FastQCFastQC Report
Thu 26 May 2016
SRR1364532_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364532_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526956
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33880.2218793468836037No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33870.2218138571117963No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23320.152722147854948No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61450.041.828991
GTACATG87050.036.5679471
GGTATCA39950.034.999881
TACATGG90150.034.3182142
ATCAACG74900.033.9182243
TCAACGC75700.033.2454034
TATCAAC77650.033.1767272
ACATGGG89850.033.0422443
CAACGCA76000.032.8793145
AACGCAG76800.032.4551056
CATGGGA59500.029.197994
ACGCAGA88050.028.1056847
CGCAGAG87300.027.6002888
ATGGGAG25250.025.2121265
GCAGAGT93300.024.1036629
GAGTACT34800.022.91459712-13
ATGGGAT22400.022.3109655
CATGGGG34200.022.2674734
AGTACTT39450.021.64669212-13
GTATAGG4700.021.5212061
Produced by FastQC (version 0.11.5)