##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364533_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3418090 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.61723974500379 31.0 28.0 31.0 12.0 34.0 2 27.63516583823129 31.0 27.0 33.0 12.0 34.0 3 27.787273009195193 31.0 27.0 34.0 15.0 34.0 4 31.036720507651935 35.0 32.0 37.0 16.0 37.0 5 31.128657817670103 35.0 32.0 37.0 16.0 37.0 6 31.1663938047272 35.0 32.0 37.0 13.0 37.0 7 30.92465821555313 35.0 31.0 37.0 10.0 37.0 8 30.82820756621388 35.0 31.0 37.0 10.0 37.0 9 31.782026219321317 37.0 31.0 39.0 8.0 39.0 10-11 31.718180182499584 37.0 31.0 39.0 8.0 39.0 12-13 31.61844919238522 37.0 31.0 39.0 8.0 39.0 14-15 32.24362582611926 38.0 30.5 40.0 8.0 41.0 16-17 32.36167333218259 38.0 31.0 40.0 7.0 41.0 18-19 32.17900786696664 38.0 30.0 40.0 4.5 41.0 20-21 31.89525992586503 38.0 30.0 40.0 2.0 41.0 22-23 31.799082235985594 38.0 30.0 40.0 2.0 41.0 24-25 31.623792527405655 37.5 29.0 40.0 2.0 41.0 26-27 31.519966852833015 37.0 29.5 40.0 2.0 41.0 28-29 31.576981296572065 37.0 30.0 40.0 2.0 41.0 30-31 31.508880105556027 37.0 30.0 40.0 2.0 41.0 32-33 31.474019701061117 37.0 30.0 40.0 2.0 41.0 34-35 30.9818716008063 37.0 29.0 40.0 2.0 41.0 36-37 30.83333937959504 37.0 28.5 40.0 2.0 41.0 38-39 30.61006687945607 36.0 28.0 40.0 2.0 41.0 40-41 30.814429696116836 37.0 29.0 40.0 2.0 41.0 42-43 30.68335093575652 37.0 28.5 40.0 2.0 41.0 44-45 30.50561907381023 36.0 28.0 40.0 2.0 41.0 46-47 30.432268752431916 36.0 28.0 40.0 2.0 41.0 48-49 30.255124791915954 36.0 28.0 40.0 2.0 41.0 50-51 30.086071022120542 36.0 28.0 40.0 2.0 41.0 52-53 30.002606865237603 36.0 27.5 40.0 2.0 41.0 54-55 30.011401835528027 36.0 28.0 40.0 2.0 41.0 56-57 29.875514688027526 36.0 28.0 40.0 2.0 41.0 58-59 29.671971627429354 35.0 27.5 40.0 2.0 41.0 60-61 29.461681670172524 35.0 27.0 39.0 2.0 41.0 62-63 29.1754737294805 35.0 26.5 39.0 2.0 41.0 64-65 28.8914582412985 35.0 26.0 39.0 2.0 40.5 66-67 28.642393705256445 34.0 26.0 38.5 2.0 40.0 68-69 28.594572114836062 34.0 26.0 38.0 2.0 40.0 70-71 28.361138969424445 34.0 26.0 37.5 2.0 40.0 72-73 28.081884473492508 34.0 26.0 37.0 2.0 39.5 74-75 27.7782036166397 34.0 26.0 36.5 2.0 39.0 76-77 27.474317381929673 34.0 26.0 36.0 2.0 39.0 78-79 27.17430626460977 34.0 25.5 36.0 2.0 38.5 80-81 26.88040762531121 34.0 25.0 35.0 2.0 37.0 82-83 26.636862546041797 33.5 24.5 35.0 2.0 37.0 84-85 26.394789780257394 33.0 24.0 35.0 2.0 36.0 86-87 26.20486426630077 33.0 23.5 35.0 2.0 36.0 88-89 26.002761337472098 33.0 23.0 35.0 2.0 36.0 90-91 25.86013489990024 33.0 22.5 35.0 2.0 35.5 92-93 25.734825150888362 33.0 20.5 35.0 2.0 35.0 94-95 25.603452366672617 33.0 20.0 35.0 2.0 35.0 96-97 25.488645851923152 33.0 20.0 35.0 2.0 35.0 98-99 25.386377479820602 33.0 19.5 35.0 2.0 35.0 100-101 25.29081914168439 33.0 19.0 35.0 2.0 35.0 102-103 24.183618482836906 31.0 17.0 34.0 2.0 35.0 104-105 24.99514802126334 32.5 18.0 35.0 2.0 35.0 106-107 25.127967519872207 33.0 18.0 35.0 2.0 35.0 108-109 25.07154668250397 33.0 17.0 35.0 2.0 35.0 110-111 24.98036988493574 33.0 14.0 35.0 2.0 35.0 112-113 24.87664119435123 33.0 8.5 35.0 2.0 35.0 114-115 24.73962578516072 33.0 4.5 35.0 2.0 35.0 116-117 24.618107627359137 33.0 2.0 35.0 2.0 35.0 118-119 24.452695950077384 33.0 2.0 35.0 2.0 35.0 120-121 24.25319900880316 33.0 2.0 35.0 2.0 35.0 122-123 23.995622555286722 33.0 2.0 35.0 2.0 35.0 124-125 22.590072672164865 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6259669442494094 1101 2 0.6612665348847138 1101 3 0.6463053135062111 1101 4 0.7019525437036833 1101 5 0.6418360083002206 1101 6 0.7606931993423771 1101 7 0.8078719456515131 1101 8 0.5859919157082594 1101 9 0.8716762029098852 1101 10-11 0.9435280229806793 1101 12-13 0.802170940418371 1101 14-15 1.0450570031829258 1101 16-17 0.9776863650367815 1101 18-19 1.2816309850150205 1101 20-21 1.5892250558946692 1101 22-23 1.4508399347416727 1101 24-25 1.6522061258600793 1101 26-27 1.71371204205278 1101 28-29 1.4754287458392277 1101 30-31 1.5912979189849779 1101 32-33 1.612146164712513 1101 34-35 1.9572608089029053 1101 36-37 1.8358513906904257 1101 38-39 1.677443579865649 1101 40-41 1.5164746055507834 1101 42-43 1.504171399137622 1101 44-45 1.457896932909545 1101 46-47 1.3912017181116454 1101 48-49 1.4556890074933122 1101 50-51 1.3299147574655592 1101 52-53 1.1315307733984312 1101 54-55 1.1100224719495095 1101 56-57 1.0336479920956663 1101 58-59 1.05450490947403 1101 60-61 1.056342492632492 1101 62-63 1.009341647524522 1101 64-65 1.0821455180896855 1101 66-67 1.0099371505377377 1101 68-69 0.8614384084477642 1101 70-71 0.7016690988608616 1101 72-73 0.6206775626971535 1101 74-75 0.6283907440706784 1101 76-77 0.7890285990378487 1101 78-79 0.8724053682178727 1101 80-81 0.9426483365715121 1101 82-83 0.8285431455411789 1101 84-85 0.8890177245966164 1101 86-87 0.972474557817339 1101 88-89 0.8269096647196186 1101 90-91 0.7807178412201381 1101 92-93 0.7073172886753376 1101 94-95 0.8047623135187898 1101 96-97 0.7574867519304895 1101 98-99 0.8220930144578595 1101 100-101 0.8834009444800586 1101 102-103 0.8577452484873653 1101 104-105 0.8737542899478647 1101 106-107 0.8412028036168699 1101 108-109 0.8835065884760276 1101 110-111 0.749083117067201 1101 112-113 0.7533023532015157 1101 114-115 0.9570910860215989 1101 116-117 0.9514339242699954 1101 118-119 0.9342357987592003 1101 120-121 1.0212212807260066 1101 122-123 1.0291995184802225 1101 124-125 1.2531684694555345 1102 1 -0.20962984355891123 1102 2 0.058443476816009365 1102 3 0.18387483751516953 1102 4 0.29568389554011887 1102 5 0.3587426822238413 1102 6 -0.47513977741222746 1102 7 -1.0720112718178072 1102 8 -0.08097467405374204 1102 9 0.19323805586786236 1102 10-11 0.2693240346362913 1102 12-13 0.3724032921884266 1102 14-15 0.5399254530458748 1102 16-17 0.39365470139587444 1102 18-19 0.5992965889611312 1102 20-21 1.0491308876675625 1102 22-23 0.8407333753791661 1102 24-25 0.9729042899149114 1102 26-27 0.40502897956403316 1102 28-29 0.997365481049588 1102 30-31 0.7731024482835664 1102 32-33 0.6222411649894042 1102 34-35 1.0478141196533848 1102 36-37 1.0664620661053696 1102 38-39 1.159465330362412 1102 40-41 0.9539635146651264 1102 42-43 0.7573520047606017 1102 44-45 0.8476001654720946 1102 46-47 0.49103533173610003 1102 48-49 0.1321322395111224 1102 50-51 0.6023851423689592 1102 52-53 0.4052728345842347 1102 54-55 0.2350695523292501 1102 56-57 0.2027024687180763 1102 58-59 0.4352305527886351 1102 60-61 0.3284616047453639 1102 62-63 0.30628534039258426 1102 64-65 0.38936113481131684 1102 66-67 0.3309066445740392 1102 68-69 0.2454419427531498 1102 70-71 0.2258731795678841 1102 72-73 0.2869171406054072 1102 74-75 -0.013159672427818236 1102 76-77 0.24226105350729554 1102 78-79 0.07666549457231397 1102 80-81 -0.25282792625073114 1102 82-83 -0.12441161182040972 1102 84-85 -0.4399729730710362 1102 86-87 0.023045282265439226 1102 88-89 0.23500695551127393 1102 90-91 -0.3121487408737984 1102 92-93 0.12077414740480208 1102 94-95 0.10937943521686222 1102 96-97 0.23099722658050226 1102 98-99 0.26230039176421727 1102 100-101 0.21537697940241074 1102 102-103 -0.03625762198614524 1102 104-105 0.32487798753152575 1102 106-107 0.2270524115414112 1102 108-109 -0.3231552611844073 1102 110-111 0.045333284449917244 1102 112-113 -0.32667327406753444 1102 114-115 -0.27460082178131273 1102 116-117 -0.3499307159879521 1102 118-119 -0.023889025923740093 1102 120-121 0.38608519381488904 1102 122-123 0.23761252441277136 1102 124-125 0.5714205564875492 1103 1 0.559213772688441 1103 2 0.577352110855152 1103 3 0.4353712728037422 1103 4 0.5950601430628346 1103 5 0.5810092888728704 1103 6 0.7307625515388878 1103 7 0.7753819666002073 1103 8 0.8894368043115328 1103 9 0.9647188095359489 1103 10-11 1.0788065529997297 1103 12-13 1.0232566452792682 1103 14-15 1.2887374921545884 1103 16-17 1.2718833625960713 1103 18-19 1.2295868962626457 1103 20-21 1.6336218336786068 1103 22-23 1.6416185289778227 1103 24-25 1.671486542926818 1103 26-27 1.4993917179879297 1103 28-29 1.4940273551453096 1103 30-31 1.390107085127525 1103 32-33 1.4446673740381542 1103 34-35 1.8416300337830087 1103 36-37 1.778124201850833 1103 38-39 1.8021523501433698 1103 40-41 1.486762159043181 1103 42-43 1.2858944799072312 1103 44-45 0.9106220195798258 1103 46-47 0.9036767755249322 1103 48-49 0.9397060762412082 1103 50-51 0.9279984071508451 1103 52-53 0.8714019787433038 1103 54-55 0.7242976729444948 1103 56-57 0.7535453952835276 1103 58-59 0.6702883000324498 1103 60-61 0.6185369338261602 1103 62-63 0.6337690259585713 1103 64-65 0.76625636891049 1103 66-67 0.7904022375319606 1103 68-69 0.893583748076427 1103 70-71 0.8705157253638873 1103 72-73 0.8367184604409275 1103 74-75 0.9430341611782538 1103 76-77 0.8660928939374131 1103 78-79 0.8409229590291893 1103 80-81 0.8260455220143257 1103 82-83 0.8807206302243138 1103 84-85 0.8804850615849666 1103 86-87 0.7749597257182721 1103 88-89 0.8094730657124636 1103 90-91 0.8200643368936369 1103 92-93 0.9239271294739417 1103 94-95 0.7967642037602296 1103 96-97 0.9240909124235266 1103 98-99 0.8731480114500343 1103 100-101 0.8315864062284462 1103 102-103 0.7922002254501876 1103 104-105 0.8216595575073633 1103 106-107 0.8603692723853413 1103 108-109 0.9744233578558514 1103 110-111 0.9762334606116241 1103 112-113 0.9793608347988076 1103 114-115 0.9970727949814489 1103 116-117 1.033400904585342 1103 118-119 1.0918333101009914 1103 120-121 1.0404075963452293 1103 122-123 1.0765796543376176 1103 124-125 1.1828295194634393 1104 1 0.8199470924811507 1104 2 0.7785072062274665 1104 3 0.7801981755286143 1104 4 0.7439757499381443 1104 5 0.7082610856779965 1104 6 0.6754070864125268 1104 7 0.8079575595072939 1104 8 0.9059471599191937 1104 9 1.1580598366754025 1104 10-11 1.1067215063023852 1104 12-13 1.0132897737809827 1104 14-15 1.12026151161745 1104 16-17 1.2259085282608524 1104 18-19 1.5401136324615976 1104 20-21 1.7429435879970576 1104 22-23 1.528889347983629 1104 24-25 1.926380691476961 1104 26-27 2.0153107477309753 1104 28-29 1.8687521256376733 1104 30-31 1.6909942929202728 1104 32-33 1.6829193983020048 1104 34-35 1.9899879436452217 1104 36-37 2.0108900093411783 1104 38-39 1.978885215909468 1104 40-41 1.6148384937151405 1104 42-43 1.6909265290193112 1104 44-45 1.4976424261311188 1104 46-47 1.4017955270913127 1104 48-49 1.474406262781116 1104 50-51 1.2225772729692075 1104 52-53 1.2739482395713502 1104 54-55 1.0989041856875303 1104 56-57 1.0859496666376245 1104 58-59 1.1688220672596294 1104 60-61 1.1670324101888632 1104 62-63 1.1777097601929505 1104 64-65 1.1628471679973273 1104 66-67 1.15244113623978 1104 68-69 1.214849891409866 1104 70-71 1.1652209035240517 1104 72-73 0.9949252438776597 1104 74-75 1.0942629208096157 1104 76-77 1.1100375849439423 1104 78-79 1.206814031541608 1104 80-81 1.2231352975369134 1104 82-83 1.1164823749336712 1104 84-85 1.118087336350353 1104 86-87 1.1555067787915796 1104 88-89 1.250311787085625 1104 90-91 1.2001533546164111 1104 92-93 1.1940768988676176 1104 94-95 1.2217527491646756 1104 96-97 1.0744871167135308 1104 98-99 1.139044325015245 1104 100-101 1.1209249244922574 1104 102-103 1.1193960706761104 1104 104-105 1.1887474608011814 1104 106-107 1.2611193219749062 1104 108-109 1.3091560745563093 1104 110-111 1.2757608898767145 1104 112-113 1.3354378503622115 1104 114-115 1.2655990257825636 1104 116-117 1.3492449709415233 1104 118-119 1.320715319303865 1104 120-121 1.2523469373206986 1104 122-123 1.2817872490239957 1104 124-125 1.6156019190995075 1105 1 0.23199131077730328 1105 2 0.4379196114897539 1105 3 0.5191045029263286 1105 4 0.6649421443586476 1105 5 0.6049712539791479 1105 6 0.6359843963382694 1105 7 0.7247412796208756 1105 8 0.5626036251066466 1105 9 0.4841193350974997 1105 10-11 0.7828853208571687 1105 12-13 0.6867896534329887 1105 14-15 0.7212789027751825 1105 16-17 0.7031998930885379 1105 18-19 0.8174061746066528 1105 20-21 1.335888433730691 1105 22-23 1.4067455780564515 1105 24-25 1.4676616534702482 1105 26-27 1.2421608365675603 1105 28-29 0.9974921431491772 1105 30-31 0.94020970537089 1105 32-33 0.9657360298138826 1105 34-35 1.5814506049522343 1105 36-37 1.5923944552403917 1105 38-39 1.6860001226659271 1105 40-41 1.3002130724971437 1105 42-43 0.9109059535814659 1105 44-45 1.01179915875856 1105 46-47 1.0693503900806398 1105 48-49 0.8300355403675539 1105 50-51 0.72067423392064 1105 52-53 0.7675491563091406 1105 54-55 0.661643936921827 1105 56-57 0.6433712136965966 1105 58-59 0.6065272983859238 1105 60-61 0.5224026052805222 1105 62-63 0.541035277689339 1105 64-65 0.593227232206182 1105 66-67 0.36475818693477535 1105 68-69 0.1691153267672938 1105 70-71 0.42345129668409953 1105 72-73 0.5460012683472542 1105 74-75 0.6592847782152589 1105 76-77 0.5615862514648775 1105 78-79 0.6907261350618121 1105 80-81 0.5432359712531465 1105 82-83 0.5691573417362292 1105 84-85 0.5443684465984227 1105 86-87 0.49189745513569605 1105 88-89 0.6534654424089581 1105 90-91 0.5000550413488298 1105 92-93 0.48185352484464516 1105 94-95 0.60441396024925 1105 96-97 0.7375799468798299 1105 98-99 0.5472573927147728 1105 100-101 0.5891622827199896 1105 102-103 0.5669975263636289 1105 104-105 0.6265255838607864 1105 106-107 0.6108282193546906 1105 108-109 0.5839267592957178 1105 110-111 0.6037991535632479 1105 112-113 0.6385940928767972 1105 114-115 0.6861985622974274 1105 116-117 0.6946196689087465 1105 118-119 0.6711334413867327 1105 120-121 0.5612219626112207 1105 122-123 0.7305535221662751 1105 124-125 0.7354892367461296 1106 1 0.19754103642447873 1106 2 0.5145696926398955 1106 3 0.24287824928091695 1106 4 0.3232366579364552 1106 5 0.1904872628229839 1106 6 0.19055799560038977 1106 7 -0.05735322153697098 1106 8 0.26024636948156044 1106 9 0.4256690704800441 1106 10-11 0.5593343446585095 1106 12-13 0.439223291084879 1106 14-15 0.7315394763738539 1106 16-17 0.612358106584729 1106 18-19 0.6343062539498519 1106 20-21 0.9655487404034382 1106 22-23 1.015907066174364 1106 24-25 1.1971719802553729 1106 26-27 1.4382503850813002 1106 28-29 1.2404456033991842 1106 30-31 0.9874012267852095 1106 32-33 0.9560141646778462 1106 34-35 1.151917034990607 1106 36-37 1.066156935863706 1106 38-39 1.2326598683753254 1106 40-41 0.9953391520713382 1106 42-43 0.9048643502499658 1106 44-45 0.8402745280717276 1106 46-47 0.5860554044852897 1106 48-49 0.7483935786567315 1106 50-51 0.6463686971105744 1106 52-53 0.6552969673646452 1106 54-55 0.44045658858512127 1106 56-57 0.5326989559820987 1106 58-59 0.5143834441059987 1106 60-61 0.5509725182331131 1106 62-63 0.5144751085064527 1106 64-65 0.4941378794322162 1106 66-67 0.3854301400504525 1106 68-69 0.4226679225761636 1106 70-71 0.5495990184102837 1106 72-73 0.4609056870067576 1106 74-75 0.5145065792601606 1106 76-77 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-2.2989453331963325 2216 80-81 -2.3056086539789504 2216 82-83 -2.067282211770216 2216 84-85 -2.1281675320546256 2216 86-87 -2.1685133390127156 2216 88-89 -2.2612544926384857 2216 90-91 -2.279961617487441 2216 92-93 -2.2508710203057554 2216 94-95 -2.3179926637982398 2216 96-97 -2.288441946183301 2216 98-99 -2.408106431716295 2216 100-101 -2.4008999452825392 2216 102-103 -2.249024062492353 2216 104-105 -2.474009171706438 2216 106-107 -2.450466093542534 2216 108-109 -2.501573922407882 2216 110-111 -2.559050473313704 2216 112-113 -2.555882760021003 2216 114-115 -2.6982806024415957 2216 116-117 -2.7385928325007924 2216 118-119 -2.7928401110964565 2216 120-121 -2.8446831042551075 2216 122-123 -2.827279252832664 2216 124-125 -2.8327496028786605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 268068.0 3 45387.0 4 27483.0 5 21832.0 6 20377.0 7 21919.0 8 22735.0 9 23950.0 10 25783.0 11 27871.0 12 29863.0 13 33572.0 14 37667.0 15 32981.0 16 24551.0 17 21424.0 18 19702.0 19 18189.0 20 17770.0 21 19136.0 22 22991.0 23 30395.0 24 41389.0 25 52173.0 26 62386.0 27 68875.0 28 78502.0 29 90477.0 30 101826.0 31 115299.0 32 139681.0 33 172179.0 34 219561.0 35 296100.0 36 437636.0 37 552748.0 38 175277.0 39 335.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.77256760706836 19.121923729530177 13.14756062128016 22.957948042121306 2 15.024087622420302 20.71316097600366 40.52150741177868 23.741243989797358 3 19.02674118163037 26.78957481003336 28.800671301761906 25.383012706574366 4 12.011702120811904 16.900869027883676 37.969133739944674 33.11829511135974 5 13.197064256476985 39.415689543630165 33.31307545848682 14.074170741406034 6 30.614043864622705 37.24130808627813 18.24931788832385 13.895330160775316 7 28.190690361205373 33.50519656601227 20.98062691166394 17.323486161118414 8 26.04477939434011 35.605586745814186 19.877972785971114 18.47166107387459 9 27.409648688319173 16.49865024155805 19.766859107570788 36.32484196255199 10-11 24.493006328095515 27.77875948263504 27.193944571383433 20.53428961788601 12-13 25.517659152385846 25.382066517191475 27.41102620175172 21.68924812867096 14-15 22.874536279219747 26.25440896851644 25.648301987495998 25.222752764767815 16-17 22.089702887225442 29.741405610860415 25.99478880953402 22.174102692380124 18-19 21.94921142984205 28.146022625036753 28.54025650290603 21.364509442215166 20-21 23.221155196547027 27.083118770861326 28.398406229957914 21.297319802633734 22-23 22.644328835223117 27.2369916220698 28.041475962162977 22.0772035805441 24-25 22.346423883117698 27.204918403327156 28.43296848211565 22.0156892314395 26-27 22.247494343765617 27.48172038479951 28.182034184372757 22.088751087062118 28-29 22.03329334488566 27.4122637318335 28.506097226490795 22.048345696790044 30-31 22.20765407497962 27.193178654815025 28.44566710907479 22.15350016113057 32-33 22.174992180612776 27.294796311180303 28.056166168342262 22.474045339864652 34-35 22.51456504412816 27.036121777579076 28.361371092044756 22.087942086248 36-37 22.43919980361125 27.033195609576115 28.093217946163385 22.434386640649254 38-39 22.229212805982286 27.181832543906097 28.321416346556116 22.267538303555494 40-41 22.480328428694378 27.109033281527406 28.156588897300484 22.254049392477732 42-43 22.347877506164835 27.213576563913566 27.914062143408046 22.524483786513553 44-45 22.475850490671213 27.25698881862173 27.724404811979475 22.542755878727583 46-47 22.48423321154472 27.552733456136203 27.30161758062742 22.66141575169166 48-49 22.493076672875848 27.52795631225855 27.219082353171842 22.75988466169376 50-51 22.615185666303876 27.615346581001532 27.090033236199368 22.67943451649522 52-53 22.828553373375186 27.579335242781788 26.984339206984014 22.607772176859005 54-55 22.650635866024462 27.426957301738742 27.097403968304945 22.825002863931854 56-57 22.562863249301113 27.7946186805776 26.896064436567695 22.746453633553593 58-59 22.71585882011504 27.33193118122782 27.041197701984064 22.91101229667307 60-61 22.79711211974349 27.47421363075619 27.132999282169855 22.595674967330464 62-63 22.67681082592511 27.46056023278677 27.185613269559955 22.677015671728164 64-65 22.49218802807072 27.36329158852744 27.106628186419883 23.037892196981954 66-67 22.483305073873705 27.711039357618183 26.837509740626874 22.96814582788124 68-69 22.652608588209528 27.405191294127736 27.0903754307558 22.85182468690694 70-71 22.36987716324046 27.440940095959863 27.264272014719754 22.924910726079922 72-73 22.684634943485218 27.50749775433114 26.89939696110531 22.90847034107833 74-75 22.528663668891106 27.73392450169539 26.789800736668724 22.94761109274478 76-77 22.622393289652333 27.5246932643004 27.00150429263941 22.851409153407857 78-79 22.60714061066893 27.596630247319016 26.873046220730796 22.923182921281253 80-81 22.467866549540698 27.80452446589316 26.87976570905705 22.847843275509092 82-83 22.695121778177747 27.8367695390207 26.711723697670465 22.756384985131092 84-85 22.57167014326715 27.600999388547404 26.67618465283243 23.15114581535302 86-87 22.669017822085763 27.525502546304832 26.77771750674538 23.027762124864022 88-89 22.76668128252632 27.43031095204842 26.780614776122047 23.02239298930321 90-91 22.604159854015773 27.6525518050434 26.943524020254845 22.79976432068598 92-93 22.607110914240756 27.603162616894615 26.909160488705297 22.880565980159332 94-95 22.519222356020713 27.41952947476695 26.941176298450948 23.12007187076139 96-97 22.76836999656474 27.347562634930426 26.828089639742714 23.05597772876212 98-99 22.361723077085113 27.376761580657096 26.752079652212025 23.50943569004577 100-101 22.283645705098525 27.463918702148533 26.989695864521135 23.26273972823181 102-103 22.66828843008815 27.328127697047183 26.88100664406145 23.12257722880322 104-105 22.497559645511174 27.333491610783174 26.97550956342486 23.193439180280794 106-107 22.31246758056914 27.46340133331812 27.03765174049008 23.186479345622658 108-109 22.37253846446407 27.557115231020834 26.60307364639316 23.467272658121935 110-111 22.19761929769077 27.641119966343553 26.81161603912387 23.34964469684181 112-113 22.369817646697424 27.56909560602559 26.89532750746762 23.165759239809365 114-115 22.17184158404255 27.811994417935164 26.7235356588036 23.292628339218687 116-117 22.068391996699912 27.792612248360925 26.842900567275873 23.296095187663283 118-119 22.181379074278325 27.859023606751137 26.522619357594447 23.43697796137609 120-121 22.028218403356146 27.860030540461427 26.789651584504032 23.322099471678396 122-123 22.23863327179799 27.88923053518193 26.620627309403787 23.25150888361629 124-125 22.241262862674454 27.828193530942407 26.50508361689451 23.42545998948863 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1897.0 1 1935.5 2 2170.5 3 2030.5 4 1459.5 5 1110.5 6 1001.0 7 1205.5 8 1624.0 9 1892.0 10 1828.5 11 1814.5 12 2003.5 13 2224.0 14 2479.0 15 2720.0 16 3009.5 17 3354.5 18 3741.5 19 4178.5 20 4817.0 21 5789.0 22 6938.0 23 8430.5 24 10288.5 25 13024.0 26 17334.5 27 22623.0 28 27420.5 29 32815.5 30 39336.5 31 45994.5 32 54097.0 33 62832.0 34 72784.5 35 81526.5 36 89745.0 37 98332.5 38 102146.0 39 102133.0 40 102247.0 41 104229.0 42 106522.0 43 110895.5 44 116579.5 45 119755.0 46 119987.0 47 119245.0 48 123237.5 49 125701.0 50 122607.0 51 118270.5 52 114214.0 53 112812.5 54 110695.5 55 104374.0 56 96313.5 57 88321.0 58 82249.0 59 75779.5 60 66185.0 61 54952.0 62 43579.5 63 33219.0 64 24848.0 65 19165.5 66 14547.0 67 11249.0 68 8577.0 69 6139.5 70 4370.0 71 3319.0 72 2743.0 73 2427.5 74 2031.5 75 1600.5 76 1158.5 77 770.5 78 551.5 79 377.5 80 264.5 81 189.0 82 144.5 83 115.5 84 84.5 85 66.0 86 54.5 87 37.5 88 22.5 89 22.5 90 24.5 91 18.0 92 13.5 93 12.5 94 6.0 95 1.0 96 4.5 97 6.5 98 4.0 99 2.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012784917892741268 2 0.004446927962692615 3 0.007401794569481787 4 0.007255514044393213 5 0.014306235353662424 6 8.19170940496008E-4 7 0.0074895628845349305 8 0.0 9 0.0020771834562577345 10-11 0.0 12-13 0.0038471778098294663 14-15 0.018255809531053893 16-17 0.02985585517057772 18-19 0.005704940478454342 20-21 0.00825022161499551 22-23 0.0018138785110983033 24-25 0.011570789534506113 26-27 0.018197297321018463 28-29 5.704940478454342E-4 30-31 0.0015944577234654443 32-33 0.008864599820367516 34-35 0.009303441395633234 36-37 0.011088063801713822 38-39 0.0 40-41 0.02483843316003967 42-43 0.009303441395633234 44-45 0.016895400647730166 46-47 0.004271391332586327 48-49 0.024531244057353668 50-51 0.0038032936523028946 52-53 0.0 54-55 0.017129449487871883 56-57 0.004534696277745759 58-59 3.218171551948603E-4 60-61 0.004403043805166043 62-63 0.025906280993186252 64-65 0.016939284805256735 66-67 0.0017699943535717316 68-69 0.020347621039820483 70-71 0.0028817263442448855 72-73 0.011702442007085829 74-75 0.0 76-77 0.00598287347612263 78-79 0.00102396367562001 80-81 0.003437592339581462 82-83 0.0015066894084123004 84-85 0.0 86-87 0.005734196583472057 88-89 0.01666135180758845 90-91 0.021488609135511354 92-93 0.004710232907852046 94-95 0.024721408739968812 96-97 0.017202589750416167 98-99 8.923112030402943E-4 100-101 0.00841113019259294 102-103 0.0 104-105 0.0012141283582351547 106-107 2.9256105017714572E-5 108-109 0.0 110-111 6.728904154074351E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 4.388415752657185E-5 122-123 0.0 124-125 0.024516616004844814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 3418090.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 45.79735157764514 #Duplication Level Percentage of deduplicated Percentage of total 1 74.74001619519817 34.228947986103826 2 10.424374141990413 9.548174551152956 3 4.826559643105789 6.631309466573679 4 2.7392442665094574 5.018005309215293 5 1.6821891597851455 3.8519904185391898 6 1.1411687629357319 3.1357504227356454 7 0.7991862757736284 2.562043039334361 8 0.6099279584150783 2.234646811885653 9 0.452693490945352 1.8658946665562222 >10 2.3687328631749587 18.982338851746658 >50 0.13742608589020147 4.335172923487649 >100 0.07534016020563969 6.2094583037181525 >500 0.002748371562086989 0.8148463548153837 >1k 3.271870902622018E-4 0.3673251842387786 >5k 6.543741805244035E-5 0.21409570989653534 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7144 0.2090056142465529 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4904 0.14347193900687227 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 5.8512210035429145E-5 0.0 0.0 0.0 5 0.0 5.8512210035429145E-5 0.0 0.0 2.9256105017714572E-5 6 0.0 5.8512210035429145E-5 0.0 0.0 2.9256105017714572E-5 7 0.0 5.8512210035429145E-5 0.0 0.0 2.9256105017714572E-5 8 0.0 5.8512210035429145E-5 0.0 0.0 2.9256105017714572E-5 9 0.0 5.8512210035429145E-5 0.0 0.0 2.9256105017714572E-5 10-11 0.0 8.776831505314372E-5 0.0 5.8512210035429145E-5 2.9256105017714572E-5 12-13 0.0 8.776831505314372E-5 0.0 2.04792735124002E-4 2.9256105017714572E-5 14-15 0.0 8.776831505314372E-5 2.9256105017714572E-5 2.3404884014171658E-4 2.9256105017714572E-5 16-17 0.0 8.776831505314372E-5 2.9256105017714572E-5 3.218171551948603E-4 2.9256105017714572E-5 18-19 0.0 8.776831505314372E-5 2.9256105017714572E-5 4.3884157526571856E-4 2.9256105017714572E-5 20-21 0.0 1.1702442007085829E-4 2.9256105017714572E-5 5.997501528631488E-4 2.9256105017714572E-5 22-23 0.0 1.1702442007085829E-4 2.9256105017714572E-5 9.21567308058009E-4 2.9256105017714572E-5 24-25 0.0 1.3165247257971557E-4 2.9256105017714572E-5 0.0015798296709565868 2.9256105017714572E-5 26-27 0.0 1.6090857759743014E-4 2.9256105017714572E-5 0.0024282567164703095 2.9256105017714572E-5 28-29 0.0 1.7553663010628744E-4 2.9256105017714572E-5 0.0036131289696877495 2.9256105017714572E-5 30-31 0.0 2.3404884014171658E-4 2.9256105017714572E-5 0.00684592857414521 2.9256105017714572E-5 32-33 0.0 2.6330494515943115E-4 2.9256105017714572E-5 0.013677229095781562 2.9256105017714572E-5 34-35 0.0 2.6330494515943115E-4 2.9256105017714572E-5 0.023258603489083086 2.9256105017714572E-5 36-37 0.0 2.779329976682884E-4 2.9256105017714572E-5 0.03853029030833009 2.9256105017714572E-5 38-39 2.9256105017714572E-5 3.218171551948603E-4 2.9256105017714572E-5 0.06540202276710093 2.9256105017714572E-5 40-41 2.9256105017714572E-5 3.364452077037176E-4 2.9256105017714572E-5 0.09717415281633895 2.9256105017714572E-5 42-43 2.9256105017714572E-5 3.6570131272143215E-4 2.9256105017714572E-5 0.1289755389705947 2.9256105017714572E-5 44-45 2.9256105017714572E-5 3.803293652302894E-4 2.9256105017714572E-5 0.1675643414889602 2.9256105017714572E-5 46-47 2.9256105017714572E-5 3.803293652302894E-4 2.9256105017714572E-5 0.21570526229560955 2.9256105017714572E-5 48-49 2.9256105017714572E-5 3.949574177391467E-4 2.9256105017714572E-5 0.26941947110813347 2.9256105017714572E-5 50-51 2.9256105017714572E-5 4.09585470248004E-4 2.9256105017714572E-5 0.3289263887141649 2.9256105017714572E-5 52-53 2.9256105017714572E-5 4.09585470248004E-4 2.9256105017714572E-5 0.3865316594940449 2.9256105017714572E-5 54-55 2.9256105017714572E-5 4.6809768028343316E-4 2.9256105017714572E-5 0.44887641928679467 2.9256105017714572E-5 56-57 2.9256105017714572E-5 4.6809768028343316E-4 2.9256105017714572E-5 0.522645688088962 2.9256105017714572E-5 58-59 2.9256105017714572E-5 4.827257327922904E-4 2.9256105017714572E-5 0.6082783074758125 4.388415752657186E-5 60-61 2.9256105017714572E-5 5.119818378100051E-4 2.9256105017714572E-5 0.7052476675570275 5.8512210035429145E-5 62-63 2.9256105017714572E-5 5.266098903188623E-4 2.9256105017714572E-5 0.8022462837432601 5.8512210035429145E-5 64-65 2.9256105017714572E-5 5.704940478454342E-4 2.9256105017714572E-5 0.9026093520065299 5.8512210035429145E-5 66-67 2.9256105017714572E-5 5.851221003542915E-4 2.9256105017714572E-5 1.0189901377669985 5.8512210035429145E-5 68-69 2.9256105017714572E-5 5.851221003542915E-4 2.9256105017714572E-5 1.1595510943246081 5.8512210035429145E-5 70-71 2.9256105017714572E-5 5.851221003542915E-4 2.9256105017714572E-5 1.3146962192335485 5.8512210035429145E-5 72-73 2.9256105017714572E-5 5.851221003542915E-4 2.9256105017714572E-5 1.474141991580093 5.8512210035429145E-5 74-75 2.9256105017714572E-5 5.851221003542915E-4 2.9256105017714572E-5 1.6382687407294716 5.8512210035429145E-5 76-77 2.9256105017714572E-5 5.851221003542915E-4 2.9256105017714572E-5 1.8202124578346388 5.8512210035429145E-5 78-79 2.9256105017714572E-5 5.851221003542915E-4 2.9256105017714572E-5 2.033562603676322 5.8512210035429145E-5 80-81 2.9256105017714572E-5 6.290062578808632E-4 2.9256105017714572E-5 2.2747353053898522 5.8512210035429145E-5 82-83 2.9256105017714572E-5 6.436343103897206E-4 2.9256105017714572E-5 2.5290732543613537 5.8512210035429145E-5 84-85 2.9256105017714572E-5 6.436343103897206E-4 2.9256105017714572E-5 2.7866878870948395 5.8512210035429145E-5 86-87 2.9256105017714572E-5 6.436343103897206E-4 2.9256105017714572E-5 3.060013048222838 5.8512210035429145E-5 88-89 2.9256105017714572E-5 6.436343103897206E-4 2.9256105017714572E-5 3.3739017989578977 5.8512210035429145E-5 90-91 2.9256105017714572E-5 6.436343103897206E-4 2.9256105017714572E-5 3.725033571380508 5.8512210035429145E-5 92-93 2.9256105017714572E-5 6.436343103897206E-4 2.9256105017714572E-5 4.0984292397215984 5.8512210035429145E-5 94-95 2.9256105017714572E-5 6.436343103897206E-4 2.9256105017714572E-5 4.480455459042916 5.8512210035429145E-5 96-97 2.9256105017714572E-5 6.436343103897206E-4 2.9256105017714572E-5 4.868830253153076 5.8512210035429145E-5 98-99 4.388415752657186E-5 6.436343103897206E-4 2.9256105017714572E-5 5.287602140376643 5.8512210035429145E-5 100-101 5.8512210035429145E-5 6.728904154074351E-4 2.9256105017714572E-5 5.769742751068579 5.8512210035429145E-5 102-103 5.8512210035429145E-5 6.728904154074351E-4 2.9256105017714572E-5 6.28665424257407 7.314026254428644E-5 104-105 5.8512210035429145E-5 6.875184679162924E-4 2.9256105017714572E-5 6.806008618848538 8.776831505314372E-5 106-107 5.8512210035429145E-5 7.021465204251498E-4 2.9256105017714572E-5 7.327352410264211 8.776831505314372E-5 108-109 5.8512210035429145E-5 7.021465204251498E-4 2.9256105017714572E-5 7.8722473662191454 8.776831505314372E-5 110-111 5.8512210035429145E-5 7.314026254428643E-4 2.9256105017714572E-5 8.48383161356196 8.776831505314372E-5 112-113 5.8512210035429145E-5 7.314026254428643E-4 2.9256105017714572E-5 9.135438212569008 8.776831505314372E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 7525 0.0 50.21012 1 GTATCAA 13420 0.0 48.150566 1 TCAACGC 15995 0.0 39.72757 4 ATCAACG 16020 0.0 39.554153 3 GTACATG 20345 0.0 39.014153 1 CAACGCA 16440 0.0 38.652218 5 AACGCAG 16795 0.0 37.546864 6 TACATGG 20745 0.0 37.28507 2 TATCAAC 17500 0.0 36.344997 2 ACATGGG 21790 0.0 34.814323 3 ACGCAGA 19345 0.0 32.228992 7 CGCAGAG 18945 0.0 31.969273 8 CATGGGA 15225 0.0 30.48192 4 GCAGAGT 19975 0.0 28.474562 9 GAGTACT 7550 0.0 26.562212 12-13 CATGGGG 7695 0.0 26.211754 4 ATGGGAT 6165 0.0 25.092579 5 GTATAGG 1055 0.0 24.25158 1 ATGGGAG 6115 0.0 23.546366 5 CAGAGTA 19470 0.0 23.486994 10-11 >>END_MODULE