FastQCFastQC Report
Thu 26 May 2016
SRR1364533_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364533_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3418090
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122490.35835803036198577No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102120.2987633444409012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63640.18618585233273552No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98200.057.611941
GTATCAA176900.049.7749061
ATCAACG225950.038.8522873
TCAACGC227700.038.422914
CAACGCA232750.037.5012785
TATCAAC235850.036.8679052
AACGCAG237700.036.6952936
GTACATG197300.032.5119481
ACGCAGA275250.031.6028067
CGCAGAG275600.031.5626728
TACATGG201800.031.4311262
GCAGAGT289150.029.2802359
ACATGGG210700.029.0010873
GAGTACT155000.028.07901612-13
AGTACTT164650.026.84888812-13
GTACTTT175750.024.84848814-15
CATGGGA148850.024.5669364
CAGAGTA285100.024.1861310-11
AGAGTAC284800.023.89825610-11
CATGGGG74300.022.4440214
Produced by FastQC (version 0.11.5)