FastQCFastQC Report
Thu 26 May 2016
SRR1364530_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364530_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318837
Sequences flagged as poor quality0
Sequence length125
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36640.15801024392831406No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26430.11397955095593178No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA68350.054.1524541
GGTATCA44350.049.1081731
ATCAACG86750.042.2540363
TATCAAC89900.040.905882
CAACGCA89650.040.5553255
TCAACGC90150.040.39644
AACGCAG90250.039.815586
GTACATG129050.036.197451
TACATGG131950.034.2737122
ACGCAGA110100.032.745277
CGCAGAG110800.031.6262868
ACATGGG143500.030.851623
GCAGAGT117500.028.912149
CATGGGA87200.027.5008144
GAGTACT47050.025.4221412-13
CATGGGG56800.025.3526864
GAGTACA101400.023.8848651
AGTACAT96400.023.5799712
ATGGGAG38750.023.4950665
AGAGTAC115000.022.58523610-11
Produced by FastQC (version 0.11.5)