##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364532_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1526956 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.08110384320177 31.0 30.0 34.0 26.0 34.0 2 29.98360005134398 31.0 30.0 34.0 26.0 34.0 3 30.225636495092196 31.0 30.0 34.0 26.0 34.0 4 33.79542763511195 35.0 35.0 37.0 28.0 37.0 5 33.21468922483687 35.0 33.0 37.0 27.0 37.0 6 33.23861853255759 36.0 33.0 37.0 27.0 37.0 7 33.03374622451466 36.0 33.0 37.0 26.0 37.0 8 32.95382447169401 36.0 33.0 37.0 26.0 37.0 9 34.18431310397942 38.0 34.0 39.0 25.0 39.0 10-11 34.11573810902213 38.0 34.0 39.0 25.0 39.0 12-13 33.999271426288644 38.0 34.0 39.0 25.0 39.0 14-15 34.952285789505396 39.0 34.0 41.0 24.5 41.0 16-17 34.96688378709013 39.0 34.0 41.0 25.0 41.0 18-19 35.00669436447416 39.0 34.0 41.0 25.0 41.0 20-21 35.02636258019223 39.0 34.0 41.0 25.0 41.0 22-23 34.983592520020224 39.0 34.0 41.0 25.0 41.0 24-25 34.9316689544427 39.0 34.0 41.0 24.0 41.0 26-27 34.8051836464181 39.0 34.0 41.0 24.0 41.0 28-29 34.64542396768473 39.0 33.5 41.0 24.0 41.0 30-31 34.467796059611416 38.5 33.0 41.0 23.5 41.0 32-33 34.22765619965474 38.0 33.0 40.0 22.5 41.0 34-35 34.026064274281644 38.0 33.0 40.0 20.5 41.0 36-37 33.81042348306042 38.0 33.0 40.0 18.5 41.0 38-39 33.635620803742874 38.0 33.0 40.0 17.0 41.0 40-41 33.64883369265388 38.0 33.0 40.0 16.5 41.0 42-43 33.49338193111 38.0 33.0 40.0 15.0 41.0 44-45 33.29842346472328 38.0 32.0 40.0 13.5 41.0 46-47 33.01371683270507 38.0 32.0 40.0 8.5 41.0 48-49 32.6925173351426 38.0 31.0 40.0 6.0 41.0 50-51 32.52073668134511 38.0 31.0 40.0 2.0 41.0 52-53 32.33348472385583 37.5 31.0 40.0 2.0 41.0 54-55 32.13266328564805 37.0 31.0 40.0 2.0 41.0 56-57 31.921659170270786 37.0 31.0 40.0 2.0 41.0 58-59 31.716294379143864 36.5 30.0 40.0 2.0 41.0 60-61 31.480486667592253 36.0 30.0 40.0 2.0 41.0 62-63 31.291085663241113 36.0 30.0 39.5 2.0 41.0 64-65 31.146208535150983 35.5 30.0 39.0 2.0 41.0 66-67 30.838988811727383 35.0 30.0 39.0 2.0 41.0 68-69 30.55717453548105 35.0 30.0 39.0 2.0 40.5 70-71 30.24186453309722 35.0 29.0 38.0 2.0 40.0 72-73 29.949266383576216 35.0 29.0 37.0 2.0 40.0 74-75 29.608125905396093 35.0 29.0 37.0 2.0 39.0 76-77 29.294614252146097 35.0 29.0 36.0 2.0 39.0 78-79 29.01613438763134 34.0 29.0 36.0 2.0 38.5 80-81 28.745639036095344 34.0 29.0 35.5 2.0 37.0 82-83 28.46777510288443 34.0 28.5 35.0 2.0 37.0 84-85 28.244649158194473 34.0 28.0 35.0 2.0 36.5 86-87 28.031560503380582 34.0 28.0 35.0 2.0 36.0 88-89 27.85331535420798 34.0 27.0 35.0 2.0 36.0 90-91 27.70303106310856 34.0 27.0 35.0 2.0 35.5 92-93 27.594446074412097 34.0 27.0 35.0 2.0 35.0 94-95 27.483571890742105 34.0 27.0 35.0 2.0 35.0 96-97 27.3642531284464 34.0 27.0 35.0 2.0 35.0 98-99 27.273720067899795 34.0 26.5 35.0 2.0 35.0 100-101 27.171747254013866 34.0 26.0 35.0 2.0 35.0 102-103 25.90837064067334 31.5 24.5 34.0 2.0 35.0 104-105 26.832698191696423 33.5 25.0 35.0 2.0 35.0 106-107 26.99668065091594 34.0 25.0 35.0 2.0 35.0 108-109 26.992705749216086 34.0 25.0 35.0 2.0 35.0 110-111 26.910390345235882 34.0 25.0 35.0 2.0 35.0 112-113 26.823016511281267 34.0 25.0 35.0 2.0 35.0 114-115 26.713668239294385 34.0 25.0 35.0 2.0 35.0 116-117 26.587718310154322 34.0 25.0 35.0 2.0 35.0 118-119 26.453843136278977 34.0 25.0 35.0 2.0 35.0 120-121 26.257983858081044 34.0 25.0 35.0 2.0 35.0 122-123 25.963108301745432 34.0 24.0 35.0 2.0 35.0 124-125 24.198564005773576 31.5 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.6513530221081609 1101 2 0.8775534414764685 1101 3 0.716496905754525 1101 4 0.6340407572809426 1101 5 0.8845204263173798 1101 6 0.828002163903129 1101 7 0.8555701014768005 1101 8 0.7529117382595416 1101 9 1.0229060522601543 1101 10-11 0.9775555070168949 1101 12-13 0.9607368129655498 1101 14-15 1.0654246538673249 1101 16-17 1.1030944772926787 1101 18-19 1.1166519327160884 1101 20-21 1.134315109103433 1101 22-23 1.0360335026142025 1101 24-25 1.0983202242087629 1101 26-27 1.0684710422994073 1101 28-29 1.2366244212760407 1101 30-31 1.328087463200788 1101 32-33 1.3567870233090389 1101 34-35 1.4204229945592743 1101 36-37 1.3502541541442383 1101 38-39 1.32283005323346 1101 40-41 1.1087482924619039 1101 42-43 1.0992616495264471 1101 44-45 1.0389373239121156 1101 46-47 1.135127632737266 1101 48-49 1.2904139166807127 1101 50-51 1.3625143485698814 1101 52-53 1.3546692337868507 1101 54-55 1.354852899896514 1101 56-57 1.312861241194561 1101 58-59 1.2061488880438205 1101 60-61 1.1498035121735697 1101 62-63 1.2416248826499796 1101 64-65 1.2143194272167186 1101 66-67 1.3189093732341277 1101 68-69 1.3292614507497476 1101 70-71 1.3066964328896944 1101 72-73 1.2033184028812194 1101 74-75 1.289870361953536 1101 76-77 1.350796118001476 1101 78-79 1.284513355475685 1101 80-81 1.1068745865909264 1101 82-83 1.2612759227520343 1101 84-85 1.2880216523690606 1101 86-87 1.378487312786465 1101 88-89 1.3560274936539471 1101 90-91 1.203829922830291 1101 92-93 1.1868943325077481 1101 94-95 1.1147823380934376 1101 96-97 1.2602057258921207 1101 98-99 1.1750616630401005 1101 100-101 1.11973338581992 1101 102-103 1.0671719137510465 1101 104-105 1.2339165410564377 1101 106-107 1.1053877335290707 1101 108-109 1.086931951433879 1101 110-111 1.088319643024576 1101 112-113 1.203825349125534 1101 114-115 1.2115052207356278 1101 116-117 1.154244328139196 1101 118-119 1.2353385019492222 1101 120-121 1.133816311405969 1101 122-123 1.0493912340942266 1101 124-125 1.3449320826551734 1102 1 0.5030220566164658 1102 2 -0.23247858599883386 1102 3 0.2981229854542917 1102 4 0.4046835400275839 1102 5 0.5790889502136238 1102 6 0.4737512530708514 1102 7 0.4402307721390315 1102 8 0.5555095771348348 1102 9 0.7692067025540226 1102 10-11 0.9496012311953805 1102 12-13 1.0033168238322219 1102 14-15 1.2696699531613689 1102 16-17 1.1549163693441216 1102 18-19 1.1745968471268569 1102 20-21 0.9596805320939907 1102 22-23 1.2146773909855426 1102 24-25 1.1816846484971677 1102 26-27 1.1626734133259689 1102 28-29 0.8743719498294737 1102 30-31 1.052268984329558 1102 32-33 1.088125341053285 1102 34-35 1.1988023064861082 1102 36-37 0.8069188605641102 1102 38-39 0.20898966813028608 1102 40-41 -0.11291865288588099 1102 42-43 0.2454580503313295 1102 44-45 -0.172783075128919 1102 46-47 -0.05933226239521616 1102 48-49 -0.32531192631071804 1102 50-51 -0.22646180353010692 1102 52-53 -0.5363668267919763 1102 54-55 -0.46369715824736346 1102 56-57 -0.46736769819058566 1102 58-59 -0.2699917424124969 1102 60-61 -0.49254415067762736 1102 62-63 -0.4255991462600548 1102 64-65 -0.38007394464664657 1102 66-67 -0.5024718576112832 1102 68-69 -0.1451383705910061 1102 70-71 -0.6365893047413209 1102 72-73 -0.5303766661312217 1102 74-75 -0.3404267121221807 1102 76-77 -0.5785142353244765 1102 78-79 -0.5083277056661935 1102 80-81 -0.7075711811149006 1102 82-83 -0.7559862873674668 1102 84-85 -0.7874641532828974 1102 86-87 -0.48570739545715114 1102 88-89 -0.6780335007333775 1102 90-91 -0.5879530979441547 1102 92-93 -0.4751803517579134 1102 94-95 -0.26493765752349674 1102 96-97 -0.5472326334457662 1102 98-99 -0.6474042440214518 1102 100-101 -0.457984902069434 1102 102-103 -0.6208408737312823 1102 104-105 -0.48855981180770414 1102 106-107 -0.4460080797325894 1102 108-109 -0.3426383146933212 1102 110-111 -0.36362217669869423 1102 112-113 -0.21768894721600773 1102 114-115 -0.3000826057146604 1102 116-117 -0.35751592040644553 1102 118-119 -0.22559997852963676 1102 120-121 0.04055339908587641 1102 122-123 9.95887576056731E-4 1102 124-125 0.3045726105492612 1103 1 0.43913563163657443 1103 2 -0.05338902455220307 1103 3 0.35828710369728967 1103 4 0.42967649749104453 1103 5 0.5649151192699264 1103 6 0.5422424208062608 1103 7 0.7585753275011768 1103 8 0.7015661661777344 1103 9 1.0045257042223241 1103 10-11 1.0869201227734635 1103 12-13 0.9930314825363311 1103 14-15 1.0359826867340658 1103 16-17 0.9967275707008625 1103 18-19 1.0546820203923062 1103 20-21 0.9235370346265412 1103 22-23 1.113254960501898 1103 24-25 1.1157306985912712 1103 26-27 1.2151081691532823 1103 28-29 1.2354291321787016 1103 30-31 1.27134167797265 1103 32-33 1.2358009992471324 1103 34-35 1.274578571147579 1103 36-37 1.1666564839370608 1103 38-39 1.136628676563987 1103 40-41 1.127384502837124 1103 42-43 1.2700809294040667 1103 44-45 1.1400095425023977 1103 46-47 1.116108893712152 1103 48-49 1.0181561519996194 1103 50-51 1.0438080832312409 1103 52-53 1.1129778030429662 1103 54-55 1.05171556867802 1103 56-57 1.1857987576602795 1103 58-59 1.0461137720221743 1103 60-61 1.1417494500517904 1103 62-63 1.0940058660070022 1103 64-65 1.072468787217563 1103 66-67 1.066617092434612 1103 68-69 1.1666851483002283 1103 70-71 1.1423229504912484 1103 72-73 1.0584031970331012 1103 74-75 1.205566432189915 1103 76-77 1.3167732934908791 1103 78-79 1.1340859063617508 1103 80-81 1.1155930595987158 1103 82-83 1.1399754012337588 1103 84-85 1.128074263158716 1103 86-87 1.262761102834073 1103 88-89 1.2661373265375317 1103 90-91 1.2880101677281317 1103 92-93 1.204690316986877 1103 94-95 1.1686060344874747 1103 96-97 1.1954716510639471 1103 98-99 1.1983077321381117 1103 100-101 1.3026417094048242 1103 102-103 1.0549867409734972 1103 104-105 1.090322025761978 1103 106-107 1.2750831886839542 1103 108-109 1.1261329164830087 1103 110-111 1.1356336667019598 1103 112-113 1.0940555276545645 1103 114-115 1.1867183761073115 1103 116-117 1.315870091163692 1103 118-119 1.1913512307391194 1103 120-121 1.1063487847816553 1103 122-123 1.1456549211933797 1103 124-125 1.469858133741635 1104 1 0.3358797843062753 1104 2 0.59417252098185 1104 3 0.2655959638322898 1104 4 -0.05511026276280262 1104 5 0.269627876877621 1104 6 0.4025295510733429 1104 7 0.6157995555190254 1104 8 0.28529975665157536 1104 9 0.344221172120875 1104 10-11 0.5305875145165544 1104 12-13 0.40666886957016857 1104 14-15 0.4983320240740099 1104 16-17 0.521178044990549 1104 18-19 0.36691174254600867 1104 20-21 0.2957824543112437 1104 22-23 0.337887705551573 1104 24-25 0.5504097737824409 1104 26-27 0.3787227274920326 1104 28-29 0.47653603236482667 1104 30-31 0.5654118683612737 1104 32-33 0.6779055448036075 1104 34-35 0.6920037275622661 1104 36-37 0.6712250036598846 1104 38-39 0.708753449510418 1104 40-41 0.6125543725476206 1104 42-43 0.6063614218580398 1104 44-45 0.5448442923510228 1104 46-47 0.6385660506650339 1104 48-49 0.6723206172744369 1104 50-51 0.6147654980721384 1104 52-53 0.7306978039399681 1104 54-55 0.5015850100701655 1104 56-57 0.6531738165260208 1104 58-59 0.7396126619824841 1104 60-61 0.7559412738806373 1104 62-63 0.6905569838954619 1104 64-65 0.7175806209134343 1104 66-67 0.8151853430569034 1104 68-69 0.4508167384601194 1104 70-71 0.13340791898406223 1104 72-73 0.1699010554413718 1104 74-75 0.5693973473559737 1104 76-77 0.670584698868403 1104 78-79 0.5648159312535519 1104 80-81 0.5771575203885249 1104 82-83 0.5095733201896486 1104 84-85 0.5697533607749357 1104 86-87 0.5812187979880186 1104 88-89 0.5950333416071629 1104 90-91 0.635821976756457 1104 92-93 0.6742611464892683 1104 94-95 0.5303608390532126 1104 96-97 0.5354143326507455 1104 98-99 0.5511575516165017 1104 100-101 0.6127722680126766 1104 102-103 0.5624131083405715 1104 104-105 0.6610850603189711 1104 106-107 0.6296296870132885 1104 108-109 0.6389289158551108 1104 110-111 0.5935719978301641 1104 112-113 0.6678140996277477 1104 114-115 0.7168055198294176 1104 116-117 0.5818919331568289 1104 118-119 0.5745173778922599 1104 120-121 0.5635168604886012 1104 122-123 0.5739721010198124 1104 124-125 0.974389517591117 1105 1 0.23116699979614808 1105 2 -0.08918355825617752 1105 3 0.18027717833839318 1105 4 0.16360082251198094 1105 5 -0.13106058173705293 1105 6 0.22524814875701793 1105 7 0.4925289746488417 1105 8 0.5459127334436005 1105 9 0.6187755700102429 1105 10-11 0.5318234846457841 1105 12-13 0.5373059933907669 1105 14-15 0.691152575878327 1105 16-17 0.5986891123786506 1105 18-19 0.5095509233357021 1105 20-21 0.5962837287377525 1105 22-23 0.5786864355967296 1105 24-25 0.6010602231854776 1105 26-27 0.6510241181820078 1105 28-29 0.6549946470408585 1105 30-31 0.7590664764192283 1105 32-33 0.9216546840067608 1105 34-35 0.9157621678430914 1105 36-37 0.8708143253072507 1105 38-39 0.8371168175737438 1105 40-41 0.6422292819962578 1105 42-43 0.6362837936328489 1105 44-45 0.7834868716114087 1105 46-47 0.6660595890990564 1105 48-49 0.6388167939949128 1105 50-51 0.467982240011942 1105 52-53 0.5834095152488317 1105 54-55 0.5766667075168428 1105 56-57 0.5501802110169152 1105 58-59 0.21418512542656742 1105 60-61 0.31111070553113507 1105 62-63 0.4907039230360084 1105 64-65 0.2602492253046158 1105 66-67 0.44478718538574924 1105 68-69 0.5549405143399184 1105 70-71 0.6106031968984738 1105 72-73 0.46216354258531567 1105 74-75 0.45653270486614517 1105 76-77 0.438194500773335 1105 78-79 0.4233790041865184 1105 80-81 0.4786151065800013 1105 82-83 0.359473601904984 1105 84-85 0.5054625232307757 1105 86-87 0.5371172016011307 1105 88-89 0.4745727593954747 1105 90-91 0.2695897181149469 1105 92-93 0.42428788708757637 1105 94-95 0.3501765167351003 1105 96-97 0.3690447042466829 1105 98-99 0.34901061247595777 1105 100-101 0.3125248951199531 1105 102-103 0.08229532450119592 1105 104-105 0.2823422261732844 1105 106-107 0.20674715783563258 1105 108-109 0.27937107477833223 1105 110-111 0.1850286896872504 1105 112-113 0.11727946652843357 1105 114-115 0.09017599785115138 1105 116-117 -0.08846275986288887 1105 118-119 0.1871052238878761 1105 120-121 0.3053477418551438 1105 122-123 0.1873661894694827 1105 124-125 0.628021051821765 1106 1 -0.04869503157301125 1106 2 -0.6418348438466062 1106 3 0.07233550038698766 1106 4 -0.11529995200297094 1106 5 0.18576960491785854 1106 6 0.4237400773891338 1106 7 0.48308893938826003 1106 8 0.5556144683454818 1106 9 0.69968461742355 1106 10-11 0.5342380444249528 1106 12-13 0.626309968905538 1106 14-15 0.618911151072389 1106 16-17 0.5145510466072167 1106 18-19 0.6782324718436499 1106 20-21 0.6392892802498054 1106 22-23 0.5094411454653525 1106 24-25 0.6003786079839912 1106 26-27 0.7477179672980299 1106 28-29 0.7055673777932441 1106 30-31 0.7361873309084288 1106 32-33 0.5880987368280444 1106 34-35 0.5598638856366378 1106 36-37 0.6204739707876001 1106 38-39 0.48076889768717024 1106 40-41 0.4659794668533408 1106 42-43 0.5785813787468541 1106 44-45 0.32843976567597366 1106 46-47 0.3109375254270219 1106 48-49 0.548409354475794 1106 50-51 0.568337709316971 1106 52-53 0.5215170063829362 1106 54-55 0.40979059656199723 1106 56-57 0.052098012681938144 1106 58-59 0.4804494681617584 1106 60-61 0.5311004260272156 1106 62-63 0.4849748031877219 1106 64-65 0.6443175528336731 1106 66-67 0.46229985715785915 1106 68-69 0.5273714357836319 1106 70-71 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-0.7685988334752345 2216 76-77 -0.9847522247696752 2216 78-79 -1.0775458598937 2216 80-81 -0.8083651693361951 2216 82-83 -0.6749702033154072 2216 84-85 -0.7915036177905179 2216 86-87 -0.978259735279515 2216 88-89 -0.9500036700005339 2216 90-91 -0.8758034872296427 2216 92-93 -0.8620580330331222 2216 94-95 -0.8211328850654809 2216 96-97 -0.8567592893373686 2216 98-99 -0.872451949102274 2216 100-101 -0.9818733431616096 2216 102-103 -0.864141615198811 2216 104-105 -0.7860015146590378 2216 106-107 -0.9365515304004042 2216 108-109 -0.9393788061608959 2216 110-111 -0.9097484475964919 2216 112-113 -0.9955472838554194 2216 114-115 -1.1246286853328975 2216 116-117 -1.2038288384010905 2216 118-119 -1.309055304322861 2216 120-121 -1.3162523587273967 2216 122-123 -1.300011463680768 2216 124-125 -1.626907111670583 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46537.0 3 16326.0 4 8138.0 5 6463.0 6 7194.0 7 8438.0 8 9414.0 9 10058.0 10 11003.0 11 12130.0 12 13460.0 13 15498.0 14 18136.0 15 15809.0 16 10472.0 17 9324.0 18 8660.0 19 8165.0 20 8062.0 21 8280.0 22 9383.0 23 11451.0 24 15021.0 25 19275.0 26 24268.0 27 27013.0 28 30748.0 29 36411.0 30 41988.0 31 49160.0 32 59187.0 33 74285.0 34 96459.0 35 134995.0 36 212523.0 37 318562.0 38 114322.0 39 338.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.81473984233345 19.534604437370618 14.256000335628102 23.394655384667825 2 15.438830875828035 20.850516223464346 39.455124741724546 24.255528158983076 3 18.83047055710839 26.098787391385216 29.516371133156422 25.55437091834997 4 12.608768335655165 17.201212816520528 36.64410390887486 33.54591493894944 5 12.840317599197357 39.207154626590416 33.59730077356519 14.355227000647039 6 31.097818142762463 37.56964837231721 16.985230746661987 14.347302738258339 7 27.65617171766786 33.559670579759974 20.860946018959677 17.92321168361249 8 26.399689823783756 35.66175074040163 19.02914058396436 18.90941885185025 9 26.726225210409844 16.01898223918905 19.465636606433552 37.78915594396755 10-11 24.935271186468427 27.291573547879022 26.678815086962715 21.094340178689837 12-13 26.064045067441366 24.577852930929247 27.687863959406823 21.670238042222564 14-15 22.886627307288034 25.466107383649156 26.083938295496594 25.563327013566216 16-17 21.827117626198344 28.966702609742352 26.61931305611213 22.586866707947177 18-19 21.870310233910757 26.68428809862413 29.86426672892673 21.58113493853838 20-21 22.663861487454465 25.938422496009167 29.983119806802833 21.414596209733535 22-23 22.376610720937602 25.739379523705985 29.990975509444933 21.893034245911476 24-25 22.019724209472965 26.085427477936495 29.98907630606252 21.90577200652802 26-27 21.904036527575123 26.276755846271932 29.870900012835992 21.948307613316953 28-29 21.837302450103344 26.011456780679993 30.1782107670424 21.97303000217426 30-31 21.903152415655722 26.017383605028567 30.116421167342082 21.963042811973626 32-33 21.677572896664998 26.194205988908653 29.820636612973782 22.307584501452563 34-35 21.93684690325065 26.092729587493025 29.902269613531757 22.068153895724567 36-37 22.233810273511484 25.887189938675377 29.668241913977877 22.210757873835266 38-39 22.04847421929643 25.974520549380596 29.771388304574593 22.20561692674838 40-41 21.988059904804068 25.937355103879877 29.960686489983996 22.113898501332063 42-43 22.09867212938683 25.98352539300412 29.499049088513356 22.41875338909569 44-45 22.133283473787063 26.136051071543648 29.303529374782244 22.427136079887045 46-47 22.36302159328756 26.381866930022866 28.747291997935765 22.507819478753806 48-49 22.505494591854642 26.517037819033423 28.257526739473832 22.719940849638103 50-51 22.555208714333848 26.801239459827087 28.04076477670764 22.602787049131425 52-53 22.788803344693626 26.680893228095638 27.98928063415056 22.54102279306018 54-55 22.534834009624376 26.641402895695748 28.075759877822282 22.74800321685759 56-57 22.436471357995742 26.917779374403583 27.921950759448823 22.72379850815185 58-59 22.482312745629024 26.437034160321215 28.073764184349553 23.006888909700205 60-61 22.499502277734262 26.553613856587877 28.20169670900799 22.74518715666987 62-63 22.487484904607598 26.698182527852797 28.306775047873025 22.50755751966658 64-65 22.32172327617371 26.473229020951543 28.283600270342486 22.92144743253226 66-67 22.362137481368162 26.77487105063931 28.095897982653067 22.76709348533946 68-69 22.382341075970754 26.674573465116218 28.224290680281555 22.718794778631473 70-71 22.273890013857635 26.62303301470376 28.14914116713252 22.953935804306084 72-73 22.392361011057293 26.785906077188866 27.827717367101606 22.994015544652235 74-75 22.352191887356227 27.054709180958618 27.719896852359703 22.87320207932545 76-77 22.573014546588112 26.72185708036119 27.90188453367353 22.803243839377167 78-79 22.40159506888214 26.856766010284517 27.879716245916715 22.86192267491663 80-81 22.3756757730254 27.03465393544669 27.796791653978016 22.792878637549897 82-83 22.54518139356995 26.86819397546491 27.727812720209354 22.858811910755776 84-85 22.33237238008168 26.878541359410484 27.64765323951705 23.14143302099078 86-87 22.464039566300535 26.6894068984306 27.80967493496866 23.036878600300206 88-89 22.502492734640416 26.61998280872662 27.732307314477506 23.14521714215546 90-91 22.313314856485714 26.91881756907206 27.801652437922247 22.96621513651998 92-93 22.34775427444606 26.86651520738112 27.744457317101123 23.041273201071697 94-95 22.183317659447948 26.7545364764931 27.740026562651444 23.322119301407508 96-97 22.399954939156398 26.64749615951277 27.6592692873346 23.293279613996237 98-99 22.241666120898554 26.684360468923963 27.496037723492044 23.577935686685443 100-101 22.119469952410718 26.728619288705136 27.757881145666158 23.394029613217985 102-103 22.269338474717017 26.40636010467885 27.88891755885566 23.43538386174847 104-105 22.151424140577724 26.65256890178892 27.83446281359777 23.361544144035584 106-107 22.13185237092607 26.7202271216522 27.76201999430231 23.38590051311942 108-109 22.222807991847834 26.65368222790965 27.387036692609346 23.736473087633172 110-111 22.112257474684895 26.648183245860714 27.72336298277486 23.516196296679535 112-113 22.13508444251177 26.6612135516675 27.69853879221143 23.505163213609297 114-115 22.003777450037852 26.79297897254407 27.571586869562715 23.631656707855367 116-117 21.85999465603462 26.772906357485088 27.641955629369807 23.725143357110486 118-119 21.838153817136842 26.801165194019998 27.511074320412636 23.849606668430525 120-121 21.804511031991648 26.79604478352371 27.56902587551422 23.830418308970426 122-123 21.90586249704054 26.74912657056023 27.486412683089444 23.858598249309786 124-125 21.791155421652395 26.747611586301286 27.386422739474707 24.074810252571616 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 685.0 1 550.0 2 934.0 3 1308.0 4 806.5 5 517.0 6 971.5 7 2426.5 8 4082.0 9 4493.0 10 3224.5 11 1743.0 12 1357.0 13 1378.0 14 1439.0 15 1560.0 16 1693.0 17 1854.0 18 2070.0 19 2318.0 20 2625.5 21 3011.0 22 3563.0 23 4238.5 24 4942.0 25 6203.5 26 8041.0 27 10074.0 28 12407.5 29 14992.0 30 17905.5 31 20907.0 32 24074.0 33 26758.0 34 28855.5 35 31235.0 36 34739.5 37 38201.5 38 40549.0 39 42477.0 40 43119.0 41 44440.0 42 45952.5 43 47873.0 44 51194.5 45 53305.5 46 53300.0 47 53407.0 48 57104.5 49 58556.5 50 56680.0 51 54326.0 52 52615.5 53 52684.0 54 51401.0 55 47967.5 56 43679.5 57 39890.0 58 37584.5 59 35018.5 60 30378.5 61 24564.5 62 19363.0 63 14781.0 64 10881.5 65 8523.5 66 6542.0 67 5101.5 68 3974.0 69 2882.0 70 2073.5 71 1581.5 72 1313.5 73 1167.0 74 905.0 75 654.0 76 468.0 77 298.5 78 185.5 79 120.0 80 75.5 81 52.0 82 39.5 83 22.0 84 14.0 85 11.0 86 5.5 87 3.0 88 2.5 89 2.0 90 2.5 91 1.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09548408729524623 2 6.548977180743911E-5 3 0.0 4 0.10353932922756125 5 0.0 6 0.0 7 0.0020301829260306126 8 0.005370161288210007 9 0.0036019374494091513 10-11 5.566630603632325E-4 12-13 0.0 14-15 6.548977180743911E-5 16-17 2.9470397313347604E-4 18-19 0.0018009687247045757 20-21 0.0012115607784376236 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 3.2744885903719554E-5 52-53 0.0 54-55 0.0 56-57 0.00818622147592989 58-59 5.894079462669521E-4 60-61 0.0 62-63 0.0 64-65 2.6195908722975643E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0012115607784376236 76-77 0.0 78-79 0.0 80-81 6.548977180743911E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0012115607784376236 90-91 0.0 92-93 0.002161162469645491 94-95 0.0 96-97 0.008611904992678244 98-99 0.00366742722121659 100-101 0.0023903766709715275 102-103 0.0 104-105 0.0 106-107 0.0013752852079562214 108-109 0.0 110-111 1.3097954361487822E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 6.876426039781107E-4 122-123 0.004748008456039335 124-125 0.08575885618184152 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1526956.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.47201022374356 #Duplication Level Percentage of deduplicated Percentage of total 1 74.95818138296133 38.582482784970104 2 12.536633801122157 12.905714863653776 3 4.818274083058426 7.440187585919459 4 2.3897099788488765 4.920127058523655 5 1.3517178405991659 3.4787817255468414 6 0.8574832111940753 2.648183076796194 7 0.5820610811773316 2.097189774484199 8 0.42360317350750637 1.7442965502070868 9 0.31623673608149055 1.46496064614388 >10 1.6142599255079886 15.02417843949562 >50 0.10283285539083771 3.61531609848534 >100 0.047086689728511384 4.145184503755925 >500 0.001023595105235284 0.31012589721402933 >1k 6.397469407720526E-4 0.6885466670997403 >5k 2.55898776308821E-4 0.9347243277041237 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7694 0.5038783042864365 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6499 0.42561802697654677 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4613 0.30210431734771664 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1659 0.10864753142854149 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1604 0.10504559397913234 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 3.2744885903719554E-5 6.548977180743911E-5 12-13 0.0 0.0 0.0 6.548977180743911E-5 6.548977180743911E-5 14-15 0.0 0.0 0.0 1.3097954361487822E-4 6.548977180743911E-5 16-17 0.0 0.0 0.0 3.274488590371956E-4 6.548977180743911E-5 18-19 0.0 0.0 0.0 4.2568351674835423E-4 6.548977180743911E-5 20-21 0.0 0.0 0.0 7.531323757855498E-4 6.548977180743911E-5 22-23 0.0 0.0 0.0 0.0013097954361487823 6.548977180743911E-5 24-25 1.9646931542231734E-4 0.0 0.0 0.0020301829260306126 6.548977180743911E-5 26-27 1.9646931542231734E-4 0.0 0.0 0.0028160601877198817 6.548977180743911E-5 28-29 1.9646931542231734E-4 0.0 0.0 0.00366742722121659 6.548977180743911E-5 30-31 1.9646931542231734E-4 0.0 0.0 0.005959569234476959 6.548977180743911E-5 32-33 1.9646931542231734E-4 0.0 0.0 0.009987190200634464 6.548977180743911E-5 34-35 2.292142013260369E-4 0.0 0.0 0.015815779891496545 6.548977180743911E-5 36-37 2.6195908722975643E-4 0.0 0.0 0.0249516030586343 6.548977180743911E-5 38-39 3.274488590371956E-4 0.0 0.0 0.040996597151456884 6.548977180743911E-5 40-41 3.274488590371956E-4 0.0 0.0 0.060545294035977464 6.548977180743911E-5 42-43 3.274488590371956E-4 0.0 0.0 0.08078163352447615 6.548977180743911E-5 44-45 3.274488590371956E-4 0.0 0.0 0.10281894173767941 6.548977180743911E-5 46-47 3.6019374494091513E-4 0.0 0.0 0.1316016964470489 6.548977180743911E-5 48-49 3.929386308446347E-4 0.0 0.0 0.16434658235076846 6.548977180743911E-5 50-51 3.929386308446347E-4 0.0 0.0 0.2021014357977571 6.548977180743911E-5 52-53 3.929386308446347E-4 0.0 0.0 0.24018373810378296 6.548977180743911E-5 54-55 3.929386308446347E-4 0.0 0.0 0.27728369383269724 6.548977180743911E-5 56-57 3.929386308446347E-4 0.0 0.0 0.3237486869300753 6.548977180743911E-5 58-59 3.929386308446347E-4 0.0 0.0 0.37433953565132194 6.548977180743911E-5 60-61 3.929386308446347E-4 0.0 0.0 0.43462287060006966 6.548977180743911E-5 62-63 3.929386308446347E-4 0.0 0.0 0.499621469118953 6.548977180743911E-5 64-65 3.929386308446347E-4 0.0 0.0 0.5678618113423046 6.548977180743911E-5 66-67 3.929386308446347E-4 0.0 0.0 0.6405227131626583 6.548977180743911E-5 68-69 3.929386308446347E-4 0.0 0.0 0.7343695561627186 6.548977180743911E-5 70-71 3.929386308446347E-4 0.0 0.0 0.8388584870814876 6.548977180743911E-5 72-73 3.929386308446347E-4 0.0 0.0 0.9522540269660684 6.548977180743911E-5 74-75 3.929386308446347E-4 0.0 0.0 1.0618839049717215 6.548977180743911E-5 76-77 3.929386308446347E-4 0.0 0.0 1.1803549021713788 6.548977180743911E-5 78-79 3.929386308446347E-4 0.0 0.0 1.3232863291411148 6.548977180743911E-5 80-81 5.894079462669521E-4 0.0 0.0 1.482622943948614 6.548977180743911E-5 82-83 6.548977180743912E-4 0.0 0.0 1.658462981251588 6.548977180743911E-5 84-85 7.203874898818303E-4 0.0 0.0 1.833386161749258 6.548977180743911E-5 86-87 8.186221475929889E-4 0.0 0.0 2.011452851293685 6.548977180743911E-5 88-89 8.513670334967085E-4 0.0 0.0 2.212539195628427 6.548977180743911E-5 90-91 9.823465771115866E-4 0.0 0.0 2.4468615991554437 6.548977180743911E-5 92-93 9.823465771115866E-4 0.0 0.0 2.711112828398461 6.548977180743911E-5 94-95 9.823465771115866E-4 0.0 0.0 2.9682256725144667 6.548977180743911E-5 96-97 9.823465771115866E-4 0.0 0.0 3.2316582796098903 6.548977180743911E-5 98-99 0.0011788158925339041 0.0 0.0 3.5164405523145392 6.548977180743911E-5 100-101 0.0014080300938599409 0.0 0.0 3.8620300781423955 6.548977180743911E-5 102-103 0.0015717545233785387 0.0 0.0 4.234470410411301 6.548977180743911E-5 104-105 0.0016699891810896973 0.0 0.0 4.6033088052307995 6.548977180743911E-5 106-107 0.001768223838800856 0.0 0.0 4.9678903648828125 6.548977180743911E-5 108-109 0.0018009687247045754 0.0 0.0 5.358373129284669 6.548977180743911E-5 110-111 0.0018992033824157342 0.0 0.0 5.793618152716908 6.548977180743911E-5 112-113 0.0019646931542231733 0.0 0.0 6.27041643636097 6.548977180743911E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5125 0.0 47.584194 1 GTATCAA 8265 0.0 45.738266 1 ATCAACG 11075 0.0 34.25551 3 CAACGCA 11230 0.0 33.907352 5 TCAACGC 11195 0.0 33.835125 4 TATCAAC 11175 0.0 33.6825 2 AACGCAG 11355 0.0 33.481693 6 GTACATG 9020 0.0 30.804394 1 ACGCAGA 13230 0.0 28.60164 7 TACATGG 9540 0.0 28.467625 2 CGCAGAG 13245 0.0 28.254807 8 ACATGGG 9240 0.0 28.167236 3 AGTACTT 8230 0.0 27.218163 12-13 GAGTACT 7930 0.0 26.334652 12-13 CATGGGG 3555 0.0 25.967302 4 GCAGAGT 14120 0.0 25.787567 9 GTACTTT 8440 0.0 24.884329 14-15 AGAGTAC 13300 0.0 24.13344 10-11 TACTTTT 9410 0.0 23.868273 14-15 CAGAGTA 13530 0.0 22.18415 10-11 >>END_MODULE