##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364530_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2318837 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.11951206574675 31.0 28.0 33.0 16.0 34.0 2 28.022345253245486 31.0 28.0 34.0 16.0 34.0 3 28.21628557764086 31.0 28.0 34.0 16.0 34.0 4 31.540489046879966 35.0 32.0 37.0 17.0 37.0 5 31.63955163730784 35.0 32.0 37.0 18.0 37.0 6 31.729227625745146 35.0 32.0 37.0 17.0 37.0 7 31.434161176486317 35.0 32.0 37.0 17.0 37.0 8 31.400891481376224 35.0 32.0 37.0 17.0 37.0 9 32.45647192967854 37.0 32.0 39.0 12.0 39.0 10-11 32.36694709459958 37.0 32.0 39.0 11.0 39.0 12-13 32.2888234489962 37.0 32.0 39.0 10.0 39.0 14-15 32.96610218829525 38.0 31.5 40.0 10.0 41.0 16-17 33.08408741105994 38.0 32.0 40.0 10.0 41.0 18-19 32.87889640367132 38.0 31.5 40.0 10.0 41.0 20-21 32.61150848464122 38.0 31.0 40.0 9.0 41.0 22-23 32.508268369014296 38.0 31.0 40.0 8.0 41.0 24-25 32.338861894993045 38.0 30.0 40.0 8.0 41.0 26-27 32.25807204214871 38.0 30.0 40.0 8.0 41.0 28-29 32.31748415261616 38.0 30.5 40.0 8.0 41.0 30-31 32.262885877705074 38.0 30.5 40.0 4.5 41.0 32-33 32.244724834043964 38.0 30.0 40.0 2.0 41.0 34-35 31.762301101802326 37.0 30.0 40.0 2.0 41.0 36-37 31.60638048297487 37.0 30.0 40.0 2.0 41.0 38-39 31.39213579910964 37.0 30.0 40.0 2.0 41.0 40-41 31.594350098777966 37.0 30.0 40.0 2.0 41.0 42-43 31.451994254016128 37.0 30.0 40.0 2.0 41.0 44-45 31.277913669654225 37.0 30.0 40.0 2.0 41.0 46-47 31.217874951969456 37.0 30.0 40.0 2.0 41.0 48-49 31.057218553956144 37.0 29.5 40.0 2.0 41.0 50-51 30.89057747482898 36.5 29.0 40.0 2.0 41.0 52-53 30.816663051348584 36.5 29.0 40.0 2.0 41.0 54-55 30.837655255630303 36.5 29.0 40.0 2.0 41.0 56-57 30.701045394738827 36.0 29.0 40.0 2.0 41.0 58-59 30.497315895856413 36.0 28.5 40.0 2.0 41.0 60-61 30.28651819856247 35.5 28.0 39.5 2.0 41.0 62-63 29.99255855413727 35.0 28.0 39.0 2.0 41.0 64-65 29.697404345367957 35.0 28.0 39.0 2.0 41.0 66-67 29.442897021222276 35.0 28.0 39.0 2.0 40.0 68-69 29.40042335877856 35.0 28.0 38.5 2.0 40.0 70-71 29.170086987571786 35.0 28.0 37.5 2.0 40.0 72-73 28.88902950056429 34.5 28.0 37.0 2.0 39.5 74-75 28.587669379089604 34.0 28.0 36.5 2.0 39.0 76-77 28.289138261982192 34.0 27.0 36.0 2.0 39.0 78-79 27.991845912412128 34.0 27.0 36.0 2.0 38.5 80-81 27.702866135049597 34.0 26.0 35.0 2.0 37.0 82-83 27.469410959028167 34.0 26.0 35.0 2.0 37.0 84-85 27.24176278884631 34.0 26.0 35.0 2.0 36.0 86-87 27.049370438715613 34.0 26.0 35.0 2.0 36.0 88-89 26.858977151045977 34.0 26.0 35.0 2.0 36.0 90-91 26.726458780845743 33.5 25.0 35.0 2.0 35.5 92-93 26.606815399271273 33.0 25.0 35.0 2.0 35.0 94-95 26.48542523687521 33.0 25.0 35.0 2.0 35.0 96-97 26.37438120920099 33.0 25.0 35.0 2.0 35.0 98-99 26.276482995570625 33.0 25.0 35.0 2.0 35.0 100-101 26.17845368173787 33.0 24.0 35.0 2.0 35.0 102-103 25.022028499631496 31.5 21.5 34.0 2.0 35.0 104-105 25.880299908962986 33.0 23.5 35.0 2.0 35.0 106-107 26.022530691031754 33.0 24.0 35.0 2.0 35.0 108-109 25.97088950193567 33.0 24.0 35.0 2.0 35.0 110-111 25.883155435246202 33.0 23.0 35.0 2.0 35.0 112-113 25.78298064935138 33.0 23.0 35.0 2.0 35.0 114-115 25.648527473039287 33.0 21.5 35.0 2.0 35.0 116-117 25.533186679356938 33.0 19.5 35.0 2.0 35.0 118-119 25.3749375225598 33.0 18.5 35.0 2.0 35.0 120-121 25.182950116804243 33.0 16.5 35.0 2.0 35.0 122-123 24.93346233478248 33.0 2.0 35.0 2.0 35.0 124-125 23.498006112546935 31.0 2.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7972535249571955 1101 2 0.7765658938497317 1101 3 0.7205739898455299 1101 4 0.8309398948531701 1101 5 0.6739180701947056 1101 6 0.9496834246785966 1101 7 0.9995994751639223 1101 8 0.764542634382412 1101 9 1.0741643262847944 1101 10-11 1.2294845975938244 1101 12-13 0.9675054353420265 1101 14-15 1.2448114345893089 1101 16-17 1.0228115030798648 1101 18-19 1.3294997784071825 1101 20-21 1.70094732740408 1101 22-23 1.5560536328864316 1101 24-25 1.7585608725443294 1101 26-27 1.8412655868895271 1101 28-29 1.6627750974310658 1101 30-31 1.7222294732669639 1101 32-33 1.7792160968237951 1101 34-35 2.0440067553074392 1101 36-37 1.9325619441041937 1101 38-39 1.6308529891694263 1101 40-41 1.5554695552322961 1101 42-43 1.6176218724634381 1101 44-45 1.5345577444904066 1101 46-47 1.4368720352605706 1101 48-49 1.4545022331037956 1101 50-51 1.368602679320908 1101 52-53 1.1845596948020614 1101 54-55 1.2331611678877081 1101 56-57 1.2182179665313129 1101 58-59 1.2272934559798294 1101 60-61 1.1107523896586144 1101 62-63 1.0855040772412679 1101 64-65 1.2515334724635814 1101 66-67 1.1223394189721851 1101 68-69 0.8682572898392458 1101 70-71 0.8643880384777702 1101 72-73 0.7235857643921122 1101 74-75 0.7277247042092583 1101 76-77 0.7554977628756987 1101 78-79 0.7965885700159916 1101 80-81 0.8404591991331394 1101 82-83 0.713515909117362 1101 84-85 0.8028424841952315 1101 86-87 0.8671657339614391 1101 88-89 0.9035915717965892 1101 90-91 0.7992849497922165 1101 92-93 0.7027450086552314 1101 94-95 0.8540455326758511 1101 96-97 0.7371432543102117 1101 98-99 0.8070037751983037 1101 100-101 0.8689620901666189 1101 102-103 0.7549774283954314 1101 104-105 0.8234580170025545 1101 106-107 0.7197111464264729 1101 108-109 0.8394602740606842 1101 110-111 0.5524056759962015 1101 112-113 0.7176559363403072 1101 114-115 0.8938611152351719 1101 116-117 0.9222923535161058 1101 118-119 0.9427596103227707 1101 120-121 0.9598255269717662 1101 122-123 1.0401015473802673 1101 124-125 1.3466554546780856 1102 1 -0.044625125483516825 1102 2 0.25004251640081065 1102 3 0.34786058534744413 1102 4 0.383879679869473 1102 5 0.40883165980804037 1102 6 -0.4563163140057007 1102 7 -0.8279342398809426 1102 8 0.17918091826301819 1102 9 0.3931128564005988 1102 10-11 0.5253447966858005 1102 12-13 0.5944345087418128 1102 14-15 0.8133933414359191 1102 16-17 0.5978458880371207 1102 18-19 0.9275469422341729 1102 20-21 1.136363617279656 1102 22-23 0.8851392839282326 1102 24-25 1.0218731787185575 1102 26-27 0.6784645335811277 1102 28-29 1.239889826327662 1102 30-31 0.9602175534809092 1102 32-33 0.7744424723550765 1102 34-35 1.2055678888856498 1102 36-37 1.344709791275072 1102 38-39 1.3698791842399274 1102 40-41 1.1615305667966815 1102 42-43 1.0176327107482521 1102 44-45 1.1263134668388162 1102 46-47 0.7973787383740216 1102 48-49 0.49352292591621705 1102 50-51 0.8399918099840775 1102 52-53 0.7690264212676858 1102 54-55 0.7068896725102718 1102 56-57 0.6739729129117258 1102 58-59 0.8271662557415205 1102 60-61 0.65600954292659 1102 62-63 0.5444999533736379 1102 64-65 0.6251708739881252 1102 66-67 0.5855783090653297 1102 68-69 0.5001897610080803 1102 70-71 0.5210895228573449 1102 72-73 0.7240237424735128 1102 74-75 0.3239583254331464 1102 76-77 0.6014567283314918 1102 78-79 0.4546155832772669 1102 80-81 0.05045142449232998 1102 82-83 0.3195113008515378 1102 84-85 -0.0850805496867082 1102 86-87 0.4827346391882834 1102 88-89 0.5697620101420426 1102 90-91 0.08014035809633668 1102 92-93 0.558131868930456 1102 94-95 0.4571772567344503 1102 96-97 0.6555433954738881 1102 98-99 0.7238314507918169 1102 100-101 0.6787392620126127 1102 102-103 0.5132407478495544 1102 104-105 0.7199550567544577 1102 106-107 0.7030970698217409 1102 108-109 0.23059165634760603 1102 110-111 0.5174350440909556 1102 112-113 0.21509235783122804 1102 114-115 0.25296195816116196 1102 116-117 0.2215414282723458 1102 118-119 0.5374706353851444 1102 120-121 0.9734723322044623 1102 122-123 0.8077481285330848 1102 124-125 1.1384367517215637 1103 1 0.3975229463281984 1103 2 0.3892636250588346 1103 3 0.09550167613425842 1103 4 0.2851654457325168 1103 5 0.2705365149092387 1103 6 0.3311349387205311 1103 7 0.45422682003985315 1103 8 0.3765677730105459 1103 9 0.6031539072652237 1103 10-11 0.6945128660995081 1103 12-13 0.5935265049451885 1103 14-15 0.8427892369523065 1103 16-17 0.6317128094868565 1103 18-19 0.7199102656628753 1103 20-21 1.1369877364460663 1103 22-23 1.0575620418550713 1103 24-25 1.2350494780653278 1103 26-27 1.2212671434609348 1103 28-29 1.1122962235239982 1103 30-31 1.0583686653407511 1103 32-33 0.9810227765809287 1103 34-35 1.374850081511564 1103 36-37 1.4213007425351663 1103 38-39 1.5422348330291342 1103 40-41 1.1829878239277498 1103 42-43 1.1940898037696357 1103 44-45 0.8781434572467788 1103 46-47 0.9542686824025779 1103 48-49 0.925694091979139 1103 50-51 0.9014887732851342 1103 52-53 0.8437736086286947 1103 54-55 0.7252795438616744 1103 56-57 0.7485538136260388 1103 58-59 0.7104671913676519 1103 60-61 0.6937517554198251 1103 62-63 0.5932919386586946 1103 64-65 0.8218430980561173 1103 66-67 0.8750324116064192 1103 68-69 0.908648836407437 1103 70-71 0.8565114588960938 1103 72-73 0.8912528057622424 1103 74-75 0.8214609696085233 1103 76-77 0.7729438936147268 1103 78-79 0.8196331932508478 1103 80-81 0.8097322874949064 1103 82-83 0.938463817222317 1103 84-85 0.8678447137595668 1103 86-87 0.8214845269908473 1103 88-89 0.8110480372409015 1103 90-91 0.8441680936372649 1103 92-93 0.9229853692225056 1103 94-95 0.8181977493448223 1103 96-97 0.8524441590192993 1103 98-99 0.8085646704094209 1103 100-101 0.7974068471352993 1103 102-103 0.730969876782158 1103 104-105 0.7876559939881957 1103 106-107 0.869664461295006 1103 108-109 0.9440258004720761 1103 110-111 0.9737034561924709 1103 112-113 1.063217429838538 1103 114-115 1.1002244495872695 1103 116-117 1.1228289943181196 1103 118-119 1.1105679762054486 1103 120-121 1.0858061209142527 1103 122-123 0.987506128712603 1103 124-125 1.1439487019206354 1104 1 0.8434041663306502 1104 2 0.9359997872511947 1104 3 0.9038942057314756 1104 4 0.8954325526791216 1104 5 0.9618223451062811 1104 6 0.9421958460040969 1104 7 0.8502436453109432 1104 8 0.9218564169413774 1104 9 1.2766086197199797 1104 10-11 1.1083690492547902 1104 12-13 1.152088369458582 1104 14-15 1.1666284618955416 1104 16-17 1.2731485278651746 1104 18-19 1.422483991585871 1104 20-21 1.7287867626810254 1104 22-23 1.5771134265559752 1104 24-25 2.0788726951595677 1104 26-27 2.046252572143324 1104 28-29 1.9746759655196584 1104 30-31 1.8737872892700125 1104 32-33 1.9667475547425823 1104 34-35 2.2483688473360637 1104 36-37 2.287354489135417 1104 38-39 2.2516127187955064 1104 40-41 1.886963472660387 1104 42-43 1.9613681453257144 1104 44-45 1.8224389992750716 1104 46-47 1.7088632481937864 1104 48-49 1.7222686100544422 1104 50-51 1.450786246253351 1104 52-53 1.593229347255786 1104 54-55 1.5086206687069676 1104 56-57 1.4997289951975645 1104 58-59 1.3909559636249647 1104 60-61 1.4374646644810696 1104 62-63 1.4311550611121433 1104 64-65 1.3764900711498562 1104 66-67 1.3488713344111183 1104 68-69 1.4770236112455564 1104 70-71 1.4138790521758793 1104 72-73 1.3379330448581968 1104 74-75 1.38908177146223 1104 76-77 1.3597147852585074 1104 78-79 1.3655186981270901 1104 80-81 1.3690544424348445 1104 82-83 1.2935529563307426 1104 84-85 1.2812380756111175 1104 86-87 1.3513064245636883 1104 88-89 1.4170451026516986 1104 90-91 1.4162061608955057 1104 92-93 1.4456449354504493 1104 94-95 1.3883924968586392 1104 96-97 1.2336556466560644 1104 98-99 1.2968043398808504 1104 100-101 1.3857337522423734 1104 102-103 1.311710083236168 1104 104-105 1.351241323679023 1104 106-107 1.3894141587444615 1104 108-109 1.4310536197404922 1104 110-111 1.5420644974607463 1104 112-113 1.4906596842974125 1104 114-115 1.6200436389001318 1104 116-117 1.5993127280033264 1104 118-119 1.438780244458325 1104 120-121 1.393736378550532 1104 122-123 1.4504738323004176 1104 124-125 1.6824005001118678 1105 1 0.24650415834602057 1105 2 0.4195105943204105 1105 3 0.421072063334865 1105 4 0.4089906335712641 1105 5 0.4235161777513774 1105 6 0.30920569658598396 1105 7 0.5510392140195428 1105 8 0.24484022598874944 1105 9 0.1800765332615626 1105 10-11 0.4724467790497755 1105 12-13 0.47469785530812914 1105 14-15 0.5410795767524732 1105 16-17 0.48704820195413845 1105 18-19 0.5936661258162701 1105 20-21 1.0019844612762014 1105 22-23 1.1685182008373047 1105 24-25 1.1663111877047996 1105 26-27 0.9762341919741999 1105 28-29 0.8754563409276486 1105 30-31 0.7770472066814591 1105 32-33 0.8305104774866621 1105 34-35 1.3932540397575046 1105 36-37 1.2701735664892624 1105 38-39 1.4518123500747393 1105 40-41 1.0489579278040289 1105 42-43 0.6446517667691936 1105 44-45 0.7345046681655631 1105 46-47 0.670497525893957 1105 48-49 0.4989913406211173 1105 50-51 0.4088454037514637 1105 52-53 0.40048552950525007 1105 54-55 0.34956663712430114 1105 56-57 0.3465918540878832 1105 58-59 0.37005190636965324 1105 60-61 0.3448010499762333 1105 62-63 0.35170096618008984 1105 64-65 0.3670169939035617 1105 66-67 0.11484100259269425 1105 68-69 -0.020722263187476386 1105 70-71 0.22118353794744294 1105 72-73 0.3069679265192278 1105 74-75 0.33166444167937925 1105 76-77 0.2884834241133625 1105 78-79 0.34478201548572684 1105 80-81 0.32656565564162676 1105 82-83 0.33048194953247645 1105 84-85 0.23853233034752108 1105 86-87 0.2351782781517926 1105 88-89 0.4043351074541164 1105 90-91 0.3686316145936104 1105 92-93 0.36321712826507024 1105 94-95 0.39364202276635396 1105 96-97 0.4239248109523679 1105 98-99 0.3288111459829075 1105 100-101 0.33966224083719254 1105 102-103 0.2824916124969654 1105 104-105 0.3469910017137394 1105 106-107 0.3151165132947966 1105 108-109 0.34867923741450824 1105 110-111 0.3898384112154112 1105 112-113 0.42549396519454064 1105 114-115 0.4016217463937437 1105 116-117 0.470936282709566 1105 118-119 0.46016023689892194 1105 120-121 0.3307985963580542 1105 122-123 0.5550783102580645 1105 124-125 0.7278602444997198 1106 1 0.255145855447946 1106 2 0.46959099809776106 1106 3 0.2518872097627707 1106 4 0.44461493633387406 1106 5 0.25337304755603185 1106 6 0.1590324509212877 1106 7 -0.1090167896903651 1106 8 0.23568686134249361 1106 9 0.29404882872693605 1106 10-11 0.4477291587187153 1106 12-13 0.3211713879057996 1106 14-15 0.7169073867949507 1106 16-17 0.45396039361197893 1106 18-19 0.4428541993109718 1106 20-21 0.8170201012150002 1106 22-23 0.8087343190878258 1106 24-25 1.2037597204085415 1106 26-27 1.4192959345007665 1106 28-29 1.2958825304486155 1106 30-31 1.0947624618072922 1106 32-33 0.9607713933621369 1106 34-35 1.303161736881119 1106 36-37 1.2792492224129575 1106 38-39 1.3087904676441795 1106 40-41 1.0617135906798616 1106 42-43 0.8889234003196833 1106 44-45 0.6055925368518089 1106 46-47 0.36274320897405943 1106 48-49 0.4190776232322655 1106 50-51 0.4674858279703713 1106 52-53 0.3612149276185832 1106 54-55 0.140728452456635 1106 56-57 0.16582097570274357 1106 58-59 0.140971542350254 1106 60-61 0.10007161069063386 1106 62-63 0.15880194839005668 1106 64-65 0.14226003205846283 1106 66-67 0.006833578384625838 1106 68-69 0.08117964404700828 1106 70-71 0.24593965906116466 1106 72-73 0.09683591912141765 1106 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-2.2744289777010565 2216 72-73 -2.24480202679117 2216 74-75 -2.151327577258911 2216 76-77 -2.211691979029542 2216 78-79 -2.49221980240819 2216 80-81 -2.40583858780219 2216 82-83 -2.196922057707603 2216 84-85 -2.2454363254170318 2216 86-87 -2.2074381024896503 2216 88-89 -2.2830450956985935 2216 90-91 -2.247346496318915 2216 92-93 -2.131968471005674 2216 94-95 -2.255601666001109 2216 96-97 -2.238001706066111 2216 98-99 -2.3231309517337024 2216 100-101 -2.2851732913779337 2216 102-103 -2.2046855866691466 2216 104-105 -2.4323035102237363 2216 106-107 -2.3502386758825224 2216 108-109 -2.39347508060845 2216 110-111 -2.369705641218321 2216 112-113 -2.4134369592106424 2216 114-115 -2.611546830461265 2216 116-117 -2.572693280724696 2216 118-119 -2.6869706313422625 2216 120-121 -2.703128680011684 2216 122-123 -2.82351592322145 2216 124-125 -2.869977310319232 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 156730.0 3 27005.0 4 17074.0 5 13597.0 6 12668.0 7 13171.0 8 13992.0 9 14624.0 10 15863.0 11 16808.0 12 17792.0 13 19805.0 14 21925.0 15 19398.0 16 14652.0 17 13138.0 18 12209.0 19 11507.0 20 11458.0 21 11977.0 22 14520.0 23 19508.0 24 25964.0 25 33292.0 26 40275.0 27 45461.0 28 52208.0 29 61206.0 30 68692.0 31 78645.0 32 96518.0 33 120121.0 34 155004.0 35 212506.0 36 320013.0 37 396860.0 38 122358.0 39 293.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.6556236435593 18.99288698886554 13.06987761411354 23.281611753461622 2 15.183184882368442 20.664650326925642 40.4117697543001 23.740395036405815 3 19.1297604768984 26.335853335282742 28.337838111762498 26.19654807605636 4 12.171613728511732 16.590825088230257 37.57287109864408 33.66469008461393 5 13.457577027985238 38.879960733368186 33.415023799788486 14.247438438858092 6 31.23552874499584 36.823929057050975 17.859981430218202 14.080560767734982 7 28.487290869506715 32.88355972963555 20.856248986478327 17.772900414379414 8 26.170921026359334 35.41853092735712 19.733297338277765 18.67725070800578 9 27.20276077164982 16.46229124726364 19.544871164564693 36.79007681652185 10-11 25.001929846729197 27.353669102226675 26.895594645074233 20.748806405969887 12-13 25.689583418688045 24.658987258248498 27.404232012437706 22.247197310625747 14-15 22.854848185009235 25.8971219117897 25.29703495579306 25.950994947408013 16-17 22.421403181833867 29.268449977568416 25.691582979604515 22.618563860993202 18-19 22.339354452787006 27.816541125747428 28.03375340357817 21.8103510178874 20-21 23.573829139616954 26.924058098525112 27.847592184668002 21.654520577189935 22-23 23.23172665796818 26.678603903881953 27.54571394748435 22.543955490665514 24-25 22.70212418166754 26.974920501150052 27.796951332859194 22.52600398432321 26-27 22.459757998042626 27.2240340474575 27.629829223550097 22.686378730949773 28-29 22.564081554323366 27.092349673163856 27.861813485077015 22.481755287435757 30-31 22.556092663566496 27.06250266840953 27.625962945441106 22.755441722582873 32-33 22.507859136329543 27.18161386917066 27.30086486080843 23.009662133691357 34-35 22.851050597485674 26.94516553503724 27.734747154896873 22.469036712580216 36-37 23.06303361043535 26.73898965336765 27.45850316605354 22.739473570143456 38-39 22.742693859033643 26.86062021608246 27.66841740061936 22.728268524264532 40-41 22.931581633115226 26.849764976721975 27.391739827915597 22.8269135622472 42-43 22.67461418961057 26.948783460549482 27.262935031350466 23.113667318489483 44-45 22.837645899837693 26.977360373731322 27.22392522268043 22.961068503750553 46-47 23.035735310036515 27.383414203298944 26.703024009129084 22.877826477535457 48-49 22.819237340738812 27.253488896739526 26.730321535377694 23.196952227143967 50-51 22.99061599589086 27.479382059081402 26.515148574674186 23.01485337035355 52-53 23.224120539736084 27.265909591747935 26.530778144388762 22.979191724127222 54-55 22.99380233284867 27.171221499853566 26.67142702483078 23.163549142466984 56-57 22.79035797274708 27.427110772994112 26.48165542772663 23.30087582653218 58-59 22.904810379547094 26.997263058797554 26.615734360805742 23.48219220084961 60-61 23.129970923788584 27.105976795958487 26.71123552766294 23.05281675258999 62-63 22.896011629512046 27.187823521292014 26.735368210366484 23.180796638829456 64-65 22.825416529361934 27.046717539867256 26.67903559456256 23.44883033620825 66-67 22.774936734190447 27.409275880330586 26.542271220999936 23.27351616447903 68-69 22.95404515277629 27.0441540157356 26.84507047721172 23.15673035427639 70-71 22.72835382808388 26.99694169028377 26.900381647209105 23.374322834423246 72-73 22.98747310574636 27.186243771218617 26.452602389014096 23.373680734020926 74-75 22.917058386190313 27.34986432848205 26.56746747074826 23.165609814579383 76-77 23.086339860218576 27.224018374156284 26.635755554371936 23.0538862112532 78-79 23.070732893797157 27.290400930133067 26.492340029877422 23.146526146192354 80-81 22.72079491019417 27.463905699293043 26.703518378955927 23.11178101155686 82-83 23.09225763645722 27.43684272150896 26.323505597412794 23.14739404462103 84-85 22.967418580952433 27.355976293288403 26.433293931397507 23.243311194361656 86-87 22.96780554158105 27.26498588760587 26.58033692491913 23.18687164589395 88-89 22.950492645205088 27.321811716301404 26.48532739604223 23.24236824245127 90-91 22.888515681158864 27.57072465012135 26.449383276263035 23.091376392456755 92-93 22.949963492899336 27.414589988385867 26.41908685239789 23.216359666316905 94-95 22.814344097797886 27.248967612571583 26.574225027353116 23.362463262277412 96-97 22.91748483093788 27.287835312563015 26.517838946452425 23.27684091004668 98-99 22.732686939395286 27.225050057939125 26.499053610618883 23.54320939204671 100-101 22.66852177153314 27.217510901831975 26.658305468663407 23.45566185797148 102-103 22.831984309375777 27.092417448919438 26.549688486081603 23.525909755623182 104-105 22.7538332857872 27.079927747126153 26.707687875692628 23.45855109139402 106-107 22.65507349377015 27.194549334234935 26.755212529044748 23.39516464295017 108-109 22.78480548654347 27.164802873164433 26.360089130887598 23.6903025094045 110-111 22.577386409800166 27.20433427720502 26.682840136655834 23.53543917633898 112-113 22.858441537719123 27.24372174499544 26.574205086429103 23.32363163085633 114-115 22.590785812025597 27.578954450010933 26.414965778103422 23.41529395986005 116-117 22.45733529351136 27.545920649015006 26.538540656372138 23.458203401101503 118-119 22.49394847503296 27.614187629402153 26.396702312409193 23.495161583155692 120-121 22.531822000348452 27.5186990369306 26.525140199651894 23.42433876306906 122-123 22.58341142564139 27.549456904474095 26.480623691962823 23.386507977921692 124-125 22.557985200701122 27.560453619711634 26.237126531654535 23.64443464793271 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1342.0 1 1332.5 2 1563.0 3 1623.0 4 1248.0 5 956.5 6 841.0 7 953.0 8 1238.5 9 1407.0 10 1337.0 11 1263.0 12 1329.0 13 1455.0 14 1552.5 15 1643.0 16 1813.0 17 2017.0 18 2209.0 19 2441.5 20 2789.0 21 3311.5 22 4205.5 23 5382.5 24 6695.5 25 8379.5 26 10887.5 27 14200.0 28 17499.0 29 21059.0 30 25286.5 31 30336.5 32 35189.0 33 38605.0 34 42422.5 35 46673.0 36 51535.0 37 56441.0 38 59548.5 39 61759.5 40 63343.5 41 66396.0 42 70707.5 43 74694.5 44 78432.0 45 80795.0 46 81568.0 47 82178.0 48 85177.5 49 87056.5 50 85493.5 51 83892.5 52 83079.5 53 83835.5 54 82035.5 55 76434.5 56 71043.5 57 66198.0 58 63155.5 59 58712.5 60 50357.0 61 41183.5 62 32837.0 63 24241.0 64 17102.0 65 12926.5 66 10014.5 67 7820.5 68 6044.5 69 4678.5 70 3629.5 71 2833.5 72 2283.5 73 1934.5 74 1557.0 75 1211.0 76 897.5 77 572.5 78 362.5 79 252.5 80 184.5 81 142.0 82 107.5 83 72.5 84 56.5 85 44.5 86 28.0 87 23.0 88 19.0 89 16.0 90 15.5 91 14.0 92 10.0 93 8.5 94 7.5 95 6.0 96 5.0 97 2.0 98 1.5 99 3.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011600642908492489 2 0.004916257589472653 3 0.008107512515972446 4 0.008107512515972446 5 0.014188146902951781 6 6.900010651891443E-4 7 0.008150637582546768 8 0.0 9 0.002803129327330899 10-11 0.0 12-13 0.003536255459094365 14-15 0.018241903160938006 16-17 0.02919567007081567 18-19 0.0059296966539692094 20-21 0.00817220011583393 22-23 0.0018328153294086649 24-25 0.010781266643580381 26-27 0.018371278360660968 28-29 6.253134653276621E-4 30-31 0.001725002662972861 32-33 0.008538763181715661 34-35 0.008797513581161591 36-37 0.011061579576313471 38-39 0.0 40-41 0.023977537015322768 42-43 0.009099389047181843 44-45 0.016387525298242177 46-47 0.004053756257986223 48-49 0.023891286882174127 50-51 0.004485006923729438 52-53 0.0 54-55 0.016560025564539466 56-57 0.004873132522898332 58-59 3.2343799930741144E-4 60-61 0.004441881857155118 62-63 0.025745664744869952 64-65 0.016948151163708357 66-67 0.0021131282621417546 68-69 0.019988468357198027 70-71 0.003126567326638311 72-73 0.011449705175482366 74-75 4.312506657432152E-5 76-77 0.006274697186563783 78-79 0.0012506269306553242 80-81 0.0036440681255301686 82-83 0.0018328153294086649 84-85 0.0 86-87 0.005627821187948958 88-89 0.016538463031252303 90-91 0.021454720620724958 92-93 0.004743757323175368 94-95 0.023524723816292392 96-97 0.016581588097826627 98-99 0.0010350015977837166 100-101 0.008193762649121089 102-103 0.0 104-105 0.0015740649299627355 106-107 4.312506657432152E-5 108-109 0.0 110-111 8.193762649121089E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 4.312506657432152E-5 122-123 0.0 124-125 0.023783474215738322 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2318837.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.188265266089736 #Duplication Level Percentage of deduplicated Percentage of total 1 75.57791086678243 38.68702149705746 2 11.575581148895688 11.850678369176407 3 4.80836658213651 7.383958323090149 4 2.460297474547489 5.037534390425102 5 1.4433534738766238 3.6941380246764375 6 0.8899085679808837 2.7331725504222915 7 0.6354963903489153 2.277097046337595 8 0.46811299312972277 1.9169513653461996 9 0.3252594637800704 1.4984520941052317 >10 1.6475420610561737 15.032012443883469 >50 0.11003254594136988 3.869514968497344 >100 0.05641224291810737 5.068443786239478 >500 0.0012083320243394047 0.3788018933721612 >1k 5.178565818596844E-4 0.5722232473706257 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3664 0.15801024392831406 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2643 0.11397955095593178 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 8.625013314864305E-5 0.0 0.0 0.0 0.0 7 8.625013314864305E-5 0.0 0.0 0.0 0.0 8 1.725002662972861E-4 0.0 0.0 0.0 0.0 9 1.725002662972861E-4 0.0 0.0 0.0 0.0 10-11 2.156253328716076E-4 0.0 0.0 0.0 0.0 12-13 3.0187546602025066E-4 0.0 0.0 0.0 0.0 14-15 5.175007988918583E-4 0.0 0.0 0.0 0.0 16-17 5.175007988918583E-4 0.0 0.0 4.312506657432153E-5 0.0 18-19 6.468759986148229E-4 0.0 0.0 6.468759986148229E-5 0.0 20-21 7.331261317634659E-4 0.0 0.0 1.9406279958444688E-4 0.0 22-23 7.762511983377874E-4 0.0 0.0 3.2343799930741144E-4 0.0 24-25 9.05626398060752E-4 0.0 0.0 6.253134653276621E-4 0.0 26-27 9.05626398060752E-4 0.0 0.0 0.0010781266643580382 0.0 28-29 0.0010996891976451989 0.0 0.0 0.001962190529131629 0.0 30-31 0.001164376797506681 0.0 0.0 0.004269381590857831 0.0 32-33 0.001164376797506681 0.0 0.0 0.009228764246904806 0.0 34-35 0.0013153145305168064 0.0 0.0 0.015632836633191552 0.0 36-37 0.0015525023966755749 0.0 0.0 0.026629728609643542 0.0 38-39 0.0017250026629728609 0.0 0.0 0.046057571101375386 0.0 40-41 0.0017681277295471824 0.0 0.0 0.0684179181201611 0.0 42-43 0.0019190654625573077 0.0 0.0 0.09192107940316632 0.0 44-45 0.0021131282621417546 0.0 0.0 0.11988768507661383 0.0 46-47 0.0023071910617262015 0.0 0.0 0.15346054940472315 0.0 48-49 0.002630629061033613 0.0 0.0 0.1904187314589167 0.0 50-51 0.002630629061033613 0.0 0.0 0.2346434872308834 0.0 52-53 0.002652191594320774 0.0 0.0 0.2780919918045124 0.0 54-55 0.0026737541276079346 0.0 0.0 0.3235026869072729 0.0 56-57 0.002803129327330899 0.0 0.0 0.37906933518828617 0.0 58-59 0.002803129327330899 0.0 0.0 0.44556818784589003 0.0 60-61 0.0028678169271923815 0.0 0.0 0.5193767392878412 0.0 62-63 0.002889379460479542 0.0 0.0 0.5970881092547686 0.0 64-65 0.002889379460479542 0.0 0.0 0.6746485414886859 0.0 66-67 0.0029325045270538635 0.0 0.0 0.7632705532989166 0.0 68-69 0.0029756295936281853 0.0 0.0 0.869142591738876 0.0 70-71 0.0031481298599254715 0.0 0.0 0.9907121544118884 0.0 72-73 0.0032990675929355966 0.0 0.0 1.1147182833463498 0.0 74-75 0.003363755192797079 0.0 0.0 1.237732535749602 0.0 76-77 0.0034068802593714004 0.0 0.0 1.375193685455252 0.0 78-79 0.003428442792658561 0.0 0.0 1.5392845637705452 0.0 80-81 0.0037303182586788117 0.0 0.0 1.7291857944305702 0.0 82-83 0.0038381309251146155 0.0 0.0 1.9316579819970097 0.0 84-85 0.003924381058263258 0.0 0.0 2.128847348908095 0.0 86-87 0.004032193724699063 0.0 0.0 2.3355242304655306 0.0 88-89 0.004075318791273383 0.0 0.0 2.571181156760911 0.0 90-91 0.004290944124144992 0.0 0.0 2.8387506323212888 0.0 92-93 0.004312506657432153 0.0 0.0 3.1329929615578846 0.0 94-95 0.004312506657432153 0.0 0.0 3.4298227947889393 0.0 96-97 0.004312506657432153 0.0 0.0 3.7276876296177783 0.0 98-99 0.004635944656739564 0.0 0.0 4.05407969598553 0.0 100-101 0.004851569989611172 0.0 0.0 4.431639653843716 0.0 102-103 0.0049593826560469755 0.0 0.0 4.847322170553601 0.0 104-105 0.006145321986840818 0.0 0.0 5.264169064060993 0.0 106-107 0.006468759986148228 0.0 0.0 5.682697835164783 0.0 108-109 0.006684385319019836 0.0 0.0 6.117182880901073 0.0 110-111 0.0067921979854556405 0.0 0.0 6.609347703180517 0.0 112-113 0.006900010651891443 0.0 0.0 7.148971661225002 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6835 0.0 54.152454 1 GGTATCA 4435 0.0 49.108173 1 ATCAACG 8675 0.0 42.254036 3 TATCAAC 8990 0.0 40.90588 2 CAACGCA 8965 0.0 40.555325 5 TCAACGC 9015 0.0 40.3964 4 AACGCAG 9025 0.0 39.81558 6 GTACATG 12905 0.0 36.19745 1 TACATGG 13195 0.0 34.273712 2 ACGCAGA 11010 0.0 32.74527 7 CGCAGAG 11080 0.0 31.626286 8 ACATGGG 14350 0.0 30.85162 3 GCAGAGT 11750 0.0 28.91214 9 CATGGGA 8720 0.0 27.500814 4 GAGTACT 4705 0.0 25.42214 12-13 CATGGGG 5680 0.0 25.352686 4 GAGTACA 10140 0.0 23.884865 1 AGTACAT 9640 0.0 23.579971 2 ATGGGAG 3875 0.0 23.495066 5 AGAGTAC 11500 0.0 22.585236 10-11 >>END_MODULE