##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364535_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1924610 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.51304316199126 31.0 26.0 31.0 2.0 34.0 2 25.56175640779171 31.0 25.0 31.0 2.0 34.0 3 25.721646463439345 31.0 26.0 31.0 2.0 34.0 4 28.757035451338194 35.0 27.0 37.0 2.0 37.0 5 28.817504845137456 35.0 28.0 37.0 2.0 37.0 6 28.787515392728917 35.0 28.0 37.0 2.0 37.0 7 28.488221509812377 35.0 27.0 37.0 2.0 37.0 8 28.388881383760864 35.0 27.0 37.0 2.0 37.0 9 29.208182436961255 35.0 27.0 39.0 2.0 39.0 10-11 29.102672229698484 35.0 27.0 39.0 2.0 39.0 12-13 28.96741028052437 35.0 26.5 39.0 2.0 39.0 14-15 29.440994279360496 36.0 25.0 40.0 2.0 41.0 16-17 29.51169613584051 36.0 26.0 40.0 2.0 41.0 18-19 29.303456804235665 36.0 25.0 40.0 2.0 41.0 20-21 28.98775102488296 36.0 23.0 40.0 2.0 41.0 22-23 28.861323592831795 36.0 22.5 40.0 2.0 41.0 24-25 28.6571029974904 36.0 22.0 40.0 2.0 41.0 26-27 28.49378705296138 35.0 20.5 40.0 2.0 41.0 28-29 28.513300356955433 35.5 22.0 40.0 2.0 41.0 30-31 28.4228841167821 35.0 22.0 40.0 2.0 41.0 32-33 28.352431401686573 35.0 22.5 40.0 2.0 41.0 34-35 27.875960324429364 35.0 18.0 39.5 2.0 41.0 36-37 27.729711993598706 35.0 17.0 39.0 2.0 41.0 38-39 27.51159014034012 34.0 16.0 39.0 2.0 41.0 40-41 27.683904791100534 35.0 18.0 39.0 2.0 41.0 42-43 27.541970321259893 35.0 17.0 39.0 2.0 41.0 44-45 27.367093073401882 34.0 15.5 39.0 2.0 41.0 46-47 27.28401832059482 34.0 15.0 39.0 2.0 41.0 48-49 27.09793204857088 34.0 14.5 39.0 2.0 40.5 50-51 26.919615922186832 34.0 12.5 39.0 2.0 40.0 52-53 26.829812533448333 34.0 10.5 39.0 2.0 40.0 54-55 26.820218381905946 34.0 9.0 39.0 2.0 41.0 56-57 26.6822706938029 34.0 8.0 39.0 2.0 40.5 58-59 26.468622993749385 33.5 7.5 39.0 2.0 40.0 60-61 26.256475857446446 33.0 7.0 38.0 2.0 40.0 62-63 25.969261564680636 33.0 3.5 38.0 2.0 40.0 64-65 25.69102987098685 33.0 2.0 37.5 2.0 40.0 66-67 25.45577701456399 32.5 2.0 37.0 2.0 40.0 68-69 25.413985690607447 33.0 2.0 37.0 2.0 40.0 70-71 25.19792295581962 32.5 2.0 36.5 2.0 39.0 72-73 24.941093780038557 32.0 2.0 36.0 2.0 39.0 74-75 24.675485423020767 32.0 2.0 35.5 2.0 39.0 76-77 24.40901455359787 32.0 2.0 35.0 2.0 37.5 78-79 24.14193005336146 32.0 2.0 35.0 2.0 37.0 80-81 23.875488020949696 32.0 2.0 35.0 2.0 37.0 82-83 23.658993770166425 31.5 2.0 35.0 2.0 36.0 84-85 23.44378809213295 31.0 2.0 35.0 2.0 36.0 86-87 23.27242220501816 31.0 2.0 35.0 2.0 35.5 88-89 23.09108416770151 31.0 2.0 35.0 2.0 35.0 90-91 22.966414754157984 31.0 2.0 35.0 2.0 35.0 92-93 22.85095266053902 31.0 2.0 35.0 2.0 35.0 94-95 22.727756532492297 31.0 2.0 35.0 2.0 35.0 96-97 22.613744083216858 31.0 2.0 35.0 2.0 35.0 98-99 22.515893349821525 31.0 2.0 35.0 2.0 35.0 100-101 22.419642940647712 31.0 2.0 35.0 2.0 35.0 102-103 21.45418448412925 29.0 2.0 33.5 2.0 35.0 104-105 22.17719460046451 30.5 2.0 34.0 2.0 35.0 106-107 22.301336894227923 31.0 2.0 35.0 2.0 35.0 108-109 22.240588742654356 31.0 2.0 35.0 2.0 35.0 110-111 22.149439107143785 31.0 2.0 35.0 2.0 35.0 112-113 22.04159959680143 31.0 2.0 35.0 2.0 35.0 114-115 21.905931331542494 31.0 2.0 35.0 2.0 35.0 116-117 21.78226991442422 31.0 2.0 35.0 2.0 35.0 118-119 21.61432394095427 30.0 2.0 35.0 2.0 35.0 120-121 21.422706158650325 30.0 2.0 34.5 2.0 35.0 122-123 21.166484638446228 30.0 2.0 34.0 2.0 35.0 124-125 19.88717532383184 27.5 2.0 33.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.869334652354258 1101 2 0.7641259812134038 1101 3 0.7928385959351303 1101 4 0.8359108493040743 1101 5 0.7598984418201589 1101 6 0.7771278653403186 1101 7 0.7633083320648275 1101 8 0.7359958576320302 1101 9 0.9053069582014572 1101 10-11 1.1776592150004213 1101 12-13 0.9514016779839736 1101 14-15 1.2140297409242962 1101 16-17 1.0525004721164386 1101 18-19 1.3936689368167272 1101 20-21 1.7184111135955646 1101 22-23 1.5717076405385662 1101 24-25 1.8016366493931955 1101 26-27 1.834697449913108 1101 28-29 1.6073699714288487 1101 30-31 1.615029674647598 1101 32-33 1.5662472907440836 1101 34-35 1.8671954425713686 1101 36-37 1.9043788488436562 1101 38-39 1.7517200424389046 1101 40-41 1.5210508046015327 1101 42-43 1.7039622762084896 1101 44-45 1.5708152340034367 1101 46-47 1.4872556397074348 1101 48-49 1.4857057980427442 1101 50-51 1.4887782113769816 1101 52-53 1.2239884198866022 1101 54-55 1.193014619388535 1101 56-57 1.2124201040524802 1101 58-59 1.2463054058336631 1101 60-61 1.1974039707380228 1101 62-63 1.2219096016808066 1101 64-65 1.3845864737070492 1101 66-67 1.1986720976962602 1101 68-69 1.0634924032810211 1101 70-71 0.9741948876061208 1101 72-73 0.9869728959413173 1101 74-75 0.9693061545238955 1101 76-77 1.1697103385521963 1101 78-79 1.1201174106140321 1101 80-81 1.2165446751585733 1101 82-83 1.0595070408026324 1101 84-85 1.1368459863652305 1101 86-87 1.1552976267033017 1101 88-89 1.0887842428368906 1101 90-91 1.097070287830821 1101 92-93 0.9843001495513128 1101 94-95 1.1066794063841208 1101 96-97 1.1053529037164687 1101 98-99 1.2075543541528688 1101 100-101 1.3023397758411264 1101 102-103 1.202428098219432 1101 104-105 1.3416762358128231 1101 106-107 1.2763292627615392 1101 108-109 1.304985310182058 1101 110-111 1.111509592316473 1101 112-113 1.1818969299132966 1101 114-115 1.3335508609242837 1101 116-117 1.4316719022350703 1101 118-119 1.3199536348306964 1101 120-121 1.498702493083158 1101 122-123 1.635005883906139 1101 124-125 1.7301128766526936 1102 1 -0.6894532579782897 1102 2 -0.41234367989089193 1102 3 -0.21092002906031837 1102 4 -0.12091316327949642 1102 5 -0.2268353701168806 1102 6 -1.0587229880018327 1102 7 -1.4963121864624362 1102 8 -0.633156024637227 1102 9 -0.4373016739170872 1102 10-11 -0.36722051217640583 1102 12-13 -0.1689309523253293 1102 14-15 -0.05504684177779495 1102 16-17 -0.11272489754542292 1102 18-19 0.16540043099815094 1102 20-21 0.418378381559144 1102 22-23 0.3375065600270446 1102 24-25 0.4271362741782525 1102 26-27 -0.012148569741754045 1102 28-29 0.5633859462265285 1102 30-31 0.32506470122542197 1102 32-33 0.23060939723572815 1102 34-35 0.5859150884642474 1102 36-37 0.7185278366674375 1102 38-39 0.6529363082205961 1102 40-41 0.5131632268675261 1102 42-43 0.33313151781979045 1102 44-45 0.46305951298350934 1102 46-47 0.1645657163118841 1102 48-49 -0.20855511159752993 1102 50-51 0.14318979101602025 1102 52-53 -0.017632777473039596 1102 54-55 -0.15587382313588805 1102 56-57 -0.00892635190735902 1102 58-59 0.1307665710257382 1102 60-61 0.05275201161751397 1102 62-63 -0.08845235428501041 1102 64-65 -0.11935334430820532 1102 66-67 0.08554827383200347 1102 68-69 -0.06470043643023615 1102 70-71 -0.13982250008169572 1102 72-73 0.08052714058635146 1102 74-75 -0.3653320617841551 1102 76-77 -0.10914223679563406 1102 78-79 -0.16796360746836925 1102 80-81 -0.563474414253033 1102 82-83 -0.31818980898016846 1102 84-85 -0.6798360383341979 1102 86-87 -0.1903754033449303 1102 88-89 0.01225065712843687 1102 90-91 -0.48304060767322454 1102 92-93 0.030571507541068854 1102 94-95 -0.10688216417694107 1102 96-97 0.10968675240527048 1102 98-99 0.09665768939953878 1102 100-101 0.06744569006437473 1102 102-103 -0.1529096184455092 1102 104-105 0.20476071769975235 1102 106-107 0.12487046957302894 1102 108-109 -0.2953809611832625 1102 110-111 -0.061952912213349975 1102 112-113 -0.366812042330082 1102 114-115 -0.3560753316446785 1102 116-117 -0.3406114792494215 1102 118-119 -0.03311758074599069 1102 120-121 0.19344833535701866 1102 122-123 0.11967179843239606 1102 124-125 0.30321521881989 1103 1 0.7392639471718283 1103 2 0.7875351087041551 1103 3 0.8494454911577094 1103 4 0.8866340502000583 1103 5 0.9331160419110454 1103 6 1.0024817543851512 1103 7 1.0302127492817341 1103 8 1.0784002258998306 1103 9 1.4868729965678753 1103 10-11 1.5565117723815831 1103 12-13 1.4025430896478426 1103 14-15 1.9383668584348612 1103 16-17 1.7017676703463813 1103 18-19 1.559111718382436 1103 20-21 2.0134397739169927 1103 22-23 1.9884651100599235 1103 24-25 2.075974363268017 1103 26-27 2.038743539304683 1103 28-29 2.0466032879025917 1103 30-31 1.913657422779707 1103 32-33 1.7966105214511963 1103 34-35 2.1543496738230026 1103 36-37 2.0418209114656847 1103 38-39 2.094120277489086 1103 40-41 1.9366759496331873 1103 42-43 1.8556155809698716 1103 44-45 1.420863515889824 1103 46-47 1.4730652998400338 1103 48-49 1.417844033151738 1103 50-51 1.4220993143225584 1103 52-53 1.335135129154537 1103 54-55 1.2121145722816635 1103 56-57 1.0586951715744917 1103 58-59 1.009155313704241 1103 60-61 0.8774141006639482 1103 62-63 0.8332071733918376 1103 64-65 1.145317162812443 1103 66-67 1.1486029489654968 1103 68-69 1.0885841636644606 1103 70-71 1.2357397615364896 1103 72-73 1.1447440713303934 1103 74-75 1.1006671912010084 1103 76-77 1.1108285793980919 1103 78-79 1.1136997636170385 1103 80-81 0.9642583267866662 1103 82-83 1.1435167555173251 1103 84-85 1.0691044390609683 1103 86-87 1.0095340389570389 1103 88-89 0.9671272925775654 1103 90-91 1.0448562353396653 1103 92-93 1.090395417383224 1103 94-95 0.9651316415632571 1103 96-97 1.1736324778627925 1103 98-99 1.0876097369071225 1103 100-101 1.087429937259806 1103 102-103 0.9405500869489778 1103 104-105 0.9849855189369308 1103 106-107 0.9905337382331929 1103 108-109 1.2629627685579017 1103 110-111 1.3121339089332267 1103 112-113 1.2154564132327508 1103 114-115 1.256832673503176 1103 116-117 1.3048790157623955 1103 118-119 1.3635698861227716 1103 120-121 1.3524695489532803 1103 122-123 1.3237532225568351 1103 124-125 1.361844249628465 1104 1 0.6238950747831531 1104 2 0.7136783009312673 1104 3 0.6081341672323894 1104 4 0.5429345954208245 1104 5 0.504614782121525 1104 6 0.5210867103735666 1104 7 0.6015902101160755 1104 8 0.7450879998425357 1104 9 0.8846923234160258 1104 10-11 0.6908689718635372 1104 12-13 0.7203060169214979 1104 14-15 0.6953274200143476 1104 16-17 0.8541424371459669 1104 18-19 1.0777630870551818 1104 20-21 1.3554177029363714 1104 22-23 0.9555948423753513 1104 24-25 1.6271900607375471 1104 26-27 1.7375557127687102 1104 28-29 1.6410231888220075 1104 30-31 1.4709334156904568 1104 32-33 1.370269492241757 1104 34-35 1.5984170249251655 1104 36-37 1.6917974369095106 1104 38-39 1.7627705616443308 1104 40-41 1.606102130258762 1104 42-43 1.6002867517659851 1104 44-45 1.3693319089684088 1104 46-47 1.379790497799462 1104 48-49 1.1679243807384267 1104 50-51 1.0889638179715533 1104 52-53 1.3371648677904844 1104 54-55 1.0360486373494524 1104 56-57 1.0431948510004467 1104 58-59 1.0563956131616834 1104 60-61 1.0801806231714188 1104 62-63 1.0450454515418883 1104 64-65 1.1718389799226792 1104 66-67 1.241330944280378 1104 68-69 1.1984128425518854 1104 70-71 1.1200921798122643 1104 72-73 0.972958365457604 1104 74-75 1.080780755342989 1104 76-77 1.055034177327574 1104 78-79 1.0543308851643935 1104 80-81 1.0846141721377371 1104 82-83 1.0378149572473738 1104 84-85 0.9771428944188472 1104 86-87 1.0351003145210385 1104 88-89 1.0748895574792456 1104 90-91 1.0699963421728036 1104 92-93 1.0239983990833323 1104 94-95 1.0565739588762817 1104 96-97 0.9156996988228521 1104 98-99 0.9779323657029053 1104 100-101 0.9875827842581728 1104 102-103 0.9699202784840963 1104 104-105 0.9316205526770851 1104 106-107 0.8605044632576302 1104 108-109 1.0057423309305982 1104 110-111 1.0557430105772383 1104 112-113 1.120004821312662 1104 114-115 1.006441372592949 1104 116-117 1.0266790535922325 1104 118-119 1.0199519450780734 1104 120-121 0.9984394819300988 1104 122-123 0.9732702857487574 1104 124-125 1.257597628950002 1105 1 0.19603164664623307 1105 2 0.3890089946542865 1105 3 0.5314145357004421 1105 4 0.5770699641962089 1105 5 0.6691359884413934 1105 6 0.4622918423506306 1105 7 0.8678662531316377 1105 8 0.5124207157473961 1105 9 0.4554762849172178 1105 10-11 0.7086633778510745 1105 12-13 0.7462024450218152 1105 14-15 0.93409578143239 1105 16-17 0.8430627384565881 1105 18-19 0.8648687080715085 1105 20-21 1.2809887379440141 1105 22-23 1.3523452969864174 1105 24-25 1.4251566215297302 1105 26-27 1.233504615382838 1105 28-29 0.9855481038565266 1105 30-31 0.9067845657027895 1105 32-33 1.0688447449626928 1105 34-35 1.6477791881624775 1105 36-37 1.7390414199171076 1105 38-39 1.896581953130589 1105 40-41 1.3753957340727254 1105 42-43 0.9328246452298785 1105 44-45 1.11398483336243 1105 46-47 1.2201959424964457 1105 48-49 1.140327089136079 1105 50-51 1.0960588929780641 1105 52-53 0.8986188565925133 1105 54-55 0.7556646299900329 1105 56-57 0.8543456506531975 1105 58-59 0.8530465126113143 1105 60-61 0.8942407264491052 1105 62-63 0.8714681096474308 1105 64-65 0.9221830892475751 1105 66-67 0.7527533710229051 1105 68-69 0.6388498147765844 1105 70-71 0.9057809613302972 1105 72-73 1.0342837233278495 1105 74-75 0.9404880914844505 1105 76-77 1.0861088205623481 1105 78-79 1.0923629942075088 1105 80-81 1.0089279045342927 1105 82-83 0.8964509444871887 1105 84-85 0.945016412745904 1105 86-87 0.9540424537380403 1105 88-89 1.1369616653897197 1105 90-91 1.02163611893166 1105 92-93 0.9307185278731112 1105 94-95 0.9722124413835154 1105 96-97 1.082941665173692 1105 98-99 0.8857279872095845 1105 100-101 0.8767876966130288 1105 102-103 0.784096926565752 1105 104-105 0.9106046756489476 1105 106-107 0.8237700590243087 1105 108-109 0.7814636828365131 1105 110-111 0.7495174806695957 1105 112-113 0.8984193078145317 1105 114-115 0.9095482671418331 1105 116-117 0.9468878190046333 1105 118-119 0.8552076633704608 1105 120-121 0.8171608593895385 1105 122-123 0.8414803854696693 1105 124-125 0.8903223048863858 1106 1 0.30539557370071435 1106 2 0.6991961132978766 1106 3 0.4929420056346281 1106 4 0.7669468396167716 1106 5 0.5023579896357155 1106 6 0.5213217203902154 1106 7 0.3257720096869967 1106 8 0.6403281940060701 1106 9 0.8510935506209236 1106 10-11 0.9346006454342728 1106 12-13 0.8743625683392544 1106 14-15 1.1469452986015582 1106 16-17 0.9971655294042847 1106 18-19 1.026364829680567 1106 20-21 1.3713821944895912 1106 22-23 1.399336751937927 1106 24-25 1.481558009131433 1106 26-27 1.8391951078285587 1106 28-29 1.5280343766914513 1106 30-31 1.211256225449329 1106 32-33 1.2134960509500452 1106 34-35 1.3892389611430964 1106 36-37 1.3387413666530072 1106 38-39 1.4570994740040462 1106 40-41 1.3456798712241067 1106 42-43 1.2737925580801281 1106 44-45 1.0667319670419992 1106 46-47 0.8632175914253608 1106 48-49 1.05648802736971 1106 50-51 0.9287742014452292 1106 52-53 1.0117691283305987 1106 54-55 0.8303295728494717 1106 56-57 0.7893450387647469 1106 58-59 0.7145514355957729 1106 60-61 0.8396783353924242 1106 62-63 0.7156869209568129 1106 64-65 0.8286439299647732 1106 66-67 0.7737087094778659 1106 68-69 0.7322488884230545 1106 70-71 0.903723157683725 1106 72-73 0.7908774788396649 1106 74-75 0.8649701866466017 1106 76-77 0.7115980245007343 1106 78-79 0.7481666246521321 1106 80-81 0.7389048631646702 1106 82-83 0.7116518665627538 1106 84-85 0.7466818012845522 1106 86-87 0.8581102671082022 1106 88-89 0.7629179383333451 1106 90-91 0.7552491676895272 1106 92-93 0.797185357733408 1106 94-95 0.8344393576726148 1106 96-97 0.8577253389379145 1106 98-99 0.7468959332315528 1106 100-101 0.8461685531656897 1106 102-103 0.8742678941595088 1106 104-105 0.8976421081439767 1106 106-107 0.8451872066984158 1106 108-109 0.8081749805414589 1106 110-111 0.7524589283801362 1106 112-113 0.6761595073427671 1106 114-115 0.8252768509584492 1106 116-117 0.9240012624217862 1106 118-119 0.9963969781500772 1106 120-121 0.9150581021044744 1106 122-123 0.9931563111131254 1106 124-125 1.052197481746692 1107 1 0.5813385118503547 1107 2 0.7283238755558799 1107 3 0.49395271631190596 1107 4 0.6536364904897205 1107 5 0.6542065302407813 1107 6 0.6152187855562126 1107 7 0.7473952045334649 1107 8 0.5378421777675975 1107 9 0.5854410310014018 1107 10-11 0.8798517273973872 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2216 14-15 -2.2105059818674704 2216 16-17 -2.181169014234875 2216 18-19 -5.479453325727018 2216 20-21 -4.746035493112942 2216 22-23 -3.067310608111491 2216 24-25 -4.652813187079161 2216 26-27 -6.172785894632739 2216 28-29 -5.16293839299226 2216 30-31 -3.454054931066686 2216 32-33 -2.959030373700781 2216 34-35 -5.689756777479623 2216 36-37 -4.843793256565245 2216 38-39 -5.50685720837879 2216 40-41 -4.511390349068826 2216 42-43 -5.928073989271855 2216 44-45 -5.5692120871512145 2216 46-47 -4.187849004748948 2216 48-49 -2.774630764659822 2216 50-51 -2.524117220794153 2216 52-53 -2.687727744893131 2216 54-55 -2.435781335195024 2216 56-57 -2.1665254732965984 2216 58-59 -2.0091244126678163 2216 60-61 -2.21008417379009 2216 62-63 -2.2873275121694974 2216 64-65 -2.3797264868585124 2216 66-67 -2.2497414920221317 2216 68-69 -2.2321216613558015 2216 70-71 -2.179456526704346 2216 72-73 -2.1542269231230264 2216 74-75 -2.0687331668016604 2216 76-77 -2.1674829805182334 2216 78-79 -2.3786217330583668 2216 80-81 -2.363114205384395 2216 82-83 -2.1437092486582543 2216 84-85 -2.175769916793943 2216 86-87 -2.175346730018898 2216 88-89 -2.349732665961618 2216 90-91 -2.3897860477314197 2216 92-93 -2.377653598207033 2216 94-95 -2.367025085398385 2216 96-97 -2.321000247337359 2216 98-99 -2.4165624787069078 2216 100-101 -2.395123261335815 2216 102-103 -2.173555265859971 2216 104-105 -2.4552992587709994 2216 106-107 -2.4723987150328384 2216 108-109 -2.7120609227950894 2216 110-111 -2.656653255368788 2216 112-113 -2.6434170787918916 2216 114-115 -2.6785298657370653 2216 116-117 -2.727256532935197 2216 118-119 -2.834190477210793 2216 120-121 -2.8959615732702417 2216 122-123 -2.8273475646174404 2216 124-125 -2.701372434809656 >>END_MODULE >>Per sequence quality scores fail #Quality Count 2 260573.0 3 37179.0 4 22386.0 5 17449.0 6 15574.0 7 15725.0 8 16520.0 9 16726.0 10 17068.0 11 18302.0 12 19226.0 13 20782.0 14 22111.0 15 19406.0 16 14988.0 17 13116.0 18 12045.0 19 11211.0 20 11063.0 21 11801.0 22 14574.0 23 19101.0 24 25814.0 25 31721.0 26 37426.0 27 41164.0 28 46375.0 29 52124.0 30 57911.0 31 65486.0 32 78007.0 33 93476.0 34 117754.0 35 154848.0 36 215538.0 37 224352.0 38 55582.0 39 106.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.76659871302065 18.22292294908777 12.838873989204671 23.17160434868691 2 15.351284448146371 19.821586959469954 40.641655101030224 24.18547349135344 3 19.593404726186808 25.757650551918477 27.757111182357313 26.8918335395374 4 12.22525509660597 16.37144206681642 36.64921248324861 34.754090353329 5 13.824396023551056 38.59478363898084 32.92974697686987 14.651073360598236 6 32.017406214278296 36.23178842356853 17.48373688039073 14.267068481762443 7 29.32494253016906 32.71737729723438 19.73194948474607 18.225730687850483 8 26.66041431770593 35.472485334691186 18.940928291965644 18.926172055637245 9 26.981067959147197 16.875319552231982 18.86958527835857 37.27402721026225 10-11 25.275302528824025 27.42846602688337 26.15542889208723 21.140802552205383 12-13 26.07671699521032 24.677743518758113 26.715774148192256 22.529765337839315 14-15 23.08598351001178 25.721544168831784 24.712688030220473 26.479784290935964 16-17 22.68287390448471 29.327936695620537 25.042287377454965 22.94690202243979 18-19 22.58515239369282 27.55772846583187 27.686332930719086 22.170786209756223 20-21 23.351673547819534 26.59632483822081 27.88689499571178 22.165106618247876 22-23 23.00892979619322 26.585888365596848 27.70229474874362 22.702887089466312 24-25 22.757073050580193 26.534796958739598 28.035368955354645 22.672761035325564 26-27 22.61103466052475 26.822177206454 27.71119243513364 22.85559569788761 28-29 22.452347902850487 26.838401008417073 27.892204122362017 22.817046966370423 30-31 22.486299629582096 26.7303618498137 27.68334750956441 23.099991011039794 32-33 22.499068566496558 26.69140269988407 27.53488913473075 23.27463959888862 34-35 22.892658115108024 26.452594501945885 27.91849803930433 22.736249343641763 36-37 22.87391537960285 26.28221383637341 27.79164064131997 23.052230142703774 38-39 22.639210021770644 26.51168288640296 27.90734226674495 22.941764825081446 40-41 22.90018969415066 26.535431229374005 27.613335758646674 22.951043317828653 42-43 22.860427684525177 26.53306883977484 27.340657570478573 23.26584590522141 44-45 22.835888995883675 26.7494123803649 27.25705442373781 23.157644200013618 46-47 23.132063040420505 26.979755713434304 26.644793075550922 23.243388170594265 48-49 23.14851314827668 27.012064291262188 26.474709995247252 23.36471256521388 50-51 22.993288227924857 27.097435268577403 26.478062944232068 23.43121355926567 52-53 23.372007835353656 27.04888263076675 26.32496973412795 23.254139799751638 54-55 23.2068530157723 27.010928437817245 26.37035501118204 23.41186353522842 56-57 23.103832695040122 27.385103974547597 26.243632547835617 23.26743078257666 58-59 23.25353079035199 26.954810715656123 26.31124992173717 23.480408572254717 60-61 23.14044283366783 27.01773802796776 26.567863707514146 23.27395543085027 62-63 23.150427208282228 26.89154002868844 26.66806124358148 23.289971519447853 64-65 23.089840884707733 26.909990222400758 26.478533843407188 23.521635049484317 66-67 23.034558189319227 27.286654750646893 26.222890189028252 23.45589687100562 68-69 23.024076502666922 27.022760645154754 26.55677974535663 23.3963831068217 70-71 23.03677408158324 26.99484350205397 26.5162410979745 23.452141318388286 72-73 23.114463395591077 27.01080990494403 26.384898091215796 23.4898286082491 74-75 22.88936459854204 27.302336577280588 26.310395352824727 23.49790347135264 76-77 23.18744074788927 27.145540201282458 26.291088716371625 23.375930334456648 78-79 23.144114106203233 27.20411536361194 26.27682839956292 23.374942130621903 80-81 23.02109004825526 27.371467823504318 26.288179565479226 23.319262562761196 82-83 23.208697773467467 27.322884497926697 26.201343718605237 23.2670740100006 84-85 23.08207896664779 27.296439278607092 26.11443357355516 23.507048181189955 86-87 23.159893333776736 27.03885876286303 26.40002016099699 23.401227742363243 88-89 23.263587824282578 27.057605977844997 26.22130425756201 23.45750194031041 90-91 23.12551872233589 27.29735853724069 26.272412572712074 23.30471016771134 92-93 23.1274049721789 27.192972200723446 26.278488471332068 23.40113435576558 94-95 23.168371786999543 27.017333058580768 26.29306727029078 23.521227884128905 96-97 23.344623255681014 26.98714975581808 26.172144682498544 23.496082306002368 98-99 22.982015947259526 27.10971768591194 26.113897268722653 23.794369098105886 100-101 22.783510725916116 27.067433777217502 26.45484348523293 23.694212011633446 102-103 23.063140064740388 26.833150612331846 26.440837364453056 23.662871958474703 104-105 22.999078244278774 26.901160144404933 26.43778824350978 23.661973367806514 106-107 22.811926263483578 27.02789838665974 26.47072042406525 23.689454925791438 108-109 22.87863515205678 27.168803030224304 26.07310052426206 23.879461293456856 110-111 22.783900845787016 27.11771497216562 26.402994287638247 23.695389894409118 112-113 22.94883119177392 27.092527836808493 26.36780958220107 23.590831389216515 114-115 22.72088890736305 27.440208665651745 26.100248881591597 23.73865354539361 116-117 22.656382332004927 27.317846212998997 26.332269914424224 23.693501540571855 118-119 22.816310837000742 27.35541226534207 26.05491502174467 23.77336187591252 120-121 22.597565221005812 27.375104566639475 26.31195411018336 23.715376102171348 122-123 22.691454372574185 27.318885384571416 26.236198502553766 23.753461740300633 124-125 22.810328183446998 27.396659048594117 25.93801686716619 23.854995900792687 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1995.0 1 2014.0 2 1874.0 3 1450.0 4 1006.0 5 771.0 6 746.5 7 917.5 8 1262.0 9 1458.0 10 1356.0 11 1277.0 12 1314.0 13 1393.5 14 1564.0 15 1752.5 16 1887.0 17 2026.5 18 2225.0 19 2477.5 20 2800.0 21 3248.0 22 3803.5 23 4515.5 24 5375.5 25 6535.5 26 8508.5 27 10821.5 28 12873.5 29 15068.5 30 17984.0 31 21746.5 32 25801.0 33 29559.0 34 33647.0 35 37124.0 36 40842.5 37 45126.5 38 47746.0 39 49277.5 40 50709.5 41 53232.0 42 55671.0 43 57914.5 44 60679.0 45 62692.5 46 64235.5 47 66489.0 48 69968.0 49 72379.5 50 72563.0 51 71856.5 52 71206.0 53 71921.0 54 71565.5 55 67897.0 56 63367.5 57 58769.0 58 54635.5 59 49872.0 60 42753.5 61 35118.0 62 28808.0 63 22930.0 64 17640.5 65 13796.0 66 10610.5 67 8240.5 68 6303.0 69 4738.5 70 3552.5 71 2590.0 72 2003.5 73 1537.0 74 1151.5 75 897.5 76 643.5 77 425.0 78 289.0 79 195.5 80 137.5 81 92.0 82 69.0 83 49.0 84 35.5 85 36.5 86 27.5 87 19.5 88 18.5 89 16.5 90 13.5 91 12.5 92 11.0 93 9.5 94 9.0 95 6.5 96 6.0 97 5.0 98 2.5 99 4.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01345727186287092 2 0.004520396340037722 3 0.007326159585578377 4 0.0074300767428206235 5 0.014548402013914507 6 5.195857862112323E-4 7 0.006338946591777034 8 0.0 9 0.002182260302087176 10-11 0.0 12-13 0.002909680402782901 14-15 0.01826344038532482 16-17 0.029746286260593054 18-19 0.005429671465907378 20-21 0.007819766082479047 22-23 0.002182260302087176 24-25 0.010937280799746442 26-27 0.01797766820290864 28-29 4.936064969006708E-4 30-31 0.0014028816227703276 32-33 0.008157496843516349 34-35 0.008599144761795895 36-37 0.010599550038709141 38-39 0.0 40-41 0.02390094616571669 42-43 0.009222647705249375 44-45 0.01667870373738056 46-47 0.0034812247676152567 48-49 0.023926925455027254 50-51 0.004208644868310983 52-53 0.0 54-55 0.016548807290827753 56-57 0.003974831264515928 58-59 2.857721824161778E-4 60-61 0.003766996950031435 62-63 0.025459703524350386 64-65 0.016289014397722134 66-67 0.0015587573586336972 68-69 0.0202378663729275 70-71 0.0024940117738139157 72-73 0.011300990850094304 74-75 0.0 76-77 0.0052218371514228855 78-79 8.832958365590952E-4 80-81 0.0027538046669195316 82-83 0.0013249437548386426 84-85 0.0 86-87 0.0052997750193545705 88-89 0.015821387190132027 90-91 0.021354975813281652 92-93 0.0042346241576215445 94-95 0.02361517398330051 96-97 0.017016434498417863 98-99 8.313372579379719E-4 100-101 0.008261414000758594 102-103 0.0 104-105 0.0011430887296647113 106-107 2.5979289310561622E-5 108-109 0.0 110-111 6.235029434534789E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.023771049719163884 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1924610.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.91614945870724 #Duplication Level Percentage of deduplicated Percentage of total 1 84.55689609604869 55.73665000831524 2 8.682345509976013 11.446135685754292 3 2.7637839011121965 5.4653397809184145 4 1.2835967829854387 3.384390295679359 5 0.7019303490900466 2.313427290011103 6 0.4373686367825554 1.7297793858425978 7 0.2730332369767505 1.2598109759027882 8 0.19749911293031297 1.0414704836701287 9 0.14955777825436314 0.8872445577714115 >10 0.8708641266054324 10.520471359139783 >50 0.05653925607318481 2.5592478329478014 >100 0.02602151280698761 2.945007430136199 >500 2.4158586775389264E-4 0.11550505507055357 >1k 3.221144903385226E-4 0.5955198588403208 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4204 0.2184338645232021 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3044 0.15816191332269916 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2176 0.11306186707956419 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 5.1958578621123244E-5 2 0.0 0.0 0.0 0.0 5.1958578621123244E-5 3 0.0 0.0 0.0 0.0 5.1958578621123244E-5 4 0.0 0.0 0.0 0.0 5.1958578621123244E-5 5 0.0 0.0 0.0 0.0 5.1958578621123244E-5 6 0.0 0.0 0.0 0.0 2.0783431448449297E-4 7 5.1958578621123244E-5 0.0 0.0 0.0 2.0783431448449297E-4 8 5.1958578621123244E-5 0.0 0.0 0.0 2.0783431448449297E-4 9 5.1958578621123244E-5 0.0 0.0 5.1958578621123244E-5 2.0783431448449297E-4 10-11 5.1958578621123244E-5 0.0 0.0 5.1958578621123244E-5 2.0783431448449297E-4 12-13 5.1958578621123244E-5 0.0 0.0 5.1958578621123244E-5 3.637100503478627E-4 14-15 5.1958578621123244E-5 0.0 0.0 5.1958578621123244E-5 3.8968933965842435E-4 16-17 5.1958578621123244E-5 0.0 0.0 5.1958578621123244E-5 4.6762720759010914E-4 18-19 5.1958578621123244E-5 0.0 0.0 1.298964465528081E-4 4.6762720759010914E-4 20-21 5.1958578621123244E-5 0.0 0.0 2.0783431448449297E-4 5.195857862112324E-4 22-23 5.1958578621123244E-5 0.0 0.0 4.1566862896898595E-4 5.195857862112324E-4 24-25 5.1958578621123244E-5 0.0 0.0 5.45565075521794E-4 5.195857862112324E-4 26-27 5.1958578621123244E-5 0.0 0.0 7.793786793168486E-4 5.195857862112324E-4 28-29 5.1958578621123244E-5 0.0 0.0 0.0014288609120808892 5.195857862112324E-4 30-31 5.1958578621123244E-5 0.0 0.0 0.002961638981404025 5.715443648323557E-4 32-33 5.1958578621123244E-5 5.1958578621123244E-5 0.0 0.006157091566603104 6.494822327640405E-4 34-35 5.1958578621123244E-5 5.1958578621123244E-5 0.0 0.010573570749398579 7.274201006957254E-4 36-37 5.1958578621123244E-5 5.1958578621123244E-5 0.0 0.019120756932573355 7.793786793168486E-4 38-39 5.1958578621123244E-5 5.1958578621123244E-5 0.0 0.03190256727336967 7.793786793168486E-4 40-41 5.1958578621123244E-5 5.1958578621123244E-5 0.0 0.04920477395420371 7.793786793168486E-4 42-43 5.1958578621123244E-5 5.1958578621123244E-5 0.0 0.06663687708159055 8.053579686274103E-4 44-45 5.1958578621123244E-5 5.1958578621123244E-5 0.0 0.08788793573762996 8.313372579379719E-4 46-47 5.1958578621123244E-5 5.1958578621123244E-5 0.0 0.11428289367716057 9.612337044907799E-4 48-49 5.1958578621123244E-5 5.1958578621123244E-5 0.0 0.1449904136422444 9.872129938013415E-4 50-51 5.1958578621123244E-5 5.1958578621123244E-5 0.0 0.18086781218012998 0.0010391715724224649 52-53 5.1958578621123244E-5 5.1958578621123244E-5 0.0 0.21609572848525155 0.0011430887296647113 54-55 5.1958578621123244E-5 5.1958578621123244E-5 0.0 0.2527265264131434 0.001221026597596396 56-57 5.1958578621123244E-5 5.1958578621123244E-5 0.0 0.2973849247379988 0.0012729851762175194 58-59 5.1958578621123244E-5 5.1958578621123244E-5 0.0 0.3532663760450169 0.001298964465528081 60-61 5.1958578621123244E-5 1.0391715724224649E-4 0.0 0.41348636866689875 0.001298964465528081 62-63 5.1958578621123244E-5 1.0391715724224649E-4 0.0 0.478720364125719 0.0013509230441492042 64-65 5.1958578621123244E-5 1.0391715724224649E-4 0.0 0.5400574661879549 0.0013509230441492042 66-67 5.1958578621123244E-5 1.0391715724224649E-4 0.0 0.6094741272257755 0.0013509230441492042 68-69 5.1958578621123244E-5 1.0391715724224649E-4 0.0 0.6938029003278586 0.0013509230441492042 70-71 5.1958578621123244E-5 1.0391715724224649E-4 0.0 0.7971744924945834 0.0013769023334597658 72-73 5.1958578621123244E-5 1.0391715724224649E-4 0.0 0.9023906142023579 0.0015587573586336972 74-75 5.1958578621123244E-5 1.0391715724224649E-4 0.0 1.0052166412935608 0.0016107159372548204 76-77 5.1958578621123244E-5 1.0391715724224649E-4 0.0 1.1191098456310629 0.0016107159372548204 78-79 5.1958578621123244E-5 1.0391715724224649E-4 0.0 1.2551893630397846 0.001636695226565382 80-81 5.1958578621123244E-5 1.298964465528081E-4 0.0 1.4139488000166267 0.0017406123838076286 82-83 5.1958578621123244E-5 1.5587573586336973E-4 0.0 1.580398106629395 0.0018185502517393134 84-85 5.1958578621123244E-5 1.5587573586336973E-4 0.0 1.7490556528335612 0.0018185502517393134 86-87 7.793786793168487E-5 1.5587573586336973E-4 0.0 1.9243898763905416 0.0018964881196709981 88-89 1.0391715724224649E-4 1.5587573586336973E-4 0.0 2.125963182151189 0.001974425987602683 90-91 1.0391715724224649E-4 1.5587573586336973E-4 0.0 2.3597508066569333 0.001974425987602683 92-93 1.0391715724224649E-4 1.5587573586336973E-4 0.0 2.6182707145863318 0.0020263845662238066 94-95 1.0391715724224649E-4 1.5587573586336973E-4 0.0 2.8730496048550096 0.002182260302087176 96-97 1.0391715724224649E-4 1.5587573586336973E-4 0.0 3.1225806786829535 0.0022601981700188607 98-99 1.0391715724224649E-4 1.5587573586336973E-4 0.0 3.394427962028671 0.0022861774593294225 100-101 1.0391715724224649E-4 1.5587573586336973E-4 0.0 3.7252222528200516 0.0022861774593294225 102-103 1.0391715724224649E-4 1.5587573586336973E-4 0.0 4.080099344802323 0.0023900946165716693 104-105 1.0391715724224649E-4 1.5587573586336973E-4 0.0 4.439678688149806 0.0024680324845033543 106-107 1.0391715724224649E-4 1.5587573586336973E-4 0.0 4.793802380742072 0.0024940117738139157 108-109 1.0391715724224649E-4 1.5587573586336973E-4 0.0 5.155849756574059 0.002545970352435039 110-111 1.0391715724224649E-4 1.5587573586336973E-4 0.0 5.5849496781166055 0.0026498875096772853 112-113 1.0391715724224649E-4 1.5587573586336973E-4 0.0 6.047458965712535 0.0026498875096772853 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3525 0.0 57.553837 1 GTATCAA 6190 0.0 50.556164 1 TCAACGC 7375 0.0 41.30351 4 ATCAACG 7440 0.0 40.862694 3 CAACGCA 7620 0.0 40.131664 5 AACGCAG 7775 0.0 39.249996 6 GTACATG 10420 0.0 38.426105 1 TATCAAC 8050 0.0 38.357517 2 TACATGG 10755 0.0 36.454742 2 ACATGGG 10925 0.0 34.798332 3 ACGCAGA 9260 0.0 33.084064 7 CGCAGAG 9335 0.0 31.672857 8 TACCTGG 1745 0.0 31.366886 2 CATGGGG 5150 0.0 29.112019 4 GCAGAGT 10280 0.0 27.432428 9 GAGTACT 4645 0.0 27.419138 12-13 CATGGGA 6340 0.0 27.119871 4 GTACTTT 4910 0.0 25.515045 14-15 GTACCTG 2150 0.0 25.458242 1 CAGAGTA 9635 0.0 24.488104 10-11 >>END_MODULE