FastQCFastQC Report
Thu 26 May 2016
SRR1364531_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364531_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500112
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27090.1805865162067899No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20070.13379001034589416No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23700.057.4961361
GTATCAA46850.055.3768731
ATCAACG55100.046.2168433
TCAACGC55800.045.3171774
CAACGCA56300.044.914715
AACGCAG58400.043.4990436
TATCAAC58900.043.3361242
GTACATG77550.037.7515531
ACGCAGA67650.037.111577
CGCAGAG68050.035.9341438
TACATGG80350.035.3216172
ACATGGG81100.033.601043
GCAGAGT76650.030.8933149
CATGGGG36250.027.0822074
CATGGGA44600.026.4142574
GAGTACT36300.025.7400712-13
AGAGTAC71700.024.64963510-11
CAGAGTA75650.024.2671910-11
GTATAGG5950.023.0018311
GTACTTT39350.022.9135214-15
Produced by FastQC (version 0.11.5)