FastQCFastQC Report
Thu 26 May 2016
SRR1364535_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364535_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924610
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42040.2184338645232021No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30440.15816191332269916No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA21760.11306186707956419No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35250.057.5538371
GTATCAA61900.050.5561641
TCAACGC73750.041.303514
ATCAACG74400.040.8626943
CAACGCA76200.040.1316645
AACGCAG77750.039.2499966
GTACATG104200.038.4261051
TATCAAC80500.038.3575172
TACATGG107550.036.4547422
ACATGGG109250.034.7983323
ACGCAGA92600.033.0840647
CGCAGAG93350.031.6728578
TACCTGG17450.031.3668862
CATGGGG51500.029.1120194
GCAGAGT102800.027.4324289
GAGTACT46450.027.41913812-13
CATGGGA63400.027.1198714
GTACTTT49100.025.51504514-15
GTACCTG21500.025.4582421
CAGAGTA96350.024.48810410-11
Produced by FastQC (version 0.11.5)