FastQCFastQC Report
Thu 26 May 2016
SRR1364536_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364536_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1984890
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53420.26913330209734543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46310.23331267727682645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30060.15144416063358673No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38600.060.3903431
GTATCAA67550.050.3951531
ATCAACG84400.039.6562543
TCAACGC85150.039.2370224
CAACGCA86450.038.4723475
TATCAAC88700.037.666662
AACGCAG89750.037.1903536
GTACATG85950.033.2252041
TACATGG89200.031.513382
ACGCAGA105500.031.469057
CGCAGAG106350.031.0496968
ACATGGG90250.030.4868553
GCAGAGT118550.026.9001189
CATGGGG36550.025.255864
GTGGTAT22000.025.2021921
TGGTATC21100.024.555832
GAGTACT72000.024.5422812-13
CATGGGA58950.024.2462884
AGAGTAC113650.023.76656710-11
AGTACTT74900.023.3537412-13
Produced by FastQC (version 0.11.5)