-41 0.9352981986031565 1106 42-43 0.944369244345463 1106 44-45 0.6525649791710002 1106 46-47 0.47975630214315146 1106 48-49 0.6614516525852565 1106 50-51 0.7016498648023912 1106 52-53 0.6841298360989434 1106 54-55 0.47267689801949686 1106 56-57 0.48058164405846426 1106 58-59 0.3675873185827676 1106 60-61 0.5367189629648941 1106 62-63 0.4445137418688745 1106 64-65 0.5087046655669738 1106 66-67 0.39696808578692355 1106 68-69 0.427802911431133 1106 70-71 0.43391067157353547 1106 72-73 0.3812147276167366 1106 74-75 0.4937636650081032 1106 76-77 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2216 94-95 -2.4845057831197686 2216 96-97 -2.3761965607579505 2216 98-99 -2.43011143517883 2216 100-101 -2.4684679612497433 2216 102-103 -2.3461991585678987 2216 104-105 -2.4831304967209213 2216 106-107 -2.446650026662528 2216 108-109 -2.5105102085049253 2216 110-111 -2.534982463038773 2216 112-113 -2.5464323131340336 2216 114-115 -2.598893932067398 2216 116-117 -2.5919866901097564 2216 118-119 -2.6560734346532584 2216 120-121 -2.868574204598424 2216 122-123 -2.868852407451577 2216 124-125 -2.8605043664579917 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 161851.0 3 28122.0 4 17539.0 5 13764.0 6 12696.0 7 13301.0 8 14193.0 9 14628.0 10 15604.0 11 16681.0 12 17531.0 13 19270.0 14 20756.0 15 18747.0 16 14824.0 17 13175.0 18 12307.0 19 11738.0 20 11905.0 21 12520.0 22 15148.0 23 20101.0 24 26718.0 25 33928.0 26 41165.0 27 47113.0 28 54957.0 29 63523.0 30 72444.0 31 83819.0 32 103107.0 33 129421.0 34 166840.0 35 229124.0 36 354355.0 37 448878.0 38 146696.0 39 423.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.87451688389965 18.36179367595672 13.037701165802124 23.725988274341507 2 15.291121388921013 19.858868426202466 40.39710213250104 24.452908052375477 3 19.516951713180575 25.184784979479453 28.1886489655683 27.10961434177167 4 12.393910300150758 16.142603126171853 37.120179164377156 34.34330740930023 5 13.55407762991851 38.531234353406205 33.66568649699205 14.24900151968324 6 32.32233576174974 36.305072062139715 17.401601114096888 13.970991062013658 7 29.212725451423115 32.360470947197136 20.414422007524685 18.012381593855068 8 26.537429089139593 35.53614533044781 19.21188101061582 18.714544569796775 9 26.81922757237432 16.659269927874792 19.231494179920436 37.29000831983045 10-11 25.058045261297718 26.948507990677545 26.886260900744002 21.10718584728074 12-13 25.955490991748103 24.027961197685308 27.241878166494587 22.774669644072002 14-15 22.863668684004658 25.527847649588768 24.858705956493782 26.749777709912788 16-17 22.71097975160404 28.976459966251007 25.50761093665969 22.804949345485266 18-19 22.787297942319803 27.524366580950836 27.41008992258362 22.278245554145744 20-21 23.414229447850552 26.629743444437175 27.669104535821347 22.286922571890926 22-23 23.17252959744908 26.49531947316861 27.505318416688546 22.826832512693766 24-25 22.87678850760608 26.676416022963018 27.650751322776735 22.79604414665417 26-27 22.464125877778525 27.07885592654728 27.42113176569145 23.035886429982746 28-29 22.735215324044034 27.06638710326738 27.48663259962948 22.711764973059108 30-31 22.714335965590834 26.83019798156686 27.300050542865595 23.155415509976713 32-33 22.564032578684262 26.9040484830245 27.243946725662937 23.2879722126283 34-35 23.037360586166432 26.802474590543152 27.414918941071193 22.745245882219223 36-37 23.178551381811378 26.42143821245506 27.273287581457385 23.12672282427618 38-39 22.78897143375083 26.772407415151523 27.332451482356014 23.10616966874164 40-41 23.06039481876671 26.756489600568067 27.268880998588834 22.914234582076386 42-43 22.79797764329729 26.65116908241948 27.030531054733544 23.520322219549687 44-45 22.862738238991646 26.858433237048473 27.207244058308845 23.07158446565104 46-47 23.30440882918388 27.11845346813916 26.513103868890873 23.064033833786084 48-49 23.179873309571793 27.07439014895962 26.43741560492568 23.30832093654291 50-51 23.17850250269879 27.247831711389757 26.50999828918052 23.063667496730933 52-53 23.393460834155025 26.92531789834936 26.507656131948625 23.17356513554699 54-55 23.188798789664116 27.077823316576477 26.531951426467295 23.201426467292112 56-57 23.134381667603453 27.388121328347186 26.260095138065854 23.217401865983508 58-59 23.06623839917116 26.837589496681645 26.554145404858247 23.542026699288947 60-61 22.960308500450417 27.131655752748223 26.713034312110988 23.195001434690372 62-63 22.980134783102958 27.093697312823927 26.92880096195876 22.997366942114354 64-65 22.94351060589505 27.01056126772035 26.700091135811572 23.345836990573027 66-67 22.885193956599377 27.195783033689793 26.575419651696897 23.34360335801394 68-69 23.111095901294696 26.943829547590013 26.80095733786586 23.144117213249434 70-71 23.0856763937406 26.868502216834816 26.697382338075414 23.34843905134917 72-73 22.95669178126189 27.028790167026635 26.560890669965566 23.453627381745918 74-75 22.841585226207492 27.45293300703126 26.373623075487068 23.33185869127418 76-77 23.27442928532752 27.063459253475635 26.54482055649358 23.117290904703268 78-79 23.18150213145276 27.101494367982486 26.52649734431291 23.190506156251843 80-81 23.126507959947542 27.295570754711317 26.4404281535747 23.137493131766433 82-83 23.153897977342925 27.295235659848178 26.477484891649873 23.073381471159024 84-85 23.01615663136597 27.436860521699042 26.4538727254101 23.093110121524887 86-87 23.209719527278192 27.361232865775413 26.397696300590273 23.031351306356125 88-89 23.14826636803212 27.328823129875207 26.31973081265712 23.20317968943555 90-91 22.98788464990727 27.420373248925944 26.42580061036052 23.165941490806265 92-93 23.01850095446196 27.221455184309207 26.504107988266323 23.255935872962514 94-95 22.95178820384671 27.05313516570741 26.677978408422724 23.31709822202315 96-97 23.275510300059256 26.965668603795983 26.55349412727418 23.20532696887058 98-99 22.925039982824345 27.182297868389725 26.410613851655068 23.482048297130863 100-101 22.870086795567673 26.981125090672602 26.695094924835537 23.453693188924184 102-103 22.850304452497728 26.647176851365717 26.868193037609966 23.634325658526592 104-105 22.605223308399456 27.08637803556479 26.680375496823373 23.628023159212383 106-107 22.859477588437525 27.002422255134334 26.445379607356966 23.692720549071176 108-109 22.851605018504053 27.002591527832916 26.330418998348083 23.815384455314952 110-111 22.81731437988166 26.99066066827965 26.674520808005465 23.517504143833232 112-113 22.80572505154243 27.11536060493527 26.733114251322178 23.345800092200125 114-115 22.705601477763125 27.378134964336482 26.463697000934804 23.452566556965593 116-117 22.66250272118426 27.322390704434568 26.67616946895289 23.338937105428283 118-119 22.59053139926496 27.357325908235264 26.467818794739472 23.584323897760306 120-121 22.513102204699926 27.43120354602394 26.574530550601732 23.481163698674404 122-123 22.488246885044372 27.446604762392596 26.57552566877105 23.489622683791985 124-125 22.5516366585252 27.548270399091873 26.38178745574029 23.518305486642635 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 989.0 1 1018.5 2 1272.5 3 1344.0 4 1047.5 5 803.5 6 721.5 7 858.0 8 1128.5 9 1242.5 10 1101.5 11 1007.0 12 1086.5 13 1172.0 14 1275.0 15 1428.5 16 1573.0 17 1695.5 18 1850.5 19 2134.0 20 2514.5 21 3039.0 22 3798.5 23 4838.5 24 6100.5 25 7820.0 26 10587.0 27 14209.5 28 17679.0 29 21458.5 30 25920.0 31 31722.5 32 39173.0 33 44048.0 34 46848.5 35 51100.5 36 57259.0 37 62826.5 38 65640.5 39 67886.5 40 70344.0 41 72633.0 42 73120.5 43 74884.5 44 79050.5 45 82395.5 46 84230.0 47 85939.0 48 87891.0 49 88955.0 50 88855.0 51 87415.5 52 88506.0 53 92123.5 54 93145.0 55 89436.5 56 84124.5 57 78808.0 58 73538.0 59 66255.5 60 55105.5 61 44623.5 62 36692.5 63 27906.0 64 19843.5 65 15106.5 66 11643.0 67 9086.5 68 7153.5 69 5581.0 70 4195.0 71 2946.5 72 2233.5 73 1791.5 74 1369.5 75 1005.5 76 714.0 77 466.5 78 312.0 79 220.0 80 148.0 81 93.0 82 57.5 83 38.5 84 31.0 85 24.5 86 23.5 87 17.5 88 13.5 89 12.0 90 13.0 91 8.5 92 6.0 93 7.0 94 6.5 95 8.0 96 7.0 97 4.5 98 3.0 99 3.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011565033102406167 2 0.00436189829813935 3 0.00788343086911424 4 0.00804350053143128 5 0.014606356686429933 6 0.0010804702206400226 7 0.0081235353625898 8 0.0 9 0.0025210971814933858 10-11 0.0 12-13 0.0036816022332919287 14-15 0.019508490094889294 16-17 0.03055329679476508 18-19 0.006062638460257904 20-21 0.00854371822617203 22-23 0.0019208359478044845 24-25 0.011424972147878756 26-27 0.019548507510468556 28-29 6.202699414785314E-4 30-31 0.001720748869908184 32-33 0.008923883674175001 34-35 0.009444110076705382 36-37 0.011585041810195797 38-39 6.002612336889014E-5 40-41 0.02507091086040645 42-43 0.009724231985760202 44-45 0.01692736679002702 46-47 0.00432188088256009 48-49 0.024710754120193107 50-51 0.00438190700592898 52-53 0.0 54-55 0.01728752353024036 56-57 0.004902133408459362 58-59 2.601132012651906E-4 60-61 0.004602002791614911 62-63 0.027051772931579825 64-65 0.01772771510161222 66-67 0.002160940441280045 68-69 0.021029151886901177 70-71 0.0031613758307615474 72-73 0.011945198550409139 74-75 2.0008707789630046E-5 76-77 0.006362769077102355 78-79 0.0011805137595881727 80-81 0.0035615499865541485 82-83 0.0018207924088563343 84-85 0.0 86-87 0.005942586213520124 88-89 0.017167471283502583 90-91 0.0221096221075412 92-93 0.004882124700669731 94-95 0.02493084990587904 96-97 0.018227932796352975 98-99 0.0010604615128503925 100-101 0.00898390979754389 102-103 0.0 104-105 0.0014406269608533634 106-107 4.001741557926009E-5 108-109 0.0 110-111 7.203134804266817E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 2.0008707789630046E-5 122-123 0.0 124-125 0.023610275191763456 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2498912.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.88760271201492 #Duplication Level Percentage of deduplicated Percentage of total 1 76.6530375126512 41.30648432150924 2 12.001319473825408 12.934446716509425 3 4.464770848502391 7.217873930528477 4 2.1930516010944907 4.727131736269125 5 1.1960999973338786 3.222748073008507 6 0.7813298299328617 2.5262394877480934 7 0.5034178161192605 1.8989585491229417 8 0.3697336804773595 1.593924934625201 9 0.2783864280277191 1.3501419510577195 >10 1.4125988152954545 13.647609753608055 >50 0.09991169105737122 3.6649374363857734 >100 0.04284712810867338 4.098999594049932 >500 0.0024314278774323506 0.9090596375430905 >1k 0.0010637496963748095 0.9014438780345437 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3026 0.12109269954284105 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.2005224673778028E-4 2 0.0 0.0 0.0 0.0 1.2005224673778028E-4 3 0.0 0.0 0.0 0.0 1.2005224673778028E-4 4 0.0 0.0 0.0 0.0 1.2005224673778028E-4 5 0.0 0.0 0.0 0.0 1.2005224673778028E-4 6 0.0 0.0 0.0 0.0 1.2005224673778028E-4 7 0.0 0.0 0.0 0.0 1.2005224673778028E-4 8 0.0 0.0 0.0 0.0 1.2005224673778028E-4 9 0.0 0.0 0.0 0.0 1.6006966231704037E-4 10-11 0.0 0.0 0.0 0.0 1.6006966231704037E-4 12-13 0.0 0.0 0.0 0.0 2.0008707789630046E-4 14-15 0.0 0.0 0.0 4.001741557926009E-5 2.0008707789630046E-4 16-17 0.0 0.0 0.0 4.001741557926009E-5 2.0008707789630046E-4 18-19 0.0 0.0 0.0 4.001741557926009E-5 2.0008707789630046E-4 20-21 4.001741557926009E-5 0.0 0.0 1.4006095452741033E-4 2.4010449347556056E-4 22-23 8.003483115852018E-5 0.0 0.0 3.6015674021334086E-4 2.4010449347556056E-4 24-25 8.003483115852018E-5 0.0 0.0 6.602873570577916E-4 2.4010449347556056E-4 26-27 8.003483115852018E-5 0.0 0.0 9.003918505333521E-4 3.2013932463408073E-4 28-29 8.003483115852018E-5 0.0 0.0 0.0011805137595881727 3.2013932463408073E-4 30-31 8.003483115852018E-5 0.0 0.0 0.0028212277983378367 3.401480324237108E-4 32-33 8.003483115852018E-5 0.0 0.0 0.006522838739419395 4.0017415579260093E-4 34-35 8.003483115852018E-5 0.0 0.0 0.010564597712924666 4.0017415579260093E-4 36-37 8.003483115852018E-5 0.0 0.0 0.01766768897824333 4.0017415579260093E-4 38-39 8.003483115852018E-5 0.0 0.0 0.031493706060877695 4.0017415579260093E-4 40-41 8.003483115852018E-5 0.0 0.0 0.04880123829890769 4.0017415579260093E-4 42-43 8.003483115852018E-5 0.0 0.0 0.06716923204978807 4.0017415579260093E-4 44-45 8.003483115852018E-5 0.0 0.0 0.08825841006005813 4.0017415579260093E-4 46-47 8.003483115852018E-5 0.0 0.0 0.11476994788131795 4.40191571371861E-4 48-49 8.003483115852018E-5 0.0 0.0 0.14332237389712005 5.202264025303812E-4 50-51 8.003483115852018E-5 0.0 0.0 0.17963817853529856 5.202264025303812E-4 52-53 8.003483115852018E-5 0.0 0.0 0.21487351295283708 5.202264025303812E-4 54-55 8.003483115852018E-5 0.0 0.0 0.2509091956819608 5.202264025303812E-4 56-57 8.003483115852018E-5 0.0 0.0 0.29658907556568614 5.402351103200113E-4 58-59 8.003483115852018E-5 0.0 0.0 0.3466508624553406 5.602438181096413E-4 60-61 8.003483115852018E-5 0.0 0.0 0.40557650689580105 5.602438181096413E-4 62-63 8.003483115852018E-5 0.0 0.0 0.46446213392068225 6.402786492681615E-4 64-65 8.003483115852018E-5 0.0 0.0 0.5254686839712643 6.402786492681615E-4 66-67 8.003483115852018E-5 0.0 0.0 0.5986805457735206 6.802960648474216E-4 68-69 8.003483115852018E-5 0.0 0.0 0.6859385204440973 6.802960648474216E-4 70-71 8.003483115852018E-5 0.0 0.0 0.7839211624899156 7.003047726370517E-4 72-73 8.003483115852018E-5 0.0 0.0 0.8867259031130348 8.403657271644619E-4 74-75 8.003483115852018E-5 0.0 0.0 0.9883301212687761 8.803831427437221E-4 76-77 8.003483115852018E-5 0.0 0.0 1.1039804522928378 9.003918505333521E-4 78-79 8.003483115852018E-5 0.0 0.0 1.243781293618983 9.204005583229822E-4 80-81 8.003483115852018E-5 0.0 0.0 1.4011297716766338 9.204005583229822E-4 82-83 8.003483115852018E-5 0.0 0.0 1.5673821247006696 9.604179739022422E-4 84-85 8.003483115852018E-5 0.0 0.0 1.7346749305297666 9.804266816918724E-4 86-87 8.003483115852018E-5 0.0 0.0 1.9105714807084042 0.0010604615128503925 88-89 8.003483115852018E-5 0.0 0.0 2.11580079650664 0.0011204876362192826 90-91 8.003483115852018E-5 0.0 0.0 2.3496625731518357 0.0011204876362192826 92-93 8.003483115852018E-5 0.0 0.0 2.601252064898644 0.0011805137595881727 94-95 8.003483115852018E-5 0.0 0.0 2.8516210254702847 0.0012005224673778028 96-97 8.003483115852018E-5 0.0 0.0 3.10931317309293 0.0013405834219052132 98-99 8.003483115852018E-5 4.001741557926009E-5 0.0 3.3921962838227193 0.0014406269608533634 100-101 8.003483115852018E-5 4.001741557926009E-5 0.0 3.7323843336620097 0.0014406269608533634 102-103 8.003483115852018E-5 4.001741557926009E-5 0.0 4.090600229219756 0.0014406269608533634 104-105 8.003483115852018E-5 4.001741557926009E-5 0.0 4.45493878936113 0.0014406269608533634 106-107 8.003483115852018E-5 4.001741557926009E-5 0.0 4.814515277048572 0.0014406269608533634 108-109 8.003483115852018E-5 4.001741557926009E-5 0.0 5.2016637640701235 0.0014406269608533634 110-111 8.003483115852018E-5 4.001741557926009E-5 0.0 5.642735718584728 0.0015206617920118836 112-113 8.003483115852018E-5 4.001741557926009E-5 0.0 6.113060403887772 0.0015606792075911436 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3415 0.0 59.937214 1 GTATCAA 6250 0.0 52.36143 1 TCAACGC 7705 0.0 42.009388 4 ATCAACG 7695 0.0 41.75469 3 CAACGCA 7785 0.0 41.57769 5 AACGCAG 8135 0.0 39.26979 6 TATCAAC 8425 0.0 38.27802 2 GTACATG 11660 0.0 36.89509 1 TACATGG 11590 0.0 35.93641 2 ACATGGG 12035 0.0 34.16269 3 ACGCAGA 9625 0.0 32.696167 7 CGCAGAG 9745 0.0 31.442043 8 CATGGGA 6355 0.0 28.369205 4 CATGGGG 6080 0.0 26.520752 4 GCAGAGT 11565 0.0 25.36269 9 GTATAGG 775 0.0 23.032887 1 AGAGTAC 9915 0.0 22.92677 10-11 CAGAGTA 10425 0.0 22.861183 10-11 TACCGTA 160 2.6155112E-4 22.308641 7 GTGGTAT 2175 0.0 22.159224 1 >>END_MODULE