##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364531_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1500112 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.354737512932367 31.0 28.0 33.0 16.0 34.0 2 28.368829794042046 31.0 28.0 34.0 16.0 34.0 3 28.5684315571104 31.0 28.0 34.0 16.0 34.0 4 31.89538781104344 35.0 32.0 37.0 19.0 37.0 5 32.03351616412641 35.0 32.0 37.0 20.0 37.0 6 32.01394362554262 35.0 32.0 37.0 19.0 37.0 7 31.81833889736233 35.0 32.0 37.0 17.0 37.0 8 31.686503407745555 35.0 32.0 37.0 17.0 37.0 9 32.80809632880745 37.0 32.0 39.0 16.0 39.0 10-11 32.73744860383758 37.0 32.0 39.0 15.0 39.0 12-13 32.64380859562486 37.0 32.0 39.0 12.5 39.0 14-15 33.36719158302847 38.0 32.0 41.0 10.5 41.0 16-17 33.47473755292938 38.0 32.0 41.0 11.0 41.0 18-19 33.30154881768828 38.0 32.0 41.0 10.0 41.0 20-21 33.03164363727508 38.0 31.5 41.0 10.0 41.0 22-23 32.93275302110776 38.0 31.5 40.0 10.0 41.0 24-25 32.76812664654372 38.0 31.0 40.0 10.0 41.0 26-27 32.66937402007317 38.0 31.0 40.0 9.0 41.0 28-29 32.72849293919387 38.0 31.0 40.0 8.5 41.0 30-31 32.66527365956675 38.0 31.0 40.0 8.0 41.0 32-33 32.627250831937886 38.0 31.0 40.0 8.0 41.0 34-35 32.159478758919334 38.0 30.0 40.0 4.5 41.0 36-37 32.01059087588126 38.0 30.0 40.0 2.0 41.0 38-39 31.807037407873544 37.5 30.0 40.0 2.0 41.0 40-41 31.99083001802532 38.0 30.0 40.0 2.0 41.0 42-43 31.85701200976994 38.0 30.0 40.0 2.0 41.0 44-45 31.68538049158996 37.0 30.0 40.0 2.0 41.0 46-47 31.617992523224935 37.0 30.0 40.0 2.0 41.0 48-49 31.43316099064603 37.0 30.0 40.0 2.0 41.0 50-51 31.272168011455143 37.0 30.0 40.0 2.0 41.0 52-53 31.20360679735913 37.0 29.5 40.0 2.0 41.0 54-55 31.224203259489958 37.0 30.0 40.0 2.0 41.0 56-57 31.093854992160587 37.0 30.0 40.0 2.0 41.0 58-59 30.88895229156223 36.0 29.0 40.0 2.0 41.0 60-61 30.680399863476858 36.0 29.0 40.0 2.0 41.0 62-63 30.39216238520857 35.5 29.0 39.5 2.0 41.0 64-65 30.112245618993782 35.0 28.0 39.0 2.0 41.0 66-67 29.856985345094234 35.0 28.0 39.0 2.0 41.0 68-69 29.802439084548354 35.0 29.0 39.0 2.0 40.0 70-71 29.559033258850008 35.0 29.0 38.0 2.0 40.0 72-73 29.277760927184104 35.0 28.5 37.0 2.0 40.0 74-75 28.97656241667289 35.0 28.0 37.0 2.0 39.0 76-77 28.66928735987713 34.0 28.0 36.0 2.0 39.0 78-79 28.363435530147083 34.0 28.0 36.0 2.0 39.0 80-81 28.072615244728393 34.0 27.0 35.5 2.0 37.0 82-83 27.83266016137462 34.0 27.0 35.0 2.0 37.0 84-85 27.589996946894633 34.0 26.5 35.0 2.0 36.5 86-87 27.40017778672526 34.0 26.0 35.0 2.0 36.0 88-89 27.20698721162153 34.0 26.0 35.0 2.0 36.0 90-91 27.071379003701054 34.0 26.0 35.0 2.0 36.0 92-93 26.947514918886057 34.0 26.0 35.0 2.0 35.0 94-95 26.823408518830597 34.0 25.5 35.0 2.0 35.0 96-97 26.710789927685397 34.0 25.0 35.0 2.0 35.0 98-99 26.622565848416652 34.0 25.0 35.0 2.0 35.0 100-101 26.52787558528963 34.0 25.0 35.0 2.0 35.0 102-103 25.386015844150304 31.5 23.5 34.0 2.0 35.0 104-105 26.238733507898075 33.0 24.5 35.0 2.0 35.0 106-107 26.37891970732852 34.0 25.0 35.0 2.0 35.0 108-109 26.332021209083056 34.0 25.0 35.0 2.0 35.0 110-111 26.245246354938832 34.0 24.5 35.0 2.0 35.0 112-113 26.155017092057125 34.0 24.0 35.0 2.0 35.0 114-115 26.029839105346802 33.0 24.0 35.0 2.0 35.0 116-117 25.923429717247778 33.0 23.5 35.0 2.0 35.0 118-119 25.776592347771366 33.0 23.0 35.0 2.0 35.0 120-121 25.598391320114764 33.0 19.5 35.0 2.0 35.0 122-123 25.36202596872767 33.0 18.0 35.0 2.0 35.0 124-125 23.956943214906623 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7826242949741342 1101 2 0.8135877816958015 1101 3 0.8166268822372231 1101 4 0.926407163269193 1101 5 0.8536645667591998 1101 6 0.9168437908690379 1101 7 1.035273316041497 1101 8 0.932041685280435 1101 9 1.2572103845780447 1101 10-11 1.3261134468464775 1101 12-13 1.1799826944486682 1101 14-15 1.4438521190164053 1101 16-17 1.3002468513175671 1101 18-19 1.6252907112069437 1101 20-21 1.8521188174995586 1101 22-23 1.7341364705518387 1101 24-25 1.86251902635982 1101 26-27 1.9533565788260319 1101 28-29 1.6352196773351295 1101 30-31 1.7972928740902603 1101 32-33 1.8282559436869548 1101 34-35 2.129486791007807 1101 36-37 2.183805908344109 1101 38-39 1.8300230246581002 1101 40-41 1.6792972415204446 1101 42-43 1.7932365649242783 1101 44-45 1.7493261987448285 1101 46-47 1.5299802142558931 1101 48-49 1.5019477065215021 1101 50-51 1.439343683732755 1101 52-53 1.277737087873632 1101 54-55 1.2785468043912367 1101 56-57 1.1755742162181306 1101 58-59 1.178522817753766 1101 60-61 1.0943115923469442 1101 62-63 0.9333073849130464 1101 64-65 1.1337176114960847 1101 66-67 1.0672286652452136 1101 68-69 0.9670376412265611 1101 70-71 0.9274293398592519 1101 72-73 0.960522931096726 1101 74-75 0.9707164239097423 1101 76-77 1.0855270892357467 1101 78-79 1.1214906652113967 1101 80-81 1.0953848599845308 1101 82-83 1.035080273249477 1101 84-85 1.0027011687615257 1101 86-87 0.9080226368438069 1101 88-89 0.834876407537358 1101 90-91 0.8783603612772239 1101 92-93 0.7379150020418166 1101 94-95 0.8796062722638638 1101 96-97 0.8361646441790782 1101 98-99 0.8724938902970756 1101 100-101 0.9636818647897414 1101 102-103 0.9712426753317409 1101 104-105 0.9268320681686184 1101 106-107 0.9726039406353486 1101 108-109 1.0273893115997232 1101 110-111 0.7942664357768798 1101 112-113 0.8107252567199268 1101 114-115 0.9521628236966748 1101 116-117 1.0455434295001957 1101 118-119 1.0288750191566187 1101 120-121 1.0838533016093983 1101 122-123 1.1441662764291394 1101 124-125 1.2035541599549298 1102 1 0.1137608925606699 1102 2 0.26713542466190887 1102 3 0.5186568086923096 1102 4 0.556047128047009 1102 5 0.7651688269072778 1102 6 0.08158036861208728 1102 7 -0.5105248261122632 1102 8 0.22866457225938674 1102 9 0.5976393138980285 1102 10-11 0.7377246757782814 1102 12-13 0.6336094012983935 1102 14-15 1.0999397280005425 1102 16-17 1.002701552363071 1102 18-19 1.1486068055131042 1102 20-21 1.5495335835324724 1102 22-23 1.331661770994593 1102 24-25 1.385156101430212 1102 26-27 1.108440386542668 1102 28-29 1.4338803971575302 1102 30-31 1.3537840189352934 1102 32-33 1.1446393756176008 1102 34-35 1.5124048064113111 1102 36-37 1.7078048975666142 1102 38-39 1.6536532041176137 1102 40-41 1.436940858739625 1102 42-43 1.3178502370851106 1102 44-45 1.2601626016171714 1102 46-47 0.8475953374715246 1102 48-49 0.583130037138762 1102 50-51 0.915346244782473 1102 52-53 0.7928971999893157 1102 54-55 0.7504667235290405 1102 56-57 0.5792100696739979 1102 58-59 0.6930744480200417 1102 60-61 0.7387558817641633 1102 62-63 0.6152690373792211 1102 64-65 0.5685100610502225 1102 66-67 0.4877291551312375 1102 68-69 0.38390429301061246 1102 70-71 0.3643309347793213 1102 72-73 0.6362602405682694 1102 74-75 0.4328618766512413 1102 76-77 0.5780609781866453 1102 78-79 0.4097382064796413 1102 80-81 0.0796216919722248 1102 82-83 0.3164694101819201 1102 84-85 -0.11747128352109826 1102 86-87 0.2353362631165794 1102 88-89 0.3893909552919439 1102 90-91 0.07525113529714389 1102 92-93 0.4319680027487429 1102 94-95 0.2624669275949394 1102 96-97 0.41531999385848195 1102 98-99 0.4661357588091839 1102 100-101 0.45299428805126496 1102 102-103 0.21002025467467078 1102 104-105 0.38401509950892176 1102 106-107 0.2555580773817603 1102 108-109 -0.22362766698171654 1102 110-111 0.025112047266258486 1102 112-113 -0.23063213063729293 1102 114-115 -0.21569290176871192 1102 116-117 -0.34489883216095407 1102 118-119 0.086294777066108 1102 120-121 0.4193376376588063 1102 122-123 0.30112746381364275 1102 124-125 0.6009703212328503 1103 1 0.771179050583843 1103 2 0.8369719427680167 1103 3 0.659317606299922 1103 4 0.8040098215969529 1103 5 0.9222403747161749 1103 6 0.8961594470330354 1103 7 1.0365453849935662 1103 8 1.223283953832162 1103 9 1.3357935601063033 1103 10-11 1.3522208708374421 1103 12-13 1.2579367754229267 1103 14-15 1.5983096496946132 1103 16-17 1.4299046643943285 1103 18-19 1.5380847763356797 1103 20-21 1.84022472896266 1103 22-23 1.7691920799595167 1103 24-25 1.937135004836314 1103 26-27 1.8328333912335637 1103 28-29 1.989093616840016 1103 30-31 1.9484937056804839 1103 32-33 1.8436614452069904 1103 34-35 2.1823978819785452 1103 36-37 2.1426328514472495 1103 38-39 2.1742123967489597 1103 40-41 1.8856901476446808 1103 42-43 1.710363767317915 1103 44-45 1.5151822325006101 1103 46-47 1.558088961708755 1103 48-49 1.7215714802185254 1103 50-51 1.663658657158475 1103 52-53 1.5883779390306856 1103 54-55 1.487761632866377 1103 56-57 1.4706874607404714 1103 58-59 1.4260503298554923 1103 60-61 1.229640071986335 1103 62-63 1.1849922854433323 1103 64-65 1.4313365430031801 1103 66-67 1.4325221526723233 1103 68-69 1.5240447867203386 1103 70-71 1.3995996898113852 1103 72-73 1.4441389005700742 1103 74-75 1.4552949793255792 1103 76-77 1.4039573463954973 1103 78-79 1.4130193462210698 1103 80-81 1.4572175771028064 1103 82-83 1.4681102131985178 1103 84-85 1.4078868285567907 1103 86-87 1.2624317006070598 1103 88-89 1.349875642270277 1103 90-91 1.2894659621319242 1103 92-93 1.3555958226482119 1103 94-95 1.3405682730021233 1103 96-97 1.4350590958427105 1103 98-99 1.3090997657476073 1103 100-101 1.2855751430438502 1103 102-103 1.111722452774064 1103 104-105 1.2660698996718125 1103 106-107 1.3823682608831156 1103 108-109 1.364555295027941 1103 110-111 1.389076086845101 1103 112-113 1.4339426345848025 1103 114-115 1.5740843117960246 1103 116-117 1.5323560771142084 1103 118-119 1.5380357162399 1103 120-121 1.415779831468317 1103 122-123 1.495787179624351 1103 124-125 1.5902475534492595 1104 1 0.5458836143031043 1104 2 0.6804592518655816 1104 3 0.4295644946073942 1104 4 0.560640636376835 1104 5 0.5172953750163671 1104 6 0.4673050316377285 1104 7 0.5233000309221225 1104 8 0.6027393687594618 1104 9 0.937339586825594 1104 10-11 0.6956213773306388 1104 12-13 0.6917604590795108 1104 14-15 0.6819394380337016 1104 16-17 0.843045095817736 1104 18-19 1.1055056250192052 1104 20-21 1.3657960528542787 1104 22-23 1.1336299209762117 1104 24-25 1.6719058495775556 1104 26-27 1.6154831058338317 1104 28-29 1.473358113055788 1104 30-31 1.4560679562665868 1104 32-33 1.546065407167191 1104 34-35 1.7678579393984677 1104 36-37 1.7919707645824339 1104 38-39 1.738887389714332 1104 40-41 1.4574446163512533 1104 42-43 1.4213295403276547 1104 44-45 1.2940506086481243 1104 46-47 1.1620754074820852 1104 48-49 1.116771983384293 1104 50-51 1.0180726057583165 1104 52-53 1.0676973993788934 1104 54-55 0.9904839795458535 1104 56-57 0.939983571188101 1104 58-59 0.8614762449176965 1104 60-61 0.955668086080184 1104 62-63 1.0098195663445182 1104 64-65 0.8786025141398675 1104 66-67 0.9849001100189732 1104 68-69 0.9920981732154424 1104 70-71 0.8771836405885232 1104 72-73 0.6940658417113994 1104 74-75 0.7628570413793128 1104 76-77 0.8967715374639411 1104 78-79 0.9346125790900288 1104 80-81 0.8609382984035143 1104 82-83 0.8163031276465915 1104 84-85 0.8546055816084461 1104 86-87 0.8947485272195799 1104 88-89 0.9431897078366234 1104 90-91 1.005562311576064 1104 92-93 1.0010516701418197 1104 94-95 0.9406891094609513 1104 96-97 0.8085477359135389 1104 98-99 0.9334522725706513 1104 100-101 0.9167964484537521 1104 102-103 0.9097756511968633 1104 104-105 0.980745760663229 1104 106-107 0.9227831917990947 1104 108-109 1.0685757746557307 1104 110-111 0.9475698301857136 1104 112-113 0.9098371896744375 1104 114-115 0.9525811004613409 1104 116-117 1.086985115334123 1104 118-119 0.9732930895786254 1104 120-121 0.7889310264146694 1104 122-123 0.9061866379761021 1104 124-125 1.1855191985828526 1105 1 0.23451432681275008 1105 2 0.46079800768234236 1105 3 0.531701789705334 1105 4 0.5894224093496341 1105 5 0.647653227744108 1105 6 0.5620802726875098 1105 7 0.6667373190048735 1105 8 0.3656726542855573 1105 9 0.3398401129649713 1105 10-11 0.6691780016203523 1105 12-13 0.6116586608520223 1105 14-15 0.7587401987922746 1105 16-17 0.6409705477130103 1105 18-19 0.7232848491389916 1105 20-21 1.0719550015108723 1105 22-23 1.312994734333138 1105 24-25 1.3056154050482434 1105 26-27 1.1761625661317439 1105 28-29 0.9254774804413799 1105 30-31 0.9505707740352847 1105 32-33 1.0586401384463713 1105 34-35 1.6627956990580017 1105 36-37 1.655817583972425 1105 38-39 1.7007091570749786 1105 40-41 1.2410668716574662 1105 42-43 0.9566744149505908 1105 44-45 1.084522419269124 1105 46-47 0.9861266027928153 1105 48-49 0.7363712600400696 1105 50-51 0.76543021685168 1105 52-53 0.7939373345273424 1105 54-55 0.6930983076853465 1105 56-57 0.6313014918852531 1105 58-59 0.5722827581335359 1105 60-61 0.5711712701977909 1105 62-63 0.7276159387108976 1105 64-65 0.7413366461657738 1105 66-67 0.5472339863801139 1105 68-69 0.4127855840640464 1105 70-71 0.5439230573922451 1105 72-73 0.5529065422952186 1105 74-75 0.6841543841805588 1105 76-77 0.7530711796891616 1105 78-79 0.6657319698595998 1105 80-81 0.5972099578081576 1105 82-83 0.4773193171503074 1105 84-85 0.458518109961517 1105 86-87 0.4981368648529667 1105 88-89 0.6670269397978394 1105 90-91 0.5850015639125132 1105 92-93 0.5345998762013906 1105 94-95 0.6602568177536874 1105 96-97 0.7705935996785165 1105 98-99 0.6466264130147898 1105 100-101 0.5798665302197108 1105 102-103 0.5502042014685067 1105 104-105 0.7239955872320998 1105 106-107 0.5395471617740739 1105 108-109 0.5486156053557707 1105 110-111 0.6592123204229061 1105 112-113 0.5677452594833916 1105 114-115 0.5768201470188288 1105 116-117 0.6323617142132498 1105 118-119 0.662531785376526 1105 120-121 0.5422398585411301 1105 122-123 0.7892960048160695 1105 124-125 0.8346056647189641 1106 1 0.37501012819718227 1106 2 0.5109192434141896 1106 3 0.28907551815408894 1106 4 0.5689171223325751 1106 5 0.48286975246097086 1106 6 0.34561269598916056 1106 7 0.13225076446924078 1106 8 0.3437255365048877 1106 9 0.5861609617501884 1106 10-11 0.6284300350252607 1106 12-13 0.6376942213860914 1106 14-15 0.9938316358364077 1106 16-17 0.7939179762047388 1106 18-19 0.7516302963091661 1106 20-21 1.0944133686019342 1106 22-23 1.108826806505725 1106 24-25 1.2355696856133918 1106 26-27 1.474012434636343 1106 28-29 1.179017158535963 1106 30-31 0.896488063554095 1106 32-33 1.0447094390616982 1106 34-35 1.4061165978636794 1106 36-37 1.2926854412235151 1106 38-39 1.3847064238399618 1106 40-41 1.2036396979853237 1106 42-43 1.0092922925567152 1106 44-45 0.8905337472613333 1106 46-47 0.6642531282800661 1106 48-49 0.9191668490383442 1106 50-51 0.8999471122676503 1106 52-53 0.890072925037181 1106 54-55 0.5420304803004932 1106 56-57 0.5501647384908921 1106 58-59 0.5292980187565881 1106 60-61 0.6035859004169417 1106 62-63 0.5578396360702911 1106 64-65 0.595353563305963 1106 66-67 0.6242046192558277 1106 68-69 0.631134678711895 1106 70-71 0.706732276054332 1106 72-73 0.6564635997628905 1106 74-75 0.6969324222026856 1106 76-77 0.7454968217127771 1106 78-79 0.8353227038788802 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78-79 -2.9032558364779675 2216 80-81 -2.758602735027825 2216 82-83 -2.4971719589843318 2216 84-85 -2.4307335965917325 2216 86-87 -2.4922841868425465 2216 88-89 -2.697709980703067 2216 90-91 -2.6609127310437692 2216 92-93 -2.542389731636881 2216 94-95 -2.691872932537269 2216 96-97 -2.714572272146672 2216 98-99 -2.767973486720688 2216 100-101 -2.7740756513153926 2216 102-103 -2.574695617381 2216 104-105 -2.804970057904871 2216 106-107 -2.838748441445208 2216 108-109 -2.846422986850353 2216 110-111 -2.903982579203266 2216 112-113 -2.8116656290358755 2216 114-115 -2.891537708387581 2216 116-117 -2.90377992109142 2216 118-119 -2.9843633307483906 2216 120-121 -2.9983876506192146 2216 122-123 -3.193824837643028 2216 124-125 -3.1888295918577967 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 95902.0 3 16381.0 4 10340.0 5 8089.0 6 7549.0 7 8085.0 8 8481.0 9 9168.0 10 9812.0 11 10572.0 12 11576.0 13 12817.0 14 14611.0 15 12908.0 16 9369.0 17 8318.0 18 7649.0 19 7090.0 20 6869.0 21 7524.0 22 8885.0 23 11850.0 24 15682.0 25 20037.0 26 24769.0 27 27677.0 28 32061.0 29 37401.0 30 42496.0 31 48639.0 32 59626.0 33 75036.0 34 97589.0 35 132929.0 36 204713.0 37 277770.0 38 99442.0 39 400.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.34599283552714 18.436816829910775 12.967255838885366 23.249934495676712 2 15.31953446140267 19.780390473231797 41.19021936953093 23.709855695834605 3 19.747596634621793 25.372938305844077 28.10110801477353 26.778357044760597 4 12.017525613743153 16.31046096737548 37.640868920569396 34.031144498311974 5 13.30209077144326 38.70904080801292 33.82330684449458 14.165561576049235 6 31.721485234317715 36.61415905606293 17.743483767748817 13.92087194187054 7 28.822380785079478 32.59377827081449 20.445786369475755 18.13805457463028 8 26.170645925104257 35.81819224164596 19.45721386136502 18.553947971884767 9 26.657031605710905 16.67487735629571 19.477066843902012 37.19102419409137 10-11 25.004066363044892 27.159272107682625 27.029781776294037 20.806879752978443 12-13 25.81401581133592 24.52237006045746 27.35675101645731 22.306863111749305 14-15 22.654876933211366 25.816289247624407 25.549593422214162 25.979240396950065 16-17 22.344791439877866 29.130201250090437 25.859708072175437 22.665299237856264 18-19 22.19733011917432 27.66312262525504 28.204245316508775 21.935301939061866 20-21 23.08583851503109 26.859794492100285 28.348770092620555 21.705596900248068 22-23 22.68552513471723 26.768189701060574 28.13771072534817 22.408574438874023 24-25 22.324541198008717 26.892126946495825 28.408355273114438 22.374976582381016 26-27 22.08195845804166 27.243599787443003 28.158034927888636 22.516406826626696 28-29 22.183284259056187 27.367347795857228 28.167724035685453 22.28164390940113 30-31 22.420021465378746 27.071975681459115 28.073115613063216 22.434887240098927 32-33 22.136324923790777 27.049448910237285 28.085260994711604 22.72896517126033 34-35 22.500137502062532 27.04743904491901 28.218456610182486 22.233966842835976 36-37 22.639836421104985 26.791744252637923 28.07815465725835 22.490264668998748 38-39 22.358763878963703 27.016782746888236 28.109434495557668 22.515018878590396 40-41 22.6312711745177 26.99669742535201 27.90148819227879 22.4705432078515 42-43 22.403388868907 26.968115018338708 27.808932114953006 22.81956399780129 44-45 22.52588922106981 27.192616338145363 27.765939572704507 22.51555486808032 46-47 22.75220666982433 27.43613648704097 27.169646169522508 22.64201067361219 48-49 22.71325988382812 27.35823486483342 27.15923642474915 22.76926882658931 50-51 22.69918712194771 27.600521452587444 27.007637460455236 22.69265396500961 52-53 22.848060678136033 27.491980598781957 26.993217839734633 22.666740883347376 54-55 22.724460330445716 27.402818809576672 27.04845074817332 22.82427011180429 56-57 22.731821069606486 27.64726705229903 26.876591200142396 22.74432067795209 58-59 22.783751975273788 27.399351446912007 26.952083799271854 22.86481277854235 60-61 22.758592874508935 27.407601474883187 27.11557807034561 22.71822758026227 62-63 22.58741741350276 27.446072271707532 27.235532339300356 22.73097797548935 64-65 22.553701750432374 27.356089709825866 27.146303183368026 22.943905356373737 66-67 22.587774850542104 27.581448555018106 26.942586034952615 22.888190559487175 68-69 22.719497481841824 27.290653827677104 27.215010736384993 22.77483795409608 70-71 22.58129781856992 27.242710511702445 27.265342773775377 22.910648895952257 72-73 22.616558617323985 27.536381649301433 26.928220746007153 22.918838987367426 74-75 22.63757639429589 27.64926885459219 26.872326866260654 22.840827884851265 76-77 22.809869532652836 27.420042459136663 26.96638501683799 22.80370299137251 78-79 22.80738710998837 27.513208290516726 26.81152150240701 22.867883097087894 80-81 22.5824031507989 27.569323708855087 26.965982784734514 22.88229035561149 82-83 22.77166406237501 27.708874412701125 26.875823285033945 22.643638239889928 84-85 22.832028541868873 27.692698945145427 26.730304137291082 22.744968375694615 86-87 22.679082846232614 27.552745541428447 26.923025642119637 22.845145970219303 88-89 22.695785170858027 27.568437552296167 26.776802805041232 22.958974471804574 90-91 22.72842288867871 27.731024920497177 26.847434256607343 22.69311793421677 92-93 22.71986688390474 27.58465751671284 26.96090914666503 22.734566452717388 94-95 22.571994283064328 27.573012783092167 26.948477234107315 22.90651569973619 96-97 22.795923463089512 27.386544601248858 26.884861601298525 22.932670334363102 98-99 22.53744586190645 27.548281813400315 26.731735705443445 23.182536619249795 100-101 22.417546593012524 27.533669537031574 26.986496851465365 23.062287018490537 102-103 22.50045329948697 27.309694209499025 27.091510500549294 23.098341990464714 104-105 22.47901379414936 27.30934925679583 27.008767792652787 23.202869156402024 106-107 22.39486864809716 27.520187666050155 26.963662367763995 23.121281318088688 108-109 22.40042743475154 27.43105181479783 26.75896866367311 23.40955208677752 110-111 22.34182898351511 27.542512137516074 26.895522368160545 23.220136510808274 112-113 22.401294036711924 27.59737272950286 26.940021811704728 23.061311422080486 114-115 22.408526829996696 27.77259297972418 26.7427698731828 23.076110317096322 116-117 22.313867231246736 27.737028968503687 26.91162393207974 23.037479868169843 118-119 22.308834273707564 27.77409286773254 26.709305705174014 23.20776715338588 120-121 22.21280811032776 27.847620710986913 26.888792303508 23.05077887517732 122-123 22.275370105698773 27.808323645167825 26.798132406113677 23.118173843019722 124-125 22.306016663910686 27.828137418587406 26.631719039211397 23.23412687829051 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 946.0 1 1000.0 2 1178.5 3 1102.5 4 754.0 5 540.5 6 492.5 7 563.0 8 724.5 9 838.5 10 797.0 11 752.5 12 780.5 13 862.5 14 970.0 15 1097.5 16 1246.0 17 1376.0 18 1529.0 19 1789.5 20 2113.5 21 2540.5 22 3155.0 23 3884.0 24 4878.5 25 6105.5 26 7979.5 27 10439.0 28 12699.0 29 15279.5 30 18057.0 31 20914.5 32 24293.0 33 27298.5 34 30089.0 35 32923.5 36 36254.0 37 39575.0 38 41136.5 39 42109.0 40 43055.5 41 44087.0 42 45215.5 43 46891.0 44 49246.0 45 51296.5 46 52036.0 47 52094.0 48 53063.0 49 53815.5 50 53469.0 51 52383.5 52 51817.5 53 51885.5 54 51250.5 55 48811.5 56 45298.0 57 42031.5 58 39685.5 59 36033.5 60 30510.5 61 24750.5 62 19523.0 63 15083.5 64 11049.0 65 8155.0 66 6219.5 67 4802.5 68 3676.0 69 2838.5 70 2207.5 71 1724.0 72 1335.0 73 1126.0 74 879.0 75 614.5 76 462.5 77 297.5 78 195.5 79 147.5 80 109.0 81 74.5 82 57.5 83 43.0 84 33.5 85 31.0 86 23.0 87 17.0 88 12.5 89 7.5 90 8.0 91 5.5 92 5.5 93 8.0 94 6.0 95 3.0 96 3.0 97 3.0 98 1.5 99 2.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014065616433972928 2 0.005732905276406028 3 0.008799342982390648 4 0.00906598973943279 5 0.015665496976225775 6 7.999402711264225E-4 7 0.00739944750791941 8 0.0 9 0.0023331591241187322 10-11 0.0 12-13 0.0032664227737662257 14-15 0.018398626235907718 16-17 0.030697707904476467 18-19 0.005499589363994155 20-21 0.00806606440052476 22-23 0.001699873076143648 24-25 0.011532472242072593 26-27 0.018598611303689325 28-29 3.3330844630267606E-4 30-31 0.0014665571637317747 32-33 0.00863268875923931 34-35 0.008965997205541986 36-37 0.010865855349467239 38-39 0.0 40-41 0.024864810094179634 42-43 0.009499290719626269 44-45 0.017332039207739156 46-47 0.003833047132480775 48-49 0.02343158377507813 50-51 0.004766310782128268 52-53 0.0 54-55 0.01726537751847862 56-57 0.004333009801934789 58-59 2.3331591241187324E-4 60-61 0.0040663630448926485 62-63 0.026431359791802212 64-65 0.016932069072175947 66-67 0.001599880542252845 68-69 0.02036514606909351 70-71 0.002733129259681944 72-73 0.011999104066896337 74-75 0.0 76-77 0.005432927674733619 78-79 8.3327111575669E-4 80-81 0.00319976108450569 82-83 0.001599880542252845 84-85 0.0 86-87 0.005332935140842817 88-89 0.016065467111788988 90-91 0.021498394786522605 92-93 0.004532994869716395 94-95 0.02383155391064134 96-97 0.017365370052369423 98-99 8.666019603869578E-4 100-101 0.008566027069978775 102-103 0.0 104-105 0.0012999029405804368 106-107 3.333084463026761E-5 108-109 0.0 110-111 7.332785818658874E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.022931621105624113 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1500112.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.27481400580015 #Duplication Level Percentage of deduplicated Percentage of total 1 82.52833234286095 55.52078208574832 2 10.647770659375015 14.326535813717403 3 3.078207952197353 6.212576023657556 4 1.3179204010726557 3.5465139942644983 5 0.669832377291576 2.2531424298676863 6 0.38772903957184685 1.5650639413106113 7 0.2608651983143456 1.2284760388028644 8 0.18507804043563203 0.9960872597492084 9 0.13347855162646932 0.8081770260990886 >10 0.7294331680263656 8.897340099052913 >50 0.04243848264655346 1.9517141976778558 >100 0.01820959580493847 2.107706310974372 >500 3.0179604695134164E-4 0.11721756236138701 >1k 4.0239472926845556E-4 0.4686672167162847 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2709 0.1805865162067899 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2007 0.13379001034589416 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.666168926053521E-5 2 6.666168926053521E-5 0.0 0.0 0.0 6.666168926053521E-5 3 6.666168926053521E-5 0.0 0.0 0.0 6.666168926053521E-5 4 6.666168926053521E-5 6.666168926053521E-5 0.0 0.0 6.666168926053521E-5 5 6.666168926053521E-5 6.666168926053521E-5 0.0 0.0 6.666168926053521E-5 6 6.666168926053521E-5 6.666168926053521E-5 0.0 0.0 6.666168926053521E-5 7 6.666168926053521E-5 6.666168926053521E-5 0.0 0.0 6.666168926053521E-5 8 6.666168926053521E-5 6.666168926053521E-5 0.0 0.0 6.666168926053521E-5 9 6.666168926053521E-5 6.666168926053521E-5 0.0 0.0 6.666168926053521E-5 10-11 9.999253389080281E-5 6.666168926053521E-5 0.0 0.0 6.666168926053521E-5 12-13 1.3332337852107041E-4 6.666168926053521E-5 0.0 9.999253389080282E-5 9.999253389080281E-5 14-15 1.9998506778160565E-4 6.666168926053521E-5 0.0 2.3331591241187324E-4 1.3332337852107041E-4 16-17 2.3331591241187324E-4 6.666168926053521E-5 0.0 3.3330844630267606E-4 1.3332337852107041E-4 18-19 3.3330844630267606E-4 6.666168926053521E-5 0.0 3.3330844630267606E-4 1.3332337852107041E-4 20-21 3.3330844630267606E-4 6.666168926053521E-5 0.0 4.3330098019347886E-4 1.9998506778160565E-4 22-23 3.3330844630267606E-4 6.666168926053521E-5 0.0 8.332711157566902E-4 1.9998506778160565E-4 24-25 3.3330844630267606E-4 6.666168926053521E-5 0.0 0.0013332337852107043 2.3331591241187324E-4 26-27 3.3330844630267606E-4 6.666168926053521E-5 0.0 0.0015332188529923099 2.6664675704214083E-4 28-29 4.666318248237465E-4 6.666168926053521E-5 0.0 0.0017665347654041831 3.666392909329437E-4 30-31 5.332935140842817E-4 6.666168926053521E-5 0.0 0.00333308446302676 4.666318248237465E-4 32-33 5.666243587145493E-4 6.666168926053521E-5 0.0 0.006666168926053522 4.666318248237465E-4 34-35 7.999402711264226E-4 6.666168926053521E-5 0.0 0.010299230990752691 4.666318248237465E-4 36-37 9.33263649647493E-4 6.666168926053521E-5 0.0 0.01889858890536173 4.666318248237465E-4 38-39 0.0010332561835382959 6.666168926053521E-5 0.0 0.032664227737662255 4.666318248237465E-4 40-41 0.0010665870281685633 6.666168926053521E-5 0.0 0.050096259479292214 4.666318248237465E-4 42-43 0.0010665870281685633 6.666168926053521E-5 0.0 0.06826156980278805 4.666318248237465E-4 44-45 0.0010665870281685633 6.666168926053521E-5 0.0 0.08932666360911719 4.666318248237465E-4 46-47 0.0011999104066896338 6.666168926053521E-5 0.0 0.11372484187847308 4.666318248237465E-4 48-49 0.0013332337852107043 6.666168926053521E-5 0.0 0.14238936826050322 4.666318248237465E-4 50-51 0.0013332337852107043 6.666168926053521E-5 0.0 0.17515358853205626 4.666318248237465E-4 52-53 0.0013332337852107043 6.666168926053521E-5 0.0 0.21045095299550964 5.332935140842817E-4 54-55 0.0013332337852107043 6.666168926053521E-5 0.0 0.24474839212005503 5.332935140842817E-4 56-57 0.0013332337852107043 6.666168926053521E-5 0.0 0.287211888179016 5.332935140842817E-4 58-59 0.0013332337852107043 6.666168926053521E-5 0.0 0.3357415979606856 5.332935140842817E-4 60-61 0.0013332337852107043 6.666168926053521E-5 0.0 0.3943038919760658 5.332935140842817E-4 62-63 0.0013332337852107043 6.666168926053521E-5 0.0 0.45629926298836354 5.332935140842817E-4 64-65 0.0013332337852107043 6.666168926053521E-5 0.0 0.5168614076815599 5.332935140842817E-4 66-67 0.0013332337852107043 6.666168926053521E-5 0.0 0.5852563008628688 5.332935140842817E-4 68-69 0.0013332337852107043 6.666168926053521E-5 0.0 0.667716810478151 5.332935140842817E-4 70-71 0.0013665646298409717 6.666168926053521E-5 0.0 0.7660428021374404 5.332935140842817E-4 72-73 0.0014665571637317745 6.666168926053521E-5 0.0 0.8670685922117816 5.332935140842817E-4 74-75 0.0015998805422528452 6.666168926053521E-5 0.0 0.9635947182610365 5.332935140842817E-4 76-77 0.0015998805422528452 6.666168926053521E-5 0.0 1.0723532642895997 5.332935140842817E-4 78-79 0.0015998805422528452 6.666168926053521E-5 0.0 1.2085764262935035 5.332935140842817E-4 80-81 0.0019998506778160564 6.666168926053521E-5 0.0 1.35883187388675 5.332935140842817E-4 82-83 0.0020665123670765917 6.666168926053521E-5 0.0 1.5201864927418751 5.332935140842817E-4 84-85 0.0023331591241187322 6.666168926053521E-5 0.0 1.6843742333905736 5.332935140842817E-4 86-87 0.0024998133472700704 6.666168926053521E-5 0.0 1.8533949465106607 5.332935140842817E-4 88-89 0.0025331441919003383 6.666168926053521E-5 0.0 2.053213360069115 5.332935140842817E-4 90-91 0.0026664675704214085 6.666168926053521E-5 0.0 2.281362991563297 5.332935140842817E-4 92-93 0.0026664675704214085 6.666168926053521E-5 0.0 2.5227116375310645 5.332935140842817E-4 94-95 0.0026664675704214085 6.666168926053521E-5 0.0 2.7663934426229506 5.332935140842817E-4 96-97 0.0026664675704214085 6.666168926053521E-5 0.0 3.0179746578922106 5.332935140842817E-4 98-99 0.0027664601043122113 6.666168926053521E-5 0.0 3.2878211760188574 5.332935140842817E-4 100-101 0.0028331217935727467 6.666168926053521E-5 0.0 3.60326428959971 5.332935140842817E-4 102-103 0.002866452638203014 6.666168926053521E-5 0.0 3.949171795172627 5.332935140842817E-4 104-105 0.003666392909329437 1.3332337852107041E-4 0.0 4.298912347878025 5.332935140842817E-4 106-107 0.0037330545985899723 1.3332337852107041E-4 0.0 4.636853781584309 5.332935140842817E-4 108-109 0.003799716287850507 1.3332337852107041E-4 0.0 4.997426858794544 5.332935140842817E-4 110-111 0.0038330471324807746 1.3332337852107041E-4 0.0 5.410795993899122 5.332935140842817E-4 112-113 0.0038997088217413104 1.3332337852107041E-4 0.0 5.867295241955268 5.332935140842817E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2370 0.0 57.496136 1 GTATCAA 4685 0.0 55.376873 1 ATCAACG 5510 0.0 46.216843 3 TCAACGC 5580 0.0 45.317177 4 CAACGCA 5630 0.0 44.91471 5 AACGCAG 5840 0.0 43.499043 6 TATCAAC 5890 0.0 43.336124 2 GTACATG 7755 0.0 37.751553 1 ACGCAGA 6765 0.0 37.11157 7 CGCAGAG 6805 0.0 35.934143 8 TACATGG 8035 0.0 35.321617 2 ACATGGG 8110 0.0 33.60104 3 GCAGAGT 7665 0.0 30.893314 9 CATGGGG 3625 0.0 27.082207 4 CATGGGA 4460 0.0 26.414257 4 GAGTACT 3630 0.0 25.74007 12-13 AGAGTAC 7170 0.0 24.649635 10-11 CAGAGTA 7565 0.0 24.26719 10-11 GTATAGG 595 0.0 23.001831 1 GTACTTT 3935 0.0 22.91352 14-15 >>END_MODULE