FastQCFastQC Report
Thu 26 May 2016
SRR1364533_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364533_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3418090
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71440.2090056142465529No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49040.14347193900687227No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA75250.050.210121
GTATCAA134200.048.1505661
TCAACGC159950.039.727574
ATCAACG160200.039.5541533
GTACATG203450.039.0141531
CAACGCA164400.038.6522185
AACGCAG167950.037.5468646
TACATGG207450.037.285072
TATCAAC175000.036.3449972
ACATGGG217900.034.8143233
ACGCAGA193450.032.2289927
CGCAGAG189450.031.9692738
CATGGGA152250.030.481924
GCAGAGT199750.028.4745629
GAGTACT75500.026.56221212-13
CATGGGG76950.026.2117544
ATGGGAT61650.025.0925795
GTATAGG10550.024.251581
ATGGGAG61150.023.5463665
CAGAGTA194700.023.48699410-11
Produced by FastQC (version 0.11.5)