##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364538_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2498912 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.08230902088589 31.0 30.0 34.0 26.0 34.0 2 29.996370020232806 31.0 30.0 34.0 26.0 34.0 3 30.27740072479543 31.0 30.0 34.0 26.0 34.0 4 33.835971414759705 35.0 35.0 37.0 30.0 37.0 5 33.26508976706663 35.0 33.0 37.0 27.0 37.0 6 33.28980172170929 36.0 33.0 37.0 27.0 37.0 7 33.075033454559424 36.0 33.0 37.0 26.0 37.0 8 32.997860268788976 36.0 33.0 37.0 26.0 37.0 9 34.246595318282516 38.0 34.0 39.0 25.0 39.0 10-11 34.17881361968729 38.0 34.0 39.0 25.0 39.0 12-13 34.05937844149774 38.0 34.0 39.0 25.0 39.0 14-15 35.02522897965194 39.0 34.0 41.0 25.0 41.0 16-17 35.03127541105889 39.0 34.0 41.0 25.0 41.0 18-19 35.04626813589274 39.0 34.0 41.0 25.0 41.0 20-21 35.05757845814499 39.0 34.0 41.0 25.0 41.0 22-23 35.01367935325454 39.0 34.0 41.0 25.0 41.0 24-25 34.96390869306322 39.0 34.0 41.0 24.5 41.0 26-27 34.83607626038852 39.0 34.0 41.0 24.0 41.0 28-29 34.68689253563151 39.0 34.0 41.0 24.0 41.0 30-31 34.52211782567773 38.5 33.5 41.0 23.5 41.0 32-33 34.3173909285321 38.0 33.0 40.5 22.5 41.0 34-35 34.1316026734835 38.0 33.0 40.0 21.5 41.0 36-37 33.9246794204838 38.0 33.0 40.0 19.0 41.0 38-39 33.75360977097233 38.0 33.0 40.0 18.0 41.0 40-41 33.77580443008797 38.0 33.0 40.0 18.0 41.0 42-43 33.635720465546605 38.0 33.0 40.0 15.5 41.0 44-45 33.477524618714064 38.0 33.0 40.0 15.0 41.0 46-47 33.23317687857756 38.0 32.0 40.0 11.0 41.0 48-49 32.946025510302086 38.0 32.0 40.0 8.5 41.0 50-51 32.76617744042207 38.0 32.0 40.0 7.5 41.0 52-53 32.56892259511339 38.0 31.0 40.0 6.0 41.0 54-55 32.36980573945782 37.0 31.0 40.0 2.0 41.0 56-57 32.144848438040235 37.0 31.0 40.0 2.0 41.0 58-59 31.91640441920324 36.5 31.0 40.0 2.0 41.0 60-61 31.67330642295527 36.0 30.5 40.0 2.0 41.0 62-63 31.483584055781076 36.0 30.0 39.5 2.0 41.0 64-65 31.332454284104443 35.5 30.0 39.0 2.0 41.0 66-67 31.022590431355727 35.0 30.0 39.0 2.0 41.0 68-69 30.7275324221101 35.0 30.0 38.5 2.0 40.5 70-71 30.409370958241027 35.0 30.0 37.5 2.0 40.0 72-73 30.12462323603232 35.0 29.0 37.0 2.0 39.5 74-75 29.786363425362715 35.0 29.0 36.5 2.0 39.0 76-77 29.48750816355278 35.0 29.0 36.0 2.0 39.0 78-79 29.21126574285129 34.0 29.0 36.0 2.0 38.5 80-81 28.951642554839864 34.0 29.0 35.0 2.0 37.0 82-83 28.68275093320613 34.0 29.0 35.0 2.0 37.0 84-85 28.471670470988975 34.0 29.0 35.0 2.0 36.0 86-87 28.273822567581412 34.0 28.5 35.0 2.0 36.0 88-89 28.101050577211204 34.0 28.0 35.0 2.0 36.0 90-91 27.97114284136456 34.0 28.0 35.0 2.0 35.5 92-93 27.86628680801885 34.0 27.5 35.0 2.0 35.0 94-95 27.75735700176717 34.0 27.0 35.0 2.0 35.0 96-97 27.646906533723474 34.0 27.0 35.0 2.0 35.0 98-99 27.56147775511903 34.0 27.0 35.0 2.0 35.0 100-101 27.461640906122344 34.0 27.0 35.0 2.0 35.0 102-103 26.180329079215277 31.5 24.5 34.0 2.0 35.0 104-105 27.132236749433353 33.5 25.5 35.0 2.0 35.0 106-107 27.29881244317527 34.0 27.0 35.0 2.0 35.0 108-109 27.305383302813382 34.0 27.0 35.0 2.0 35.0 110-111 27.2176999430152 34.0 27.0 35.0 2.0 35.0 112-113 27.131067840724285 34.0 26.5 35.0 2.0 35.0 114-115 27.034269514092532 34.0 26.0 35.0 2.0 35.0 116-117 26.913182817162028 34.0 25.0 35.0 2.0 35.0 118-119 26.773840175244267 34.0 25.0 35.0 2.0 35.0 120-121 26.578517370759755 34.0 25.0 35.0 2.0 35.0 122-123 26.29058066070354 34.0 25.0 35.0 2.0 35.0 124-125 24.49935331856424 31.5 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.72911707668678 1101 2 0.9051798085822611 1101 3 0.7057398447818848 1101 4 0.766737876993254 1101 5 0.7558760435917264 1101 6 0.690855037442148 1101 7 0.8768722035375234 1101 8 0.8835183125357204 1101 9 1.1440905142346622 1101 10-11 1.0803132471424988 1101 12-13 0.9795628300793027 1101 14-15 1.1236354021468316 1101 16-17 1.2488984066990696 1101 18-19 1.2743211683417286 1101 20-21 1.28935901469427 1101 22-23 1.2246975664169284 1101 24-25 1.3001028517213626 1101 26-27 1.310237065639349 1101 28-29 1.3329521622788576 1101 30-31 1.3183784442695057 1101 32-33 1.3280562440748156 1101 34-35 1.383686510691085 1101 36-37 1.4209466329314182 1101 38-39 1.4067578921086508 1101 40-41 1.245419691848575 1101 42-43 1.2941388222552135 1101 44-45 1.2148596897768869 1101 46-47 1.253239906805291 1101 48-49 1.4291016673963242 1101 50-51 1.3847812930372996 1101 52-53 1.362690248808974 1101 54-55 1.3666384958433042 1101 56-57 1.330771706044338 1101 58-59 1.3875915810698842 1101 60-61 1.364094184096885 1101 62-63 1.3304973970751668 1101 64-65 1.3271511340182336 1101 66-67 1.4280302202908217 1101 68-69 1.412096772847164 1101 70-71 1.2934808410205463 1101 72-73 1.3344514404248145 1101 74-75 1.3694430115047957 1101 76-77 1.4221171752575934 1101 78-79 1.2886891255186725 1101 80-81 1.0950067298979427 1101 82-83 1.2955536179897287 1101 84-85 1.3425228932629594 1101 86-87 1.318217229080279 1101 88-89 1.3529927243279474 1101 90-91 1.27729105407456 1101 92-93 1.2564629945111427 1101 94-95 1.1705533079787784 1101 96-97 1.332397727845855 1101 98-99 1.3227319161762878 1101 100-101 1.3446002023874541 1101 102-103 1.2252342177179258 1101 104-105 1.3050962402185249 1101 106-107 1.2132963678765236 1101 108-109 1.2136979927666864 1101 110-111 1.2222887471186716 1101 112-113 1.2334771533362883 1101 114-115 1.3259437808886219 1101 116-117 1.376403010967973 1101 118-119 1.337520617720351 1101 120-121 1.2890811166897258 1101 122-123 1.3086853350791152 1101 124-125 1.5296467893450831 1102 1 0.1231666969119658 1102 2 -0.583169472596925 1102 3 -0.007522980236885246 1102 4 0.0720165589063555 1102 5 0.29857614453082704 1102 6 0.11310345015773748 1102 7 0.16648788019973892 1102 8 0.23161814255701074 1102 9 0.6178825204505074 1102 10-11 0.582013853061909 1102 12-13 0.6801056981509888 1102 14-15 0.8389414321756377 1102 16-17 0.8727106491534116 1102 18-19 0.881136643752356 1102 20-21 0.647038482075331 1102 22-23 0.8143587354534603 1102 24-25 0.8153931740998814 1102 26-27 0.9160199730955085 1102 28-29 0.5391210891572555 1102 30-31 0.7046994875650867 1102 32-33 0.9058803884564668 1102 34-35 0.8696486348894652 1102 36-37 0.5565968968996913 1102 38-39 -0.11022983199949721 1102 40-41 -0.42618250699461413 1102 42-43 -0.03822451976313346 1102 44-45 -0.4411565467783305 1102 46-47 -0.3083907609079759 1102 48-49 -0.46069300242687206 1102 50-51 -0.5170719533623256 1102 52-53 -0.7212401200174767 1102 54-55 -0.5528185335509335 1102 56-57 -0.6266366726413395 1102 58-59 -0.5346424127917402 1102 60-61 -0.730204088374883 1102 62-63 -0.5542254319652251 1102 64-65 -0.5744751489393529 1102 66-67 -0.7082220041289204 1102 68-69 -0.5021355759122343 1102 70-71 -1.0469530717821982 1102 72-73 -0.9473347273300412 1102 74-75 -0.7719223165415734 1102 76-77 -1.0850218663564277 1102 78-79 -0.8537032521553343 1102 80-81 -0.810313093811807 1102 82-83 -1.081251658128835 1102 84-85 -1.0316490016920206 1102 86-87 -0.7561414060544998 1102 88-89 -0.9024662181811998 1102 90-91 -0.9752042621120367 1102 92-93 -0.7435260400394235 1102 94-95 -0.5086360918209394 1102 96-97 -0.8301687626520895 1102 98-99 -0.9284785555146229 1102 100-101 -0.742870867339132 1102 102-103 -0.7838357754578666 1102 104-105 -0.6957467821099783 1102 106-107 -0.48797627221635054 1102 108-109 -0.5674157935659814 1102 110-111 -0.5400335342758389 1102 112-113 -0.35263512631513905 1102 114-115 -0.36719342137806166 1102 116-117 -0.47895623139637067 1102 118-119 -0.1349106047022559 1102 120-121 0.1338767506180183 1102 122-123 0.08431956528177764 1102 124-125 0.1654263601716366 1103 1 0.38224080160144425 1103 2 -0.06853894139645078 1103 3 0.291885989400658 1103 4 0.24344871369445542 1103 5 0.3415363700544418 1103 6 0.36004155087946543 1103 7 0.5084285177771335 1103 8 0.5990980757436404 1103 9 0.8501450536576627 1103 10-11 0.8265971673925776 1103 12-13 0.7442226585988507 1103 14-15 0.8210138437277408 1103 16-17 0.7792712714526573 1103 18-19 0.7681716723133576 1103 20-21 0.8955073186562217 1103 22-23 0.9158844831132882 1103 24-25 0.8526637169299889 1103 26-27 0.8765343847160167 1103 28-29 0.9191251794807158 1103 30-31 0.9544104570430108 1103 32-33 0.9794690130816406 1103 34-35 0.9428321464677865 1103 36-37 1.0221363492809132 1103 38-39 1.0650971800976023 1103 40-41 1.032368335583513 1103 42-43 1.0387294291805063 1103 44-45 1.0790996043840622 1103 46-47 1.0588427552309838 1103 48-49 1.0513271327960823 1103 50-51 1.0154459756513177 1103 52-53 1.0447401466659159 1103 54-55 0.9985360476113456 1103 56-57 1.0089379536712713 1103 58-59 1.0876785678597471 1103 60-61 1.027813301913227 1103 62-63 0.9479493516894486 1103 64-65 0.950499696283952 1103 66-67 0.877192572244688 1103 68-69 1.062841065062834 1103 70-71 0.9438863138996751 1103 72-73 0.8327081284283366 1103 74-75 0.894484512445846 1103 76-77 0.9983991405187354 1103 78-79 0.9297945729897528 1103 80-81 0.9437286303379189 1103 82-83 1.078558633524061 1103 84-85 1.0333669035378925 1103 86-87 1.1091462847797544 1103 88-89 1.0948546912102977 1103 90-91 1.0397542788254022 1103 92-93 0.9654262100660382 1103 94-95 0.9836712496683333 1103 96-97 0.9394211788577103 1103 98-99 0.9800715032036109 1103 100-101 0.88608755388152 1103 102-103 0.6994386496165319 1103 104-105 0.7628777432984819 1103 106-107 0.8553307756817645 1103 108-109 0.9199712593488378 1103 110-111 0.8524030015764872 1103 112-113 0.7306289660606105 1103 114-115 0.8587323171604773 1103 116-117 0.8758397084944036 1103 118-119 0.7309391849382507 1103 120-121 0.7514994175659275 1103 122-123 0.7823906998548473 1103 124-125 0.967272492749423 1104 1 0.4037886546823799 1104 2 0.5920333616770321 1104 3 0.3841553659738217 1104 4 0.1836252005739638 1104 5 0.384464680425296 1104 6 0.4044387933400202 1104 7 0.5464541048362079 1104 8 0.41816500071698925 1104 9 0.472319552201796 1104 10-11 0.4949540552190257 1104 12-13 0.5427121157261254 1104 14-15 0.6533167743108805 1104 16-17 0.5330776884274115 1104 18-19 0.46541753522164697 1104 20-21 0.34750409186418096 1104 22-23 0.37781340658823837 1104 24-25 0.6145210208642595 1104 26-27 0.5524684158040074 1104 28-29 0.7563149784084331 1104 30-31 0.5935056759878563 1104 32-33 0.6308845282217703 1104 34-35 0.8198248878868881 1104 36-37 0.7928755309876863 1104 38-39 0.8772853969309438 1104 40-41 0.8728583833593646 1104 42-43 0.8739643718950845 1104 44-45 0.7978103029484771 1104 46-47 0.8503352384114464 1104 48-49 0.8245934465706526 1104 50-51 0.7429792829906674 1104 52-53 0.90942776638623 1104 54-55 0.5832425708075704 1104 56-57 0.7140566583816934 1104 58-59 0.8139374556576158 1104 60-61 0.8557389686554302 1104 62-63 0.8462333066667789 1104 64-65 0.8554110615735446 1104 66-67 0.8654233892654375 1104 68-69 0.6621703931456544 1104 70-71 0.27426341034482604 1104 72-73 0.17514360789819605 1104 74-75 0.6355245166375489 1104 76-77 0.7702937274424002 1104 78-79 0.7016649351566713 1104 80-81 0.7294503477642991 1104 82-83 0.7914152426977559 1104 84-85 0.7303927362468912 1104 86-87 0.7829437399554493 1104 88-89 0.7607917571975804 1104 90-91 0.7315577030900329 1104 92-93 0.7751132888121504 1104 94-95 0.8022562993188807 1104 96-97 0.8627948028609076 1104 98-99 0.8716032155196558 1104 100-101 0.8098867782810224 1104 102-103 0.679859194115572 1104 104-105 0.6818912513222557 1104 106-107 0.7431402452480533 1104 108-109 0.7393598428197983 1104 110-111 0.7368096073138055 1104 112-113 0.6394048945458621 1104 114-115 0.6329700708994963 1104 116-117 0.6981065809354092 1104 118-119 0.7429439128486379 1104 120-121 0.6779484288760287 1104 122-123 0.7306585617002419 1104 124-125 1.137767215358945 1105 1 0.11260376857506316 1105 2 0.0025806432885033814 1105 3 0.21453864417646074 1105 4 0.23753551594884925 1105 5 -0.20818494845292435 1105 6 0.1575314861575947 1105 7 0.27238966432530987 1105 8 0.3214100106300961 1105 9 0.48955485199729054 1105 10-11 0.4516820254835565 1105 12-13 0.4545223538619041 1105 14-15 0.5517270515024961 1105 16-17 0.5599494111003764 1105 18-19 0.5095103192867612 1105 20-21 0.4688330104131495 1105 22-23 0.45309813576196944 1105 24-25 0.41614556519307655 1105 26-27 0.526226801665544 1105 28-29 0.5813860131602127 1105 30-31 0.6276999964409455 1105 32-33 0.6228293121670205 1105 34-35 0.6549854378910425 1105 36-37 0.6350540581090698 1105 38-39 0.7584501267350916 1105 40-41 0.5090873006299077 1105 42-43 0.5493823130003079 1105 44-45 0.6363254343127949 1105 46-47 0.6221145335543383 1105 48-49 0.6504901396152292 1105 50-51 0.4272231402356965 1105 52-53 0.49457690199339055 1105 54-55 0.5205573066892484 1105 56-57 0.34136651803297724 1105 58-59 0.2623503674611527 1105 60-61 0.3572696815678924 1105 62-63 0.565611185166329 1105 64-65 0.3260264805400901 1105 66-67 0.44175665340215886 1105 68-69 0.42991727775932276 1105 70-71 0.5421996555770754 1105 72-73 0.537845998685075 1105 74-75 0.4550318191798084 1105 76-77 0.46046837008384145 1105 78-79 0.43180900818283874 1105 80-81 0.4516900438782123 1105 82-83 0.43222770831505386 1105 84-85 0.4288927187617304 1105 86-87 0.6499790020208742 1105 88-89 0.5723346515545131 1105 90-91 0.32935585069066775 1105 92-93 0.4637834970195449 1105 94-95 0.3956303619263224 1105 96-97 0.5033470878676134 1105 98-99 0.4138546459718846 1105 100-101 0.4231164127477278 1105 102-103 0.15022258319648074 1105 104-105 0.2829867899265537 1105 106-107 0.31063734565301004 1105 108-109 0.32330676340488296 1105 110-111 0.26772936555721927 1105 112-113 0.1419182940333208 1105 114-115 0.1989776178620417 1105 116-117 -0.10935276639376568 1105 118-119 0.12159690036666859 1105 120-121 0.19848142405912128 1105 122-123 0.24874667759827318 1105 124-125 0.706208007376727 1106 1 -0.22473048466647327 1106 2 -0.6005652289364143 1106 3 -0.10517224403639247 1106 4 -0.19559684618091921 1106 5 -0.01829556839653179 1106 6 0.27436704671825396 1106 7 0.26474223634573946 1106 8 0.2756354397227909 1106 9 0.3073409988349596 1106 10-11 0.20845346980408408 1106 12-13 0.2030263094009186 1106 14-15 0.14978924755876477 1106 16-17 0.1565290124809593 1106 18-19 0.28225020537375656 1106 20-21 0.3242334264552653 1106 22-23 0.15694921535310158 1106 24-25 0.25373133798622405 1106 26-27 0.29608881063787607 1106 28-29 0.3646801941971347 1106 30-31 0.3653479021020587 1106 32-33 0.26819370060340475 1106 34-35 0.3931731853799434 1106 36-37 0.3467986025163583 1106 38-39 0.3369766895641604 1106 40-41 0.30145455261980914 1106 42-43 0.24968614450661164 1106 44-45 0.24054300598952238 1106 46-47 0.20039331193142118 1106 48-49 0.40486810469320744 1106 50-51 0.427119448387856 1106 52-53 0.1831770901464651 1106 54-55 0.21275333348902592 1106 56-57 -0.2163342259234824 1106 58-59 0.24929612579637705 1106 60-61 0.2505948598195218 1106 62-63 0.20358826618392456 1106 64-65 0.23724264884737423 1106 66-67 0.09909114514179862 1106 68-69 0.2812217972778228 1106 70-71 0.3872169744458205 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-1.363793890661185 2216 68-69 -1.2402844453978865 2216 70-71 -1.3143747878917011 2216 72-73 -1.2144568213617788 2216 74-75 -1.2250410151274558 2216 76-77 -1.3158161047227992 2216 78-79 -1.2628752111064294 2216 80-81 -1.1777879857256153 2216 82-83 -1.0354043306872995 2216 84-85 -1.1146807705762072 2216 86-87 -1.3067302427131757 2216 88-89 -1.1913311722364384 2216 90-91 -1.17249042599542 2216 92-93 -1.1686592776816056 2216 94-95 -1.1349858140708662 2216 96-97 -1.1498485803474345 2216 98-99 -1.2331742880072625 2216 100-101 -1.3111287336721809 2216 102-103 -1.174019337683852 2216 104-105 -1.1372337478042667 2216 106-107 -1.2258709169087432 2216 108-109 -1.2463888283920674 2216 110-111 -1.2321411553822408 2216 112-113 -1.14994980240348 2216 114-115 -1.2181739214149232 2216 116-117 -1.2338913905226612 2216 118-119 -1.2410673437733024 2216 120-121 -1.4034551303635787 2216 122-123 -1.356148468525241 2216 124-125 -1.6588881814865424 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 76048.0 3 26054.0 4 12827.0 5 10214.0 6 10901.0 7 13083.0 8 14443.0 9 15723.0 10 17121.0 11 18397.0 12 20200.0 13 22855.0 14 26085.0 15 22789.0 16 16135.0 17 14409.0 18 13222.0 19 12923.0 20 12615.0 21 13099.0 22 15048.0 23 18639.0 24 24041.0 25 31670.0 26 38730.0 27 44406.0 28 51313.0 29 60387.0 30 70394.0 31 82203.0 32 101178.0 33 124721.0 34 161137.0 35 223238.0 36 362290.0 37 522364.0 38 177469.0 39 541.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7155672252498 19.11956563101468 14.205315852825661 23.959551290909857 2 15.706808168361405 20.530111128452006 38.895638498385296 24.867442204801293 3 19.41428909861572 24.941694625501018 29.10222528844553 26.541790987437736 4 12.991344871635157 16.74993861255973 36.09982058444048 34.15889593136463 5 13.192701463676993 38.60892260311687 33.84164788515962 14.356728048046511 6 32.35596131436401 36.84207367046138 16.77802179508522 14.023943220089382 7 28.69532472116363 32.58196614705785 20.401846116660067 18.32086301511845 8 26.791344955939987 35.34365769138261 18.906645760221807 18.958351592455593 9 26.557527021786708 16.01573864752801 19.300893182654033 38.12584114803125 10-11 25.334302293590554 26.735879828652504 26.676873830609765 21.252944047147174 12-13 26.30106622402069 23.77874851135214 27.438901409893585 22.48128385473358 14-15 23.04192166946355 25.15696340465175 25.312631244696437 26.488483681188264 16-17 22.48261839380913 28.510676271936596 26.08443454855845 22.92227078569582 18-19 22.550260959206163 26.63502688027891 28.63476035853178 22.179951801983147 20-21 23.15852428228818 25.72115336516894 28.955103468395226 22.165218884147652 22-23 22.87787645183184 25.590877149735565 28.96226437745707 22.56898202097553 24-25 22.614661900859254 25.834383123535364 28.983033416142707 22.56792155946268 26-27 22.218789617241423 26.24114014419075 28.81836175103405 22.721708487533775 28-29 22.428520892292326 26.199401979741584 28.907220422327796 22.464856705638294 30-31 22.436364305745858 25.932345756873392 28.847774551484807 22.783515385895942 32-33 22.33908196847268 26.005997810247017 28.749211656913086 22.905708564367213 34-35 22.75284203685444 26.101519381234713 28.60526901307449 22.540369568836358 36-37 22.907569374191645 25.662128158174436 28.53823984197923 22.892062625654685 38-39 22.623825889026904 26.01984383603744 28.55228595484755 22.804044320088103 40-41 22.754542777016557 25.921721133037096 28.63550217054462 22.688233919401725 42-43 22.627207360643354 25.784561441139182 28.340613835141053 23.24761736307641 44-45 22.687473588505718 26.056279692922356 28.399359401211406 22.856887317360517 46-47 23.200997073926573 26.29746465661856 27.63228557068036 22.869252698774506 48-49 23.053732984594895 26.450131097053436 27.338757827406486 23.15737809094518 50-51 23.15224511797339 26.602568078655064 27.30413368063128 22.941053122740264 52-53 23.34497973518075 26.30712886248095 27.29439852223688 23.05349288010142 54-55 23.159138857230666 26.492749644645347 27.260063579669875 23.08804791845411 56-57 23.064562862786907 26.753163229695943 27.059340972469016 23.122932935048137 58-59 22.952272447148577 26.31094100905521 27.34089748576569 23.39588905803052 60-61 22.922796000819556 26.54505240680744 27.44098231550371 23.091169276869294 62-63 22.91975467723553 26.49308979267777 27.686669238452577 22.90048629163412 64-65 22.773051823646934 26.419365262619763 27.522947528069768 23.284635385663535 66-67 22.777332695188946 26.664904566467328 27.38065606151797 23.177106676825755 68-69 23.00107466791041 26.387589156318665 27.53468860341793 23.076647572353 70-71 22.979280582909684 26.329918780653337 27.429817456557092 23.260983179879887 72-73 22.881858184681974 26.463316835486804 27.218945685162183 23.43587929466904 74-75 22.78787073579305 26.879040306825214 26.99471203522215 23.33837692215959 76-77 23.096391549602387 26.504314677747754 27.271228438616486 23.12806533403337 78-79 23.060836075860212 26.54239124867142 27.18297002855643 23.213802646911937 80-81 23.06067445406197 26.783017395584473 26.936664385672156 23.219643764681404 82-83 23.08326583729239 26.733354355815653 27.03794691449719 23.145432892394773 84-85 22.877896460539628 26.856147795520613 26.998169603411405 23.267786140528358 86-87 23.093270191187205 26.742298248197617 26.97986163578389 23.184569924831287 88-89 22.963426280806495 26.7756472938225 26.887157049475473 23.373769375895527 90-91 22.827479084393346 26.9393293840138 26.949693896722632 23.283497634870223 92-93 22.891829517528187 26.68093583436614 27.023651975840192 23.403582672265486 94-95 22.744898579861957 26.48616677978256 27.145773840775504 23.623160799579974 96-97 23.044367595378866 26.39976179546667 27.05562468058301 23.500245928571456 98-99 22.788601047259124 26.58509045411043 26.853778510838268 23.772529987792183 100-101 22.63921856838517 26.4427489530682 27.14745325798933 23.770579220557302 102-103 22.599395256815765 26.088153564431238 27.30556338118349 24.0068877975695 104-105 22.437044601810708 26.45589360489685 27.102555031949905 24.004506761342537 106-107 22.661009179543644 26.34565242750578 26.92401349969376 24.069324893256816 108-109 22.654148146932805 26.296794883851405 26.8024751581789 24.246581811036886 110-111 22.588771293055064 26.31413927886486 27.07759290810084 24.01949651997923 112-113 22.579946792844247 26.426900987309676 27.100954335326733 23.89219788451934 114-115 22.406611357262683 26.659622267610867 26.844062536015674 24.089703839110783 116-117 22.316812276702823 26.56121944270146 27.118001754363497 24.003966526232215 118-119 22.211726543391684 26.617483929005903 26.88478025636757 24.28600927123484 120-121 22.11528572520486 26.60778578623203 27.036816790835278 24.24011169772783 122-123 22.041719700551727 26.65318150284807 26.972995589274912 24.33210320732529 124-125 21.96462593534687 26.704350887395513 26.868480908170095 24.462542269087525 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 300.0 1 263.0 2 429.5 3 659.0 4 472.5 5 311.5 6 662.0 7 1640.5 8 2766.0 9 3209.0 10 2480.5 11 1571.5 12 1386.0 13 1389.0 14 1488.0 15 1697.5 16 1896.5 17 2111.0 18 2391.5 19 2756.5 20 3194.0 21 3648.0 22 4256.0 23 5370.5 24 6697.5 25 8354.0 26 11015.0 27 14378.5 28 18075.5 29 22188.5 30 26593.0 31 32516.0 32 40063.5 33 44813.5 34 47291.0 35 51095.0 36 56955.5 37 62522.5 38 65772.0 39 68126.0 40 69910.5 41 71755.5 42 72048.5 43 74011.5 44 78636.0 45 81967.5 46 83488.0 47 85515.5 48 87844.5 49 88346.5 50 88670.5 51 87591.0 52 88363.5 53 92307.5 54 93618.5 55 89750.5 56 84139.0 57 78412.5 58 72930.0 59 65977.5 60 54924.5 61 44418.5 62 36061.5 63 27069.0 64 19173.5 65 14370.0 66 10984.5 67 8480.5 68 6662.0 69 5175.0 70 3839.0 71 2627.0 72 1918.5 73 1597.5 74 1239.0 75 891.0 76 595.5 77 345.0 78 233.0 79 139.0 80 87.0 81 49.0 82 25.5 83 19.5 84 12.5 85 9.0 86 9.0 87 4.5 88 4.5 89 4.0 90 1.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09672209345507166 2 8.003483115852018E-5 3 0.0 4 0.0989630687275102 5 0.0 6 0.0 7 0.0018007837010667043 8 0.006442803908260875 9 0.003801654480029709 10-11 5.402351103200113E-4 12-13 0.0 14-15 6.002612336889014E-5 16-17 2.4010449347556056E-4 18-19 0.002160940441280045 20-21 0.0013205747141155832 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 4.001741557926009E-5 52-53 0.0 54-55 0.0 56-57 0.008003483115852018 58-59 8.003483115852019E-4 60-61 0.0 62-63 0.0 64-65 1.8007837010667043E-4 66-67 0.0 68-69 2.0008707789630046E-5 70-71 0.0 72-73 0.0 74-75 0.0012005224673778028 76-77 0.0 78-79 0.0 80-81 8.003483115852018E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0011004789284296526 90-91 2.0008707789630046E-5 92-93 0.002040888194542265 94-95 0.0 96-97 0.00876381401185796 98-99 0.00414180251245342 100-101 0.0025010884737037557 102-103 0.0 104-105 0.0 106-107 0.0016207053309600337 108-109 4.001741557926009E-5 110-111 1.8007837010667043E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0010004353894815022 122-123 0.004662028914983801 124-125 0.08447676428781806 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2498912.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.29422077850627 #Duplication Level Percentage of deduplicated Percentage of total 1 74.76498475636573 37.602466498383144 2 12.662055933419127 12.736564732503542 3 4.881282177884069 7.365008506100679 4 2.3980297018686385 4.824281410367876 5 1.3314137517155749 3.3481208588161233 6 0.868023590540755 2.6193942062165076 7 0.602332286398412 2.1205683095900945 8 0.4022405026855507 1.6184298118499547 9 0.29919937976979133 1.3543199696640629 >10 1.6089921584068423 14.679719671207621 >50 0.12040268158663738 4.140091929809355 >100 0.05656953656756195 4.96974104964815 >500 0.0030356183227654714 1.0339247925718624 >1k 0.001278155083267763 1.0322373424545777 >5k 1.5976938540847036E-4 0.5551309108163981 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7411 0.2965690668578966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6406 0.2563515642007402 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4186 0.16751290161478274 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.2005224673778028E-4 14-15 0.0 0.0 0.0 4.001741557926009E-5 1.8007837010667043E-4 16-17 0.0 0.0 0.0 6.0026123368890134E-5 2.4010449347556056E-4 18-19 0.0 0.0 0.0 8.003483115852018E-5 2.4010449347556056E-4 20-21 0.0 0.0 0.0 1.6006966231704037E-4 2.8012190905482066E-4 22-23 0.0 0.0 0.0 3.6015674021334086E-4 3.2013932463408073E-4 24-25 0.0 0.0 0.0 6.402786492681616E-4 3.2013932463408073E-4 26-27 0.0 0.0 0.0 9.003918505333521E-4 3.401480324237108E-4 28-29 0.0 0.0 0.0 0.0011605050517985427 3.6015674021334086E-4 30-31 0.0 0.0 0.0 0.0027812103827585767 3.6015674021334086E-4 32-33 0.0 0.0 0.0 0.006502830031629766 3.6015674021334086E-4 34-35 0.0 0.0 0.0 0.011024797992086156 3.6015674021334086E-4 36-37 0.0 0.0 0.0 0.018247941504142603 3.6015674021334086E-4 38-39 0.0 0.0 0.0 0.031933897632249554 4.2018286358223096E-4 40-41 0.0 0.0 0.0 0.04900132537680398 4.4019157137186105E-4 42-43 0.0 0.0 0.0 0.06754939749779104 4.4019157137186105E-4 44-45 0.0 0.0 0.0 0.08851852326132333 4.602002791614911E-4 46-47 0.0 0.0 0.0 0.11432975630994609 4.802089869511211E-4 48-49 0.0 0.0 0.0 0.1419617817674252 4.802089869511211E-4 50-51 0.0 0.0 0.0 0.1767369159058022 4.802089869511211E-4 52-53 0.0 0.0 0.0 0.21099182364164884 5.002176947407512E-4 54-55 0.0 0.0 0.0 0.24622715805918735 5.202264025303812E-4 56-57 0.0 0.0 0.0 0.291026654800169 5.602438181096413E-4 58-59 0.0 0.0 0.0 0.3403081020860278 5.602438181096413E-4 60-61 0.0 0.0 0.0 0.3977130847344764 5.602438181096413E-4 62-63 0.0 0.0 0.0 0.45579836344777247 6.402786492681615E-4 64-65 0.0 0.0 0.0 0.5149040862583396 6.402786492681615E-4 66-67 0.0 0.0 0.0 0.5864952427296359 6.602873570577916E-4 68-69 0.0 0.0 0.0 0.6725727036406244 6.802960648474216E-4 70-71 0.0 0.0 0.0 0.7685544749074797 7.003047726370517E-4 72-73 0.0 0.0 0.0 0.8689981880114226 8.003483115852019E-4 74-75 0.0 0.0 0.0 0.9688216311738869 8.403657271644619E-4 76-77 0.0 0.0 0.0 1.0819108476008759 8.403657271644619E-4 78-79 0.0 0.0 0.0 1.2188104262975248 8.60374434954092E-4 80-81 0.0 0.0 0.0 1.3743181032385294 9.003918505333521E-4 82-83 0.0 0.0 0.0 1.5381293939122307 0.0011204876362192826 84-85 0.0 0.0 0.0 1.7027610416053065 0.0011204876362192826 86-87 0.0 0.0 0.0 1.8760564597712923 0.0011404963440089127 88-89 0.0 0.0 0.0 2.0764436682844374 0.0011605050517985427 90-91 0.0 0.0 0.0 2.3068239297742377 0.0011605050517985427 92-93 0.0 0.0 0.0 2.554611767041016 0.001280557298536323 94-95 0.0 0.0 0.0 2.801719308242947 0.001280557298536323 96-97 0.0 0.0 0.0 3.057290532839892 0.0013806008374844733 98-99 0.0 0.0 0.0 3.3365520674597584 0.0015206617920118836 100-101 0.0 0.0 0.0 3.673698793715025 0.0015206617920118836 102-103 0.0 0.0 0.0 4.029013426643274 0.0015206617920118836 104-105 0.0 0.0 0.0 4.389830454213674 0.0015406704998015136 106-107 0.0 0.0 0.0 4.746245566070353 0.0015606792075911436 108-109 0.0 0.0 0.0 5.130272694676723 0.0015806879153807737 110-111 0.0 0.0 0.0 5.567342907633401 0.0016407140387496638 112-113 0.0 0.0 0.0 6.034106042949892 0.0016407140387496638 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4655 0.0 65.823586 1 GTATCAA 8425 0.0 54.128952 1 ATCAACG 11095 0.0 40.958466 3 TCAACGC 11265 0.0 40.551846 4 CAACGCA 11350 0.0 39.996593 5 TATCAAC 11465 0.0 39.117165 2 AACGCAG 11915 0.0 38.099983 6 GTACATG 11635 0.0 33.610542 1 TACATGG 11540 0.0 32.876083 2 ACGCAGA 14170 0.0 31.82685 7 CGCAGAG 14340 0.0 31.366566 8 ACATGGG 12000 0.0 30.72245 3 GCAGAGT 15965 0.0 27.428576 9 GTGGTAT 2460 0.0 26.656042 1 GAGTACT 9430 0.0 25.237286 12-13 CATGGGG 5935 0.0 25.188334 4 TGGTATC 2470 0.0 25.07742 2 TAAGGTG 1390 0.0 24.826044 5 CAGAGTA 14975 0.0 24.433718 10-11 AGAGTAC 14600 0.0 24.409296 10-11 >>END_MODULE