FastQCFastQC Report
Thu 26 May 2016
SRR1364536_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364536_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984890
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25750.12973011098851825No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28250.055.5997621
GTATCAA50500.052.309321
ATCAACG60300.043.510873
TCAACGC60800.043.348764
CAACGCA61300.042.8981255
AACGCAG64550.041.286076
TATCAAC65850.039.662972
GTACATG92950.037.637031
TACATGG94750.035.665282
ACGCAGA75500.034.510327
ACATGGG98350.033.5733873
CGCAGAG76900.033.268228
GCAGAGT87250.028.2314579
CATGGGA58300.027.9613744
CATGGGG41250.027.403514
GAGTACT39700.023.98607412-13
CAGAGTA84700.023.36026410-11
GAGTACA70700.023.3102971
AGTACAT69150.022.5416452
AGAGTAC81800.022.11514510-11
Produced by FastQC (version 0.11.5)