##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364536_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1984890 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.8506813979616 31.0 30.0 33.0 23.0 34.0 2 28.846940132702567 31.0 30.0 34.0 22.0 34.0 3 29.001154220133106 31.0 30.0 34.0 22.0 34.0 4 32.38413211815264 35.0 33.0 37.0 23.0 37.0 5 32.484763891198 35.0 33.0 37.0 25.0 37.0 6 32.61197547471144 35.0 33.0 37.0 25.0 37.0 7 32.47246144622624 35.0 33.0 37.0 23.0 37.0 8 32.414727768289424 35.0 33.0 37.0 23.0 37.0 9 33.611220269133305 38.0 33.0 39.0 21.0 39.0 10-11 33.53283557275214 38.0 33.0 39.0 19.0 39.0 12-13 33.458501226768234 38.0 33.0 39.0 18.5 39.0 14-15 34.24301724528816 39.0 33.0 41.0 17.0 41.0 16-17 34.35392263551128 39.0 33.0 41.0 18.0 41.0 18-19 34.16129634387799 39.0 33.0 41.0 17.5 41.0 20-21 33.8937349173002 39.0 32.5 41.0 17.0 41.0 22-23 33.8050894508008 39.0 32.5 41.0 16.5 41.0 24-25 33.63646700824731 38.5 32.0 41.0 16.0 41.0 26-27 33.56151046153691 38.0 32.0 41.0 14.5 41.0 28-29 33.623138561834665 38.0 32.0 40.5 15.5 41.0 30-31 33.5753943543471 38.0 32.5 40.0 15.5 41.0 32-33 33.56965650489448 38.0 32.5 40.0 15.5 41.0 34-35 33.113574555768835 38.0 31.5 40.0 9.0 41.0 36-37 32.97995153383815 38.0 31.5 40.0 9.0 41.0 38-39 32.79325453803486 38.0 31.0 40.0 9.0 41.0 40-41 33.01191023180125 38.0 31.5 40.0 9.0 41.0 42-43 32.90701701353727 38.0 32.0 40.0 9.0 41.0 44-45 32.76311558826937 38.0 31.5 40.0 8.0 41.0 46-47 32.73642897087496 38.0 32.0 40.0 7.5 41.0 48-49 32.604480349036976 38.0 31.0 40.0 2.0 41.0 50-51 32.462473991002014 38.0 31.0 40.0 2.0 41.0 52-53 32.4163643829129 38.0 31.0 40.0 2.0 41.0 54-55 32.45365158774541 38.0 31.0 40.0 2.0 41.0 56-57 32.33408954652399 37.5 31.0 40.0 2.0 41.0 58-59 32.152508703253076 37.0 31.0 40.0 2.0 41.0 60-61 31.955273591987467 37.0 31.0 40.0 2.0 41.0 62-63 31.68382227730504 36.0 31.0 40.0 2.0 41.0 64-65 31.41144068437042 36.0 30.0 39.5 2.0 41.0 66-67 31.163993470670917 35.5 30.0 39.0 2.0 41.0 68-69 31.120261072402 35.0 30.0 39.0 2.0 41.0 70-71 30.87546211628856 35.0 30.0 39.0 2.0 40.5 72-73 30.58789026092126 35.0 30.0 38.0 2.0 40.0 74-75 30.281660192756274 35.0 30.0 37.0 2.0 39.5 76-77 29.96021416803954 35.0 30.0 37.0 2.0 39.0 78-79 29.63816962149036 35.0 29.5 36.0 2.0 39.0 80-81 29.329218999541535 34.5 29.0 36.0 2.0 38.5 82-83 29.071620593584534 34.0 29.0 35.5 2.0 37.0 84-85 28.810404858707535 34.0 29.0 35.0 2.0 37.0 86-87 28.60901838389029 34.0 29.0 35.0 2.0 36.5 88-89 28.40670087511147 34.0 29.0 35.0 2.0 36.0 90-91 28.265008892180425 34.0 29.0 35.0 2.0 36.0 92-93 28.143083999617108 34.0 29.0 35.0 2.0 36.0 94-95 28.01853881071495 34.0 29.0 35.0 2.0 35.0 96-97 27.90340044032667 34.0 28.5 35.0 2.0 35.0 98-99 27.81479225549023 34.0 28.0 35.0 2.0 35.0 100-101 27.72607172185864 34.0 28.0 35.0 2.0 35.0 102-103 26.540492672138 32.5 25.5 34.0 2.0 35.0 104-105 27.431614598290082 34.0 27.0 35.0 2.0 35.0 106-107 27.58166472701258 34.0 27.5 35.0 2.0 35.0 108-109 27.54152497115709 34.0 27.5 35.0 2.0 35.0 110-111 27.465513454146073 34.0 27.0 35.0 2.0 35.0 112-113 27.385988140400727 34.0 27.0 35.0 2.0 35.0 114-115 27.269398304188144 34.0 27.0 35.0 2.0 35.0 116-117 27.17044546549179 34.0 27.0 35.0 2.0 35.0 118-119 27.0324922287885 34.0 27.0 35.0 2.0 35.0 120-121 26.865854782884696 34.0 26.5 35.0 2.0 35.0 122-123 26.64818982412124 34.0 25.5 35.0 2.0 35.0 124-125 25.212822624931356 32.0 21.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5943473234683623 1101 2 0.7131899474410339 1101 3 0.666475244683042 1101 4 0.8119132331035317 1101 5 0.6642350004391062 1101 6 0.7153629936641437 1101 7 0.8453064973939775 1101 8 0.6909084514233541 1101 9 0.9702677660399317 1101 10-11 1.0707748043119736 1101 12-13 0.8633101114896249 1101 14-15 1.2396175711950264 1101 16-17 0.9734755346437964 1101 18-19 1.3594693618581886 1101 20-21 1.6578164989055182 1101 22-23 1.537973459157925 1101 24-25 1.6920851579312526 1101 26-27 1.7364015972373892 1101 28-29 1.5120860274306551 1101 30-31 1.6063753689232243 1101 32-33 1.6577535403768238 1101 34-35 1.971743727533422 1101 36-37 1.819104383051446 1101 38-39 1.806575833882512 1101 40-41 1.5897665853204614 1101 42-43 1.6219647153082093 1101 44-45 1.5060034798147655 1101 46-47 1.454503992005094 1101 48-49 1.3670063769710552 1101 50-51 1.3971855467064884 1101 52-53 1.2738038947735077 1101 54-55 1.2139246524445966 1101 56-57 1.2248393380519147 1101 58-59 1.2084759264651055 1101 60-61 1.2876738086900872 1101 62-63 1.262791888402841 1101 64-65 1.3439569379958698 1101 66-67 1.3028141721861068 1101 68-69 1.0641022386057521 1101 70-71 0.9226789938920383 1101 72-73 0.8221015670649159 1101 74-75 0.7588747537803258 1101 76-77 0.9086839444579837 1101 78-79 1.0418231051159097 1101 80-81 1.0728356964992152 1101 82-83 0.9120340240045692 1101 84-85 0.9392526134977217 1101 86-87 1.0011353557894829 1101 88-89 0.9881042074934818 1101 90-91 0.9654325507994805 1101 92-93 0.8800396044373286 1101 94-95 0.9310201404295029 1101 96-97 0.8607277231472601 1101 98-99 0.934425757061927 1101 100-101 0.9931276445721906 1101 102-103 0.9664787685451657 1101 104-105 0.9332159922734284 1101 106-107 0.8960334449894809 1101 108-109 0.9108552453633436 1101 110-111 0.7137467141810347 1101 112-113 0.7699749398984537 1101 114-115 0.8713136025050972 1101 116-117 1.0111656677489052 1101 118-119 1.07177212703699 1101 120-121 1.1248166194016989 1101 122-123 1.1603288547935406 1101 124-125 1.3635432622555221 1102 1 0.22359455495661962 1102 2 0.5118008389741107 1102 3 0.529569912790766 1102 4 0.7190060130589586 1102 5 0.7546080680508211 1102 6 0.03410875643322697 1102 7 -0.4060673359433906 1102 8 0.47776868933279104 1102 9 0.7622080113084451 1102 10-11 0.7678547509080929 1102 12-13 0.9372483211994265 1102 14-15 1.005379338811636 1102 16-17 0.8946209274340475 1102 18-19 1.158559943095156 1102 20-21 1.4449561901059766 1102 22-23 1.2562487029270457 1102 24-25 1.6146978070888096 1102 26-27 1.0439221370824114 1102 28-29 1.5419249662395487 1102 30-31 1.430695738181896 1102 32-33 1.193304027352454 1102 34-35 1.5847096963451577 1102 36-37 1.7517316659519153 1102 38-39 1.7284467812612405 1102 40-41 1.4689645594388168 1102 42-43 1.2009093979596557 1102 44-45 1.286419469696611 1102 46-47 0.9965222445190207 1102 48-49 0.8288729584195735 1102 50-51 1.2015892849779775 1102 52-53 1.0460490124989121 1102 54-55 0.7772353103216432 1102 56-57 0.8982979143198193 1102 58-59 1.004729215681536 1102 60-61 0.9375530985962968 1102 62-63 0.7670586849971919 1102 64-65 0.8217213566773296 1102 66-67 0.8310746246787666 1102 68-69 0.7106477807153162 1102 70-71 0.819665826899147 1102 72-73 0.9642513038432092 1102 74-75 0.6996276179374448 1102 76-77 0.8300712637937124 1102 78-79 0.7325141654799552 1102 80-81 0.43697025491857033 1102 82-83 0.6172111395537492 1102 84-85 0.20126423834954466 1102 86-87 0.6524452192472623 1102 88-89 0.7899410488554928 1102 90-91 0.2940789042631664 1102 92-93 0.6464439402111211 1102 94-95 0.6392660098150493 1102 96-97 0.8352504386433317 1102 98-99 0.7485803728797613 1102 100-101 0.8198309340418035 1102 102-103 0.6826656798689612 1102 104-105 0.9866396748568 1102 106-107 0.9408822943474462 1102 108-109 0.5020910342886253 1102 110-111 0.7349776814539979 1102 112-113 0.42476021883694415 1102 114-115 0.4977197161576825 1102 116-117 0.41799514160367934 1102 118-119 0.6667887777721369 1102 120-121 1.063411780083122 1102 122-123 0.9534475636484459 1102 124-125 1.1727020949093045 1103 1 0.7323513065124665 1103 2 0.6682953196040309 1103 3 0.6026086809618647 1103 4 0.7732768450277163 1103 5 0.6907791289976615 1103 6 0.7685364173830465 1103 7 0.9171934537848045 1103 8 0.8986959263411407 1103 9 1.1052341402869317 1103 10-11 1.2330339559579926 1103 12-13 1.1492205231066919 1103 14-15 1.440872703235513 1103 16-17 1.3165605872599784 1103 18-19 1.3391489010122513 1103 20-21 1.7422700454707893 1103 22-23 1.6842337635640021 1103 24-25 1.8381049097007178 1103 26-27 1.7076966887592206 1103 28-29 1.662326149932916 1103 30-31 1.6516799147981231 1103 32-33 1.6558625084630094 1103 34-35 2.08967344788158 1103 36-37 2.003325173165244 1103 38-39 2.1441823727849325 1103 40-41 1.853178228197109 1103 42-43 1.6778621261144764 1103 44-45 1.4341678707122583 1103 46-47 1.5296856700022161 1103 48-49 1.5716606457319102 1103 50-51 1.5147019617087167 1103 52-53 1.4657165086784687 1103 54-55 1.255673922829203 1103 56-57 1.2885420205848064 1103 58-59 1.2445525312426113 1103 60-61 1.235760517323527 1103 62-63 1.2743406193989664 1103 64-65 1.4189078489128946 1103 66-67 1.4517638378641813 1103 68-69 1.4533337175582055 1103 70-71 1.4093835410573448 1103 72-73 1.4347117083425438 1103 74-75 1.4399364221424378 1103 76-77 1.4253166484330713 1103 78-79 1.4786254003128114 1103 80-81 1.5717240142652678 1103 82-83 1.5534810712397729 1103 84-85 1.4719491149999655 1103 86-87 1.4825309697094902 1103 88-89 1.452266757415952 1103 90-91 1.5226523559056098 1103 92-93 1.5189602575533279 1103 94-95 1.5039609797506657 1103 96-97 1.5959833627462885 1103 98-99 1.5304807260814997 1103 100-101 1.5566878451008286 1103 102-103 1.524015636435852 1103 104-105 1.4826901530275443 1103 106-107 1.4848216704917157 1103 108-109 1.6227485139757967 1103 110-111 1.658220616854532 1103 112-113 1.767733778621725 1103 114-115 1.832227885331335 1103 116-117 1.8545643958416242 1103 118-119 1.9030704768265458 1103 120-121 1.9347179895946809 1103 122-123 1.9055091456073754 1103 124-125 1.9091095986506943 1104 1 0.7411036137878604 1104 2 0.7855905537062 1104 3 0.7384562840372091 1104 4 0.8387280483900739 1104 5 0.8698944305583396 1104 6 0.8356042048358958 1104 7 0.8209611088089943 1104 8 0.8621250787125021 1104 9 1.1041761597132123 1104 10-11 1.038435196049278 1104 12-13 1.0936771821207856 1104 14-15 1.2649684131945946 1104 16-17 1.2253716522966016 1104 18-19 1.4897549709148095 1104 20-21 1.7329337823450928 1104 22-23 1.5496090377526883 1104 24-25 1.9709426155659244 1104 26-27 2.055127410844811 1104 28-29 1.9406440673431078 1104 30-31 1.8095706262144233 1104 32-33 1.8045650229031835 1104 34-35 2.082347901762482 1104 36-37 2.144677002197625 1104 38-39 2.1835492206249185 1104 40-41 1.8410657780282946 1104 42-43 1.7751040904899753 1104 44-45 1.7443999020860943 1104 46-47 1.7269736946676986 1104 48-49 1.6953414722094564 1104 50-51 1.5330074117360724 1104 52-53 1.5824120216136919 1104 54-55 1.4658713983333058 1104 56-57 1.4395275233109288 1104 58-59 1.354104438334609 1104 60-61 1.2885344887005274 1104 62-63 1.3188591040196904 1104 64-65 1.3871456359055685 1104 66-67 1.3262435824149215 1104 68-69 1.3116320689321732 1104 70-71 1.3069841876909791 1104 72-73 1.2956373284307965 1104 74-75 1.3484603027623479 1104 76-77 1.413259802962152 1104 78-79 1.4018732344991776 1104 80-81 1.3081892768813574 1104 82-83 1.279520772567981 1104 84-85 1.1915278060866825 1104 86-87 1.290335355364924 1104 88-89 1.3024270097291897 1104 90-91 1.3374955293530668 1104 92-93 1.407929606369052 1104 94-95 1.3882944732937048 1104 96-97 1.1879908159062538 1104 98-99 1.3531603271252486 1104 100-101 1.3953549685355888 1104 102-103 1.3609745665077249 1104 104-105 1.3967998557703645 1104 106-107 1.4239526351075291 1104 108-109 1.4252229443365572 1104 110-111 1.5476584512283758 1104 112-113 1.48141347170084 1104 114-115 1.4872294959501708 1104 116-117 1.5334547220728965 1104 118-119 1.5694819157994324 1104 120-121 1.5412960040510484 1104 122-123 1.5488580567779167 1104 124-125 1.7087586286150227 1105 1 0.1762142496647705 1105 2 0.44942105425988643 1105 3 0.48722911305787875 1105 4 0.588363579879136 1105 5 0.6320962944697754 1105 6 0.42059860023301354 1105 7 0.682079133956897 1105 8 0.5638746968883481 1105 9 0.6195515665125555 1105 10-11 0.7487580787454462 1105 12-13 0.7927754082893728 1105 14-15 0.9404665624903785 1105 16-17 0.8449994457085666 1105 18-19 0.8762685354083857 1105 20-21 1.2991739234930435 1105 22-23 1.422780980534938 1105 24-25 1.4555447734226945 1105 26-27 1.3565178941952425 1105 28-29 1.111303378603452 1105 30-31 1.1279258276584159 1105 32-33 1.2066292438633823 1105 34-35 1.7303242337446108 1105 36-37 1.715780956575145 1105 38-39 1.8632436258427916 1105 40-41 1.3868997039933504 1105 42-43 1.2578872922590634 1105 44-45 1.3246305966843437 1105 46-47 1.2611537294128468 1105 48-49 1.089926257490518 1105 50-51 1.0732780073817523 1105 52-53 1.1075880755668877 1105 54-55 1.030437143203656 1105 56-57 1.0268154034841785 1105 58-59 0.9382304166002697 1105 60-61 0.8993138671483791 1105 62-63 1.012884190967224 1105 64-65 1.0025477534633893 1105 66-67 0.8689302106113281 1105 68-69 0.6628624570551587 1105 70-71 0.8181140231425488 1105 72-73 0.8825078265462274 1105 74-75 0.8525323176763493 1105 76-77 0.9201463952620017 1105 78-79 0.9887096562904141 1105 80-81 0.9283263102685311 1105 82-83 0.9340467433834085 1105 84-85 0.8819818635821193 1105 86-87 0.8736485815504444 1105 88-89 1.0267436206884746 1105 90-91 0.8779030652580779 1105 92-93 0.8672111504625519 1105 94-95 0.9109101465402745 1105 96-97 1.0635504295780969 1105 98-99 0.9600897159018196 1105 100-101 0.9487324613986097 1105 102-103 0.9241757768310315 1105 104-105 1.0585900099562089 1105 106-107 0.9760553347990211 1105 108-109 0.9400349276886857 1105 110-111 1.0355095008638244 1105 112-113 1.058201822593002 1105 114-115 1.085332481798126 1105 116-117 1.1422108981420855 1105 118-119 1.1813362858657719 1105 120-121 0.9463346013086849 1105 122-123 1.1928929644797535 1105 124-125 1.4110054224773876 1106 1 0.43049856830637623 1106 2 0.7099366768283168 1106 3 0.5380482820444428 1106 4 0.6458891275768153 1106 5 0.5230854356175669 1106 6 0.5253899823159074 1106 7 0.25893793429580825 1106 8 0.5756067529808888 1106 9 0.7506505930199125 1106 10-11 0.7487576464662951 1106 12-13 0.773285886735934 1106 14-15 1.0693804179016482 1106 16-17 0.821572509530256 1106 18-19 0.8796848375196333 1106 20-21 1.2655732975528338 1106 22-23 1.2793362776836261 1106 24-25 1.487839051775886 1106 26-27 1.6183636397038654 1106 28-29 1.4280879133846298 1106 30-31 1.2165272749290068 1106 32-33 1.2074985572311832 1106 34-35 1.5297579480599737 1106 36-37 1.5099949001713568 1106 38-39 1.5702603809825888 1106 40-41 1.313844744022397 1106 42-43 1.2491414205249285 1106 44-45 1.1497462313565876 1106 46-47 0.8386056167436138 1106 48-49 1.0376675989391728 1106 50-51 0.9092475230561874 1106 52-53 0.9227915493621239 1106 54-55 0.6742337212819223 1106 56-57 0.7433487840800339 1106 58-59 0.6873621839303397 1106 60-61 0.8211072204241887 1106 62-63 0.8501760485571843 1106 64-65 0.8191306300217533 1106 66-67 0.7028773082891213 1106 68-69 0.7812823038554235 1106 70-71 0.8558669064444722 1106 72-73 0.8137326462858177 1106 74-75 0.7928395384672484 1106 76-77 0.6468100215653223 1106 78-79 0.6631029525053762 1106 80-81 0.659652715608047 1106 82-83 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90-91 -2.3314941906600453 2216 92-93 -2.3201149003426664 2216 94-95 -2.3736278783685805 2216 96-97 -2.3159341317519555 2216 98-99 -2.273807812567977 2216 100-101 -2.2820012951139965 2216 102-103 -2.251292004795829 2216 104-105 -2.398604564290423 2216 106-107 -2.3786423797169256 2216 108-109 -2.3881483968887594 2216 110-111 -2.4602222016361495 2216 112-113 -2.531275954570326 2216 114-115 -2.6273597362590166 2216 116-117 -2.573112480471689 2216 118-119 -2.6268950216317677 2216 120-121 -2.8255187599644884 2216 122-123 -2.852425960481632 2216 124-125 -3.028135516364383 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 103150.0 3 17601.0 4 11450.0 5 8959.0 6 8249.0 7 8979.0 8 9413.0 9 9680.0 10 10333.0 11 11432.0 12 12153.0 13 13612.0 14 15472.0 15 13858.0 16 10800.0 17 9792.0 18 8795.0 19 8243.0 20 8462.0 21 8952.0 22 10809.0 23 14279.0 24 18890.0 25 24123.0 26 29600.0 27 34173.0 28 39492.0 29 46754.0 30 53713.0 31 62246.0 32 76935.0 33 98310.0 34 128906.0 35 178249.0 36 281229.0 37 414697.0 38 162501.0 39 599.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.67053902268694 18.9711518210117 12.990044672504089 23.368264483797276 2 14.827438532849657 20.20646916565901 41.59184804514309 23.37424425634825 3 19.499140697387933 25.65598974983738 28.39457281500079 26.450296737773893 4 11.9020147645948 16.81044646474011 38.46639472618186 32.82114404448323 5 13.394752064366722 38.6141886539946 34.088504243616434 13.902555038022237 6 31.191375135651473 37.1529362687456 18.2803776174311 13.375310978171825 7 28.56541851008204 32.50144727315599 20.965483605884216 17.96765061087775 8 25.57784058562439 36.2693146723496 19.96065273138562 18.192192010640387 9 26.44087932218453 16.85641261014073 19.85996905554937 36.84273901212537 10-11 24.607509736055903 27.33325272433233 27.721914060728807 20.33732347888296 12-13 25.488258838839627 24.537650135389192 27.98768763223716 21.98640339353402 14-15 22.335370557649213 26.022907813962064 25.86332608236675 25.77839554602197 16-17 22.161276456142986 29.554583850080302 26.168419280889793 22.11572041288692 18-19 21.998846228451804 28.098065543292883 28.40159814993488 21.501490078320433 20-21 22.881742566634948 27.302877616103384 28.35915647835836 21.456223338903303 22-23 22.36685359052767 27.3913024859001 28.059739391476807 22.182104532095426 24-25 22.225981549791328 27.41164502520037 28.292461352964914 22.069912072043387 26-27 22.05456014507852 27.803533290788657 27.991683884831076 22.150222679301745 28-29 21.962394228572336 27.70339186495516 28.298364411175175 22.035849495297327 30-31 22.167071480500166 27.486330290805967 28.165419981127158 22.181178247566706 32-33 21.96197477245073 27.58899802744447 28.054828478361095 22.394198721743706 34-35 22.44718939666474 27.51071428211539 28.095956435471958 21.946139885747915 36-37 22.426187448874682 27.29247372344475 27.977638003402518 22.303700824278046 38-39 22.09843419495141 27.527854825167847 28.180233710743092 22.193477269137652 40-41 22.40238332018533 27.40059819845061 28.02976227520001 22.167256206164048 42-43 22.27414651989921 27.386512099648968 27.872271602089356 22.467069778362465 44-45 22.33541595126276 27.64076314564914 27.72914360893106 22.29467729415704 46-47 22.3980733793576 27.832413225867803 27.308714003393252 22.460799391381343 48-49 22.425570019688084 27.734474405741842 27.349354909095673 22.490600665474396 50-51 22.435283804072913 27.812547233502283 27.25249644804063 22.499672514384176 52-53 22.658887898069917 27.739773992513438 27.19458005229509 22.406758057121554 54-55 22.418167306426792 27.716897830190295 27.35108067225705 22.51385419112586 56-57 22.378517461603415 28.016809652501095 27.206380251630886 22.398292634264607 58-59 22.46329132418251 27.6028334135227 27.36367598122813 22.57019928106666 60-61 22.552756056659057 27.667301737009335 27.525198135145285 22.254744071186323 62-63 22.389652313104406 27.788369888081977 27.463467717775792 22.35851008103783 64-65 22.22078896788304 27.544416258042215 27.45918521338372 22.77560956069103 66-67 22.227050453081183 27.96072154425464 27.243666284774598 22.568561717889576 68-69 22.300863729653273 27.632445517408883 27.548118373441007 22.51857237949684 70-71 22.23312150271017 27.612596578919064 27.60153775580456 22.55274416256621 72-73 22.313190946481736 27.69542502167848 27.410493443046686 22.580890588793096 74-75 22.30351420154981 27.78014286945013 27.288906331789835 22.62743659721022 76-77 22.386874567963932 27.658646666545746 27.393908064740412 22.560570700749906 78-79 22.41884598875089 27.71753587644115 27.307130751264065 22.556487383543892 80-81 22.22242934810264 27.939649452322783 27.335821530936798 22.502099668637776 82-83 22.50575984337458 27.898902846191397 27.192430913367826 22.402906397066197 84-85 22.494067681332467 27.789776763447847 27.129060048667686 22.587095506552 86-87 22.380083393960888 27.685842776789148 27.378859086777684 22.555214742472273 88-89 22.468656120786175 27.628794345252793 27.328002950723523 22.574546583237513 90-91 22.33569554592709 27.951919605323127 27.3417940302125 22.370590818537277 92-93 22.394060495402083 27.825254986366467 27.377404006021727 22.40328051220972 94-95 22.292739266776152 27.697484818356948 27.356738754800404 22.653037160066496 96-97 22.494577624060863 27.637829376743582 27.315371531953158 22.5522214672424 98-99 22.204336951448038 27.647127134298483 27.19863507674419 22.949900837509293 100-101 22.191745276780235 27.583049186481368 27.445424280479706 22.779781256258683 102-103 22.414466292842423 27.51467335721375 27.41212359375079 22.658736756193036 104-105 22.266734076809776 27.507167365832778 27.46220207978933 22.763896477568117 106-107 22.25157735560461 27.594094076317134 27.449199287516652 22.705129280561604 108-109 22.320254522920667 27.615585750343847 27.08049312556363 22.983666601171855 110-111 22.092549908684425 27.698671200957236 27.409736129479185 22.79904276087915 112-113 22.275818811117997 27.60135322360433 27.42877439052038 22.69405357475729 114-115 22.08495181093159 27.82204555416169 27.296424486999282 22.79657814790744 116-117 22.017139488838172 27.695035996957014 27.42073868073294 22.867085833471883 118-119 22.069988765120485 27.792069081913862 27.1832444115291 22.954697741436554 120-121 22.034128860606312 27.82561140698548 27.301879349424578 22.838380382983633 122-123 22.131201225256817 27.89721344759659 27.194252578228518 22.777332748918074 124-125 22.147117997508097 27.81618376080759 27.15196196384096 22.884736277843356 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 754.0 1 724.5 2 859.5 3 983.0 4 788.5 5 583.5 6 508.0 7 576.0 8 797.5 9 889.0 10 791.5 11 743.5 12 768.5 13 846.0 14 973.5 15 1119.5 16 1284.5 17 1421.0 18 1578.0 19 1817.5 20 2136.5 21 2657.0 22 3328.0 23 4258.5 24 5451.0 25 7123.0 26 9655.5 27 12744.0 28 15776.0 29 19364.5 30 23793.0 31 28197.5 32 32886.0 33 37890.5 34 43047.5 35 47245.5 36 51825.0 37 57109.0 38 60486.0 39 62419.5 40 64048.5 41 66676.0 42 68965.0 43 70820.5 44 73229.5 45 74522.5 46 73918.5 47 73102.0 48 74771.5 49 75258.5 50 73298.5 51 70770.0 52 67923.0 53 66415.0 54 64047.0 55 58692.0 56 52419.5 57 46874.5 58 42506.5 59 37775.5 60 31967.0 61 25995.5 62 20636.0 63 16032.0 64 11877.0 65 8806.5 66 6559.5 67 5115.5 68 3998.0 69 2901.0 70 2103.5 71 1619.5 72 1322.5 73 1128.0 74 848.5 75 609.5 76 459.0 77 297.0 78 190.5 79 141.0 80 103.0 81 73.5 82 50.0 83 32.5 84 33.5 85 24.0 86 14.5 87 13.0 88 12.5 89 10.5 90 8.0 91 6.0 92 5.5 93 6.5 94 6.0 95 4.0 96 5.0 97 5.0 98 4.5 99 4.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011285260140360423 2 0.004534256306394813 3 0.007103668213351874 4 0.007154048838978483 5 0.013199723914171567 6 0.0012091350150386167 7 0.006196816952072911 8 0.0 9 0.002166366901944188 10-11 0.0 12-13 0.0028465053479034105 14-15 0.016474464579901153 16-17 0.026248305951463307 18-19 0.004786159434527858 20-21 0.00702809727491196 22-23 0.0017885122097446207 24-25 0.009924983248441979 26-27 0.015592803631435495 28-29 4.282353178261768E-4 30-31 0.001360276891918444 32-33 0.0078089969721243995 34-35 0.00785937759775101 36-37 0.009244844802482758 38-39 2.5190312813304517E-5 40-41 0.021739239957881795 42-43 0.008212041977137272 44-45 0.014534810493276705 46-47 0.0034006922297961095 48-49 0.02221785590133458 50-51 0.0035266437938626318 52-53 0.0 54-55 0.014711142682969839 56-57 0.003929688798875505 58-59 2.519031281330451E-4 60-61 0.003551834106675937 62-63 0.022494949342280933 64-65 0.01433328799077027 66-67 0.001813702522557925 68-69 0.017985883348699425 70-71 0.002493840968517147 72-73 0.010227267002201633 74-75 5.038062562660903E-5 76-77 0.005138823813914121 78-79 0.0011083737637853986 80-81 0.0026701731582102787 82-83 0.001360276891918444 84-85 0.0 86-87 0.0047357788089012485 88-89 0.014711142682969839 90-91 0.019169828050924737 92-93 0.003929688798875505 94-95 0.0212102433888024 96-97 0.014585191118903315 98-99 8.31280322839049E-4 100-101 0.0074311422799248315 102-103 0.0 104-105 0.0012847059534785302 106-107 7.557093843991354E-5 108-109 0.0 110-111 7.557093843991355E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 5.038062562660903E-5 122-123 0.0 124-125 0.023301039352306676 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1984890.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.138548131116764 #Duplication Level Percentage of deduplicated Percentage of total 1 79.78287216525403 49.57591842079374 2 11.873083499128173 14.755523409506885 3 3.658989616968486 6.820929072757583 4 1.6027578375273608 3.9837218011887408 5 0.8358084652783171 2.596796227404577 6 0.5122102114662955 1.9096799327068745 7 0.3225600821179396 1.4030390631503795 8 0.2312001749156498 1.1493154557534975 9 0.1796495033719104 1.0046843380806012 >10 0.9047943989864483 10.200554949855645 >50 0.06515388717121595 2.7861570942935083 >100 0.02993122642968714 3.233226593210455 >500 7.416985384135378E-4 0.3037203834614443 >1k 2.472328461378445E-4 0.27673325783605424 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2575 0.12973011098851825 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.038062562660903E-5 0.0 0.0 0.0 0.0 8 5.038062562660903E-5 5.038062562660903E-5 0.0 0.0 0.0 9 5.038062562660903E-5 5.038062562660903E-5 0.0 0.0 0.0 10-11 5.038062562660903E-5 5.038062562660903E-5 0.0 0.0 0.0 12-13 5.038062562660903E-5 5.038062562660903E-5 0.0 0.0 0.0 14-15 1.763321896931316E-4 5.038062562660903E-5 0.0 5.038062562660903E-5 0.0 16-17 2.0152250250643613E-4 5.038062562660903E-5 0.0 1.2595156406652256E-4 5.038062562660903E-5 18-19 3.0228375375965417E-4 5.038062562660903E-5 0.0 1.5114187687982709E-4 5.038062562660903E-5 20-21 4.2823531782617674E-4 5.038062562660903E-5 0.0 3.0228375375965417E-4 5.038062562660903E-5 22-23 5.541868818926993E-4 5.038062562660903E-5 0.0 5.793771947060039E-4 5.038062562660903E-5 24-25 6.045675075193083E-4 5.038062562660903E-5 0.0 0.0010579931381587897 5.038062562660903E-5 26-27 6.045675075193083E-4 5.038062562660903E-5 0.0 0.0014358478303583575 5.038062562660903E-5 28-29 7.8089969721244E-4 5.038062562660903E-5 0.0 0.001763321896931316 5.038062562660903E-5 30-31 8.060900100257445E-4 5.038062562660903E-5 0.0 0.0034006922297961095 5.038062562660903E-5 32-33 8.060900100257445E-4 5.038062562660903E-5 0.0 0.006297578203326129 5.038062562660903E-5 34-35 9.824221997188761E-4 5.038062562660903E-5 0.0 0.009849412310002066 5.038062562660903E-5 36-37 0.0011083737637853986 5.038062562660903E-5 0.0 0.01690269989772733 5.038062562660903E-5 38-39 0.0011083737637853986 5.038062562660903E-5 0.0 0.029699378806886025 5.038062562660903E-5 40-41 0.0011335640765987032 5.038062562660903E-5 0.0 0.045518895253641264 5.038062562660903E-5 42-43 0.0012091350150386167 5.038062562660903E-5 0.0 0.061917788895102494 5.038062562660903E-5 44-45 0.0012091350150386167 5.038062562660903E-5 0.0 0.08078533319226758 5.038062562660903E-5 46-47 0.0013350865791051394 5.038062562660903E-5 0.0 0.10466574973928026 5.038062562660903E-5 48-49 0.001360276891918444 5.038062562660903E-5 0.0 0.13061177193698392 5.038062562660903E-5 50-51 0.001360276891918444 5.038062562660903E-5 0.0 0.162880562650827 5.038062562660903E-5 52-53 0.001360276891918444 5.038062562660903E-5 0.0 0.19532568555436322 5.038062562660903E-5 54-55 0.001360276891918444 5.038062562660903E-5 0.0 0.2298112237957771 5.038062562660903E-5 56-57 0.0014610381431716618 5.038062562660903E-5 0.0 0.2705691499277038 5.038062562660903E-5 58-59 0.0014610381431716618 5.038062562660903E-5 0.0 0.3177002252013965 5.038062562660903E-5 60-61 0.0014610381431716618 5.038062562660903E-5 0.0 0.37389981308787895 5.038062562660903E-5 62-63 0.0014862284559849664 5.038062562660903E-5 0.0 0.43307185788633124 5.038062562660903E-5 64-65 0.001511418768798271 5.038062562660903E-5 0.0 0.4928232798794896 5.038062562660903E-5 66-67 0.001511418768798271 5.038062562660903E-5 0.0 0.5588974703887872 5.038062562660903E-5 68-69 0.001511418768798271 5.038062562660903E-5 0.0 0.6409171289089068 5.038062562660903E-5 70-71 0.001511418768798271 5.038062562660903E-5 0.0 0.733415957559361 5.038062562660903E-5 72-73 0.0015869897072381845 5.038062562660903E-5 0.0 0.8300711878240103 5.038062562660903E-5 74-75 0.001662560645678098 5.038062562660903E-5 0.0 0.9273309855961791 5.038062562660903E-5 76-77 0.001662560645678098 5.038062562660903E-5 0.0 1.0373622719646933 5.038062562660903E-5 78-79 0.0016877509584914026 5.038062562660903E-5 0.0 1.1648000644872007 5.038062562660903E-5 80-81 0.0019396540866244477 5.038062562660903E-5 0.0 1.3106771659890473 5.038062562660903E-5 82-83 0.0019648443994377523 5.038062562660903E-5 0.0 1.46582430260619 5.038062562660903E-5 84-85 0.0019648443994377523 5.038062562660903E-5 0.0 1.6260346920988065 5.038062562660903E-5 86-87 0.0019648443994377523 5.038062562660903E-5 0.0 1.790376292892805 5.038062562660903E-5 88-89 0.0019648443994377523 5.038062562660903E-5 0.0 1.9784975489825634 5.038062562660903E-5 90-91 0.002191557214757493 5.038062562660903E-5 0.0 2.1917335469471864 5.038062562660903E-5 92-93 0.0022671281531974063 5.038062562660903E-5 0.0 2.4252981273521454 5.038062562660903E-5 94-95 0.0022671281531974063 5.038062562660903E-5 0.0 2.667880839744268 5.038062562660903E-5 96-97 0.0022671281531974063 5.038062562660903E-5 0.0 2.9151237600068516 5.038062562660903E-5 98-99 0.00234269909163732 5.038062562660903E-5 0.0 3.1815365083203604 5.038062562660903E-5 100-101 0.002493840968517147 5.038062562660903E-5 0.0 3.497045176306999 5.038062562660903E-5 102-103 0.0025946022197703648 5.038062562660903E-5 0.0 3.8390540533732347 5.038062562660903E-5 104-105 0.003022837537596542 5.038062562660903E-5 0.0 4.180382791993511 5.038062562660903E-5 106-107 0.003022837537596542 5.038062562660903E-5 0.0 4.51974668621435 5.038062562660903E-5 108-109 0.0030480278504098467 5.038062562660903E-5 0.0 4.885157363884145 5.038062562660903E-5 110-111 0.003173979414476369 5.038062562660903E-5 0.0 5.296338839935714 5.038062562660903E-5 112-113 0.003173979414476369 5.038062562660903E-5 0.0 5.757850560988267 5.038062562660903E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2825 0.0 55.599762 1 GTATCAA 5050 0.0 52.30932 1 ATCAACG 6030 0.0 43.51087 3 TCAACGC 6080 0.0 43.34876 4 CAACGCA 6130 0.0 42.898125 5 AACGCAG 6455 0.0 41.28607 6 TATCAAC 6585 0.0 39.66297 2 GTACATG 9295 0.0 37.63703 1 TACATGG 9475 0.0 35.66528 2 ACGCAGA 7550 0.0 34.51032 7 ACATGGG 9835 0.0 33.573387 3 CGCAGAG 7690 0.0 33.26822 8 GCAGAGT 8725 0.0 28.231457 9 CATGGGA 5830 0.0 27.961374 4 CATGGGG 4125 0.0 27.40351 4 GAGTACT 3970 0.0 23.986074 12-13 CAGAGTA 8470 0.0 23.360264 10-11 GAGTACA 7070 0.0 23.310297 1 AGTACAT 6915 0.0 22.541645 2 AGAGTAC 8180 0.0 22.115145 10-11 >>END_MODULE