FastQCFastQC Report
Thu 26 May 2016
SRR1364538_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364538_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2498912
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74110.2965690668578966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64060.2563515642007402No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41860.16751290161478274No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46550.065.8235861
GTATCAA84250.054.1289521
ATCAACG110950.040.9584663
TCAACGC112650.040.5518464
CAACGCA113500.039.9965935
TATCAAC114650.039.1171652
AACGCAG119150.038.0999836
GTACATG116350.033.6105421
TACATGG115400.032.8760832
ACGCAGA141700.031.826857
CGCAGAG143400.031.3665668
ACATGGG120000.030.722453
GCAGAGT159650.027.4285769
GTGGTAT24600.026.6560421
GAGTACT94300.025.23728612-13
CATGGGG59350.025.1883344
TGGTATC24700.025.077422
TAAGGTG13900.024.8260445
CAGAGTA149750.024.43371810-11
AGAGTAC146000.024.40929610-11
Produced by FastQC (version 0.11.5)