FastQCFastQC Report
Thu 26 May 2016
SRR1364535_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364535_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1924610
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72570.37706340505349134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62570.32510482643236815No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39740.20648339144034375No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45800.061.1647341
GTATCAA82900.052.197631
TATAACG1056.330083E-945.381162
ATCAACG108700.039.726783
TCAACGC109050.039.4900364
TATCAAC110100.038.897032
CAACGCA112200.038.2874455
AACGCAG113350.037.8465046
GTACATG105700.032.1980671
ACGCAGA132950.031.9985087
CGCAGAG134300.031.5882478
TACATGG109100.030.7367922
ACATGGG108550.030.014593
GCAGAGT147250.028.2841749
GAGTACT82400.026.82384912-13
CATGGGA65700.025.3844384
AGTACTT87450.025.20680812-13
CATGGGG46850.024.918474
GTACTTT89650.024.82051514-15
AGAGTAC134400.024.79009610-11
Produced by FastQC (version 0.11.5)