FastQCFastQC Report
Thu 26 May 2016
SRR1364531_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364531_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1500112
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50690.337908102861653No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43120.2874452040914278No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28870.19245229689516516No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33100.066.4504851
GTATCAA60350.053.5330281
ATCAACG77900.041.5853963
TCAACGC79250.040.726724
TATCAAC79400.040.649782
CAACGCA79600.040.587255
AACGCAG83500.038.9765786
GTACATG73600.033.2861671
ACGCAGA98700.032.793267
CGCAGAG100000.032.3074538
TACATGG76550.031.1167472
ACATGGG76300.030.1260493
GCAGAGT108550.028.8299779
CATGGGG34650.028.1851274
GAGTACT67750.027.04856712-13
GTACTTT72800.025.17225814-15
AGAGTAC101400.024.9358910-11
CAGAGTA107350.024.49594110-11
GTGGTAT17350.024.3926331
TGGTATC16700.024.2477722
Produced by FastQC (version 0.11.5)