FastQCFastQC Report
Thu 26 May 2016
SRR1364534_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364534_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3009052
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98170.3262489315571815No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83290.27679814107566103No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52550.17463972041692866No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA76800.060.0764051
GTATCAA131900.050.1199871
ATCAACG173550.037.913353
TCAACGC175850.037.315884
CAACGCA177550.036.8943755
TATCAAC179700.036.5495382
AACGCAG181750.036.146
TACATGG173750.031.6666152
GTACATG179850.031.354861
ACGCAGA212500.030.7983347
CGCAGAG215450.030.2109438
ACATGGG182350.029.4873963
GAGTACT122500.027.73232712-13
GCAGAGT231450.027.2741819
AGTACTT128200.026.01200712-13
CATGGGA123050.025.5993234
GTACTTT134750.025.27785514-15
AGAGTAC216900.024.3030310-11
CAGAGTA222500.023.97212810-11
TGGTATC40900.023.5855582
Produced by FastQC (version 0.11.5)