FastQCFastQC Report
Thu 26 May 2016
SRR1364532_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364532_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1526956
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76940.5038783042864365No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64990.42561802697654677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46130.30210431734771664No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16590.10864753142854149No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16040.10504559397913234No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51250.047.5841941
GTATCAA82650.045.7382661
ATCAACG110750.034.255513
CAACGCA112300.033.9073525
TCAACGC111950.033.8351254
TATCAAC111750.033.68252
AACGCAG113550.033.4816936
GTACATG90200.030.8043941
ACGCAGA132300.028.601647
TACATGG95400.028.4676252
CGCAGAG132450.028.2548078
ACATGGG92400.028.1672363
AGTACTT82300.027.21816312-13
GAGTACT79300.026.33465212-13
CATGGGG35550.025.9673024
GCAGAGT141200.025.7875679
GTACTTT84400.024.88432914-15
AGAGTAC133000.024.1334410-11
TACTTTT94100.023.86827314-15
CAGAGTA135300.022.1841510-11
Produced by FastQC (version 0.11.5)