##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364530_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2318837 Sequences flagged as poor quality 0 Sequence length 125 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.018274678211533 31.0 30.0 34.0 26.0 34.0 2 29.92513272817365 31.0 30.0 34.0 26.0 34.0 3 30.168983848368814 31.0 30.0 34.0 26.0 34.0 4 33.74057339951018 35.0 35.0 37.0 28.0 37.0 5 33.13876697672152 35.0 33.0 37.0 26.0 37.0 6 33.17007448130248 35.0 33.0 37.0 26.0 37.0 7 32.95267023943468 35.0 33.0 37.0 26.0 37.0 8 32.88162169225349 35.0 33.0 37.0 25.0 37.0 9 34.103275046930854 38.0 34.0 39.0 25.0 39.0 10-11 34.03000944007707 38.0 34.0 39.0 25.0 39.0 12-13 33.91410219864527 38.0 33.5 39.0 24.0 39.0 14-15 34.8572987234549 39.0 34.0 41.0 24.5 41.0 16-17 34.87048421256 39.0 34.0 41.0 24.0 41.0 18-19 34.89941509472205 39.0 34.0 41.0 24.0 41.0 20-21 34.91606848605572 39.0 34.0 41.0 24.0 41.0 22-23 34.867234091917624 39.0 34.0 41.0 24.0 41.0 24-25 34.82297440484174 39.0 34.0 41.0 24.0 41.0 26-27 34.692109234068624 39.0 34.0 41.0 24.0 41.0 28-29 34.53130685770496 39.0 33.0 41.0 23.0 41.0 30-31 34.359600739508636 38.0 33.0 40.5 22.5 41.0 32-33 34.12934803093103 38.0 33.0 40.0 21.5 41.0 34-35 33.92389439188696 38.0 33.0 40.0 19.0 41.0 36-37 33.702592506502185 38.0 33.0 40.0 17.5 41.0 38-39 33.51901125434863 38.0 33.0 40.0 16.0 41.0 40-41 33.52721601388973 38.0 33.0 40.0 15.5 41.0 42-43 33.37156061422169 38.0 32.5 40.0 14.5 41.0 44-45 33.1824709973146 38.0 32.0 40.0 10.5 41.0 46-47 32.89441129324743 38.0 32.0 40.0 7.5 41.0 48-49 32.58833113323619 38.0 31.0 40.0 3.5 41.0 50-51 32.40120349123289 38.0 31.0 40.0 2.0 41.0 52-53 32.21019890574456 37.5 31.0 40.0 2.0 41.0 54-55 32.00807236558671 37.0 31.0 40.0 2.0 41.0 56-57 31.787028583725377 37.0 30.0 40.0 2.0 41.0 58-59 31.579384191299347 36.5 30.0 40.0 2.0 41.0 60-61 31.329976837526743 36.0 30.0 40.0 2.0 41.0 62-63 31.150555213669612 36.0 30.0 39.5 2.0 41.0 64-65 30.998787754378597 35.5 30.0 39.0 2.0 41.0 66-67 30.698219193500883 35.0 29.5 39.0 2.0 41.0 68-69 30.40665579339988 35.0 29.0 39.0 2.0 40.5 70-71 30.086451311584213 35.0 29.0 38.0 2.0 40.0 72-73 29.80451795447459 35.0 29.0 37.0 2.0 39.5 74-75 29.45648400469718 35.0 29.0 37.0 2.0 39.0 76-77 29.154445741550614 34.5 29.0 36.0 2.0 39.0 78-79 28.880948725589597 34.0 29.0 36.0 2.0 38.5 80-81 28.61237335785137 34.0 28.5 35.0 2.0 37.0 82-83 28.338345472320825 34.0 28.0 35.0 2.0 37.0 84-85 28.124983558568367 34.0 28.0 35.0 2.0 36.5 86-87 27.919697891658622 34.0 27.0 35.0 2.0 36.0 88-89 27.75201598904968 34.0 27.0 35.0 2.0 36.0 90-91 27.612030513572105 34.0 27.0 35.0 2.0 35.5 92-93 27.510341606589854 34.0 27.0 35.0 2.0 35.0 94-95 27.399570129336386 34.0 27.0 35.0 2.0 35.0 96-97 27.286064307236774 34.0 26.5 35.0 2.0 35.0 98-99 27.201326138922227 34.0 26.0 35.0 2.0 35.0 100-101 27.100094142020332 34.0 26.0 35.0 2.0 35.0 102-103 25.832234003511243 31.5 24.0 34.0 2.0 35.0 104-105 26.76771157265474 33.5 25.0 35.0 2.0 35.0 106-107 26.928783049433832 34.0 25.0 35.0 2.0 35.0 108-109 26.92928222207943 34.0 25.0 35.0 2.0 35.0 110-111 26.84874486649989 34.0 25.0 35.0 2.0 35.0 112-113 26.762221536054497 34.0 25.0 35.0 2.0 35.0 114-115 26.658291634987712 34.0 25.0 35.0 2.0 35.0 116-117 26.53538109836957 34.0 25.0 35.0 2.0 35.0 118-119 26.399485172955238 34.0 25.0 35.0 2.0 35.0 120-121 26.199464429798212 34.0 24.5 35.0 2.0 35.0 122-123 25.900295061705503 34.0 23.5 35.0 2.0 35.0 124-125 24.10346026909179 31.5 11.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5959002178971851 1101 2 0.7660663091853444 1101 3 0.5808175619525038 1101 4 0.49075566780529556 1101 5 0.6808819124629792 1101 6 0.6790989886876275 1101 7 0.7856727711729619 1101 8 0.8235300565091208 1101 9 1.0001329848474754 1101 10-11 1.0344003800099557 1101 12-13 0.995900576456016 1101 14-15 1.1610298424539707 1101 16-17 1.081734523377989 1101 18-19 1.0708225523264758 1101 20-21 1.118585544242002 1101 22-23 1.1033880817832937 1101 24-25 1.131580718482212 1101 26-27 1.1382051717128974 1101 28-29 1.2014870989710786 1101 30-31 1.3547329352267283 1101 32-33 1.2739491072315872 1101 34-35 1.3161317010097306 1101 36-37 1.353829066420495 1101 38-39 1.2676901635284068 1101 40-41 1.02599545991486 1101 42-43 1.0740577001115383 1101 44-45 1.22724761563709 1101 46-47 1.238276064066227 1101 48-49 1.2600915791862235 1101 50-51 1.2145934892318664 1101 52-53 1.2296145514635484 1101 54-55 1.3051306836356034 1101 56-57 1.323368299939922 1101 58-59 1.3788795056537566 1101 60-61 1.3106010509353858 1101 62-63 1.2739443470875784 1101 64-65 1.267908815206102 1101 66-67 1.3388147414014284 1101 68-69 1.325883551543157 1101 70-71 1.2835347017920036 1101 72-73 1.10167562496623 1101 74-75 1.2243959240638311 1101 76-77 1.1952356241981654 1101 78-79 1.1634915185110621 1101 80-81 1.0115272522346466 1101 82-83 1.1341898113810345 1101 84-85 1.1283473812686324 1101 86-87 1.188583984741662 1101 88-89 1.1753635324676175 1101 90-91 1.1169342662124357 1101 92-93 1.1090798076166628 1101 94-95 1.0530689871649237 1101 96-97 1.149453109549441 1101 98-99 1.119725024180255 1101 100-101 1.0665571779087912 1101 102-103 0.9821653598536173 1101 104-105 1.10559110035231 1101 106-107 1.1525145351125161 1101 108-109 1.1252764630214145 1101 110-111 1.1235245140237353 1101 112-113 1.049753880559404 1101 114-115 1.1452821022702473 1101 116-117 1.0909733317937302 1101 118-119 1.070660989514714 1101 120-121 1.1367214358218867 1101 122-123 1.1083826219802866 1101 124-125 1.4495878843492882 1102 1 0.4569944657601148 1102 2 -0.2717557308325276 1102 3 0.2829770070855169 1102 4 0.4232234288949215 1102 5 0.5325930648585668 1102 6 0.32043529268621995 1102 7 0.47636034065767063 1102 8 0.5697748900383814 1102 9 0.9066433031605854 1102 10-11 0.9454576663295171 1102 12-13 0.9296504384839821 1102 14-15 1.1912398990524267 1102 16-17 1.1852538989730945 1102 18-19 1.1666089696283635 1102 20-21 0.9268564521578 1102 22-23 1.1430867907640447 1102 24-25 1.2364661418535476 1102 26-27 1.3030770937729983 1102 28-29 0.9465108800297415 1102 30-31 1.1519467147427633 1102 32-33 1.2361789729565444 1102 34-35 1.3115920056253785 1102 36-37 1.036367434281189 1102 38-39 0.4125227286512043 1102 40-41 0.07440531563933916 1102 42-43 0.3271063417353375 1102 44-45 0.07129379836657534 1102 46-47 0.2769114325812154 1102 48-49 -0.1444467364778248 1102 50-51 -0.16165170834408116 1102 52-53 -0.3608006811805673 1102 54-55 -0.27848227855407615 1102 56-57 -0.29185742063978637 1102 58-59 -0.17660253869942721 1102 60-61 -0.3588981288790727 1102 62-63 -0.23394453622331923 1102 64-65 -0.2665777509725977 1102 66-67 -0.3774783590661457 1102 68-69 -0.21276017009777348 1102 70-71 -0.6651594012501683 1102 72-73 -0.4170787219838914 1102 74-75 -0.31370235092007803 1102 76-77 -0.6078833997843631 1102 78-79 -0.4357042624704732 1102 80-81 -0.361232489255638 1102 82-83 -0.6767390528996984 1102 84-85 -0.5123072079138282 1102 86-87 -0.311810025144446 1102 88-89 -0.4763246633768645 1102 90-91 -0.44086126156548744 1102 92-93 -0.3173670535362625 1102 94-95 -0.09666773560372022 1102 96-97 -0.45804559550824564 1102 98-99 -0.5681351991814978 1102 100-101 -0.36665268153926434 1102 102-103 -0.41021737056964724 1102 104-105 -0.3365997824559095 1102 106-107 -0.28541662748303054 1102 108-109 -0.23596045290807055 1102 110-111 -0.1534460673471827 1102 112-113 0.04145997453464645 1102 114-115 -0.0491502442250642 1102 116-117 -0.07135778545390536 1102 118-119 0.2093349735751211 1102 120-121 0.3309245492688113 1102 122-123 0.22980848146292132 1102 124-125 0.502376024805482 1103 1 0.2704457995364322 1103 2 -0.019866208800301877 1103 3 0.2192008031233037 1103 4 0.2977316859563288 1103 5 0.18608133336076094 1103 6 0.36963545893937777 1103 7 0.6072467707295317 1103 8 0.5445840539230034 1103 9 0.7965928578539874 1103 10-11 0.7328474197898842 1103 12-13 0.6581991680071084 1103 14-15 0.6956090681349636 1103 16-17 0.6909420996209548 1103 18-19 0.7840621849724201 1103 20-21 0.8196749120185913 1103 22-23 0.8395977519510964 1103 24-25 0.828009620201783 1103 26-27 0.796853987665699 1103 28-29 0.8508474113807196 1103 30-31 0.776885508529908 1103 32-33 0.7787213517699172 1103 34-35 0.8019250243593277 1103 36-37 0.7278823022834189 1103 38-39 0.8377253873620205 1103 40-41 0.7913450435119955 1103 42-43 0.794890062255881 1103 44-45 0.7444267911779079 1103 46-47 0.7630890853304777 1103 48-49 0.6801270472349898 1103 50-51 0.5957071456076832 1103 52-53 0.6379628026067365 1103 54-55 0.6217883517074867 1103 56-57 0.6978248111045176 1103 58-59 0.7308523277889734 1103 60-61 0.7666134160695641 1103 62-63 0.6179132774740097 1103 64-65 0.5257095879989642 1103 66-67 0.5256873836741782 1103 68-69 0.6628688891049599 1103 70-71 0.5655994921549024 1103 72-73 0.49109123569590807 1103 74-75 0.47792000892355446 1103 76-77 0.5618063877036441 1103 78-79 0.5113833851612561 1103 80-81 0.5256674302971263 1103 82-83 0.59624157746903 1103 84-85 0.6473126504293738 1103 86-87 0.7358840579982058 1103 88-89 0.7321377528267234 1103 90-91 0.6740302100227709 1103 92-93 0.680324777284735 1103 94-95 0.7154539748344568 1103 96-97 0.6823652664761823 1103 98-99 0.695276748271251 1103 100-101 0.7381339038674355 1103 102-103 0.4864190675402362 1103 104-105 0.5164582507464921 1103 106-107 0.6623668954360582 1103 108-109 0.7236269937730277 1103 110-111 0.6631666803579606 1103 112-113 0.6269653469497527 1103 114-115 0.689292917558948 1103 116-117 0.722043489845781 1103 118-119 0.6018946676344861 1103 120-121 0.5495571606088561 1103 122-123 0.5354575720943373 1103 124-125 0.72143787506511 1104 1 0.633060934919218 1104 2 0.7961566922020857 1104 3 0.4996666677861157 1104 4 0.3095952929301262 1104 5 0.5940101077714957 1104 6 0.7526075902301059 1104 7 0.9076054366395709 1104 8 0.7085403548024445 1104 9 0.8552764017961891 1104 10-11 0.9072434311217279 1104 12-13 0.8575427583028628 1104 14-15 1.0151411709038172 1104 16-17 0.9823505980638458 1104 18-19 0.9198160508349957 1104 20-21 0.848275345889526 1104 22-23 0.9018025832012313 1104 24-25 0.9331931089350434 1104 26-27 0.969625043013636 1104 28-29 1.1558664268815306 1104 30-31 1.0192201165216304 1104 32-33 1.1446866541803828 1104 34-35 1.2762520382362794 1104 36-37 1.374925162557524 1104 38-39 1.3462948602730975 1104 40-41 1.1677547257885976 1104 42-43 1.2061739477794333 1104 44-45 1.1726944331843896 1104 46-47 1.174527061507682 1104 48-49 1.2127981112076043 1104 50-51 1.1250884702599322 1104 52-53 1.1792799728101642 1104 54-55 0.8238086147657739 1104 56-57 1.029170805324334 1104 58-59 1.133217358384627 1104 60-61 1.1387558035243295 1104 62-63 1.0789558092411298 1104 64-65 1.1572472479195177 1104 66-67 1.1138327309879195 1104 68-69 0.8069043722546958 1104 70-71 0.5309170095429785 1104 72-73 0.5649407608165298 1104 74-75 0.9034785669426704 1104 76-77 1.0641317557495853 1104 78-79 1.046451506414396 1104 80-81 1.1061379094394042 1104 82-83 1.026205590022613 1104 84-85 1.0605755433674098 1104 86-87 1.0640900088089538 1104 88-89 1.053624142584333 1104 90-91 1.0982560624242481 1104 92-93 1.042269439787752 1104 94-95 1.0881451557401824 1104 96-97 1.0875508976463308 1104 98-99 1.1050559838827283 1104 100-101 1.1271268321776482 1104 102-103 0.9212436651329092 1104 104-105 1.026938337894233 1104 106-107 0.9977895625282827 1104 108-109 1.0140680995561233 1104 110-111 1.054043305107939 1104 112-113 1.0342052454971231 1104 114-115 1.0617882577586997 1104 116-117 1.0603565240473145 1104 118-119 1.0410511078272613 1104 120-121 1.0904930869134226 1104 122-123 1.1944176002646323 1104 124-125 1.5590716469021721 1105 1 0.23486237306832436 1105 2 0.051787233964301294 1105 3 0.19703963126888624 1105 4 0.22076802562681763 1105 5 -0.18593704778734121 1105 6 0.36316240254512167 1105 7 0.47294008227678574 1105 8 0.6210195716785876 1105 9 0.7342537700635603 1105 10-11 0.6664695008391135 1105 12-13 0.6192740155081324 1105 14-15 0.6906072261759277 1105 16-17 0.76883323956271 1105 18-19 0.7602633484333481 1105 20-21 0.8038349416111643 1105 22-23 0.9204044829535434 1105 24-25 0.8826903216472246 1105 26-27 0.9542201768586267 1105 28-29 0.9138672942498332 1105 30-31 0.9752199532596109 1105 32-33 1.1030459142837117 1105 34-35 1.0843828828169606 1105 36-37 1.0996591032659424 1105 38-39 1.105070368703558 1105 40-41 0.9499025044589899 1105 42-43 0.8897025010582951 1105 44-45 1.0200258944361522 1105 46-47 1.0307270494182177 1105 48-49 0.9438641974870876 1105 50-51 0.6608638046425241 1105 52-53 0.8096901709756565 1105 54-55 0.6381689065857827 1105 56-57 0.6768941109551108 1105 58-59 0.3580648220694904 1105 60-61 0.4460354197716647 1105 62-63 0.687240045633061 1105 64-65 0.5059358247534291 1105 66-67 0.6404078545266003 1105 68-69 0.6442647838053617 1105 70-71 0.8396435975629721 1105 72-73 0.7241903343682097 1105 74-75 0.6113635407761286 1105 76-77 0.7139237712588553 1105 78-79 0.5878107279525047 1105 80-81 0.6267279744020158 1105 82-83 0.6394780865247789 1105 84-85 0.5986790299195128 1105 86-87 0.6317545525261252 1105 88-89 0.5521847752215763 1105 90-91 0.443241834806102 1105 92-93 0.6087131265329333 1105 94-95 0.5484829203494002 1105 96-97 0.5676980724644984 1105 98-99 0.40389059254543724 1105 100-101 0.520786725254986 1105 102-103 0.24471388482819023 1105 104-105 0.42966509305605527 1105 106-107 0.5229305564355187 1105 108-109 0.39459265485527695 1105 110-111 0.30605620404944744 1105 112-113 0.18925594324441164 1105 114-115 0.3084334520548673 1105 116-117 0.10807124755834252 1105 118-119 0.16081897312740168 1105 120-121 0.22373972997382552 1105 122-123 0.286106763957239 1105 124-125 0.6712566739897774 1106 1 -0.11386286601760887 1106 2 -0.5401490206827049 1106 3 -0.08160742608486515 1106 4 -0.22933590291447814 1106 5 0.12502128069411356 1106 6 0.21652494304362335 1106 7 0.36415007073126304 1106 8 0.25034723643954493 1106 9 0.2957503630585876 1106 10-11 0.22149666589133687 1106 12-13 0.2246655865396434 1106 14-15 0.17913234290794122 1106 16-17 0.12776098962254423 1106 18-19 0.2651958879905436 1106 20-21 0.23733352187515777 1106 22-23 0.33836241872700157 1106 24-25 0.4085500009089884 1106 26-27 0.49411680932873736 1106 28-29 0.572734173786543 1106 30-31 0.480181918414992 1106 32-33 0.3785272251395071 1106 34-35 0.47205036711454085 1106 36-37 0.4819432352705135 1106 38-39 0.38999399650816 1106 40-41 0.3182828252475147 1106 42-43 0.18359484689841565 1106 44-45 0.2090857556101895 1106 46-47 0.006318771089937059 1106 48-49 0.2325851024849186 1106 50-51 0.2753008202692797 1106 52-53 0.11860509006036324 1106 54-55 0.06302751835441711 1106 56-57 -0.062215156937575955 1106 58-59 0.32997932613147896 1106 60-61 0.1816760421517465 1106 62-63 0.0949960204438014 1106 64-65 0.15445216173553789 1106 66-67 -0.04059662146438825 1106 68-69 0.07749040137755969 1106 70-71 0.20210736950508235 1106 72-73 0.24869820400675735 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-1.3126023727248644 2216 104-105 -1.3215175659156166 2216 106-107 -1.4186323118126651 2216 108-109 -1.3906955268221388 2216 110-111 -1.3412232757074563 2216 112-113 -1.3166070489669899 2216 114-115 -1.496212172358451 2216 116-117 -1.5212201149960194 2216 118-119 -1.4464630130135667 2216 120-121 -1.6720327480920325 2216 122-123 -1.5839093372639326 2216 124-125 -1.8336122941760813 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 74472.0 3 25532.0 4 12388.0 5 9893.0 6 10847.0 7 12872.0 8 14596.0 9 15852.0 10 17099.0 11 19259.0 12 20897.0 13 23872.0 14 27445.0 15 23894.0 16 16271.0 17 13751.0 18 12867.0 19 12248.0 20 12103.0 21 12518.0 22 13847.0 23 17216.0 24 22173.0 25 29254.0 26 36583.0 27 40572.0 28 46986.0 29 55708.0 30 64447.0 31 75309.0 32 91578.0 33 114374.0 34 146618.0 35 202917.0 36 327492.0 37 480531.0 38 164095.0 39 461.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.67171140526762 19.669430049576857 14.09014484558222 23.568713699573298 2 15.617059593692698 20.880519364025744 39.1746117448582 24.32780929742336 3 18.88080102223658 25.918596261832977 29.251862032562016 25.94874068336843 4 12.763826514599794 17.048596616268867 36.81723267241889 33.37034419671245 5 13.148056547312295 38.731959167461966 33.66653197270873 14.453452312517006 6 31.2913758060614 37.27472004284907 17.07981199195976 14.354092159129769 7 27.89873463577167 33.12079331406745 20.938778555438013 18.041693494722868 8 26.272988665616936 35.13719127596232 19.482105682631236 19.107714375789506 9 26.939780277409042 15.62873316651874 19.687011319068702 37.74447523700351 10-11 25.150588283493025 27.000967085665245 26.740058801395968 21.108385829445766 12-13 26.035831755315268 24.24704711887899 27.725299363430896 21.991821762374848 14-15 23.067888371579535 25.237597656755373 26.00481448450861 25.689699487156485 16-17 22.13351606920647 28.596927889696072 26.531079581314625 22.738476459782834 18-19 22.059172659194523 26.50832405946117 29.656452361694598 21.776050919649705 20-21 23.155750253794846 25.665020126772358 29.68418605814554 21.49504356128725 22-23 22.784093922944994 25.435379890867704 29.487281770991235 22.293244415196064 24-25 22.35739295172537 25.854663350636546 29.602986324610136 22.184957373027945 26-27 22.132193854074263 26.084174954945087 29.523485264380376 22.260145926600273 28-29 22.169108911061883 25.96374820653629 29.70928961371584 22.157853268685983 30-31 22.150996383100665 25.896451540147066 29.600032257549795 22.35251981920247 32-33 22.199533645530064 25.965279146399684 29.253673285358133 22.58151392271212 34-35 22.465270305761035 25.90063467160477 29.439887322826053 22.194207699808135 36-37 22.72755696066606 25.666293059839912 29.21169103304803 22.394458946446 38-39 22.44077526794682 25.861261485822418 29.3212502646801 22.376712981550664 40-41 22.516610697517763 25.826394869497083 29.13300934908318 22.523985083901973 42-43 22.43051150210213 25.89809029267689 28.843942027835507 22.827456177385475 44-45 22.560727640623295 26.004091706316572 28.688993663633966 22.746186989426164 46-47 22.817494287006806 26.381802601907765 28.086256170657965 22.714446940427464 48-49 22.747480741423395 26.4625327265349 27.788003210229956 23.001983321811753 50-51 22.8766187532802 26.759233184566227 27.443067365235247 22.921080696918324 52-53 23.141708537512553 26.55941318859411 27.431294222060455 22.86758405183288 54-55 22.91685444039404 26.486963076749248 27.501739018309614 23.094443464547098 56-57 22.693894397445426 26.750171596549826 27.27139813828256 23.284535867722187 58-59 22.790540617758303 26.318413313326026 27.496814650730684 23.394231418184987 60-61 22.973585465472564 26.430232051670732 27.611427625141395 22.984754857715313 62-63 22.769194212443566 26.481486193294312 27.68978587110694 23.059533723155184 64-65 22.66662009146421 26.296510245142972 27.655923966844494 23.380945696548324 66-67 22.594774880683723 26.62166422219414 27.506698400965657 23.27686249615648 68-69 22.837849318429885 26.315346874316738 27.70393089294332 23.14287291431006 70-71 22.63572213139604 26.289773709838165 27.67283771994323 23.401666438822566 72-73 22.852878404131037 26.49668777926176 27.213210760394112 23.437223056213092 74-75 22.8265064927616 26.621465379053856 27.238483655214974 23.313544472969575 76-77 22.985962359579393 26.540610659567704 27.280313363983755 23.193113616869145 78-79 22.8749368756838 26.56417850845057 27.23244454008626 23.328440075779366 80-81 22.59623905970024 26.714664907162433 27.381314323787592 23.307781709349737 82-83 22.98915361450589 26.732107517690977 26.97252976384282 23.306209103960303 84-85 22.804837079967243 26.60130919077106 27.060159899121842 23.53369383013985 86-87 22.81139209008654 26.51488655735612 27.190591662975876 23.483129689581457 88-89 22.76668370130304 26.547618277517948 27.19241160856562 23.493286412613394 90-91 22.61558272530583 26.821203905233524 27.153870668787842 23.409342700672795 92-93 22.72468418232413 26.673868722055648 27.034359626226262 23.56708746939396 94-95 22.63171150020463 26.43454455832816 27.15839880077815 23.775345140689062 96-97 22.72460757867888 26.44813284629695 27.11828588150128 23.70897369352289 98-99 22.499333690423516 26.426872847431927 27.098508587846343 23.975284874298218 100-101 22.422775223037256 26.343689771746643 27.27137468232258 23.962160322893517 102-103 22.645597771641558 26.182952919933573 27.153956918920997 24.017492389503875 104-105 22.520405703376305 26.200224509096586 27.235872982878917 24.043496804648193 106-107 22.48724612737032 26.276546264876465 27.27421510773543 23.96199250001779 108-109 22.57344953526272 26.150760057735837 26.9231946876818 24.35259571931964 110-111 22.4395488336921 26.133818519605427 27.182664394548212 24.24396825215426 112-113 22.568792028072693 26.18661855059239 27.0820674329416 24.16252198839332 114-115 22.349371689342547 26.437132062322622 26.935851894721363 24.27764435361347 116-117 22.177151735977993 26.339302848798773 27.04364299862388 24.439902416599356 118-119 22.179178614106988 26.35208943103806 26.974039141172923 24.494692813682033 120-121 22.211685233258102 26.25489177556386 27.082879168265006 24.450543822913033 122-123 22.271454238232547 26.283312474177627 26.974058168149234 24.471175119440588 124-125 22.072244716330378 26.25530719240296 26.812904985664055 24.859543105602608 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 397.0 1 315.0 2 442.5 3 695.0 4 523.0 5 371.0 6 758.5 7 1780.0 8 3022.0 9 3459.5 10 2642.5 11 1771.0 12 1553.0 13 1623.0 14 1870.5 15 2059.0 16 2294.0 17 2629.0 18 2972.0 19 3238.5 20 3602.5 21 4272.0 22 5124.5 23 6183.0 24 7501.5 25 9279.0 26 11789.0 27 14778.0 28 17953.0 29 21651.0 30 25914.5 31 31107.0 32 35976.0 33 39113.5 34 42451.5 35 46239.5 36 50691.5 37 55518.5 38 58490.0 39 60966.5 40 62928.5 41 65346.5 42 69670.0 43 74134.0 44 77646.0 45 79747.5 46 80967.5 47 81501.5 48 84978.5 49 87224.5 50 85572.5 51 84079.5 52 83324.0 53 84237.5 54 82501.5 55 76746.0 56 70791.5 57 66317.0 58 63453.0 59 58688.5 60 50328.5 61 40847.0 62 32048.0 63 23584.5 64 16485.0 65 12222.5 66 9438.0 67 7263.5 68 5537.0 69 4282.0 70 3225.5 71 2490.5 72 2072.5 73 1808.5 74 1435.5 75 1114.0 76 756.5 77 421.0 78 274.5 79 164.0 80 110.0 81 82.0 82 62.0 83 35.0 84 18.0 85 16.0 86 12.5 87 9.5 88 8.0 89 4.5 90 2.0 91 2.0 92 1.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09625514859388563 2 4.312506657432152E-5 3 0.0 4 0.10350015977837167 5 0.0 6 0.0 7 0.0025012538613106484 8 0.006037509320405013 9 0.004096881324560544 10-11 6.253134653276621E-4 12-13 0.0 14-15 4.312506657432152E-5 16-17 3.2343799930741144E-4 18-19 0.002005315595705951 20-21 0.0015093773301012533 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.007395948917496142 58-59 7.546886650506267E-4 60-61 0.0 62-63 0.0 64-65 2.803129327330899E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0010350015977837166 76-77 0.0 78-79 0.0 80-81 8.625013314864304E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0013800021303782886 90-91 0.0 92-93 0.002350316128300523 94-95 0.0 96-97 0.008085949982685286 98-99 0.004096881324560544 100-101 0.0023287535950133626 102-103 0.0 104-105 0.0 106-107 0.001358439597091128 108-109 0.0 110-111 1.0781266643580382E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 9.271889313479129E-4 122-123 0.004786882389749689 124-125 0.08340387875473783 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2318837.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.55518978548599 #Duplication Level Percentage of deduplicated Percentage of total 1 72.82680854251404 33.90465893167994 2 12.604829480208668 11.736404573296067 3 5.319219548174248 7.429118267277575 4 2.72716458610831 5.07854659530115 5 1.6353409871579898 3.806680501056211 6 1.0520699581161113 2.9387589940622316 7 0.7333408797721134 2.3898576684672204 8 0.5090543534622703 1.8959297629251093 9 0.3904417808935242 1.6359382088713041 >10 1.9839527152356442 16.569569358419926 >50 0.13961050357251076 4.480304159745698 >100 0.07481127297304174 6.138161387176339 >500 0.0026097491867939637 0.7705102770416493 >1k 5.592319685986606E-4 0.5556335021409958 >5k 1.8641065619955354E-4 0.6699278125384486 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8492 0.3662180653491384 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6947 0.2995898374918116 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4502 0.1941490497175955 No Hit ATCCAATACAGTGGCCTCGTGTGGCTGTATAACTCGGTTCACTTCAGACT 2347 0.10121453124993263 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 8.625013314864305E-5 0.0 0.0 0.0 0.0 3 8.625013314864305E-5 0.0 0.0 0.0 0.0 4 8.625013314864305E-5 0.0 0.0 0.0 0.0 5 8.625013314864305E-5 0.0 0.0 0.0 0.0 6 1.2937519972296458E-4 0.0 0.0 0.0 0.0 7 2.156253328716076E-4 0.0 0.0 0.0 0.0 8 2.156253328716076E-4 0.0 0.0 0.0 0.0 9 2.156253328716076E-4 0.0 0.0 0.0 0.0 10-11 2.5875039944592916E-4 2.1562533287160763E-5 0.0 0.0 0.0 12-13 3.2343799930741144E-4 4.312506657432153E-5 0.0 0.0 0.0 14-15 3.881255991688937E-4 4.312506657432153E-5 0.0 0.0 0.0 16-17 4.312506657432152E-4 4.312506657432153E-5 0.0 4.312506657432153E-5 0.0 18-19 5.606258654661798E-4 4.312506657432153E-5 0.0 6.468759986148229E-5 0.0 20-21 6.900010651891444E-4 4.312506657432153E-5 0.0 1.5093773301012533E-4 0.0 22-23 7.762511983377874E-4 4.312506657432153E-5 0.0 2.803129327330899E-4 0.0 24-25 9.91876531209395E-4 4.312506657432153E-5 0.0 6.037509320405013E-4 0.0 26-27 9.91876531209395E-4 4.312506657432153E-5 0.0 9.271889313479129E-4 0.0 28-29 0.001185939330793842 4.312506657432153E-5 0.0 0.0017465651962600215 0.0 30-31 0.0012937519972296458 4.312506657432153E-5 0.0 0.004096881324560544 0.0 32-33 0.0013153145305168064 4.312506657432153E-5 0.0 0.009358139446627771 0.0 34-35 0.0014015646636654495 6.468759986148229E-5 0.0 0.015934712099211802 0.0 36-37 0.0015525023966755749 8.625013314864305E-5 0.0 0.027319729674832684 0.0 38-39 0.0016818775963985395 8.625013314864305E-5 0.0 0.047394448165179355 0.0 40-41 0.0017465651962600217 8.625013314864305E-5 0.0 0.07031542104943125 0.0 42-43 0.0020268781289931115 8.625013314864305E-5 0.0 0.09353826939970339 0.0 44-45 0.0021993783952903977 8.625013314864305E-5 0.0 0.12154800013972522 0.0 46-47 0.002609066527746452 8.625013314864305E-5 0.0 0.15473273886866562 0.0 48-49 0.0026737541276079346 1.2937519972296458E-4 0.0 0.19113029505739298 0.0 50-51 0.0026737541276079346 1.2937519972296458E-4 0.0 0.23444942443129896 0.0 52-53 0.0026737541276079346 1.2937519972296458E-4 0.0 0.2768629274071442 0.0 54-55 0.0026737541276079346 1.2937519972296458E-4 0.0 0.32182080931087437 0.0 56-57 0.0028462543939052204 1.2937519972296458E-4 0.0 0.37637401852739105 0.0 58-59 0.002889379460479542 1.2937519972296458E-4 0.0 0.4418809946537855 0.0 60-61 0.0029325045270538635 1.2937519972296458E-4 0.0 0.5151936078301321 0.0 62-63 0.002997192126915346 1.2937519972296458E-4 0.0 0.5918484136659886 0.0 64-65 0.0030187546602025066 1.2937519972296458E-4 0.0 0.6683307192355479 0.0 66-67 0.003148129859925471 1.2937519972296458E-4 0.0 0.7556805415818361 0.0 68-69 0.003148129859925471 1.2937519972296458E-4 0.0 0.859892264958684 0.0 70-71 0.003342192659509918 1.2937519972296458E-4 0.0 0.979930887768308 0.0 72-73 0.003428442792658561 1.2937519972296458E-4 0.0 1.1026001396389655 0.0 74-75 0.003514692925807204 1.2937519972296458E-4 0.0 1.2241481397786909 0.0 76-77 0.0036656306588173297 1.2937519972296458E-4 0.0 1.3598627242880807 0.0 78-79 0.0036871931921044904 1.2937519972296458E-4 0.0 1.5209995355430328 0.0 80-81 0.004140006391134867 1.2937519972296458E-4 0.0 1.7084857624748957 0.0 82-83 0.004226256524283509 1.2937519972296458E-4 0.0 1.9082841959137276 0.0 84-85 0.004377194257293635 1.2937519972296458E-4 0.0 2.104632624026613 0.0 86-87 0.00452813199030376 1.2937519972296458E-4 0.0 2.30917481478862 0.0 88-89 0.00452813199030376 1.2937519972296458E-4 0.0 2.541662048690788 0.0 90-91 0.004873132522898333 1.2937519972296458E-4 0.0 2.8058893315916555 0.0 92-93 0.005024070255908458 1.2937519972296458E-4 0.0 3.098061657632684 0.0 94-95 0.005045632789195618 1.2937519972296458E-4 0.0 3.391829611136962 0.0 96-97 0.005045632789195618 1.2937519972296458E-4 0.0 3.6873656923707876 0.0 98-99 0.0050887578557699395 1.2937519972296458E-4 0.0 4.010437128612317 0.0 100-101 0.005196570522205743 1.2937519972296458E-4 0.0 4.386940522339431 0.0 102-103 0.005412195855077351 1.2937519972296458E-4 0.0 4.800811786253195 0.0 104-105 0.006555010119296872 1.2937519972296458E-4 0.0 5.213303048036581 0.0 106-107 0.006856885585317123 1.2937519972296458E-4 0.0 5.629589315678507 0.0 108-109 0.006986260785040087 1.2937519972296458E-4 0.0 6.0639449862150725 0.0 110-111 0.007094073451475891 1.2937519972296458E-4 0.0 6.554169180498672 0.0 112-113 0.007158761051337373 1.2937519972296458E-4 0.0 7.091830948014026 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5980 0.0 59.52111 1 GTATCAA 9590 0.0 54.33638 1 ATCAACG 13000 0.0 40.21744 3 TATCAAC 13040 0.0 39.591763 2 CAACGCA 13150 0.0 39.498177 5 TCAACGC 13280 0.0 39.279808 4 AACGCAG 13465 0.0 38.839275 6 GTACATG 12465 0.0 33.29008 1 ACGCAGA 15920 0.0 32.70044 7 CGCAGAG 16215 0.0 31.922054 8 TACATGG 12975 0.0 31.299711 2 GTGGTAT 2415 0.0 31.10651 1 ACATGGG 13460 0.0 29.02165 3 GCAGAGT 17320 0.0 28.95798 9 TATCACG 145 4.0440573E-6 28.747053 2 TGGTATC 2675 0.0 26.935492 2 ACCGTAT 205 7.781455E-8 26.120243 8 GAGTACT 10245 0.0 26.016844 12-13 CATGGGG 5510 0.0 25.612976 4 AGTACTT 10585 0.0 25.490301 12-13 >>END_MODULE