FastQCFastQC Report
Thu 26 May 2016
SRR1364537_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364537_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1676243
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79280.47296245234133716No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66700.3979136676484257No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45830.2734090463017594No Hit
TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG22420.13375149068482314No Hit
TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC20180.12038827306064812No Hit
CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG18750.11185729038092926No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18070.10780059931644755No Hit
CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG17020.10153659105511552No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64100.053.101121
GTATCAA106600.049.4893341
TATCAAC141900.037.0685352
ATCAACG142500.036.9124573
TCAACGC142550.036.7741474
CAACGCA144400.036.1371545
AACGCAG146300.035.708516
TAACGCA1451.08704626E-732.8661734
ACGCAGA168050.030.874477
CGCAGAG169900.030.5733058
GTACATG120900.029.8300951
TACATGG123050.028.7078192
GCAGAGT175350.028.6033849
GAGTACT95700.027.88342712-13
ACATGGG126100.027.5410583
AGTACTT96900.027.50742112-13
TATAACG1107.6996995E-427.0772442
GTACTTT104450.025.91782614-15
ACTTGAC11050.025.3079598
CATGGGG47600.025.2796784
Produced by FastQC (version 0.11.5)