FastQCFastQC Report
Thu 26 May 2016
SRR1364537_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364537_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676243
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38870.23188762011235842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27350.16316250090231546No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20560.12265524747903495No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77200.048.399211
GGTATCA47250.039.790671
GTACATG123350.038.5875131
TATCAAC98600.037.713692
ATCAACG96800.037.554483
TCAACGC96750.037.3279084
CAACGCA99350.036.4109235
AACGCAG99350.036.3477826
TACATGG129200.036.1956182
ACATGGG132600.034.504743
CATGGGG50200.032.0004464
ACGCAGA114450.031.34437
CGCAGAG114600.030.6858168
CATGGGA82050.028.2801674
GCAGAGT119450.027.995299
ATGGGGA29950.026.81845
ATGGGAG31950.026.6293835
GAGTACT39500.026.4403212-13
AGTACTT40500.024.97931912-13
CATGGGT16500.023.438264
Produced by FastQC (version 0.11.5)