##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364534_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3009052 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.87632583285367 31.0 28.0 33.0 15.0 34.0 2 27.879705634864404 31.0 28.0 34.0 15.0 34.0 3 27.973152341667742 31.0 28.0 34.0 16.0 34.0 4 31.221423890314956 35.0 32.0 37.0 16.0 37.0 5 31.382803620542283 35.0 32.0 37.0 16.0 37.0 6 31.30771618436637 35.0 32.0 37.0 15.0 37.0 7 31.134391163728644 35.0 32.0 37.0 11.0 37.0 8 31.05701995179877 35.0 32.0 37.0 11.0 37.0 9 32.04336747919278 37.0 32.0 39.0 10.0 39.0 10-11 31.971748743458072 37.0 31.5 39.0 10.0 39.0 12-13 31.89082741009461 37.0 31.0 39.0 8.0 39.0 14-15 32.54563297676478 38.0 31.0 40.0 8.0 41.0 16-17 32.67061818805391 38.0 31.0 40.0 8.0 41.0 18-19 32.46940232338956 38.0 31.0 40.0 8.0 41.0 20-21 32.18212098029545 38.0 30.0 40.0 7.5 41.0 22-23 32.08449289011955 38.0 30.0 40.0 4.5 41.0 24-25 31.908297696417343 38.0 30.0 40.0 2.0 41.0 26-27 31.81669625516608 38.0 30.0 40.0 2.0 41.0 28-29 31.87599233911544 38.0 30.0 40.0 2.0 41.0 30-31 31.812182042716444 37.5 30.0 40.0 2.0 41.0 32-33 31.78962527068326 37.0 30.0 40.0 2.0 41.0 34-35 31.2965478828548 37.0 29.5 40.0 2.0 41.0 36-37 31.16122984913521 37.0 29.5 40.0 2.0 41.0 38-39 30.950210896986825 37.0 29.0 40.0 2.0 41.0 40-41 31.158732384817544 37.0 29.5 40.0 2.0 41.0 42-43 31.033304343028966 37.0 29.0 40.0 2.0 41.0 44-45 30.861047432879193 37.0 29.0 40.0 2.0 41.0 46-47 30.802891907484486 37.0 29.0 40.0 2.0 41.0 48-49 30.644327848106315 36.5 28.5 40.0 2.0 41.0 50-51 30.480512633214715 36.0 28.0 40.0 2.0 41.0 52-53 30.410289685921015 36.0 28.0 40.0 2.0 41.0 54-55 30.43004225251009 36.0 28.0 40.0 2.0 41.0 56-57 30.30201139760961 36.0 28.0 40.0 2.0 41.0 58-59 30.103771885630422 36.0 28.0 40.0 2.0 41.0 60-61 29.89587036049892 35.0 28.0 39.5 2.0 41.0 62-63 29.612468644609663 35.0 28.0 39.0 2.0 41.0 64-65 29.328180603060368 35.0 27.0 39.0 2.0 41.0 66-67 29.08339138040818 35.0 27.0 39.0 2.0 40.0 68-69 29.043643978236332 35.0 28.0 38.5 2.0 40.0 70-71 28.812790041514738 35.0 27.0 37.5 2.0 40.0 72-73 28.534570190212733 34.0 26.5 37.0 2.0 40.0 74-75 28.230058171144933 34.0 26.0 37.0 2.0 39.0 76-77 27.92463706177228 34.0 26.0 36.0 2.0 39.0 78-79 27.61316620649959 34.0 26.0 36.0 2.0 38.5 80-81 27.31794399033317 34.0 26.0 35.0 2.0 37.0 82-83 27.073399196823452 34.0 26.0 35.0 2.0 37.0 84-85 26.828121448216912 34.0 25.0 35.0 2.0 36.5 86-87 26.628435135052502 33.5 25.0 35.0 2.0 36.0 88-89 26.432142581783232 33.0 25.0 35.0 2.0 36.0 90-91 26.28763344734488 33.0 24.0 35.0 2.0 35.5 92-93 26.158655450288 33.0 24.0 35.0 2.0 35.0 94-95 26.03162208562697 33.0 24.0 35.0 2.0 35.0 96-97 25.915756523981642 33.0 23.0 35.0 2.0 35.0 98-99 25.820533676387114 33.0 23.0 35.0 2.0 35.0 100-101 25.719772041161136 33.0 22.0 35.0 2.0 35.0 102-103 24.59526638290066 31.5 19.0 34.0 2.0 35.0 104-105 25.428430449191307 33.0 20.0 35.0 2.0 35.0 106-107 25.567253905881323 33.0 20.0 35.0 2.0 35.0 108-109 25.515233701511306 33.0 20.0 35.0 2.0 35.0 110-111 25.42214491474391 33.0 19.0 35.0 2.0 35.0 112-113 25.316628293562225 33.0 18.5 35.0 2.0 35.0 114-115 25.182454474033683 33.0 18.0 35.0 2.0 35.0 116-117 25.063478131983096 33.0 16.0 35.0 2.0 35.0 118-119 24.90764450066001 33.0 4.5 35.0 2.0 35.0 120-121 24.71343200449843 33.0 2.0 35.0 2.0 35.0 122-123 24.45471696733722 33.0 2.0 35.0 2.0 35.0 124-125 23.035590112766414 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7407054823340466 1101 2 0.7359247122336896 1101 3 0.7901033319641577 1101 4 0.8538901594534529 1101 5 0.7475002278749052 1101 6 0.8207266124520771 1101 7 0.947898519892874 1101 8 0.8822360015836104 1101 9 1.2242985095887562 1101 10-11 1.293201923367672 1101 12-13 0.9927155398111616 1101 14-15 1.2221437221088678 1101 16-17 1.0829471909996968 1101 18-19 1.4602609516962417 1101 20-21 1.8049889029546193 1101 22-23 1.645264727387989 1101 24-25 1.8993319316150874 1101 26-27 1.9203549312979682 1101 28-29 1.6163816704312453 1101 30-31 1.6695562317708372 1101 32-33 1.5830295572330826 1101 34-35 1.9345660825613251 1101 36-37 1.8693217442043775 1101 38-39 1.5909159636548402 1101 40-41 1.5132185411504793 1101 42-43 1.6172369590326099 1101 44-45 1.400860589119553 1101 46-47 1.2156245653196116 1101 48-49 1.2261483537266855 1101 50-51 1.1975738051432003 1101 52-53 0.9353053727945024 1101 54-55 0.8890213877647248 1101 56-57 0.8730284973357776 1101 58-59 0.9676109915630597 1101 60-61 0.9614317755527431 1101 62-63 0.8475647358420204 1101 64-65 0.9439152561754085 1101 66-67 0.9614650596015615 1101 68-69 0.771433206018699 1101 70-71 0.8302043358072382 1101 72-73 0.7020718157808759 1101 74-75 0.7305893800028649 1101 76-77 0.8576054980782004 1101 78-79 0.8738844696090347 1101 80-81 0.9774683188087785 1101 82-83 0.8286386079589398 1101 84-85 0.8715566237386128 1101 86-87 0.9633652302700249 1101 88-89 0.9283040527435276 1101 90-91 0.9110719051947704 1101 92-93 0.8574459890108166 1101 94-95 0.9026184670542712 1101 96-97 0.7620507526837343 1101 98-99 0.8600105658774027 1101 100-101 0.9126926004295832 1101 102-103 0.8962317544338347 1101 104-105 0.996924065125814 1101 106-107 0.8769649075417405 1101 108-109 0.9466641825218467 1101 110-111 0.8432433095634728 1101 112-113 0.878565640667567 1101 114-115 1.0307587453904823 1101 116-117 1.040917264313162 1101 118-119 0.9102515582206756 1101 120-121 1.0438045652369219 1101 122-123 1.1049135757768305 1101 124-125 1.3319607933896798 1102 1 -0.04172037669598794 1102 2 0.1462603809489238 1102 3 0.2863420555747531 1102 4 0.30612693317684503 1102 5 0.43323362528458986 1102 6 -0.25636312941749395 1102 7 -0.8654912204012248 1102 8 0.10262201252279013 1102 9 0.23112177614202523 1102 10-11 0.2637170875609236 1102 12-13 0.42728599158548164 1102 14-15 0.6133247558930108 1102 16-17 0.5235068321034788 1102 18-19 0.6392121428357456 1102 20-21 0.9841492827213045 1102 22-23 0.7617972346210351 1102 24-25 0.9101851298018246 1102 26-27 0.526926342570011 1102 28-29 0.9593581893038916 1102 30-31 0.864070814787322 1102 32-33 0.6073054520615102 1102 34-35 0.9927313330300862 1102 36-37 1.0817669795691316 1102 38-39 1.088465192488659 1102 40-41 0.8829437878751634 1102 42-43 0.7824878911653919 1102 44-45 0.8539971880059483 1102 46-47 0.5675750343129913 1102 48-49 0.1462416962355526 1102 50-51 0.5534681465058782 1102 52-53 0.36812490597692715 1102 54-55 0.12337160196296182 1102 56-57 0.07005094976789294 1102 58-59 0.34339802322741164 1102 60-61 0.37274164131676457 1102 62-63 0.220075903466725 1102 64-65 0.1930042327533883 1102 66-67 0.2192292116009078 1102 68-69 0.1515294564708789 1102 70-71 0.151278141241324 1102 72-73 0.2700019481180398 1102 74-75 -0.022302293651343064 1102 76-77 0.14909468735616827 1102 78-79 0.07303175951193097 1102 80-81 -0.19665789004972112 1102 82-83 -0.056370331598362355 1102 84-85 -0.4747533465424958 1102 86-87 -0.007327947627008058 1102 88-89 0.26717052738021607 1102 90-91 -0.250738741564156 1102 92-93 0.15680835241908753 1102 94-95 0.046242420351713776 1102 96-97 0.3011839569788002 1102 98-99 0.2653788670917301 1102 100-101 0.12585982745541813 1102 102-103 -0.07236711733770562 1102 104-105 0.20794042838230098 1102 106-107 0.14186924969628123 1102 108-109 -0.2935762129502031 1102 110-111 -0.04505583295365412 1102 112-113 -0.28001535095963703 1102 114-115 -0.2215098674289102 1102 116-117 -0.3756285984527139 1102 118-119 0.013601191757004472 1102 120-121 0.4635189947008129 1102 122-123 0.29937718740741204 1102 124-125 0.5640334104181797 1103 1 0.7708006361291346 1103 2 0.7688966086709534 1103 3 0.5840843012051415 1103 4 0.6505265834410565 1103 5 0.8014944743896173 1103 6 0.8778188895045247 1103 7 0.9912709474210857 1103 8 1.0663475306829646 1103 9 1.2849313929700372 1103 10-11 1.3196458268861484 1103 12-13 1.1697458562528489 1103 14-15 1.5209930250520003 1103 16-17 1.439995210473036 1103 18-19 1.4391279576710119 1103 20-21 1.7858474999893588 1103 22-23 1.742520757483149 1103 24-25 1.7633505197983084 1103 26-27 1.812698370111864 1103 28-29 1.773717364168796 1103 30-31 1.789604915107855 1103 32-33 1.789159231939884 1103 34-35 2.1640416302488674 1103 36-37 2.0359515295551205 1103 38-39 2.157966196007127 1103 40-41 1.8840970540958253 1103 42-43 1.7726917056579623 1103 44-45 1.418010401024837 1103 46-47 1.5194528459126566 1103 48-49 1.465692500661845 1103 50-51 1.4361221565483717 1103 52-53 1.4846525735026255 1103 54-55 1.3550536957974728 1103 56-57 1.3870802787033405 1103 58-59 1.2660177187150836 1103 60-61 1.198984331427937 1103 62-63 1.1742963136247155 1103 64-65 1.341127028691453 1103 66-67 1.3725073255896127 1103 68-69 1.4778948125688238 1103 70-71 1.5608969669203177 1103 72-73 1.4816690718109733 1103 74-75 1.4166925001621955 1103 76-77 1.4922591825216607 1103 78-79 1.520930276203412 1103 80-81 1.4120777456731304 1103 82-83 1.4753524827099938 1103 84-85 1.4278743931566282 1103 86-87 1.4018471953065408 1103 88-89 1.3611431764434592 1103 90-91 1.3638269394943912 1103 92-93 1.4080102731444732 1103 94-95 1.3505051676517503 1103 96-97 1.4670739879127694 1103 98-99 1.4039362131444975 1103 100-101 1.4308382078648556 1103 102-103 1.3863844430817664 1103 104-105 1.315578634720854 1103 106-107 1.385373847572719 1103 108-109 1.5687044569188338 1103 110-111 1.5172862394152133 1103 112-113 1.563230389505808 1103 114-115 1.6144055699091844 1103 116-117 1.704449019126173 1103 118-119 1.7437324168177106 1103 120-121 1.7394230649049902 1103 122-123 1.722970668053886 1103 124-125 1.8924830328231828 1104 1 0.6157046528650767 1104 2 0.6269639373163685 1104 3 0.5833102744222849 1104 4 0.747163757557125 1104 5 0.6354021849312055 1104 6 0.5722978671063608 1104 7 0.7467836943636144 1104 8 0.6181493825193272 1104 9 1.0393719370670595 1104 10-11 0.9537132330448408 1104 12-13 0.9204711727709913 1104 14-15 0.9194580169895588 1104 16-17 1.0568080512104743 1104 18-19 1.3302943679957053 1104 20-21 1.5676293849448513 1104 22-23 1.3530864226225319 1104 24-25 1.7582033402668955 1104 26-27 1.7554835239925417 1104 28-29 1.5945876978288211 1104 30-31 1.5061057889594274 1104 32-33 1.451730125089945 1104 34-35 1.7474931568997292 1104 36-37 1.755340277762862 1104 38-39 1.8177333157417657 1104 40-41 1.4560438461378098 1104 42-43 1.5181244090853525 1104 44-45 1.3485724520837792 1104 46-47 1.2788282495274466 1104 48-49 1.2046339241883182 1104 50-51 1.0004422098190346 1104 52-53 1.1069256762330077 1104 54-55 0.9903075791788503 1104 56-57 0.9893067753755069 1104 58-59 1.0543129065736956 1104 60-61 1.0121492349561265 1104 62-63 0.9452363178696714 1104 64-65 0.9854587647320159 1104 66-67 0.8751394830773513 1104 68-69 0.8998888098100792 1104 70-71 0.9370986991592716 1104 72-73 0.8596356255063711 1104 74-75 0.9288811499684719 1104 76-77 0.8713019782158113 1104 78-79 0.9449595979555312 1104 80-81 0.9224619758956294 1104 82-83 0.9386422056904387 1104 84-85 0.8808217680868893 1104 86-87 0.921074213895011 1104 88-89 0.9868191455685569 1104 90-91 1.0536774802456499 1104 92-93 0.9692587427141213 1104 94-95 1.0179941866847209 1104 96-97 0.919081490351406 1104 98-99 1.028279766593414 1104 100-101 1.1150441281287051 1104 102-103 1.0405607496204183 1104 104-105 1.0487147371630137 1104 106-107 1.0303593734594152 1104 108-109 1.0129312759539069 1104 110-111 1.062847815873642 1104 112-113 1.060145390045804 1104 114-115 1.044476262784567 1104 116-117 1.003007887717036 1104 118-119 1.0276347850982788 1104 120-121 1.00382007366197 1104 122-123 1.000530195315239 1104 124-125 1.1505801886918938 1105 1 0.21519017873947988 1105 2 0.4946571304672318 1105 3 0.5212019786783699 1105 4 0.6468007195488177 1105 5 0.5547195985521682 1105 6 0.47220973749188033 1105 7 0.5800699167609125 1105 8 0.37882270563961384 1105 9 0.30945041934568707 1105 10-11 0.5490830321346714 1105 12-13 0.5520775591975955 1105 14-15 0.6832559871325827 1105 16-17 0.5786607616123547 1105 18-19 0.6447447300025644 1105 20-21 1.1012327263778587 1105 22-23 1.2310344272851879 1105 24-25 1.3461797496075718 1105 26-27 1.060636476552208 1105 28-29 0.7889818937615019 1105 30-31 0.7007849682150287 1105 32-33 0.8401066273659339 1105 34-35 1.5513364632776643 1105 36-37 1.4744675259147115 1105 38-39 1.6317418944729418 1105 40-41 1.2989242390392306 1105 42-43 0.8241026544281311 1105 44-45 0.9640395116064582 1105 46-47 0.9830851388396447 1105 48-49 0.8298975987997039 1105 50-51 0.8662420258025847 1105 52-53 0.9234663258757827 1105 54-55 0.6632500279674645 1105 56-57 0.7981795525765385 1105 58-59 0.7186289929528265 1105 60-61 0.706916014064678 1105 62-63 0.7655440449902642 1105 64-65 0.8193899244916345 1105 66-67 0.7070334732544374 1105 68-69 0.4828376876332605 1105 70-71 0.6913579345582406 1105 72-73 0.7619381393951059 1105 74-75 0.735888078681679 1105 76-77 0.855176557552042 1105 78-79 0.845484620011586 1105 80-81 0.7104321132382019 1105 82-83 0.7161090166035997 1105 84-85 0.7335200138383442 1105 86-87 0.7709855828990797 1105 88-89 0.9546859869808486 1105 90-91 0.7097283880582488 1105 92-93 0.5969429545150469 1105 94-95 0.6239900560583926 1105 96-97 0.774466525761234 1105 98-99 0.6813716251614608 1105 100-101 0.7191586818698887 1105 102-103 0.6550873405274018 1105 104-105 0.7842069811704171 1105 106-107 0.7182891070253099 1105 108-109 0.7267521109829467 1105 110-111 0.7661377484936516 1105 112-113 0.856636173992591 1105 114-115 0.7496196512566158 1105 116-117 0.7485691482997332 1105 118-119 0.7045112808468446 1105 120-121 0.6329775901172141 1105 122-123 0.8877053687222691 1105 124-125 1.0692700241006605 1106 1 0.33822337221635834 1106 2 0.5216428002534776 1106 3 0.2746783631519669 1106 4 0.4843770128652025 1106 5 0.40527778861750363 1106 6 0.30975189133128467 1106 7 0.1065630164032747 1106 8 0.3285258316328452 1106 9 0.4526572548575487 1106 10-11 0.6369516988169188 1106 12-13 0.6008052914833222 1106 14-15 0.8842136667790754 1106 16-17 0.7178929686218396 1106 18-19 0.6388285028936949 1106 20-21 1.0945244393612228 1106 22-23 0.8610521009558383 1106 24-25 1.141701568628143 1106 26-27 1.3277568232092598 1106 28-29 1.1407418895839854 1106 30-31 0.9461933528128732 1106 32-33 0.881736858555584 1106 34-35 1.2132599785397886 1106 36-37 1.1000302863424167 1106 38-39 1.2449383025383618 1106 40-41 1.0828783771237056 1106 42-43 1.0236966543044588 1106 44-45 0.8890704463001384 1106 46-47 0.6784364672355423 1106 48-49 0.8180119639318946 1106 50-51 0.7488083075971375 1106 52-53 0.7238375614334664 1106 54-55 0.5239479792814912 1106 56-57 0.5447688745751798 1106 58-59 0.4948532081511665 1106 60-61 0.5441901489679282 1106 62-63 0.5893998748444957 1106 64-65 0.6604499590984858 1106 66-67 0.5873957873493545 1106 68-69 0.5959219403042404 1106 70-71 0.6524460472019697 1106 72-73 0.6457614030733296 1106 74-75 0.7484149827533066 1106 76-77 0.6170907541794648 1106 78-79 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-2.5937243537390486 2216 74-75 -2.42625754447349 2216 76-77 -2.518853393471243 2216 78-79 -2.702807018377701 2216 80-81 -2.635831494226501 2216 82-83 -2.384505927606334 2216 84-85 -2.4394279284995264 2216 86-87 -2.4704454687166866 2216 88-89 -2.618686611206151 2216 90-91 -2.6153323514492364 2216 92-93 -2.552836029254056 2216 94-95 -2.5147867009709124 2216 96-97 -2.527451524127155 2216 98-99 -2.5489335486229407 2216 100-101 -2.524537322792895 2216 102-103 -2.3938550707088417 2216 104-105 -2.5934171827607493 2216 106-107 -2.6134780783298446 2216 108-109 -2.6050150106126218 2216 110-111 -2.6727345969078407 2216 112-113 -2.661535563218955 2216 114-115 -2.8108500681680475 2216 116-117 -2.884801518396859 2216 118-119 -2.92806606782041 2216 120-121 -2.990125402783285 2216 122-123 -2.9624493375385903 2216 124-125 -3.0722843130517 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 225676.0 3 36998.0 4 22982.0 5 18511.0 6 16918.0 7 17820.0 8 18919.0 9 19558.0 10 21065.0 11 22764.0 12 24717.0 13 26960.0 14 30460.0 15 26753.0 16 20418.0 17 18219.0 18 16265.0 19 15634.0 20 15310.0 21 16052.0 22 19662.0 23 25919.0 24 35170.0 25 45390.0 26 53640.0 27 59112.0 28 68400.0 29 78732.0 30 89048.0 31 101244.0 32 122596.0 33 152364.0 34 194979.0 35 263471.0 36 394229.0 37 508338.0 38 164449.0 39 310.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.84846812933264 18.82247091524698 12.95955372191443 23.369507233505953 2 15.008474745259129 20.39904418167195 41.00640092524277 23.586080147826145 3 19.019872356675453 26.44107239536129 28.833312893650838 25.70574235431242 4 12.061880099745718 16.712492576069735 37.76308630769686 33.46254101648769 5 13.166102627816123 39.1332946449879 33.78017707562641 13.920425651569571 6 30.889461463072898 37.24620915160835 18.00072648165202 13.863602903666738 7 28.24680881894977 33.546062118976785 20.618517640857487 17.58861142121596 8 26.286119349216964 35.57349623735316 19.766424774314302 18.373959639115576 9 27.29692157423812 16.6177297037893 19.407950819100073 36.677397902872514 10-11 24.609760815034104 27.592278232479867 27.15232239256749 20.645638559918538 12-13 25.525102963401775 25.024547538474707 27.441331424588082 22.009018073535437 14-15 22.709581283665887 26.09852729742383 25.602900964644864 25.588990454265414 16-17 22.226709975144498 29.57053534238397 25.880538676618176 22.32221600585336 18-19 22.073065676885967 28.01520631251076 28.387354505759948 21.52437350484333 20-21 23.263991076899096 27.1091037531497 28.2343955827194 21.392509587231803 22-23 22.764933387061262 27.150744228748042 27.883366082444677 22.200956301746018 24-25 22.343159244843793 27.086408760378344 28.29381248590559 22.27661950887227 26-27 22.28138968736621 27.571776537441867 27.894628027750667 22.252205747441252 28-29 22.204038131091956 27.412774936063506 28.132157077978842 22.251029854865696 30-31 22.39974130970602 27.198173747554634 28.033217641654456 22.368867301084894 32-33 22.370379996440445 27.207999057432943 27.78577006249492 22.63585088363169 34-35 22.556320132199676 27.037369274344236 28.09186574500911 22.31444484844698 36-37 22.54789803688261 26.883225009846328 27.943421404676716 22.625455548594342 38-39 22.394568918904998 27.062867993928986 28.12232162233209 22.42024146483393 40-41 22.64236648389729 27.041064106676593 27.8268449928449 22.48972441658121 42-43 22.404762336040026 27.062338150520038 27.75720554757243 22.77569396586751 44-45 22.561810824061222 27.184597359214912 27.561071271282916 22.692520545440946 46-47 22.621914671901735 27.467510552309392 27.04091492730742 22.86965984848145 48-49 22.60609707046767 27.38592508135743 27.116789049073738 22.891188799101162 50-51 22.73195424027247 27.506070256110636 27.033743511821797 22.7282319917951 52-53 22.874031422521114 27.47868763982809 26.87356350106279 22.773717436588 54-55 22.66318880658655 27.42289903292444 27.006203974997966 22.907708185491042 56-57 22.709946211588576 27.721695445823656 26.699900080410927 22.868458262176837 58-59 22.75060164467546 27.268033845306654 26.834972641638448 23.14639186837944 60-61 22.848284465194528 27.27425907643929 27.128177270960563 22.749279187405623 62-63 22.627953781616657 27.317521769474183 27.104443447567363 22.950081001341797 64-65 22.51355173324412 27.28062744894698 27.031388900383856 23.174431917425043 66-67 22.58542544727514 27.626821287352136 26.72660467039194 23.06114859498079 68-69 22.66432193492555 27.41556148663438 27.02690574686969 22.89321083157038 70-71 22.448748649662285 27.360895482656105 27.17983681788732 23.01051904979429 72-73 22.707715818981075 27.353589687513086 26.91155521550489 23.027139278000945 74-75 22.58632372797935 27.582998759409527 26.73376091000119 23.096916602609927 76-77 22.752881713404324 27.40091269628936 26.870832969134433 22.975372621171882 78-79 22.699331759732605 27.539990146301662 26.81541929314254 22.94525880082319 80-81 22.59775267754607 27.718710643566535 26.89440548947418 22.789131189413215 82-83 22.761207980510502 27.63211964883414 26.733517103906923 22.873155266748434 84-85 22.727058222988504 27.467637648003425 26.7657886935819 23.039515435426175 86-87 22.63082346505331 27.382370800061018 26.923213697574816 23.06359203731086 88-89 22.853544565433985 27.320233978229858 26.801017293761277 23.025204162574884 90-91 22.664588717881557 27.611860795159444 26.885132721859726 22.83841776509927 92-93 22.660301367418224 27.358334896741137 26.891190306417407 23.09017342942323 94-95 22.6311385931928 27.31487821871435 26.900530657398225 23.15345253069463 96-97 22.76837760971207 27.315425344240264 26.76481043746887 23.151386608578797 98-99 22.54716147162668 27.31987182554875 26.699820273949292 23.433146428875276 100-101 22.465512032525893 27.432935954929583 26.916551199929007 23.185000812615513 102-103 22.630050926338264 27.131884061824124 26.900017015325755 23.338047996511857 104-105 22.55955903111671 27.18390314689829 27.044338994496698 23.212198827488308 106-107 22.476937080827145 27.377784557323885 26.828325608306407 23.316952753542562 108-109 22.524369801518883 27.40469423592547 26.58240867887959 23.488527283676056 110-111 22.34418496758667 27.35705541779404 26.923443129160297 23.375316485458992 112-113 22.38977591613571 27.41797084264413 26.895763183886483 23.29649005733367 114-115 22.296823052576027 27.59752905566271 26.774811468861287 23.33083642289997 116-117 22.184079902906298 27.62423181786157 26.882370261464406 23.309318017767723 118-119 22.301093500544358 27.655769990016786 26.599922500508466 23.443214008930386 120-121 22.127814426480118 27.714245359831175 26.84564895897376 23.312291254714943 122-123 22.38999193101349 27.69422063826082 26.53691262231427 23.37887480841142 124-125 22.392487942673974 27.775811021172164 26.35702050667861 23.474680529475258 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1499.0 1 1576.0 2 1763.5 3 1672.5 4 1258.0 5 953.5 6 851.0 7 977.5 8 1350.0 9 1597.0 10 1543.0 11 1477.5 12 1543.5 13 1746.5 14 1995.0 15 2186.0 16 2349.0 17 2582.0 18 2844.0 19 3131.5 20 3638.0 21 4325.5 22 5188.5 23 6371.0 24 7861.5 25 10001.0 26 13509.5 27 18073.5 28 22440.5 29 27436.0 30 33751.5 31 40659.0 32 47915.0 33 54677.5 34 61291.0 35 66457.5 36 71882.0 37 78851.5 38 83515.5 39 86143.0 40 89266.5 41 94929.5 42 98991.5 43 102047.5 44 105949.0 45 107658.5 46 107539.5 47 107101.0 48 110997.5 49 115609.0 50 115092.5 51 111889.5 52 108814.5 53 106183.0 54 101506.5 55 93202.5 56 84163.0 57 76440.0 58 72392.5 59 67069.5 60 56989.0 61 46706.0 62 37097.0 63 27654.0 64 20173.0 65 15607.0 66 11818.0 67 9014.0 68 6910.0 69 5020.5 70 3769.0 71 3049.0 72 2450.5 73 1974.0 74 1553.0 75 1244.0 76 921.0 77 577.0 78 397.0 79 268.5 80 193.5 81 145.5 82 109.0 83 88.0 84 69.0 85 48.0 86 34.5 87 34.5 88 32.5 89 23.0 90 19.0 91 15.5 92 12.0 93 9.0 94 8.0 95 8.5 96 6.5 97 6.0 98 6.0 99 6.5 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011465405051158969 2 0.0037221025093617526 3 0.006613378565740971 4 0.006679844681979574 5 0.013093824899004737 6 9.637586854597394E-4 7 0.00707864137941119 8 0.0 9 0.0026254115914248074 10-11 0.0 12-13 0.0031405239922739784 14-15 0.017796302622885878 16-17 0.028746595273195678 18-19 0.005649619880281231 20-21 0.007726686012737567 22-23 0.0021435322486949375 24-25 0.01075089430159399 26-27 0.01821171584937715 28-29 3.821801683719657E-4 30-31 0.0016118033187861162 32-33 0.008225181884527087 34-35 0.008972925692211368 36-37 0.010534879423818533 38-39 3.323305811930136E-5 40-41 0.023495772090346062 42-43 0.009188940569986826 44-45 0.015852168722906748 46-47 0.0037719520965407045 48-49 0.023994267962135583 50-51 0.003921500858077561 52-53 0.0 54-55 0.016516829885292777 56-57 0.00440338020080743 58-59 2.8248099401406154E-4 60-61 0.004037816561495116 62-63 0.025539605164683097 64-65 0.016234348891278713 66-67 0.0017945851384422735 68-69 0.020122616691236973 70-71 0.0027417272948423626 72-73 0.011631570341755477 74-75 3.323305811930136E-5 76-77 0.005483454589684725 78-79 9.803752145193902E-4 80-81 0.0031072909341546776 82-83 0.0015453372025475132 84-85 0.0 86-87 0.00561638682216193 88-89 0.016001717484443605 90-91 0.021152841492935316 92-93 0.004436613258926732 94-95 0.023529005148465364 96-97 0.016832543937426142 98-99 9.305256273404381E-4 100-101 0.00827503147170604 102-103 0.0 104-105 0.0013791719119510065 106-107 3.323305811930136E-5 108-109 0.0 110-111 9.139090982807874E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 6.646611623860272E-5 122-123 0.0 124-125 0.023761636555300475 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3009052.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.80238068712639 #Duplication Level Percentage of deduplicated Percentage of total 1 75.22899316056044 37.46582956091458 2 11.228196322527108 11.183818153685749 3 4.7409484164922935 7.083315535685347 4 2.575783998621487 5.131207010686237 5 1.5997359930954709 3.9835330463519405 6 1.0118014772035526 3.023407340849289 7 0.7062580880878121 2.462133391641788 8 0.49227741972288397 1.961326996857229 9 0.3872032563104395 1.7355279576660734 >10 1.859048296193264 16.125902977855 >50 0.11114167215484716 3.8043646712583397 >100 0.05649557996346187 5.016187420175697 >500 0.0018432456389762884 0.5806734817819365 >1k 2.0480507095515168E-4 0.26182835543300265 >5k 6.826835698505056E-5 0.18094409915782886 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5322 0.17686633531092186 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3749 0.1245907348892608 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.323305811930136E-5 0.0 4 0.0 0.0 0.0 3.323305811930136E-5 0.0 5 0.0 0.0 0.0 3.323305811930136E-5 0.0 6 0.0 0.0 0.0 3.323305811930136E-5 0.0 7 0.0 0.0 0.0 6.646611623860272E-5 3.323305811930136E-5 8 0.0 0.0 0.0 6.646611623860272E-5 3.323305811930136E-5 9 0.0 0.0 0.0 1.9939834871580817E-4 3.323305811930136E-5 10-11 1.661652905965068E-5 0.0 0.0 2.3263140683510954E-4 3.323305811930136E-5 12-13 3.323305811930136E-5 0.0 0.0 2.9909752307371223E-4 2.3263140683510954E-4 14-15 3.323305811930136E-5 0.0 0.0 2.9909752307371223E-4 2.824809940140616E-4 16-17 3.323305811930136E-5 0.0 0.0 3.4894711025266426E-4 3.8218016837196565E-4 18-19 6.646611623860272E-5 0.0 0.0 3.6556363931231496E-4 3.9879669743161635E-4 20-21 9.969917435790409E-5 0.0 0.0 4.8187934272986977E-4 3.9879669743161635E-4 22-23 9.969917435790409E-5 0.0 0.0 6.812776914456779E-4 3.9879669743161635E-4 24-25 9.969917435790409E-5 0.0 0.0 0.0010634578598176436 3.9879669743161635E-4 26-27 9.969917435790409E-5 0.0 0.0 0.001345938853831705 3.9879669743161635E-4 28-29 1.3293223247720545E-4 0.0 0.0 0.002243231423052842 3.9879669743161635E-4 30-31 1.3293223247720545E-4 0.0 0.0 0.005151124008491711 3.9879669743161635E-4 32-33 1.4954876153685614E-4 0.0 0.0 0.010667811656295738 3.9879669743161635E-4 34-35 1.661652905965068E-4 0.0 0.0 0.01856066295962981 4.320297555509177E-4 36-37 1.661652905965068E-4 0.0 0.0 0.03310012588682416 4.320297555509177E-4 38-39 1.661652905965068E-4 0.0 0.0 0.05657928144811057 4.320297555509177E-4 40-41 1.661652905965068E-4 0.0 0.0 0.08647241722642214 4.320297555509177E-4 42-43 1.661652905965068E-4 0.0 0.0 0.11575074142952664 4.320297555509177E-4 44-45 1.661652905965068E-4 0.0 0.0 0.14903364913600697 4.320297555509177E-4 46-47 1.9939834871580817E-4 0.0 0.0 0.18954474698343532 4.320297555509177E-4 48-49 1.9939834871580817E-4 0.0 0.0 0.2366193738094257 4.486462846105684E-4 50-51 1.9939834871580817E-4 0.0 0.0 0.29137083706097466 4.6526281367021907E-4 52-53 1.9939834871580817E-4 0.0 0.0 0.34132012341428464 4.984958717895204E-4 54-55 1.9939834871580817E-4 0.0 0.0 0.3968193304735179 5.317289299088218E-4 56-57 2.3263140683510954E-4 0.0 0.0 0.4624213872010188 5.317289299088218E-4 58-59 2.3263140683510954E-4 0.0 0.0 0.5392396010437839 5.483454589684725E-4 60-61 2.3263140683510954E-4 0.0 0.0 0.6281546480419746 5.649619880281232E-4 62-63 2.3263140683510954E-4 0.0 0.0 0.7182660851324603 6.314281042667259E-4 64-65 2.3263140683510954E-4 0.0 0.0 0.8077460941186793 6.314281042667259E-4 66-67 2.3263140683510954E-4 0.0 0.0 0.9152218040764999 6.314281042667259E-4 68-69 2.3263140683510954E-4 0.0 0.0 1.042653965434961 6.314281042667259E-4 70-71 2.492479358947602E-4 0.0 0.0 1.1850410029471075 6.314281042667259E-4 72-73 2.658644649544109E-4 0.0 0.0 1.3367499132617184 7.145107495649793E-4 74-75 2.658644649544109E-4 0.0 0.0 1.4832080003934793 8.308264529825341E-4 76-77 2.658644649544109E-4 0.0 0.0 1.6453188578994316 8.640595111018354E-4 78-79 2.658644649544109E-4 0.0 0.0 1.838901421444362 8.972925692211368E-4 80-81 2.658644649544109E-4 0.0 0.0 2.0631082480462286 8.972925692211368E-4 82-83 2.658644649544109E-4 0.0 0.0 2.2973348416710646 9.305256273404381E-4 84-85 2.990975230737123E-4 0.0 0.0 2.5333394039052832 9.305256273404381E-4 86-87 3.323305811930136E-4 0.0 0.0 2.774744338083888 9.969917435790408E-4 88-89 3.323305811930136E-4 0.0 0.0 3.0562449568834302 0.0010634578598176436 90-91 3.6556363931231496E-4 0.0 0.0 3.3739197594458323 0.0010634578598176436 92-93 3.6556363931231496E-4 0.0 0.0 3.7138773274772254 0.0010800743888772944 94-95 3.6556363931231496E-4 0.0 0.0 4.061428649288879 0.0011133074469965956 96-97 3.6556363931231496E-4 3.323305811930136E-5 0.0 4.413532900062878 0.0012296231504141503 98-99 3.6556363931231496E-4 3.323305811930136E-5 0.0 4.795214572563053 0.0013293223247720545 100-101 3.8218016837196565E-4 3.323305811930136E-5 0.0 5.242448452203551 0.0013293223247720545 102-103 4.320297555509177E-4 3.323305811930136E-5 0.0 5.718445543646304 0.0013625553828913559 104-105 5.317289299088218E-4 3.323305811930136E-5 0.0 6.19432631938564 0.0013957884410106573 106-107 5.317289299088218E-4 3.323305811930136E-5 0.0 6.671187470339495 0.0013957884410106573 108-109 5.317289299088218E-4 6.646611623860272E-5 0.0 7.183441828190407 0.0013957884410106573 110-111 5.317289299088218E-4 6.646611623860272E-5 0.0 7.743020060803203 0.0013957884410106573 112-113 5.317289299088218E-4 6.646611623860272E-5 0.0 8.352348181420595 0.0013957884410106573 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 10400 0.0 48.51616 1 GGTATCA 6185 0.0 45.984562 1 ATCAACG 12940 0.0 38.30252 3 TCAACGC 12855 0.0 38.23179 4 CAACGCA 12980 0.0 37.77193 5 GTACATG 18465 0.0 37.669434 1 TACATGG 18070 0.0 37.37269 2 AACGCAG 13340 0.0 36.924873 6 TATCAAC 13755 0.0 36.162827 2 ACATGGG 18830 0.0 34.789936 3 ACGCAGA 15410 0.0 31.540161 7 CGCAGAG 15720 0.0 29.974586 8 CATGGGG 6990 0.0 29.79254 4 CATGGGA 12865 0.0 28.72093 4 GCAGAGT 16760 0.0 26.73016 9 GAGTACT 6485 0.0 24.272749 12-13 ATGGGAT 5040 0.0 23.729149 5 ATGGGAG 5490 0.0 23.518198 5 AGTACAT 13240 0.0 23.098928 2 GAGTACA 14540 0.0 22.834614 1 >>END_MODULE