FastQCFastQC Report
Thu 26 May 2016
SRR1364534_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364534_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3009052
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53220.17686633531092186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37490.1245907348892608No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA104000.048.516161
GGTATCA61850.045.9845621
ATCAACG129400.038.302523
TCAACGC128550.038.231794
CAACGCA129800.037.771935
GTACATG184650.037.6694341
TACATGG180700.037.372692
AACGCAG133400.036.9248736
TATCAAC137550.036.1628272
ACATGGG188300.034.7899363
ACGCAGA154100.031.5401617
CGCAGAG157200.029.9745868
CATGGGG69900.029.792544
CATGGGA128650.028.720934
GCAGAGT167600.026.730169
GAGTACT64850.024.27274912-13
ATGGGAT50400.023.7291495
ATGGGAG54900.023.5181985
AGTACAT132400.023.0989282
GAGTACA145400.022.8346141
Produced by FastQC (version 0.11.5)