##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364537_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1676243 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.722274753720075 31.0 30.0 34.0 26.0 34.0 2 29.62082585878062 31.0 30.0 34.0 25.0 34.0 3 29.881001143628936 31.0 30.0 34.0 26.0 34.0 4 33.40979201702856 35.0 33.0 37.0 28.0 37.0 5 32.828304130129105 35.0 33.0 37.0 26.0 37.0 6 32.838597387133014 35.0 33.0 37.0 26.0 37.0 7 32.605383586985894 35.0 33.0 37.0 25.0 37.0 8 32.51683675934814 35.0 33.0 37.0 24.0 37.0 9 33.69582154854636 38.0 33.0 39.0 22.0 39.0 10-11 33.621770829169755 37.5 33.0 39.0 22.0 39.0 12-13 33.494661275244695 37.0 33.0 39.0 20.5 39.0 14-15 34.38674315120183 38.0 33.0 41.0 18.5 41.0 16-17 34.40009801681498 38.0 33.0 41.0 19.0 41.0 18-19 34.432608219691296 38.5 33.0 41.0 19.0 41.0 20-21 34.46534899772885 39.0 33.0 41.0 20.0 41.0 22-23 34.42264784998356 39.0 33.0 41.0 21.0 41.0 24-25 34.36795709213999 39.0 33.0 41.0 21.0 41.0 26-27 34.22382971919943 38.5 33.0 41.0 19.0 41.0 28-29 34.04643151380796 38.0 33.0 40.5 18.5 41.0 30-31 33.8267390229221 38.0 33.0 40.0 18.0 41.0 32-33 33.5556041099053 38.0 32.5 40.0 16.5 41.0 34-35 33.324969291445214 38.0 32.0 40.0 15.5 41.0 36-37 33.06911229457781 38.0 32.0 40.0 13.0 41.0 38-39 32.86608713653092 38.0 31.5 40.0 9.0 41.0 40-41 32.839962046075655 38.0 32.0 40.0 9.0 41.0 42-43 32.64419210102592 38.0 31.0 40.0 7.5 41.0 44-45 32.39020386662315 38.0 31.0 40.0 2.0 41.0 46-47 32.00735573541545 37.5 30.5 40.0 2.0 41.0 48-49 31.60984833344569 37.0 30.0 40.0 2.0 41.0 50-51 31.38623815282152 37.0 30.0 40.0 2.0 41.0 52-53 31.168042759910108 37.0 29.5 40.0 2.0 41.0 54-55 30.948588003052066 36.0 29.0 40.0 2.0 41.0 56-57 30.709658444509536 36.0 29.0 40.0 2.0 41.0 58-59 30.487348493028755 35.5 28.5 40.0 2.0 41.0 60-61 30.231419907495514 35.0 28.0 39.0 2.0 41.0 62-63 30.050779332113542 35.0 28.0 39.0 2.0 41.0 64-65 29.918750145414478 35.0 28.0 39.0 2.0 41.0 66-67 29.621334138308107 35.0 28.0 39.0 2.0 40.5 68-69 29.34294759172745 35.0 28.0 38.0 2.0 40.0 70-71 29.041742754481298 35.0 28.0 37.0 2.0 40.0 72-73 28.76492191167987 34.5 28.0 37.0 2.0 39.0 74-75 28.43317317357925 34.0 27.0 36.0 2.0 39.0 76-77 28.145512911910743 34.0 27.0 36.0 2.0 39.0 78-79 27.876301049430182 34.0 26.0 35.0 2.0 37.5 80-81 27.618135019803212 34.0 26.0 35.0 2.0 37.0 82-83 27.365668342835733 34.0 26.0 35.0 2.0 36.5 84-85 27.16613432539316 34.0 26.0 35.0 2.0 36.0 86-87 26.96443773366988 34.0 25.5 35.0 2.0 36.0 88-89 26.795872376499112 34.0 25.0 35.0 2.0 35.5 90-91 26.668363417475867 34.0 25.0 35.0 2.0 35.0 92-93 26.56878865415098 34.0 25.0 35.0 2.0 35.0 94-95 26.466461306624396 33.5 25.0 35.0 2.0 35.0 96-97 26.358654443299688 33.0 24.5 35.0 2.0 35.0 98-99 26.27183081450601 33.0 24.0 35.0 2.0 35.0 100-101 26.166809048568734 33.0 24.0 35.0 2.0 35.0 102-103 24.916813373717297 31.5 20.0 34.0 2.0 35.0 104-105 25.83182330962754 33.0 23.0 35.0 2.0 35.0 106-107 25.989917332988117 33.0 23.0 35.0 2.0 35.0 108-109 25.983998143467268 33.0 23.0 35.0 2.0 35.0 110-111 25.906117728754126 33.0 23.0 35.0 2.0 35.0 112-113 25.82111394350342 33.0 23.0 35.0 2.0 35.0 114-115 25.706949410079567 33.0 21.0 35.0 2.0 35.0 116-117 25.578030452625306 33.0 19.5 35.0 2.0 35.0 118-119 25.430175994769257 33.0 19.0 35.0 2.0 35.0 120-121 25.233728940255084 33.0 17.0 35.0 2.0 35.0 122-123 24.921213392091722 33.0 2.0 35.0 2.0 35.0 124-125 23.125154586775306 31.0 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9731760590255405 1101 2 1.1049023920753562 1101 3 0.9298192775790319 1101 4 0.8303949386362675 1101 5 0.9642722701226134 1101 6 1.037656192803361 1101 7 1.0864866356294058 1101 8 1.1522647754557696 1101 9 1.2678837905585354 1101 10-11 1.24455109498885 1101 12-13 1.2289937147685066 1101 14-15 1.4305681849703333 1101 16-17 1.3775748098441625 1101 18-19 1.4307014465446741 1101 20-21 1.5213685631776883 1101 22-23 1.4486500095292172 1101 24-25 1.5060545885465757 1101 26-27 1.5572246111517742 1101 28-29 1.7508531525064086 1101 30-31 1.7598834647384223 1101 32-33 1.6351386409437865 1101 34-35 1.7717451346264212 1101 36-37 1.765554851460088 1101 38-39 1.7539973291775937 1101 40-41 1.6016642474003504 1101 42-43 1.7216512991362407 1101 44-45 1.653456798125596 1101 46-47 1.5633956376094318 1101 48-49 1.5422515299721837 1101 50-51 1.5052272764266128 1101 52-53 1.52763130856437 1101 54-55 1.5710096399393834 1101 56-57 1.4977923763318763 1101 58-59 1.542713333295712 1101 60-61 1.4859085344643397 1101 62-63 1.6136754346985747 1101 64-65 1.4565651315861174 1101 66-67 1.5609456844520864 1101 68-69 1.536779265752859 1101 70-71 1.5228120535771374 1101 72-73 1.542328848813046 1101 74-75 1.5981048470679653 1101 76-77 1.702988328540929 1101 78-79 1.5452761572849596 1101 80-81 1.3559123252628922 1101 82-83 1.5672372278867464 1101 84-85 1.4772430130872785 1101 86-87 1.5567356089748507 1101 88-89 1.5688311964117752 1101 90-91 1.398208648933327 1101 92-93 1.2882380432233305 1101 94-95 1.278742269790481 1101 96-97 1.3758210378497324 1101 98-99 1.352857999055331 1101 100-101 1.3167777414524302 1101 102-103 1.189131954560402 1101 104-105 1.3193068655447817 1101 106-107 1.222681450211919 1101 108-109 1.2501128016611034 1101 110-111 1.2581064813768315 1101 112-113 1.2432113777304572 1101 114-115 1.3698793581527262 1101 116-117 1.3215579240960338 1101 118-119 1.3145280981481875 1101 120-121 1.340425324743432 1101 122-123 1.3056951942130794 1101 124-125 1.5183423070401503 1102 1 0.4566534952043533 1102 2 -0.27882032868076223 1102 3 0.22605623162541733 1102 4 0.4514717128564598 1102 5 0.7126309431374764 1102 6 0.564827470171096 1102 7 0.5579197583395441 1102 8 0.7153580750421966 1102 9 1.1711199835295005 1102 10-11 1.007486943410946 1102 12-13 1.1769576905320847 1102 14-15 1.2629564425796147 1102 16-17 1.2115403815061967 1102 18-19 1.3081843813458818 1102 20-21 1.2744530572985298 1102 22-23 1.403538222889928 1102 24-25 1.406799218245034 1102 26-27 1.3501813448992763 1102 28-29 1.0907160261855893 1102 30-31 1.4178326467298419 1102 32-33 1.575230132238616 1102 34-35 1.6220172633366658 1102 36-37 1.2572765338497405 1102 38-39 0.6214461630802575 1102 40-41 0.2875841309381144 1102 42-43 0.6577601978188099 1102 44-45 0.16047882506413202 1102 46-47 0.26813768319136244 1102 48-49 -0.13242206307076287 1102 50-51 -0.0795627936041221 1102 52-53 -0.3008653609932104 1102 54-55 -0.17346872840931837 1102 56-57 -0.14425431181009785 1102 58-59 -0.08516894485670434 1102 60-61 -0.11191627954417172 1102 62-63 -0.1808828760679866 1102 64-65 -0.1651890145049677 1102 66-67 -0.3096265085660761 1102 68-69 -0.07772128587932059 1102 70-71 -0.5448249104989245 1102 72-73 -0.48460872235340346 1102 74-75 -0.14209261955386054 1102 76-77 -0.5892563719552477 1102 78-79 -0.47537841182245444 1102 80-81 -0.5795933179048376 1102 82-83 -0.8341672954971493 1102 84-85 -0.5967608040894312 1102 86-87 -0.23886519811907903 1102 88-89 -0.34255425583812027 1102 90-91 -0.38168067064165 1102 92-93 -0.29137634523192446 1102 94-95 -0.05317348683058043 1102 96-97 -0.23965917713932328 1102 98-99 -0.3277005064049412 1102 100-101 -0.2898315938160394 1102 102-103 -0.35633514810218614 1102 104-105 -0.28865544352263584 1102 106-107 -0.1515765012562511 1102 108-109 -0.10303039556362492 1102 110-111 -0.032087740349805216 1102 112-113 0.10872072302860403 1102 114-115 0.11816531601628455 1102 116-117 -0.08894056555275043 1102 118-119 0.2302672410238742 1102 120-121 0.37382392471865344 1102 122-123 0.4347830984237788 1102 124-125 0.6473975410560939 1103 1 0.4969001204182675 1103 2 0.17655237789325895 1103 3 0.4252500531822214 1103 4 0.4608814971588089 1103 5 0.741229512222283 1103 6 0.6934093972822026 1103 7 0.7796468122978268 1103 8 0.7618760734103986 1103 9 1.0288574703852902 1103 10-11 1.0794959536406026 1103 12-13 1.1222374082370905 1103 14-15 1.2275230296200803 1103 16-17 1.1950323535948115 1103 18-19 1.2201062936706677 1103 20-21 1.2210117033193342 1103 22-23 1.3010045192113182 1103 24-25 1.2173084773679292 1103 26-27 1.3419158803820253 1103 28-29 1.2821474715630927 1103 30-31 1.144753248315098 1103 32-33 1.3406898630804633 1103 34-35 1.153947614467583 1103 36-37 1.3149648311549171 1103 38-39 1.3140393802241377 1103 40-41 1.239013647024862 1103 42-43 1.1274832930491883 1103 44-45 1.1834308313792405 1103 46-47 1.3135506839601305 1103 48-49 1.2759398659843626 1103 50-51 1.2040768944104165 1103 52-53 1.0830456059201943 1103 54-55 1.241383349481012 1103 56-57 1.2190232586384724 1103 58-59 1.2713611152605857 1103 60-61 1.2641103682321422 1103 62-63 1.2499012407144896 1103 64-65 1.105234884547471 1103 66-67 0.9531679291855752 1103 68-69 1.0188327210072146 1103 70-71 1.0564965063957743 1103 72-73 1.0295449817375761 1103 74-75 1.1078285888007073 1103 76-77 1.1346655079692347 1103 78-79 0.9470524650770109 1103 80-81 0.9030503553469487 1103 82-83 0.9240298338360198 1103 84-85 1.0724544163946312 1103 86-87 1.0548716760764343 1103 88-89 1.1742115017545984 1103 90-91 1.1145976391229198 1103 92-93 1.0798957053832439 1103 94-95 1.172532987044466 1103 96-97 1.0978001383895517 1103 98-99 1.112490007375495 1103 100-101 0.9911452311310178 1103 102-103 0.7566812317236185 1103 104-105 0.8457455043334932 1103 106-107 1.0083798172446166 1103 108-109 0.9955095289731233 1103 110-111 0.8969672311821064 1103 112-113 0.8640352563316966 1103 114-115 0.9543191202536576 1103 116-117 1.0886099273736747 1103 118-119 0.9251380521801806 1103 120-121 0.87707507419303 1103 122-123 0.9159432796298326 1103 124-125 1.171096447987697 1104 1 0.6934006131886434 1104 2 0.8602474018896409 1104 3 0.5265600127275896 1104 4 0.3441659722397077 1104 5 0.5962425085174345 1104 6 0.8223433939710034 1104 7 0.9394082723499295 1104 8 0.7033170847483063 1104 9 0.8647025366789549 1104 10-11 1.0880604563054703 1104 12-13 1.06089852460628 1104 14-15 1.1814328065751738 1104 16-17 1.24428907998022 1104 18-19 1.1464625987583474 1104 20-21 0.8678402443560742 1104 22-23 1.0173854348987277 1104 24-25 1.2564394007330577 1104 26-27 1.1663663646370992 1104 28-29 1.3056816541924476 1104 30-31 1.2066697062872294 1104 32-33 1.3161092064813928 1104 34-35 1.4367395983639 1104 36-37 1.5673558144342294 1104 38-39 1.5781626956613906 1104 40-41 1.5436951749759729 1104 42-43 1.5209022260407963 1104 44-45 1.546695637523655 1104 46-47 1.5503776615327922 1104 48-49 1.4894666221593411 1104 50-51 1.4584066429439737 1104 52-53 1.5066119651986476 1104 54-55 1.0599480196598847 1104 56-57 1.2667052137503028 1104 58-59 1.3640055053560403 1104 60-61 1.4170158289096229 1104 62-63 1.492116725151039 1104 64-65 1.5321395188225146 1104 66-67 1.4838905566986362 1104 68-69 1.1389199539270685 1104 70-71 0.7346623815587492 1104 72-73 0.8209829917828309 1104 74-75 1.2169876963977444 1104 76-77 1.2894882362698858 1104 78-79 1.3701572534649387 1104 80-81 1.3193713149788664 1104 82-83 1.2090320254776294 1104 84-85 1.3692359837115298 1104 86-87 1.2372353824913844 1104 88-89 1.1999064056992736 1104 90-91 1.2047582152594316 1104 92-93 1.3246901377759812 1104 94-95 1.3941804733571743 1104 96-97 1.3793634070345604 1104 98-99 1.3371840849177268 1104 100-101 1.27399019133248 1104 102-103 1.179090139001822 1104 104-105 1.3265977458105205 1104 106-107 1.3593768047843682 1104 108-109 1.2564058964318505 1104 110-111 1.309977067046301 1104 112-113 1.3276639168617663 1104 114-115 1.1875228396230249 1104 116-117 1.1387264194069893 1104 118-119 1.169579727990321 1104 120-121 1.0858082076263145 1104 122-123 1.136605783573394 1104 124-125 1.5744418423296338 1105 1 0.24638101713129856 1105 2 -0.019665804507340567 1105 3 0.2252408437464979 1105 4 0.332594486524485 1105 5 -0.02691836729083974 1105 6 0.4474586889278669 1105 7 0.6238666329483564 1105 8 0.5915964669335878 1105 9 0.707797521534836 1105 10-11 0.7989946314287621 1105 12-13 0.8229950988309369 1105 14-15 0.7797338090071904 1105 16-17 0.8298831607360668 1105 18-19 0.8197531747322273 1105 20-21 0.9461721105251115 1105 22-23 0.8798767580251408 1105 24-25 0.8787280775640198 1105 26-27 0.8249257585028715 1105 28-29 0.9087404608372509 1105 30-31 0.9776628379689569 1105 32-33 1.0269110170971274 1105 34-35 1.1086587842546862 1105 36-37 1.220962090060759 1105 38-39 1.1938328629181214 1105 40-41 0.9147820914939757 1105 42-43 0.8980467903924776 1105 44-45 1.0089755895228691 1105 46-47 0.9841999548988696 1105 48-49 1.0462900018664314 1105 50-51 0.9135806173677032 1105 52-53 0.9135670344466753 1105 54-55 0.8546367428148613 1105 56-57 0.8692639766998909 1105 58-59 0.5687159908559778 1105 60-61 0.6925637064237833 1105 62-63 0.8587756474638049 1105 64-65 0.7408088611989783 1105 66-67 0.8381008919296917 1105 68-69 0.8491981325402911 1105 70-71 0.8415439149692432 1105 72-73 0.8196673042085401 1105 74-75 0.8606433298468161 1105 76-77 0.8545781098521914 1105 78-79 0.8740738361160254 1105 80-81 0.8899295550069439 1105 82-83 0.7845040225333548 1105 84-85 0.790145241909002 1105 86-87 0.9581890463878473 1105 88-89 0.753860750956779 1105 90-91 0.681067241978873 1105 92-93 0.7865560865623564 1105 94-95 0.7849663973900363 1105 96-97 0.7803107049300166 1105 98-99 0.7451679453999951 1105 100-101 0.7960665733976917 1105 102-103 0.43903435123406354 1105 104-105 0.6399720318556916 1105 106-107 0.6931641419841945 1105 108-109 0.6384765803577714 1105 110-111 0.46206864651793467 1105 112-113 0.4126220369589362 1105 114-115 0.5849912174366558 1105 116-117 0.34051309158706644 1105 118-119 0.4677504973883302 1105 120-121 0.678406452570993 1105 122-123 0.5596916315126492 1105 124-125 1.0587308928789483 1106 1 0.027052015273760333 1106 2 -0.3002927569730396 1106 3 0.17286884847147732 1106 4 0.04443389115005658 1106 5 0.37300860922776025 1106 6 0.52809774845813 1106 7 0.6617428368940566 1106 8 0.5521860663202034 1106 9 0.6631061835239151 1106 10-11 0.6607433869734933 1106 12-13 0.6299133628068603 1106 14-15 0.7387407688104162 1106 16-17 0.756985073930764 1106 18-19 0.7235788898735223 1106 20-21 0.7982585197881633 1106 22-23 0.7742846866573672 1106 24-25 0.8389255165241138 1106 26-27 0.904666535404111 1106 28-29 1.0767667293564003 1106 30-31 0.9473798595196783 1106 32-33 0.8956024999237755 1106 34-35 1.0033347246005704 1106 36-37 0.9599098864797355 1106 38-39 0.9941794532917214 1106 40-41 1.0467910155438318 1106 42-43 0.8074805975087642 1106 44-45 0.9398365557164539 1106 46-47 0.9576778616313817 1106 48-49 1.2247035747945674 1106 50-51 1.2629704931354446 1106 52-53 1.0800644562721935 1106 54-55 1.038981165056505 1106 56-57 0.5158596416379062 1106 58-59 1.0012257017917534 1106 60-61 1.1273038435267644 1106 62-63 1.0658001487326274 1106 64-65 1.1615492888128784 1106 66-67 0.9706281771637748 1106 68-69 1.095006721022191 1106 70-71 1.1157742772299102 1106 72-73 1.2057507465078992 1106 74-75 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sequence quality scores pass #Quality Count 2 63021.0 3 20624.0 4 10080.0 5 8414.0 6 9235.0 7 10981.0 8 12201.0 9 13175.0 10 14714.0 11 15855.0 12 17460.0 13 20571.0 14 25278.0 15 20832.0 16 13070.0 17 11170.0 18 10288.0 19 9491.0 20 9428.0 21 9476.0 22 10696.0 23 13101.0 24 17466.0 25 22992.0 26 28407.0 27 30496.0 28 34936.0 29 41089.0 30 47308.0 31 55159.0 32 65923.0 33 82361.0 34 106025.0 35 148881.0 36 235231.0 37 314083.0 38 96432.0 39 293.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.307998777062274 19.255357518200753 14.638051038541647 22.798592666195326 2 16.143681010069557 21.2202779910526 37.785437774162546 24.8506032247153 3 18.936753203443654 26.38054267788143 29.300107442655985 25.38259667601893 4 13.024745655223317 17.445092759631123 35.451216023915 34.07894556123055 5 13.595582502059667 38.905158738917926 32.28565309444991 15.2136056645725 6 31.692421683490995 36.765433174068434 16.79422374918195 14.747921393258615 7 28.33943439135384 33.74435930538619 20.06637585221917 17.8498304510408 8 27.69388824123913 33.76758349213016 18.863153925732007 19.675374340898706 9 27.858595982555677 15.533203276478202 18.916590601304147 37.691610139661975 10-11 25.75981382063233 26.888743987261915 25.74248329438688 21.608958897718875 12-13 26.753758255813747 24.321115733220065 27.00682418957156 21.918301821394632 14-15 23.633096046663894 24.939321130445027 25.82964577022716 25.597937052663923 16-17 22.715234138817358 28.56058371168542 25.98827672792386 22.735905421573364 18-19 22.4453940058316 25.59165380284421 29.846961527849242 22.115990663474946 20-21 23.487277509665322 24.75274045928812 29.85772490498737 21.902257126059187 22-23 22.884122409459724 24.805741172371786 29.875710144650863 22.434426273517623 24-25 22.750669204882584 25.147964823715895 29.896172571637887 22.205193399763637 26-27 22.625239896602103 25.497585970530523 29.726298633312716 22.150875499554658 28-29 22.129309413969217 25.17287171370738 30.338739669606372 22.359079202717027 30-31 22.268937140975385 24.962222064462015 30.351536143625957 22.417304650936646 32-33 22.356692913855568 25.138926754653113 30.035859955865586 22.46852037562573 34-35 22.834338458087522 25.168188621816768 29.598423378949235 22.399049541146482 36-37 23.099842922535693 24.82322073828198 29.42962327061172 22.647313068570607 38-39 22.656201994579543 24.91139411171292 29.62168969534847 22.81071419835907 40-41 22.57849846352826 24.855316323468614 29.975427190449118 22.59075802255401 42-43 23.010416747452485 24.97627134013386 29.185028662312085 22.828283250101567 44-45 22.982616482216482 25.270411270919553 28.720627021261237 23.026345225602732 46-47 23.074279803107306 25.669876026327927 27.99713406707739 23.25871010348738 48-49 23.61113513971423 25.617228528321967 27.552956224127406 23.21868010783639 50-51 23.347248384407386 25.900608056411883 27.195110492664398 23.557033066516333 52-53 23.676250997021313 25.956111375260033 27.02311061105102 23.344527016667634 54-55 23.174175820570166 25.778422340913583 27.193849579088475 23.85355225942778 56-57 23.324598880391093 26.189815651733973 27.042767198788155 23.44281826908678 58-59 23.489660422293134 25.60894126041251 27.191481593505422 23.709916723788936 60-61 23.49161189636586 25.680733640647567 27.576341854969716 23.251312608016857 62-63 23.20492911827223 25.78292646113958 27.44247104984182 23.569673370746365 64-65 23.031074057226377 25.63622398377318 27.478116913623516 23.854585045376922 66-67 23.130148791076234 26.125597541645213 27.11560913304336 23.628644534235193 68-69 22.93295184528735 25.8448208284837 27.52279949864071 23.69942782758824 70-71 22.942705801008564 25.98158500885611 27.38776537769285 23.68794381244247 72-73 23.239232020655717 25.891025346563712 27.180575847296602 23.689166785483966 74-75 23.04477426733193 26.18433922287624 26.880847048969418 23.890039460822404 76-77 23.160931917389068 26.15423300798273 27.034445483142953 23.650389591485244 78-79 23.203974602727648 26.017170541502633 27.070925874112522 23.707928981657194 80-81 22.801099960835 26.71232039058811 26.95026343161173 23.536316216965155 82-83 23.049581713391195 26.464390902750974 26.676830268642433 23.809197115215394 84-85 23.00543536945419 25.99473942620491 26.60461520197251 24.39521000236839 86-87 23.090476738754464 25.735260341131927 26.91644349894377 24.25781942116984 88-89 22.966835866458144 25.91906747519051 26.877084111993423 24.237012546357928 90-91 23.014861210456957 26.179199555195755 26.990239481984414 23.815699752362875 92-93 22.942578972220907 25.79243667173466 26.981788938296088 24.28319541774835 94-95 22.82936388977132 25.83513423624565 26.985892554329098 24.34960931965393 96-97 23.06047475930166 25.819657093423025 26.7821819836079 24.337686163667417 98-99 22.967058240272333 25.766867688570265 26.58011300061956 24.685961070537836 100-101 22.606121258853694 25.863743003178307 26.884290876875326 24.645844861092677 102-103 23.143392694257336 25.35575092632751 26.956652466259367 24.54420391315579 104-105 22.80113921430246 25.376094038871454 27.114654617498772 24.70811212932731 106-107 22.82522742451672 25.76252874406662 27.014843855100967 24.397399976315697 108-109 22.959887080811075 25.695677774642462 26.45866380948347 24.885771335062994 110-111 22.46729438070063 25.886611770025908 26.902277178520272 24.74381667075319 112-113 22.69256307110604 25.654037034009985 26.84619712058455 24.80720277429943 114-115 22.15615516366064 25.829488922548823 26.678470842234685 25.335885071555854 116-117 22.180704110322907 25.80294145896508 26.985287932596886 25.03106649811513 118-119 22.179212679784495 25.99575359897103 26.56694166657221 25.258092054672264 120-121 22.092430146734216 26.049439575045874 26.746540446765 25.11158983145491 122-123 22.182730759570475 25.976615751018244 26.50514177849396 25.335511710917324 124-125 22.095023776692244 25.973927884481906 26.444864315079307 25.48618402374654 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 542.0 1 416.0 2 531.5 3 751.5 4 511.0 5 356.5 6 661.0 7 1657.0 8 2837.0 9 3206.5 10 2512.0 11 1659.5 12 1441.0 13 1542.0 14 1740.0 15 1970.5 16 2271.5 17 2601.5 18 2936.0 19 3221.5 20 3565.0 21 4072.5 22 4557.0 23 5152.0 24 6016.0 25 7010.5 26 8434.5 27 10173.0 28 12016.5 29 14026.5 30 16224.0 31 18810.0 32 21483.0 33 24204.0 34 27848.5 35 30310.5 36 32323.0 37 35882.5 38 38461.0 39 39201.5 40 39469.0 41 43066.5 42 48130.5 43 50977.0 44 51861.5 45 53292.5 46 54475.5 47 55333.0 48 60543.0 49 63571.0 50 63315.0 51 62374.0 52 60786.0 53 61702.0 54 62027.5 55 58445.0 56 53349.5 57 49310.0 58 46360.0 59 42468.5 60 37680.0 61 32627.5 62 27832.5 63 22822.0 64 18460.0 65 15311.5 66 11616.0 67 9414.5 68 6784.0 69 4008.0 70 3088.0 71 2651.5 72 2407.0 73 2179.0 74 1801.0 75 1516.5 76 976.0 77 503.0 78 295.5 79 176.5 80 125.0 81 77.0 82 49.0 83 35.5 84 28.0 85 21.0 86 13.0 87 6.0 88 3.5 89 4.0 90 2.5 91 0.5 92 0.5 93 1.0 94 0.5 95 0.5 96 1.0 97 1.0 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09467601057841853 2 1.1931444307299121E-4 3 0.0 4 0.09819578664907176 5 0.0 6 0.0 7 0.0018493738676313635 8 0.005786750489040073 9 0.0031618327414342667 10-11 5.965722153649561E-4 12-13 0.0 14-15 2.9828610768247802E-5 16-17 2.6845749691423024E-4 18-19 0.001819545256863116 20-21 0.0011036585984251686 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 2.9828610768247802E-5 52-53 0.0 54-55 0.0 56-57 0.007278181027452464 58-59 8.352011015109385E-4 60-61 0.0 62-63 0.0 64-65 3.2811471845072584E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0014019447061076465 76-77 0.0 78-79 0.0 80-81 8.948583230474341E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0011633158199616642 90-91 0.0 92-93 0.0021774885860820895 94-95 2.9828610768247802E-5 96-97 0.007785267410512677 98-99 0.003430290238348497 100-101 0.002117831364545594 102-103 0.0 104-105 0.0 106-107 0.0016405735922536292 108-109 0.0 110-111 1.1931444307299121E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 8.948583230474341E-4 122-123 0.004295319950627684 124-125 0.08283405210342415 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1676243.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.41495248440119 #Duplication Level Percentage of deduplicated Percentage of total 1 75.49492722985912 35.040934601872365 2 11.813888873877957 10.966821814740825 3 4.703419179887438 6.5492693324608995 4 2.413958127720519 4.4817500718992775 5 1.3914959279558716 3.2293108689154764 6 0.897879656724313 2.5005024962141666 7 0.5891210528846328 1.9140817974040443 8 0.4350447474690328 1.6154065025890778 9 0.31296322087741835 1.3073555723751473 >10 1.679392751443854 14.17422942062606 >50 0.154385794005371 4.989524218153666 >100 0.10664729078309296 9.415212833006926 >500 0.0051895445368396985 1.6983917372694366 >1k 0.0014271247476309171 1.2381446784142989 >5k 2.594772268419849E-4 0.8790640540582626 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7928 0.47296245234133716 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6670 0.3979136676484257 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4583 0.2734090463017594 No Hit TCCTGGAAGAGTGCACGGCCCCCGCGATCGTTCTGAAACTCGAGGAGACG 2242 0.13375149068482314 No Hit TGCATGCACTGCCTCAGTGACCAGTAAAGTCACGTGGCTTTGGGGAAGTC 2018 0.12038827306064812 No Hit CTTCCAGGATGTGCAGAAGCCATCTCAAGATGAATGGGGTAAAACCCAGG 1875 0.11185729038092926 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1807 0.10780059931644755 No Hit CTACTGGAACTGCACAAACTGGCTACTGACAAGAATGATCCCCACTTATG 1702 0.10153659105511552 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.1931444307299121E-4 0.0 0.0 0.0 0.0 3 1.1931444307299121E-4 0.0 0.0 0.0 0.0 4 1.1931444307299121E-4 0.0 0.0 0.0 0.0 5 1.1931444307299121E-4 0.0 0.0 0.0 0.0 6 1.1931444307299121E-4 0.0 0.0 0.0 0.0 7 2.9828610768247804E-4 0.0 0.0 0.0 0.0 8 2.9828610768247804E-4 0.0 0.0 0.0 0.0 9 2.9828610768247804E-4 0.0 0.0 0.0 0.0 10-11 2.9828610768247804E-4 0.0 0.0 0.0 0.0 12-13 2.9828610768247804E-4 0.0 0.0 0.0 0.0 14-15 2.9828610768247804E-4 2.9828610768247802E-5 0.0 0.0 0.0 16-17 2.9828610768247804E-4 5.9657221536495605E-5 0.0 0.0 0.0 18-19 3.5794332921897364E-4 5.9657221536495605E-5 0.0 0.0 0.0 20-21 4.1760055075546924E-4 5.9657221536495605E-5 0.0 8.948583230474341E-5 0.0 22-23 4.7725777229196484E-4 5.9657221536495605E-5 0.0 3.8777193998722144E-4 0.0 24-25 5.369149938284604E-4 5.9657221536495605E-5 0.0 8.650297122791863E-4 0.0 26-27 5.369149938284604E-4 5.9657221536495605E-5 0.0 0.001193144430729912 0.0 28-29 5.369149938284604E-4 5.9657221536495605E-5 0.0 0.0020283455322408508 0.0 30-31 5.369149938284604E-4 1.1931444307299121E-4 0.0 0.00447429161523717 0.0 32-33 5.369149938284604E-4 1.1931444307299121E-4 0.0 0.008709954344328358 0.0 34-35 6.264008261332038E-4 1.1931444307299121E-4 0.0 0.014944133994892148 0.0 36-37 7.158866584379473E-4 1.1931444307299121E-4 0.0 0.024668261105340934 0.0 38-39 7.158866584379473E-4 1.1931444307299121E-4 0.0 0.0454289742000414 0.0 40-41 7.158866584379473E-4 1.1931444307299121E-4 0.0 0.07340821110065784 0.0 42-43 7.755438799744429E-4 1.1931444307299121E-4 0.0 0.09894150191827797 0.0 44-45 7.755438799744429E-4 1.4914305384123902E-4 0.0 0.12745765381272287 0.0 46-47 8.650297122791863E-4 1.7897166460948682E-4 0.0 0.1641170164468994 0.0 48-49 8.948583230474341E-4 1.7897166460948682E-4 0.0 0.20563844263630035 0.0 50-51 8.948583230474341E-4 1.7897166460948682E-4 0.0 0.25935977062991467 0.0 52-53 9.246869338156819E-4 1.7897166460948682E-4 0.0 0.30854714978675524 0.0 54-55 0.0010141727661204252 1.7897166460948682E-4 0.0 0.36277556416342976 0.0 56-57 0.0011931444307299122 1.7897166460948682E-4 0.0 0.42001666822769734 0.0 58-59 0.0011931444307299122 1.7897166460948682E-4 0.0 0.492082591843784 0.0 60-61 0.0011931444307299122 1.7897166460948682E-4 0.0 0.5752447586656588 0.0 62-63 0.0012528016522664078 1.7897166460948682E-4 0.0 0.6627619026596979 0.0 64-65 0.0012528016522664078 1.7897166460948682E-4 0.0 0.7440746956139415 0.0 66-67 0.001372116095339399 1.7897166460948682E-4 0.0 0.8369908181570334 0.0 68-69 0.001372116095339399 1.7897166460948682E-4 0.0 0.9494745093640957 0.0 70-71 0.0014616019276441424 1.7897166460948682E-4 0.0 1.0824802847797126 0.0 72-73 0.001521259149180638 1.7897166460948682E-4 0.0 1.217574062949107 0.0 74-75 0.0015510877599488858 1.7897166460948682E-4 0.0 1.3501324092032003 0.0 76-77 0.0016107449814853814 1.7897166460948682E-4 0.0 1.4973962605660396 0.0 78-79 0.0016107449814853814 1.7897166460948682E-4 0.0 1.667031570005065 0.0 80-81 0.0017300594245583726 1.7897166460948682E-4 0.0 1.8668534335415568 0.0 82-83 0.0017300594245583726 1.7897166460948682E-4 0.0 2.0849005782574483 0.0 84-85 0.0017598880353266204 1.7897166460948682E-4 0.0 2.303216180470254 0.0 86-87 0.0017897166460948682 1.7897166460948682E-4 0.0 2.521680925736901 0.0 88-89 0.0017897166460948682 1.7897166460948682E-4 0.0 2.7662457054257645 0.0 90-91 0.0019686883107043547 1.7897166460948682E-4 0.0 3.0526003688009435 0.0 92-93 0.0019686883107043547 1.7897166460948682E-4 0.0 3.372959648451925 0.0 94-95 0.0019686883107043547 1.7897166460948682E-4 0.0 3.6819243987894357 0.0 96-97 0.0019686883107043547 1.7897166460948682E-4 0.0 3.995065154634501 0.0 98-99 0.002088002753777346 1.7897166460948682E-4 0.0 4.33487268850638 0.0 100-101 0.0021774885860820895 1.7897166460948682E-4 0.0 4.737230819159275 0.0 102-103 0.002266974418386833 1.7897166460948682E-4 0.0 5.175771054674054 0.0 104-105 0.0025056033045328155 1.7897166460948682E-4 0.0 5.601097215618499 0.0 106-107 0.0025354319153010636 1.7897166460948682E-4 0.0 6.022128056612317 0.0 108-109 0.002565260526069311 1.7897166460948682E-4 0.0 6.465112755131565 0.0 110-111 0.002565260526069311 1.7897166460948682E-4 0.0 6.9710059937610485 0.0 112-113 0.002565260526069311 1.7897166460948682E-4 0.0 7.531425932874887 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6410 0.0 53.10112 1 GTATCAA 10660 0.0 49.489334 1 TATCAAC 14190 0.0 37.068535 2 ATCAACG 14250 0.0 36.912457 3 TCAACGC 14255 0.0 36.774147 4 CAACGCA 14440 0.0 36.137154 5 AACGCAG 14630 0.0 35.70851 6 TAACGCA 145 1.08704626E-7 32.866173 4 ACGCAGA 16805 0.0 30.87447 7 CGCAGAG 16990 0.0 30.573305 8 GTACATG 12090 0.0 29.830095 1 TACATGG 12305 0.0 28.707819 2 GCAGAGT 17535 0.0 28.603384 9 GAGTACT 9570 0.0 27.883427 12-13 ACATGGG 12610 0.0 27.541058 3 AGTACTT 9690 0.0 27.507421 12-13 TATAACG 110 7.6996995E-4 27.077244 2 GTACTTT 10445 0.0 25.917826 14-15 ACTTGAC 1105 0.0 25.307959 8 CATGGGG 4760 0.0 25.279678 4 >>END_MODULE