##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364537_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1676243 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.326477724291763 31.0 27.0 31.0 10.0 34.0 2 27.36541658936085 31.0 27.0 33.0 10.0 34.0 3 27.45534985082712 31.0 27.0 34.0 10.0 34.0 4 30.622426462034444 35.0 30.0 37.0 10.0 37.0 5 30.828157373364125 35.0 31.0 37.0 10.0 37.0 6 30.67009138889767 35.0 31.0 37.0 8.0 37.0 7 30.49071882775946 35.0 31.0 37.0 8.0 37.0 8 30.39194734892256 35.0 30.0 37.0 8.0 37.0 9 31.27739235898375 37.0 30.0 39.0 8.0 39.0 10-11 31.24050063147169 37.0 30.0 39.0 7.0 39.0 12-13 31.122509982144592 37.0 30.0 39.0 4.5 39.0 14-15 31.741252909035268 38.0 30.0 40.0 2.0 41.0 16-17 31.86213245931527 38.0 30.0 40.0 2.0 41.0 18-19 31.672293038658474 38.0 30.0 40.0 2.0 41.0 20-21 31.41520352359413 37.5 28.5 40.0 2.0 41.0 22-23 31.299427052044365 37.0 28.5 40.0 2.0 41.0 24-25 31.14034808795622 37.0 28.0 40.0 2.0 41.0 26-27 31.035821775243804 37.0 28.0 40.0 2.0 41.0 28-29 31.075566012803634 37.0 28.5 40.0 2.0 41.0 30-31 31.004253261609442 37.0 28.5 40.0 2.0 41.0 32-33 30.95518728489843 37.0 29.0 40.0 2.0 41.0 34-35 30.495523023809795 36.0 27.5 40.0 2.0 41.0 36-37 30.31265574263397 36.0 27.5 40.0 2.0 41.0 38-39 30.094485107469502 36.0 27.0 40.0 2.0 41.0 40-41 30.240239631127466 36.0 27.5 40.0 2.0 41.0 42-43 30.076864153944264 36.0 27.0 40.0 2.0 41.0 44-45 29.870042112032685 35.5 27.0 40.0 2.0 41.0 46-47 29.74051882692426 35.0 27.0 40.0 2.0 41.0 48-49 29.510757091901354 35.0 26.5 40.0 2.0 41.0 50-51 29.306684054758172 35.0 26.0 40.0 2.0 41.0 52-53 29.199287036545417 35.0 26.0 39.5 2.0 41.0 54-55 29.194245703039478 35.0 26.0 40.0 2.0 41.0 56-57 29.049792005097114 35.0 26.0 39.0 2.0 41.0 58-59 28.820834151134413 35.0 26.0 39.0 2.0 41.0 60-61 28.591247510056718 34.5 26.0 39.0 2.0 41.0 62-63 28.298362767212154 34.0 25.0 39.0 2.0 40.0 64-65 28.014234511344714 34.0 24.5 38.0 2.0 40.0 66-67 27.764518628862284 34.0 24.0 38.0 2.0 40.0 68-69 27.744733311339704 34.0 25.0 37.0 2.0 40.0 70-71 27.51979724896689 34.0 24.0 37.0 2.0 40.0 72-73 27.24727679697991 34.0 24.0 36.5 2.0 39.0 74-75 26.96307247815502 34.0 23.5 36.0 2.0 39.0 76-77 26.67351153740836 33.5 23.0 35.5 2.0 38.5 78-79 26.37706973272968 33.0 22.0 35.0 2.0 37.0 80-81 26.09124064947624 33.0 20.5 35.0 2.0 37.0 82-83 25.860607620732793 33.0 20.0 35.0 2.0 36.5 84-85 25.635743445311928 33.0 20.0 35.0 2.0 36.0 86-87 25.442680148403305 33.0 18.5 35.0 2.0 36.0 88-89 25.256657895066525 33.0 18.0 35.0 2.0 35.5 90-91 25.1230886571935 33.0 17.5 35.0 2.0 35.0 92-93 25.001249222218974 33.0 16.0 35.0 2.0 35.0 94-95 24.874389333766047 33.0 12.0 35.0 2.0 35.0 96-97 24.759760965444748 33.0 9.0 35.0 2.0 35.0 98-99 24.65846538956464 32.0 7.0 35.0 2.0 35.0 100-101 24.55235607247875 32.0 7.0 35.0 2.0 35.0 102-103 23.4526661707163 30.5 3.5 34.0 2.0 35.0 104-105 24.258894742588037 32.0 2.0 35.0 2.0 35.0 106-107 24.399400623895225 32.0 2.0 35.0 2.0 35.0 108-109 24.331253583161867 32.0 2.0 35.0 2.0 35.0 110-111 24.24169765362182 32.0 2.0 35.0 2.0 35.0 112-113 24.136325103221907 32.0 2.0 35.0 2.0 35.0 114-115 24.00481016177249 32.0 2.0 35.0 2.0 35.0 116-117 23.876433190175888 32.0 2.0 35.0 2.0 35.0 118-119 23.707310037983753 32.0 2.0 35.0 2.0 35.0 120-121 23.519018423939727 32.0 2.0 35.0 2.0 35.0 122-123 23.250340791878028 32.0 2.0 35.0 2.0 35.0 124-125 21.81474523681829 29.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8940857095290902 1101 2 0.93329213285433 1101 3 0.8492187917203076 1101 4 0.9003611099775419 1101 5 0.8703754594380122 1101 6 0.7983083917339862 1101 7 1.014180939566515 1101 8 0.8849275956187235 1101 9 1.294949609996113 1101 10-11 1.244893879603861 1101 12-13 0.8921272812553873 1101 14-15 1.088290732737402 1101 16-17 0.9525891747691944 1101 18-19 1.3601163136964338 1101 20-21 1.6966848042665532 1101 22-23 1.5022572752657553 1101 24-25 1.6714527459238226 1101 26-27 1.9166916853578826 1101 28-29 1.64591056438098 1101 30-31 1.7604145696062368 1101 32-33 1.6783497807411543 1101 34-35 1.998745941120994 1101 36-37 1.899266110871494 1101 38-39 1.7085935013938354 1101 40-41 1.367179527108874 1101 42-43 1.4325163367446905 1101 44-45 1.3239567577697926 1101 46-47 1.1802134902189714 1101 48-49 1.0757680161149494 1101 50-51 0.947621811952505 1101 52-53 0.7975465868905225 1101 54-55 0.6332578518306562 1101 56-57 0.6883796291539426 1101 58-59 0.7419363928027707 1101 60-61 0.6848663028010549 1101 62-63 0.7219221336658528 1101 64-65 0.8573144226360441 1101 66-67 0.6722759061729562 1101 68-69 0.5525139618568424 1101 70-71 0.3860524914025376 1101 72-73 0.33731224044348096 1101 74-75 0.3292690706223631 1101 76-77 0.5286900829565866 1101 78-79 0.5376491665254512 1101 80-81 0.5577737268575724 1101 82-83 0.4049546003476685 1101 84-85 0.46625958501004305 1101 86-87 0.5270184419450636 1101 88-89 0.5895369948766991 1101 90-91 0.5474859173563971 1101 92-93 0.5065467599016316 1101 94-95 0.6051106537976416 1101 96-97 0.4861885853097334 1101 98-99 0.603672132381984 1101 100-101 0.6034364863927131 1101 102-103 0.5821573162285567 1101 104-105 0.6879356602486482 1101 106-107 0.601727796981443 1101 108-109 0.6182894441172486 1101 110-111 0.4420307329739295 1101 112-113 0.5243916991720852 1101 114-115 0.7309991826101445 1101 116-117 0.7588567154784762 1101 118-119 0.6463017742755675 1101 120-121 0.7735449856302594 1101 122-123 0.7720499179662461 1101 124-125 0.9836309435951094 1102 1 -0.6194575980220769 1102 2 -0.4729309861389268 1102 3 -0.2305722960275567 1102 4 -0.09485016219836595 1102 5 0.1063681318746923 1102 6 -0.8969924100501139 1102 7 -1.5321142750980492 1102 8 -0.5845256985398741 1102 9 -0.2824977784532372 1102 10-11 -0.2567817400236301 1102 12-13 -0.15574642742382494 1102 14-15 -0.03172555073664185 1102 16-17 -0.21929749579134494 1102 18-19 -0.08951199830908863 1102 20-21 0.34880408956363596 1102 22-23 0.19674102719056918 1102 24-25 0.2495881259010453 1102 26-27 -0.20367467805858297 1102 28-29 0.3439288921389938 1102 30-31 0.24635325878658065 1102 32-33 0.30365126100333484 1102 34-35 0.5541455204645871 1102 36-37 0.581731508489149 1102 38-39 0.6066062243328858 1102 40-41 0.4362567084159181 1102 42-43 0.23173925809432916 1102 44-45 0.24199284395421117 1102 46-47 -0.024761302845210054 1102 48-49 -0.384429288262119 1102 50-51 0.04968667150753703 1102 52-53 -0.25623319977236747 1102 54-55 -0.2720123004434285 1102 56-57 -0.2889868581585162 1102 58-59 -0.1976665205231285 1102 60-61 -0.2984996269794138 1102 62-63 -0.2686147194937867 1102 64-65 -0.206084855817263 1102 66-67 -0.18332050556294632 1102 68-69 -0.360748367269931 1102 70-71 -0.3513280374563834 1102 72-73 -0.18876122988342914 1102 74-75 -0.4292102092469001 1102 76-77 -0.22737735481517518 1102 78-79 -0.35230264678098067 1102 80-81 -0.787595291473135 1102 82-83 -0.6610240010435113 1102 84-85 -1.0454538966444957 1102 86-87 -0.4557805161485895 1102 88-89 -0.23353317745673507 1102 90-91 -0.7860966829256135 1102 92-93 -0.4271000008079966 1102 94-95 -0.3921051523818164 1102 96-97 -0.22217948315165614 1102 98-99 -0.15611078412981172 1102 100-101 -0.19236705290296996 1102 102-103 -0.4115008311132833 1102 104-105 -0.20044864743821478 1102 106-107 -0.1288052655506391 1102 108-109 -0.6023426319666925 1102 110-111 -0.5163826902725006 1102 112-113 -0.7690381255445757 1102 114-115 -0.698543080674753 1102 116-117 -0.8265773187522178 1102 118-119 -0.49088007850058446 1102 120-121 -0.03180274913790626 1102 122-123 -0.22397176678333608 1102 124-125 -0.015842424665095223 1103 1 0.34618173358913396 1103 2 0.2541013228539235 1103 3 0.13101257951109702 1103 4 0.22307869262765934 1103 5 0.22544454651609414 1103 6 0.16610084685777693 1103 7 0.3233990782449254 1103 8 0.4520128592931911 1103 9 0.5936009599967917 1103 10-11 0.6956282534079499 1103 12-13 0.6653510297415046 1103 14-15 0.8035158904288302 1103 16-17 0.5270975287003381 1103 18-19 0.6551039550109117 1103 20-21 1.1244244817251214 1103 22-23 1.131164949806866 1103 24-25 1.1727254766755593 1103 26-27 1.138717215385661 1103 28-29 1.1441031910406174 1103 30-31 0.9191544564195908 1103 32-33 1.0717279790293865 1103 34-35 1.4596537192139323 1103 36-37 1.2679998183420835 1103 38-39 1.395241449942084 1103 40-41 1.1320296470939262 1103 42-43 0.9718713776926826 1103 44-45 0.7734496352049511 1103 46-47 0.8407297973807069 1103 48-49 0.8190475587760524 1103 50-51 0.85484303242335 1103 52-53 0.8290103032305502 1103 54-55 0.6690907236830164 1103 56-57 0.6196096785436609 1103 58-59 0.4976362455790877 1103 60-61 0.49139265674120125 1103 62-63 0.5014619808593714 1103 64-65 0.5949138098793583 1103 66-67 0.6582581402811307 1103 68-69 0.7266695642358307 1103 70-71 0.7118482084088988 1103 72-73 0.8091979192257135 1103 74-75 0.7740655350027872 1103 76-77 0.78542967487234 1103 78-79 0.8471253406005737 1103 80-81 0.807677209800783 1103 82-83 0.8799434572705529 1103 84-85 0.8927608613988767 1103 86-87 0.7751471928849121 1103 88-89 0.7173334570059957 1103 90-91 0.7958712967387775 1103 92-93 0.8098962113020569 1103 94-95 0.8637113647034518 1103 96-97 0.9072407281572268 1103 98-99 0.7698120354034472 1103 100-101 0.7584853696087599 1103 102-103 0.7583788901262949 1103 104-105 0.7267405821120398 1103 106-107 0.7886925916111558 1103 108-109 0.9415591155878218 1103 110-111 0.9306293390762832 1103 112-113 1.039990881562872 1103 114-115 1.0641563652800734 1103 116-117 1.0936800132890312 1103 118-119 1.1704425754965833 1103 120-121 1.1639719568293465 1103 122-123 1.1488342172580417 1103 124-125 1.1266948283194118 1104 1 0.6098246328099144 1104 2 0.5889064063456999 1104 3 0.5262525293685734 1104 4 0.6044784955195084 1104 5 0.6647712518784985 1104 6 0.6969746557633805 1104 7 0.8214317658117558 1104 8 0.8736290065268371 1104 9 1.0616011777650023 1104 10-11 0.9448236277877946 1104 12-13 0.8901887505459065 1104 14-15 0.923405736008835 1104 16-17 0.8969610606215284 1104 18-19 1.2730183218498432 1104 20-21 1.4691148460401706 1104 22-23 1.0990393226762976 1104 24-25 1.558678868694635 1104 26-27 1.7702900462886433 1104 28-29 1.6122733657298163 1104 30-31 1.4815857407774153 1104 32-33 1.4925205157219388 1104 34-35 1.88964361253543 1104 36-37 1.846092196733892 1104 38-39 1.7555684791649 1104 40-41 1.5152263169154416 1104 42-43 1.4295353529676156 1104 44-45 1.2448307742443667 1104 46-47 1.1874209071330917 1104 48-49 1.0607741814982106 1104 50-51 0.9068667412393339 1104 52-53 1.033007396862505 1104 54-55 0.8903010682314125 1104 56-57 0.8177518085959505 1104 58-59 0.8246041163922797 1104 60-61 0.8948345112329505 1104 62-63 0.8597301931219583 1104 64-65 0.9605470547094583 1104 66-67 0.8676308980363281 1104 68-69 0.9232392961296618 1104 70-71 0.9010136117407512 1104 72-73 0.7314752582098514 1104 74-75 0.7433339036117736 1104 76-77 0.7592865313602957 1104 78-79 0.908396939438024 1104 80-81 0.8464229207441925 1104 82-83 0.8571124844887912 1104 84-85 0.7610001356165199 1104 86-87 0.7566420599765031 1104 88-89 0.8237716485247581 1104 90-91 0.862107522201466 1104 92-93 0.8363943449168119 1104 94-95 0.8781907870034757 1104 96-97 0.6489641401942272 1104 98-99 0.7362593285166668 1104 100-101 0.8695983885183018 1104 102-103 0.78372550452859 1104 104-105 0.8397664016045283 1104 106-107 0.831919930455701 1104 108-109 0.8728195328652149 1104 110-111 0.8651874092032301 1104 112-113 0.8478079925604476 1104 114-115 0.7773800606949344 1104 116-117 0.8102049212261235 1104 118-119 0.8305253134572119 1104 120-121 0.8223198778409042 1104 122-123 0.8097566889830716 1104 124-125 1.0630148666242825 1105 1 -0.23537482693259193 1105 2 0.014032927063265532 1105 3 5.436190275851516E-4 1105 4 0.13019209510230922 1105 5 0.09806089000402096 1105 6 0.04576912984714809 1105 7 0.2768915708387638 1105 8 -0.13761053770727827 1105 9 -0.1805823890002074 1105 10-11 0.14449800967822668 1105 12-13 0.11682002054179108 1105 14-15 0.2196345909030839 1105 16-17 0.14203057152650445 1105 18-19 0.24535456606555428 1105 20-21 0.598868440592689 1105 22-23 0.7243286169240903 1105 24-25 0.8799120398228268 1105 26-27 0.6872318506662616 1105 28-29 0.3999499671947042 1105 30-31 0.35982559833814065 1105 32-33 0.42311128203641246 1105 34-35 1.0269142038132983 1105 36-37 1.072567727062271 1105 38-39 1.1881815165360265 1105 40-41 0.8576731206642769 1105 42-43 0.3833015601596337 1105 44-45 0.542039467530298 1105 46-47 0.35237365591691017 1105 48-49 0.22938283371775015 1105 50-51 0.4289698377730602 1105 52-53 0.36091520636844976 1105 54-55 0.13002526976365658 1105 56-57 0.11951887121935556 1105 58-59 0.06934864862975232 1105 60-61 0.21682648694552142 1105 62-63 0.12978846050394566 1105 64-65 0.07346740793725459 1105 66-67 0.09631960840246379 1105 68-69 -0.09932000679323139 1105 70-71 0.019056322207781307 1105 72-73 0.07041862993102299 1105 74-75 0.09492821030382359 1105 76-77 0.15019018378232474 1105 78-79 0.14373037650307197 1105 80-81 0.07232664776779885 1105 82-83 -0.09801388702562619 1105 84-85 -0.0715535046916358 1105 86-87 -0.07106652780148437 1105 88-89 0.11855060406009343 1105 90-91 0.0073042820774738 1105 92-93 -0.09560532541527778 1105 94-95 -0.03755511861345795 1105 96-97 0.12246424001823897 1105 98-99 0.010317092870899103 1105 100-101 -0.001325825518588175 1105 102-103 -7.636913872239859E-4 1105 104-105 0.0584663874657565 1105 106-107 0.0728886993947313 1105 108-109 0.09832666370998311 1105 110-111 0.1869290078226058 1105 112-113 0.1415129696222408 1105 114-115 0.0973550940347323 1105 116-117 0.12624816740350653 1105 118-119 0.17496170566823466 1105 120-121 0.07587769796869637 1105 122-123 0.22991886570070363 1105 124-125 0.4132512115165987 1106 1 0.2603243069432537 1106 2 0.3034367684618928 1106 3 0.29443821398544756 1106 4 0.635797035722728 1106 5 0.3543570399428333 1106 6 0.34128894431884405 1106 7 0.00864563091941406 1106 8 0.5758618548875951 1106 9 0.6589613358979705 1106 10-11 0.7028637347836657 1106 12-13 0.6612764727057687 1106 14-15 1.0546791342007147 1106 16-17 0.7526193720813765 1106 18-19 0.8259587408335243 1106 20-21 1.2613911981364154 1106 22-23 1.1278367781262801 1106 24-25 1.3817260474620028 1106 26-27 1.5853229799255928 1106 28-29 1.3303272371044166 1106 30-31 1.1253685554783992 1106 32-33 1.1755150693490002 1106 34-35 1.2758954734808654 1106 36-37 1.339894863904206 1106 38-39 1.5503548987881572 1106 40-41 1.1467773635161613 1106 42-43 1.1050140120996446 1106 44-45 1.0828823420171219 1106 46-47 0.8327578661354202 1106 48-49 0.8819763325758601 1106 50-51 0.8349691814118572 1106 52-53 0.7006721240681166 1106 54-55 0.5064111145824555 1106 56-57 0.5629159259193983 1106 58-59 0.6579813755496353 1106 60-61 0.7046942848361049 1106 62-63 0.5403478875026728 1106 64-65 0.6339831630245314 1106 66-67 0.5610449575908767 1106 68-69 0.6443814247865411 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-0.5968082390545923 2216 108-109 -0.6727766219488167 2216 110-111 -0.6593206183774214 2216 112-113 -0.7572149074345198 2216 114-115 -0.8083401567291943 2216 116-117 -0.9583730142512543 2216 118-119 -0.978585613111818 2216 120-121 -1.0026562155709406 2216 122-123 -0.7285882201718934 2216 124-125 -1.0299201074559399 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 144791.0 3 23210.0 4 14688.0 5 11993.0 6 11484.0 7 11697.0 8 12281.0 9 13016.0 10 13837.0 11 14934.0 12 15535.0 13 17532.0 14 19952.0 15 17271.0 16 12887.0 17 11705.0 18 10515.0 19 9675.0 20 9484.0 21 9984.0 22 11673.0 23 15528.0 24 21295.0 25 26852.0 26 32339.0 27 34580.0 28 39059.0 29 45549.0 30 50118.0 31 57482.0 32 68851.0 33 86550.0 34 109545.0 35 149303.0 36 214251.0 37 240590.0 38 66049.0 39 158.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.382573945044335 18.52149289814933 13.175564812814953 22.920368343991377 2 15.468227225215852 20.523437015258757 39.77312320123712 24.235212558288268 3 18.631149443323128 27.223023351134508 28.09313486549486 26.0526923400475 4 12.309073715548848 16.872570632508108 36.335253459657956 34.483102192285095 5 13.875121046630474 39.1152900912705 32.08864879442628 14.920940067672747 6 31.197501049979 36.73029380321485 17.69471144668016 14.377493700126 7 29.213853851082796 33.662863054448174 19.796721726170944 17.326561368298087 8 27.60977972764092 34.23196994707808 19.096932843269144 19.061317482011855 9 28.28400940700323 16.71210680361151 18.64749868452295 36.35638510486231 10-11 25.470978849725253 27.56354538095014 25.821554512084465 21.14392125724015 12-13 26.30907626008126 25.0255415326948 26.50389290245178 22.161489304772157 14-15 23.285027037106097 25.922565728137236 24.923214618683573 25.869192616073093 16-17 22.97946502179173 29.665275349761195 24.836364363310647 22.518895265136425 18-19 22.79379433904067 27.37119715633533 27.698465588042097 22.1365429165819 20-21 24.007092653453658 26.4477279256226 27.418522934633096 22.12665648629065 22-23 23.454482596308758 26.473912435222186 27.272708290516018 22.79889667795304 24-25 23.09755313921967 26.68662634755441 27.569567771280674 22.646252741945254 26-27 23.066772636984236 27.055009693061987 27.291623034462432 22.586594635491345 28-29 22.58899048908788 26.74778111031733 27.775413016988992 22.887815383605794 30-31 22.6835880883071 26.637070286481148 27.77689782089865 22.9024438043131 32-33 22.810171781941193 26.749298598091116 27.403373590297758 23.037156029669934 34-35 23.231269200043194 26.583807797014863 27.342860194152745 22.842062808789198 36-37 23.44103272567158 26.362540455512136 27.14138644012077 23.055040378695512 38-39 22.983541169150296 26.31074372868373 27.465498737354903 23.24021636481107 40-41 23.061096501670487 26.436623172502188 27.493962790381282 23.008317535446043 42-43 23.256962113300037 26.44142809796255 26.98137539791282 23.320234390824595 44-45 23.12099600174465 26.74976625630004 26.74550009815147 23.383737643803837 46-47 23.1486657483103 26.965910046814695 26.284630019514577 23.600794185360428 48-49 23.553262599374527 26.777463709612455 26.280434428474507 23.38883926253851 50-51 23.335833081270444 27.010313399185883 25.975602698773216 23.67825082077046 52-53 23.68761569772402 26.984005302333852 25.839242878270035 23.489136121672097 54-55 23.273922891129185 26.822051495016613 25.980567648069652 23.92345796578455 56-57 23.334622976307063 27.22923538092245 25.94586661472299 23.490275028047495 58-59 23.67034881969362 26.576707312235104 25.987888954942868 23.76505491312841 60-61 23.538178201731437 26.736881920129058 26.349035514703385 23.375904363436124 62-63 23.251223355232046 26.87490881337899 26.15427717092781 23.719590660461154 64-65 23.10633430570613 26.71558960816101 26.18491153475989 23.993164551372967 66-67 23.227707243387844 27.247217907764025 25.74695973628626 23.77811511256187 68-69 23.000866091718606 26.865043338895454 26.33942143402472 23.794669135361215 70-71 23.005167344340574 27.041590216439033 26.287742002070758 23.665500437149635 72-73 23.32551736070714 26.888586591728792 26.039181291783386 23.746714755780687 74-75 23.025390710058147 27.281575523357894 25.841987110460714 23.85104665612325 76-77 23.248556889330242 27.118429189212083 25.95102417529182 23.68198974616585 78-79 23.395351695238823 27.05825713031194 25.984295654995993 23.562095519453248 80-81 22.966622499014132 27.764559963106784 25.937737331530037 23.331080206349046 82-83 23.293369394640738 27.473848144589656 25.626178433075143 23.606604027694463 84-85 23.26825525893322 27.043185266098053 25.568786864434333 24.11977261053439 86-87 23.247405289680135 26.793119114303877 25.961362061461664 23.998113534554324 88-89 23.26909738019341 26.878830775381008 25.890395633605962 23.961676210819626 90-91 23.257023296971873 27.250061086416324 26.011088340654254 23.48182727595755 92-93 23.192270714703547 26.799958596097472 26.027155352036086 23.9806153371629 94-95 23.147456407404444 26.85036560376834 25.98710630614288 24.015071682684333 96-97 23.29176619896555 26.92403504014258 25.839614048873976 23.944584712017893 98-99 23.18149630091867 26.83860837464138 25.620336625051383 24.359558699388568 100-101 22.85541606557103 26.909187127783085 25.986274137793195 24.249122668852685 102-103 23.435146336181568 26.501348551492832 26.037722454321955 24.025782658003642 104-105 23.00617102030638 26.590660425647677 26.190683572939623 24.21248498110632 106-107 23.015554133724685 26.968263840106665 26.080653604714115 23.93552842145453 108-109 23.230372923257548 26.97815889462327 25.5380037381215 24.25346444399768 110-111 22.72883196220089 27.162322592961587 26.030109233219783 24.078736211617738 112-113 23.057277494969405 26.912864065651583 25.961331382144476 24.068527057234544 114-115 22.46303191124437 27.21464012079394 25.854067697821854 24.46826027013983 116-117 22.568565536142433 27.337504168548353 26.005656697746087 24.088273597563123 118-119 22.633353278731068 27.46027873047046 25.54775769384272 24.358610296955753 120-121 22.564194191530817 27.49313643256761 25.802330451092388 24.14033892480919 122-123 22.633144478455687 27.417534331239562 25.68750473529196 24.261816455012788 124-125 22.784954394825554 27.504734160514822 25.45535806722732 24.254953377432308 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1692.0 1 1805.5 2 2098.0 3 1854.0 4 1182.5 5 820.0 6 690.0 7 822.0 8 1150.0 9 1294.0 10 1227.5 11 1126.0 12 1153.5 13 1310.5 14 1433.5 15 1609.0 16 1818.5 17 1953.5 18 2092.5 19 2279.5 20 2570.5 21 3003.0 22 3565.5 23 4188.0 24 4978.0 25 6119.0 26 7682.0 27 9528.0 28 11379.0 29 13362.5 30 15828.5 31 18579.0 32 21345.0 33 24438.5 34 28493.5 35 31068.5 36 32955.5 37 36426.0 38 38905.0 39 40010.5 40 40840.5 41 44319.0 42 48460.0 43 51153.0 44 52920.5 45 54403.0 46 55212.5 47 55818.5 48 59908.0 49 62713.5 50 62782.5 51 61979.0 52 61020.0 53 61721.5 54 61602.0 55 58115.5 56 53163.5 57 49014.5 58 45882.5 59 42547.5 60 38400.5 61 33177.5 62 28065.5 63 23131.0 64 18593.5 65 15341.5 66 12149.5 67 9939.0 68 7409.5 69 4828.5 70 3773.5 71 3126.0 72 2614.0 73 2309.0 74 1915.5 75 1556.0 76 1077.0 77 642.5 78 430.5 79 313.0 80 218.0 81 150.0 82 115.0 83 83.5 84 70.5 85 61.0 86 39.0 87 30.5 88 21.5 89 17.5 90 17.5 91 12.0 92 6.0 93 5.5 94 8.0 95 6.0 96 3.0 97 4.0 98 3.5 99 2.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011991101528835616 2 0.0054288071598211 3 0.008113382128963403 4 0.008232696572036393 5 0.014079104282612963 6 0.0011334872091934166 7 0.006979894919769986 8 0.0 9 0.002684574969142302 10-11 0.0 12-13 0.0030723469091295235 14-15 0.018225281179399408 16-17 0.029381181606724087 18-19 0.005727093267503578 20-21 0.008501154068950623 22-23 0.0019388596999361071 24-25 0.011782301253457882 26-27 0.01801648090402167 28-29 6.264008261332039E-4 30-31 0.0014616019276441421 32-33 0.008083553518195154 34-35 0.008352011015109384 36-37 0.010350527936581987 38-39 0.0 40-41 0.023027687513087303 42-43 0.009157383505852075 44-45 0.015630192042561847 46-47 0.0035794332921897363 48-49 0.023027687513087303 50-51 0.004414634393700674 52-53 0.0 54-55 0.01640573592253629 56-57 0.004086519675249949 58-59 2.6845749691423024E-4 60-61 0.0038180621783357187 62-63 0.025145518877632896 64-65 0.016346078700999796 66-67 0.0018792024783996115 68-69 0.019299111167056328 70-71 0.0025950891368375587 72-73 0.011215557648861172 74-75 0.0 76-77 0.005339321327516356 78-79 0.0011334872091934166 80-81 0.0032214899629707623 82-83 0.0014019447061076465 84-85 0.0 86-87 0.0055182929921258434 88-89 0.015540706210257103 90-91 0.020193969490103762 92-93 0.0043251485613959315 94-95 0.022968030291550805 96-97 0.01667419341945052 98-99 9.545155445839297E-4 100-101 0.008173039350499898 102-103 0.0 104-105 0.0012528016522664078 106-107 2.9828610768247802E-5 108-109 0.0 110-111 7.755438799744429E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 5.9657221536495605E-5 122-123 0.0 124-125 0.02335580223153803 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1676243.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.91916426637804 #Duplication Level Percentage of deduplicated Percentage of total 1 80.12733179034657 43.203987650302714 2 9.910278561507898 10.687078753670182 3 3.846222139662575 6.221552500603392 4 1.8609844919160135 4.013709140672064 5 1.1005078331485245 2.9669231315985525 6 0.679652069840936 2.198776295864236 7 0.4456547951847381 1.6820533875365467 8 0.34230352315104606 1.476537591499295 9 0.2321522813764919 1.1265711294918135 >10 1.2647062625823045 12.484653077261317 >50 0.11042633956502307 4.155710327349576 >100 0.07568295415022509 7.454726911872064 >500 0.002845109421952034 1.0271446932049222 >1k 0.0012518481456588947 1.3005754090733213 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3887 0.23188762011235842 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2735 0.16316250090231546 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2056 0.12265524747903495 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 5.9657221536495605E-5 0.0 0.0 0.0 0.0 3 5.9657221536495605E-5 0.0 0.0 0.0 0.0 4 5.9657221536495605E-5 0.0 0.0 0.0 0.0 5 5.9657221536495605E-5 0.0 0.0 0.0 0.0 6 5.9657221536495605E-5 0.0 0.0 0.0 0.0 7 5.9657221536495605E-5 0.0 0.0 0.0 0.0 8 5.9657221536495605E-5 0.0 0.0 0.0 0.0 9 5.9657221536495605E-5 0.0 0.0 0.0 0.0 10-11 5.9657221536495605E-5 0.0 0.0 0.0 0.0 12-13 5.9657221536495605E-5 0.0 0.0 0.0 0.0 14-15 1.4914305384123902E-4 0.0 0.0 0.0 0.0 16-17 1.7897166460948682E-4 0.0 0.0 0.0 0.0 18-19 2.0880027537773462E-4 0.0 0.0 0.0 0.0 20-21 2.9828610768247804E-4 0.0 0.0 1.1931444307299121E-4 0.0 22-23 2.9828610768247804E-4 0.0 0.0 4.47429161523717E-4 0.0 24-25 4.1760055075546924E-4 0.0 0.0 9.246869338156818E-4 0.0 26-27 4.1760055075546924E-4 0.0 0.0 0.0012528016522664078 0.0 28-29 5.369149938284604E-4 0.0 0.0 0.0020581741430090983 0.0 30-31 5.369149938284604E-4 0.0 0.0 0.004414634393700675 0.0 32-33 5.369149938284604E-4 0.0 0.0 0.008560811290487119 0.0 34-35 5.369149938284604E-4 0.0 0.0 0.014616019276441423 0.0 36-37 5.369149938284604E-4 0.0 0.0 0.023504945285379268 0.0 38-39 6.562294369014517E-4 0.0 0.0 0.04322165700319107 0.0 40-41 6.562294369014517E-4 0.0 0.0 0.07078329335305203 0.0 42-43 7.755438799744429E-4 0.0 0.0 0.09685349916450062 0.0 44-45 8.948583230474341E-4 0.0 0.0 0.1253696510589455 2.9828610768247802E-5 46-47 0.001044001376888673 5.9657221536495605E-5 0.0 0.16176055619620783 5.9657221536495605E-5 48-49 0.0010738299876569208 5.9657221536495605E-5 0.0 0.2032223251640723 5.9657221536495605E-5 50-51 0.0010738299876569208 5.9657221536495605E-5 0.0 0.25801748314534345 5.9657221536495605E-5 52-53 0.0010738299876569208 5.9657221536495605E-5 0.0 0.3089647503375107 5.9657221536495605E-5 54-55 0.0011334872091934166 5.9657221536495605E-5 0.0 0.36340196498956295 5.9657221536495605E-5 56-57 0.0011334872091934166 5.9657221536495605E-5 0.0 0.4207922121076717 5.9657221536495605E-5 58-59 0.0011334872091934166 5.9657221536495605E-5 0.0 0.4934248793283551 5.9657221536495605E-5 60-61 0.0011334872091934166 5.9657221536495605E-5 0.0 0.5772432755871315 5.9657221536495605E-5 62-63 0.0011334872091934166 5.9657221536495605E-5 0.0 0.6644024762519516 5.9657221536495605E-5 64-65 0.0011334872091934166 5.9657221536495605E-5 0.0 0.746431155864633 5.9657221536495605E-5 66-67 0.0011931444307299122 5.9657221536495605E-5 0.0 0.8396753931261756 5.9657221536495605E-5 68-69 0.0011931444307299122 5.9657221536495605E-5 0.0 0.9527258279378348 5.9657221536495605E-5 70-71 0.0012528016522664078 5.9657221536495605E-5 0.0 1.0875809772210832 5.9657221536495605E-5 72-73 0.0012528016522664078 5.9657221536495605E-5 0.0 1.2240468714858168 5.9657221536495605E-5 74-75 0.0012528016522664078 5.9657221536495605E-5 0.0 1.3562472744106913 5.9657221536495605E-5 76-77 0.0012528016522664078 5.9657221536495605E-5 0.0 1.5040182121565908 5.9657221536495605E-5 78-79 0.0012528016522664078 5.9657221536495605E-5 0.0 1.6726691774402638 5.9657221536495605E-5 80-81 0.0012528016522664078 5.9657221536495605E-5 0.0 1.87275949847367 5.9657221536495605E-5 82-83 0.0012528016522664078 5.9657221536495605E-5 0.0 2.091045272075707 5.9657221536495605E-5 84-85 0.0012826302630346556 5.9657221536495605E-5 0.0 2.30804841541471 5.9657221536495605E-5 86-87 0.0013124588738029034 5.9657221536495605E-5 0.0 2.5268114467890395 5.9657221536495605E-5 88-89 0.0013124588738029034 5.9657221536495605E-5 0.0 2.7690197662272116 5.9657221536495605E-5 90-91 0.0014317733168758946 5.9657221536495605E-5 0.0 3.054121627950124 5.9657221536495605E-5 92-93 0.0014914305384123902 5.9657221536495605E-5 0.0 3.3742422787149593 5.9657221536495605E-5 94-95 0.0014914305384123902 5.9657221536495605E-5 0.0 3.683057885998629 5.9657221536495605E-5 96-97 0.0014914305384123902 5.9657221536495605E-5 0.0 3.995781041292939 5.9657221536495605E-5 98-99 0.001521259149180638 5.9657221536495605E-5 0.0 4.332695199920298 5.9657221536495605E-5 100-101 0.0017300594245583726 5.9657221536495605E-5 0.0 4.729356065916457 5.9657221536495605E-5 102-103 0.0019090310891678593 5.9657221536495605E-5 0.0 5.1654801839590085 5.9657221536495605E-5 104-105 0.0023564602506915763 5.9657221536495605E-5 0.0 5.592655718771085 5.9657221536495605E-5 106-107 0.0025354319153010636 5.9657221536495605E-5 0.0 6.013149644771074 5.9657221536495605E-5 108-109 0.0026547463583740548 1.1931444307299121E-4 0.0 6.45174953750739 5.9657221536495605E-5 110-111 0.0026845749691423023 1.1931444307299121E-4 0.0 6.951259453432467 5.9657221536495605E-5 112-113 0.0026845749691423023 1.1931444307299121E-4 0.0 7.505445212895744 5.9657221536495605E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7720 0.0 48.39921 1 GGTATCA 4725 0.0 39.79067 1 GTACATG 12335 0.0 38.587513 1 TATCAAC 9860 0.0 37.71369 2 ATCAACG 9680 0.0 37.55448 3 TCAACGC 9675 0.0 37.327908 4 CAACGCA 9935 0.0 36.410923 5 AACGCAG 9935 0.0 36.347782 6 TACATGG 12920 0.0 36.195618 2 ACATGGG 13260 0.0 34.50474 3 CATGGGG 5020 0.0 32.000446 4 ACGCAGA 11445 0.0 31.3443 7 CGCAGAG 11460 0.0 30.685816 8 CATGGGA 8205 0.0 28.280167 4 GCAGAGT 11945 0.0 27.99529 9 ATGGGGA 2995 0.0 26.8184 5 ATGGGAG 3195 0.0 26.629383 5 GAGTACT 3950 0.0 26.44032 12-13 AGTACTT 4050 0.0 24.979319 12-13 CATGGGT 1650 0.0 23.43826 4 >>END_MODULE