FastQCFastQC Report
Thu 26 May 2016
SRR1364529_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364529_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816766
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41540.22864804823516072No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29760.16380755694459276No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25070.13799245472449398No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA18820.10359066605165443No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG137100.036.717251
GTATCAA106200.036.1386951
TACATGG138350.035.7833862
GGTATCA70300.033.433281
ACATGGG143750.033.362953
ATCAACG121950.030.641863
TCAACGC123000.030.4770344
CAACGCA123800.030.3281565
AACGCAG125650.029.7819866
CATGGGA100350.029.5290344
TATCAAC129400.029.4295062
CATGGGG45900.027.4827674
ATGGGAG39950.027.4055375
ACGCAGA141750.026.063587
CGCAGAG141300.025.4329178
ATGGGAT36500.025.4313265
AGTACAT98500.023.6198862
GAGTACT46850.023.36993212-13
GCAGAGT149050.023.1531129
TAGACGC1300.002052058222.8856854
Produced by FastQC (version 0.11.5)