FastQCFastQC Report
Thu 26 May 2016
SRR1364530_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364530_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2318837
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84920.3662180653491384No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69470.2995898374918116No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45020.1941490497175955No Hit
ATCCAATACAGTGGCCTCGTGTGGCTGTATAACTCGGTTCACTTCAGACT23470.10121453124993263No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA59800.059.521111
GTATCAA95900.054.336381
ATCAACG130000.040.217443
TATCAAC130400.039.5917632
CAACGCA131500.039.4981775
TCAACGC132800.039.2798084
AACGCAG134650.038.8392756
GTACATG124650.033.290081
ACGCAGA159200.032.700447
CGCAGAG162150.031.9220548
TACATGG129750.031.2997112
GTGGTAT24150.031.106511
ACATGGG134600.029.021653
GCAGAGT173200.028.957989
TATCACG1454.0440573E-628.7470532
TGGTATC26750.026.9354922
ACCGTAT2057.781455E-826.1202438
GAGTACT102450.026.01684412-13
CATGGGG55100.025.6129764
AGTACTT105850.025.49030112-13
Produced by FastQC (version 0.11.5)