##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364529_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1816766 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.15316667088662 31.0 30.0 34.0 24.0 34.0 2 29.149704474874586 31.0 30.0 34.0 23.0 34.0 3 29.399142762469136 31.0 30.0 34.0 24.0 34.0 4 32.7241350839899 35.0 33.0 37.0 25.0 37.0 5 32.95574884162297 35.0 33.0 37.0 26.0 37.0 6 32.89715846729848 37.0 33.0 37.0 26.0 37.0 7 32.70887665224911 36.0 33.0 37.0 25.0 37.0 8 32.67346427663222 36.0 33.0 37.0 25.0 37.0 9 33.79201779425639 38.0 34.0 39.0 22.0 39.0 10-11 33.74446626588124 38.0 33.5 39.0 21.0 39.0 12-13 33.67970393545454 38.0 33.5 39.0 20.0 39.0 14-15 34.47974422682943 39.0 33.0 41.0 18.0 41.0 16-17 34.65057635380671 39.0 34.0 41.0 19.0 41.0 18-19 34.475605829259244 39.0 33.5 41.0 17.5 41.0 20-21 34.2070255608042 39.0 33.0 41.0 18.0 41.0 22-23 34.147653853055374 39.0 33.0 41.0 18.0 41.0 24-25 33.98827862256339 39.0 32.5 41.0 18.0 41.0 26-27 33.92817594560884 38.5 32.5 41.0 17.5 41.0 28-29 34.01177256729815 38.5 33.0 41.0 17.5 41.0 30-31 33.964819630045916 38.0 33.0 41.0 18.0 41.0 32-33 33.940513527884164 38.0 33.0 40.0 18.0 41.0 34-35 33.4508951620627 38.0 32.0 40.0 15.5 41.0 36-37 33.30513423302726 38.0 32.0 40.0 14.5 41.0 38-39 33.09540771899078 38.0 31.5 40.0 11.0 41.0 40-41 33.309311986243685 38.0 32.0 40.0 15.0 41.0 42-43 33.20119211830252 38.0 32.0 40.0 11.5 41.0 44-45 33.03322194492851 38.0 32.0 40.0 9.0 41.0 46-47 32.944315338353974 38.0 32.0 40.0 8.5 41.0 48-49 32.76054208412091 38.0 32.0 40.0 7.0 41.0 50-51 32.60345195803973 38.0 31.0 40.0 2.0 41.0 52-53 32.53338900001431 38.0 31.5 40.0 2.0 41.0 54-55 32.553632663755266 38.0 31.0 40.0 2.0 41.0 56-57 32.432926695017414 37.5 31.0 40.0 2.0 41.0 58-59 32.231836406009364 37.0 31.0 40.0 2.0 41.0 60-61 32.02375512311437 37.0 31.0 40.0 2.0 41.0 62-63 31.754008771630467 36.0 31.0 40.0 2.0 41.0 64-65 31.470904618426367 36.0 30.0 39.5 2.0 41.0 66-67 31.210152270573097 35.5 30.0 39.0 2.0 41.0 68-69 31.160106199697704 35.0 30.0 39.0 2.0 41.0 70-71 30.90927477726906 35.0 30.0 39.0 2.0 40.5 72-73 30.617193683721514 35.0 30.0 38.0 2.0 40.0 74-75 30.29629214769541 35.0 30.0 37.0 2.0 39.5 76-77 29.972296927617535 35.0 30.0 37.0 2.0 39.0 78-79 29.651474102883917 35.0 29.5 36.0 2.0 39.0 80-81 29.33974078114628 35.0 29.0 36.0 2.0 38.0 82-83 29.074981863377012 34.0 29.0 35.5 2.0 37.0 84-85 28.819218050095607 34.0 29.0 35.0 2.0 37.0 86-87 28.596130431767214 34.0 29.0 35.0 2.0 36.0 88-89 28.389101293177 34.0 29.0 35.0 2.0 36.0 90-91 28.236491105623948 34.0 29.0 35.0 2.0 36.0 92-93 28.106007047688035 34.0 29.0 35.0 2.0 35.5 94-95 27.986119015877662 34.0 28.5 35.0 2.0 35.0 96-97 27.870443414286704 34.0 28.0 35.0 2.0 35.0 98-99 27.76987927999533 34.0 28.0 35.0 2.0 35.0 100-101 27.66766055727595 34.0 28.0 35.0 2.0 35.0 102-103 26.487659390367277 32.5 25.0 34.0 2.0 35.0 104-105 27.34524148954791 34.0 27.0 35.0 2.0 35.0 106-107 27.485362176526863 34.0 27.0 35.0 2.0 35.0 108-109 27.430765987474445 34.0 27.0 35.0 2.0 35.0 110-111 27.33122399912812 34.0 27.0 35.0 2.0 35.0 112-113 27.223326504348936 34.0 27.0 35.0 2.0 35.0 114-115 27.08437300125608 34.0 27.0 35.0 2.0 35.0 116-117 26.968781064815172 34.0 26.5 35.0 2.0 35.0 118-119 26.827225960855717 34.0 25.5 35.0 2.0 35.0 120-121 26.644058728531906 34.0 25.0 35.0 2.0 35.0 122-123 26.400310496783845 34.0 25.0 35.0 2.0 35.0 124-125 24.946914462291787 32.0 21.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.9384366582191817 1101 2 0.9789803720560748 1101 3 0.9060667296784004 1101 4 1.0131631901478002 1101 5 0.8668651991681173 1101 6 0.9005959251060389 1101 7 0.9890122710513296 1101 8 0.88252876703163 1101 9 1.1066388311007032 1101 10-11 1.3062147941620879 1101 12-13 0.9871360513781369 1101 14-15 1.33020484294547 1101 16-17 1.1065521432447127 1101 18-19 1.4816036146700498 1101 20-21 1.798568354001354 1101 22-23 1.6687854462717837 1101 24-25 1.8614215373044942 1101 26-27 2.0100695649102818 1101 28-29 1.8508679513522637 1101 30-31 1.8679881125936575 1101 32-33 1.7561939824174502 1101 34-35 2.045439837209152 1101 36-37 1.9795377775074954 1101 38-39 1.7773189948241424 1101 40-41 1.5298865510436173 1101 42-43 1.6000283090200327 1101 44-45 1.460074183857742 1101 46-47 1.250423445490057 1101 48-49 1.2404163282547955 1101 50-51 1.2504204071457394 1101 52-53 1.169075167562596 1101 54-55 1.0517907516514384 1101 56-57 1.0995257522343351 1101 58-59 1.119895461592364 1101 60-61 1.0344120863714679 1101 62-63 1.079288243457185 1101 64-65 1.0566558803167396 1101 66-67 1.065148437765938 1101 68-69 0.8949131280997484 1101 70-71 0.8926899392372682 1101 72-73 0.7873178010929891 1101 74-75 0.8241136659552843 1101 76-77 0.8783884338660677 1101 78-79 0.9447482899651298 1101 80-81 0.9728258480024081 1101 82-83 0.7899768244751897 1101 84-85 0.8542972667998789 1101 86-87 0.8297739840234755 1101 88-89 0.8867043111373363 1101 90-91 0.9275561319893342 1101 92-93 0.7271256517003302 1101 94-95 0.7935621472305634 1101 96-97 0.7641397719042899 1101 98-99 0.8810353347733795 1101 100-101 0.954949669061083 1101 102-103 0.8010683177224749 1101 104-105 0.8592263637067958 1101 106-107 0.7853503948364882 1101 108-109 0.8821079651657264 1101 110-111 0.7223345841505306 1101 112-113 0.7316832496877588 1101 114-115 0.8677192266826559 1101 116-117 0.9716457175209534 1101 118-119 0.8323545645802604 1101 120-121 0.9108088728796879 1101 122-123 1.0211296044057825 1101 124-125 1.380096515254536 1102 1 0.10880534215798576 1102 2 0.3584464462014978 1102 3 0.442311443870814 1102 4 0.605959678802698 1102 5 0.5740165365367247 1102 6 0.12475123572362179 1102 7 -0.38788961633314756 1102 8 0.3566292402459439 1102 9 0.6758322506123093 1102 10-11 0.831628336775097 1102 12-13 0.7653908865922858 1102 14-15 1.0791114880128347 1102 16-17 0.9009606983174479 1102 18-19 0.9272870755194944 1102 20-21 1.3451141083896232 1102 22-23 1.1929646912450238 1102 24-25 1.2721174213979936 1102 26-27 0.89949919024275 1102 28-29 1.2970852650856912 1102 30-31 1.1657215728958334 1102 32-33 1.1320647952734006 1102 34-35 1.4619770905229217 1102 36-37 1.6504492897136487 1102 38-39 1.7033882512172767 1102 40-41 1.402428226375541 1102 42-43 1.220177898742854 1102 44-45 1.302984907981461 1102 46-47 1.0974925449964132 1102 48-49 0.7880991104777024 1102 50-51 1.0208250655886175 1102 52-53 0.9128938648185354 1102 54-55 0.7872415457539574 1102 56-57 0.7402521745224675 1102 58-59 0.9539573333611315 1102 60-61 0.8416156706871618 1102 62-63 0.7883813283986072 1102 64-65 0.8988393794757599 1102 66-67 0.8173634967079373 1102 68-69 0.7535593775935148 1102 70-71 0.7659282648539616 1102 72-73 0.7909414479444941 1102 74-75 0.5678261180227224 1102 76-77 0.7584885495244613 1102 78-79 0.6975352924424847 1102 80-81 0.46506228732917876 1102 82-83 0.5006003947075612 1102 84-85 0.2264311824385743 1102 86-87 0.5047546285727549 1102 88-89 0.7950798032327953 1102 90-91 0.35372601483503985 1102 92-93 0.7112643250219151 1102 94-95 0.6235980852493377 1102 96-97 0.8035619177506206 1102 98-99 0.879574828284472 1102 100-101 0.8916585492178264 1102 102-103 0.7582761708180676 1102 104-105 0.9417526787281574 1102 106-107 0.8205965793864252 1102 108-109 0.47668595796352875 1102 110-111 0.708703530783481 1102 112-113 0.40903237385817093 1102 114-115 0.4961802403172122 1102 116-117 0.48631331821125556 1102 118-119 0.7884631886102049 1102 120-121 1.0929982826570175 1102 122-123 0.8966654822238205 1102 124-125 1.075781982795391 1103 1 0.7112497882209574 1103 2 0.812108890938898 1103 3 0.6811101241234034 1103 4 0.8758864808915732 1103 5 0.8734835392097224 1103 6 0.9755405349901878 1103 7 0.9343210334166727 1103 8 1.0059082590297663 1103 9 1.194461519580038 1103 10-11 1.341235034566047 1103 12-13 1.2970789542402628 1103 14-15 1.6293522763652675 1103 16-17 1.4031902120065496 1103 18-19 1.4987895627917993 1103 20-21 1.930783341231809 1103 22-23 1.8279721567036162 1103 24-25 1.92094931383712 1103 26-27 1.8929578461688692 1103 28-29 1.8316267402603117 1103 30-31 1.7389282969069342 1103 32-33 1.6890943439227044 1103 34-35 2.0377180646013926 1103 36-37 2.001101370209497 1103 38-39 2.0292025688743607 1103 40-41 1.702377461257079 1103 42-43 1.5797953759276808 1103 44-45 1.4325615982705386 1103 46-47 1.417793712521302 1103 48-49 1.4331102176234154 1103 50-51 1.4925239854642243 1103 52-53 1.586056597249211 1103 54-55 1.3774247954498762 1103 56-57 1.3939793684568826 1103 58-59 1.26993712928099 1103 60-61 1.2148223946982668 1103 62-63 1.18924491808243 1103 64-65 1.3937534720765825 1103 66-67 1.3402545592794048 1103 68-69 1.3671798700952742 1103 70-71 1.3899707560592844 1103 72-73 1.2827200479342942 1103 74-75 1.2889124332908608 1103 76-77 1.2728026377552588 1103 78-79 1.2599311583992652 1103 80-81 1.3136833989177568 1103 82-83 1.2890291523824189 1103 84-85 1.3486371708288765 1103 86-87 1.1683598988223558 1103 88-89 1.2754613792093146 1103 90-91 1.4102977305795115 1103 92-93 1.424098795775528 1103 94-95 1.337778669230282 1103 96-97 1.4027846360515568 1103 98-99 1.344308001869127 1103 100-101 1.2560425578413401 1103 102-103 1.2336951469213169 1103 104-105 1.2211306785679241 1103 106-107 1.317223357440163 1103 108-109 1.416921065284047 1103 110-111 1.4590558275955487 1103 112-113 1.5522407002116054 1103 114-115 1.4960386515219568 1103 116-117 1.4943984462893916 1103 118-119 1.5719003585823117 1103 120-121 1.6412028580727167 1103 122-123 1.6874268861670352 1103 124-125 1.8142328819279427 1104 1 0.7913722168435484 1104 2 0.8880018994721759 1104 3 0.7991899472765454 1104 4 0.9397502969006979 1104 5 0.9395347818991127 1104 6 0.8762034074337635 1104 7 0.7910401746132152 1104 8 0.9123548265702226 1104 9 1.1076029802484015 1104 10-11 1.0911716229027633 1104 12-13 1.0131167012734892 1104 14-15 1.0537714324221952 1104 16-17 1.180438617272145 1104 18-19 1.4528689040162988 1104 20-21 1.7163639701266291 1104 22-23 1.4259340093498025 1104 24-25 1.8503640682975089 1104 26-27 1.8516211613912859 1104 28-29 1.6880363360440427 1104 30-31 1.603606773582328 1104 32-33 1.5306696771165278 1104 34-35 1.7912924835644617 1104 36-37 2.000435409893619 1104 38-39 2.0938420749471334 1104 40-41 1.7764242943545483 1104 42-43 1.74306689943149 1104 44-45 1.6498269813197197 1104 46-47 1.3588429908276467 1104 48-49 1.2533391171856891 1104 50-51 1.1329553860376294 1104 52-53 1.2494022159030536 1104 54-55 1.0614138053815196 1104 56-57 1.103810974649491 1104 58-59 1.0793342828692758 1104 60-61 1.0633303458315808 1104 62-63 1.1359710566181747 1104 64-65 1.1797452428968995 1104 66-67 1.0951723405439076 1104 68-69 1.086114013774523 1104 70-71 1.079021395235067 1104 72-73 0.9445793523414316 1104 74-75 1.0008289046909589 1104 76-77 1.1147350751093619 1104 78-79 1.1433261388974216 1104 80-81 1.0737868067983953 1104 82-83 1.060716285852667 1104 84-85 0.9060878620573583 1104 86-87 1.0725305390568352 1104 88-89 1.0302604014566086 1104 90-91 1.1592243503270616 1104 92-93 1.212816509933944 1104 94-95 1.219168531580106 1104 96-97 1.0530138967845986 1104 98-99 1.0901566746216567 1104 100-101 1.215355291206599 1104 102-103 1.172474976939462 1104 104-105 1.2473347213095884 1104 106-107 1.1475995016415936 1104 108-109 1.199726744653173 1104 110-111 1.2242405085507926 1104 112-113 1.1922763982210043 1104 114-115 1.2806905752526134 1104 116-117 1.2128825050107963 1104 118-119 1.17360742812809 1104 120-121 1.179189752811837 1104 122-123 1.1830857374840562 1104 124-125 1.4193801031323616 1105 1 -0.11518484241472038 1105 2 0.3086011458707638 1105 3 0.3782522668019297 1105 4 0.35421815172136917 1105 5 0.44265055623870353 1105 6 0.3035527578261181 1105 7 0.3664722030772154 1105 8 0.14466437663059395 1105 9 0.24175493634081846 1105 10-11 0.515270713815525 1105 12-13 0.46737317614739027 1105 14-15 0.6336016903671222 1105 16-17 0.4457652788501889 1105 18-19 0.6784844865227342 1105 20-21 0.8659292156124963 1105 22-23 1.1042823056694289 1105 24-25 1.228188761061567 1105 26-27 0.9626212785104755 1105 28-29 0.7137985194688454 1105 30-31 0.714283399418818 1105 32-33 0.6920367003909433 1105 34-35 1.3369818915471399 1105 36-37 1.3554505344236318 1105 38-39 1.4779776747387388 1105 40-41 1.0467285215496247 1105 42-43 0.6499236222749829 1105 44-45 0.7116089700035815 1105 46-47 0.6567186910186749 1105 48-49 0.5401094949152707 1105 50-51 0.5120263885375138 1105 52-53 0.539104612000159 1105 54-55 0.38407535918971547 1105 56-57 0.2930364129377807 1105 58-59 0.3396950907493732 1105 60-61 0.41233866937133 1105 62-63 0.3966486921028647 1105 64-65 0.5384837480521583 1105 66-67 0.3634540404683868 1105 68-69 0.15039710297492448 1105 70-71 0.29831597423196143 1105 72-73 0.41381779489850956 1105 74-75 0.3905306949991463 1105 76-77 0.47088443842520533 1105 78-79 0.46728670607739176 1105 80-81 0.45158847958352766 1105 82-83 0.37918355911597246 1105 84-85 0.2824879412137804 1105 86-87 0.3562745972771886 1105 88-89 0.49468362982691616 1105 90-91 0.3706145955100304 1105 92-93 0.3268324462211041 1105 94-95 0.3286028862735577 1105 96-97 0.4630245586695736 1105 98-99 0.302417034621822 1105 100-101 0.28559005460563824 1105 102-103 0.21798668683926437 1105 104-105 0.26525173630488297 1105 106-107 0.2496111247116488 1105 108-109 0.2105701643720046 1105 110-111 0.297779244713567 1105 112-113 0.35859352468146 1105 114-115 0.40613471949499313 1105 116-117 0.377576521027855 1105 118-119 0.33619390449626607 1105 120-121 0.2797755506964208 1105 122-123 0.38113327979841216 1105 124-125 0.4898123537189676 1106 1 0.36894302971315085 1106 2 0.7529248221944371 1106 3 0.4959319204815813 1106 4 0.5226763601795739 1106 5 0.5201277457158966 1106 6 0.41197100624436445 1106 7 0.4476443642493777 1106 8 0.533564809531029 1106 9 0.7162417828276659 1106 10-11 0.7937342502790656 1106 12-13 0.8608736756478876 1106 14-15 1.112161796927225 1106 16-17 0.8917885888734958 1106 18-19 0.8738784259166721 1106 20-21 1.3052359089191867 1106 22-23 1.3691394485265747 1106 24-25 1.5418138026866046 1106 26-27 1.6506465865357853 1106 28-29 1.5179996516699745 1106 30-31 1.3651125702479874 1106 32-33 1.2863031006573422 1106 34-35 1.5070997736650256 1106 36-37 1.542336315309413 1106 38-39 1.745822496583557 1106 40-41 1.4249489677700709 1106 42-43 1.305282929634295 1106 44-45 1.3652726396672534 1106 46-47 1.232068008367989 1106 48-49 1.2862526850584572 1106 50-51 1.2404120029231294 1106 52-53 1.257937112832657 1106 54-55 1.0000447231590783 1106 56-57 0.9967393766407397 1106 58-59 0.9935672186215072 1106 60-61 1.2091884862211444 1106 62-63 1.079459880914051 1106 64-65 1.1365463521147632 1106 66-67 0.9590051560194972 1106 68-69 0.9946308692086951 1106 70-71 1.2064578323738218 1106 72-73 1.1768867259674387 1106 74-75 1.1837255001939475 1106 76-77 0.9854964930372603 1106 78-79 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-2.7389367658551365 2216 96-97 -2.726421155433677 2216 98-99 -2.710758146361581 2216 100-101 -2.6210642499938714 2216 102-103 -2.560911583839829 2216 104-105 -2.691107771766955 2216 106-107 -2.675185197687412 2216 108-109 -2.7306093182458326 2216 110-111 -2.8437327820724363 2216 112-113 -2.7350978296263335 2216 114-115 -2.8130172472597152 2216 116-117 -2.9095818685277948 2216 118-119 -3.015084611508282 2216 120-121 -3.1493448722596185 2216 122-123 -3.226874579921777 2216 124-125 -3.435902114461257 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 79536.0 3 14937.0 4 10049.0 5 8416.0 6 8119.0 7 8170.0 8 8342.0 9 9044.0 10 10251.0 11 11217.0 12 12401.0 13 14534.0 14 18122.0 15 15864.0 16 10845.0 17 9507.0 18 8728.0 19 8313.0 20 8132.0 21 8632.0 22 10123.0 23 13937.0 24 18639.0 25 22946.0 26 27815.0 27 31408.0 28 35813.0 29 43030.0 30 48593.0 31 55701.0 32 69831.0 33 89184.0 34 117125.0 35 163924.0 36 256671.0 37 377527.0 38 150803.0 39 537.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.222698519563636 19.427224769467987 13.02864371594867 22.321432995019705 2 14.789051809267562 21.117992934449347 41.135475163235355 22.95748009304773 3 19.05279080853575 27.67358676062758 28.869914930955026 24.403707499881648 4 11.824396108407086 17.053187378415245 38.331716790503755 32.790699722673914 5 12.74445848231366 40.14973727164378 33.39812773359977 13.707676512442783 6 29.935835935508976 37.503144352907306 18.4989230763303 14.062096635253413 7 28.045008441422254 34.2369994665968 20.865423306912717 16.852568785068232 8 26.13269953312645 35.72953258702552 20.01039209232229 18.127375787525747 9 27.765172657126406 16.895265208435852 19.88245862603084 35.4571035084069 10-11 24.42692124357237 28.207209954391484 27.08686754375632 20.279001258279823 12-13 25.567420419951798 25.84104714652901 27.422285963873676 21.16924646964552 14-15 22.67335404766204 26.663789268773918 25.944283814196435 24.718572869367602 16-17 21.639133056024313 30.30251642794416 26.27268047338907 21.785670042642455 18-19 21.550535196412994 28.800065174210275 28.809753232494963 20.83964639688177 20-21 22.997376193518182 27.769467237648936 28.337275144078163 20.895881424754716 22-23 22.44271300633556 27.904036373043876 28.073516703087414 21.57973391753315 24-25 21.951496768653183 27.824565942595424 28.605594028338967 21.618343260412423 26-27 22.01840014071376 28.08277228126126 28.271354042368824 21.627473535656154 28-29 21.529312104308886 28.030535712516468 28.736683697003123 21.70346848617152 30-31 21.845295334112386 27.987674619194063 28.55968854084158 21.607341505851966 32-33 21.70911537004906 27.95028030016118 28.247975365292984 22.09262896449678 34-35 22.048479847496203 27.778657009854342 28.437989321321215 21.734873821328236 36-37 22.328202988367682 27.507697168906137 28.25756904331608 21.9065307994101 38-39 22.09205808563128 27.728667313236816 28.388576184274694 21.790698416857207 40-41 22.09361286049573 27.745102411260365 28.43621256440791 21.725072163835996 42-43 21.96309437404303 27.86621648661942 28.139445719257306 22.031243420080244 44-45 21.905914567480945 27.984671604372807 27.978065486447 22.131348341699248 46-47 22.11842584733401 28.016489256882544 27.52961692036871 22.335467975414737 48-49 22.164585629017132 28.104565087951933 27.331205819986828 22.399643463044114 50-51 22.2353565450303 28.3066154602386 27.200625970510252 22.257402024220855 52-53 22.319687840921727 28.28151781792482 27.250565014977163 22.148229326176295 54-55 21.98889166594182 28.170510560569546 27.47900603036432 22.36159174312432 56-57 22.16492930126948 28.45693053978739 27.070285891216844 22.307854267726288 58-59 22.23821832468975 28.007686202155263 27.228552776863452 22.525542696291534 60-61 22.32849302852991 28.078731098866623 27.393198619467274 22.199577253136198 62-63 22.33148097221293 27.96355068639766 27.510191564806075 22.194776776583335 64-65 22.21792822193155 27.957134866841947 27.39660267505208 22.428334236174422 66-67 22.032837543687663 28.339438161335345 27.188827848253855 22.438896446723138 68-69 22.25834022646163 28.242410562520558 27.295742042393773 22.20350716862404 70-71 22.02282691561417 28.171039486105414 27.34661187913349 22.45952171914692 72-73 22.064901967746366 28.284258717181633 27.10527887577168 22.54556043930032 74-75 22.110316964494583 28.5165390130259 27.004208029106678 22.368935993372833 76-77 22.283323246159785 28.16903075335636 27.205616229973245 22.34202977051061 78-79 22.21110346008172 28.28665379752514 27.082442318669226 22.419800423723917 80-81 22.105909787849605 28.587088778512104 27.125625410598797 22.181376023039494 82-83 22.252477931588334 28.541005109710433 26.929021647051833 22.277495311649403 84-85 22.166971420645257 28.332212293713116 26.94356345286074 22.557252832780886 86-87 22.32332912599929 28.06562450286761 27.13133212489452 22.479714246238583 88-89 22.550519751664268 27.951643070367755 27.048666592347615 22.449170585620365 90-91 22.118403044253323 28.281177225796434 27.222080305834705 22.378339424115534 92-93 22.18763546289607 28.33671173495717 27.06439605050401 22.411256751642757 94-95 21.979095553744852 28.16536919993834 27.12785461031954 22.72768063599727 96-97 22.333430132936595 28.05489225653638 26.922997521287883 22.688680089239142 98-99 22.091515643736244 28.12231405595543 26.80564932039337 22.980520979914953 100-101 22.089858038633263 28.26084802498705 27.14581082129954 22.50348311508014 102-103 22.2019511593678 28.04582428336946 27.111884524479212 22.64034003278353 104-105 21.98134516184342 27.95225845408301 27.30951393443367 22.75688244963989 106-107 21.936766725470825 28.216720379355614 27.145695783439244 22.70081711173432 108-109 22.018025436407328 28.368953404015706 26.67244983668783 22.940571322889134 110-111 21.906378078705206 28.240146987429227 26.971565770599653 22.881909163265913 112-113 22.047913710406295 28.248739793677334 26.958012204103337 22.745334291813034 114-115 21.891179161212836 28.51286296639193 26.829377035897856 22.76658083649738 116-117 21.72327091105844 28.61571055380825 26.872172861006867 22.788845674126442 118-119 21.70400590940165 28.740107421649235 26.841844244112888 22.71404242483622 120-121 21.51468598597728 28.75788626603536 26.90335464225993 22.82407310572743 122-123 21.816073176182293 28.599335302399982 26.722676448150175 22.861915073267554 124-125 21.781785468697784 28.73056325877134 26.5447677501086 22.942883522422278 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2001.0 1 1891.5 2 2334.0 3 2690.5 4 2116.5 5 1463.5 6 1123.0 7 1205.0 8 1609.0 9 1773.5 10 1434.0 11 1135.0 12 1179.5 13 1276.5 14 1324.5 15 1517.5 16 1700.5 17 1837.0 18 2073.0 19 2359.5 20 2700.5 21 3236.5 22 4029.5 23 4870.5 24 5815.5 25 7347.0 26 9797.5 27 12798.0 28 15551.0 29 18594.5 30 22751.5 31 26958.0 32 31368.5 33 35942.0 34 40689.5 35 44216.0 36 48314.5 37 53333.5 38 55592.0 39 56060.0 40 56131.0 41 57585.0 42 59280.5 43 61790.5 44 65014.0 45 67116.0 46 67155.5 47 66160.0 48 69760.0 49 70420.0 50 65489.5 51 60925.5 52 56758.5 53 55084.0 54 53612.0 55 50035.0 56 45651.5 57 41196.5 58 37931.0 59 34223.5 60 29456.0 61 25163.5 62 20880.0 63 15845.0 64 11515.0 65 9049.0 66 6948.5 67 5353.5 68 4070.5 69 2917.0 70 2185.5 71 1762.0 72 1553.5 73 1447.0 74 1162.0 75 890.0 76 662.5 77 441.0 78 301.0 79 204.5 80 154.0 81 114.0 82 91.5 83 69.0 84 48.5 85 32.0 86 24.5 87 18.5 88 12.0 89 12.0 90 9.5 91 7.5 92 7.0 93 4.5 94 4.5 95 4.5 96 2.5 97 2.5 98 4.0 99 2.5 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011393872408444456 2 0.003522743160098769 3 0.006825314872691365 4 0.007265657767703711 5 0.013815758331012359 6 9.357286519012355E-4 7 0.00710052918207408 8 0.0 9 0.002421885922567904 10-11 0.0 12-13 0.0031649645579012375 14-15 0.017063287181728413 16-17 0.027576473800148178 18-19 0.005311636171086424 20-21 0.007183093474888896 22-23 0.0019815430275555573 24-25 0.010237972309037047 26-27 0.017200894336419772 28-29 4.403428950123461E-4 30-31 0.001486157270666668 32-33 0.007816086386469143 34-35 0.008559165021802479 36-37 0.010403100894666676 38-39 0.0 40-41 0.02273270195501237 42-43 0.008779336469308651 44-45 0.014751486982913595 46-47 0.003742914607604942 48-49 0.02226483762906175 50-51 0.003687871745728399 52-53 0.0 54-55 0.01607251566795063 56-57 0.0043208646573086465 58-59 2.2017144750617306E-4 60-61 0.004183257502617288 62-63 0.02397116634723459 64-65 0.015301915601679029 66-67 0.0018989787347407426 68-69 0.018907223054592612 70-71 0.0028071859557037065 72-73 0.010760879496864209 74-75 5.5042861876543265E-5 76-77 0.005641893342345685 78-79 9.357286519012355E-4 80-81 0.003275050281654324 82-83 0.0015962429944197548 84-85 0.0 86-87 0.005339157602024696 88-89 0.015054222723234582 90-91 0.019815430275555577 92-93 0.0043208646573086465 94-95 0.022347401921876564 96-97 0.016430294270148166 98-99 9.082072209629639E-4 100-101 0.007981214972098773 102-103 0.0 104-105 0.0014035929778518533 106-107 5.5042861876543265E-5 108-109 0.0 110-111 6.605143425185191E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 0.0 122-123 0.0 124-125 0.023393216297530887 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1816766.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.13114369787831 #Duplication Level Percentage of deduplicated Percentage of total 1 72.31559539393805 34.8063231350326 2 12.95835192326533 12.474005970127227 3 5.427436443194747 7.836861700755236 4 2.7941144991656874 5.379357058706759 5 1.677838512649266 4.037814327707812 6 1.0876786443227922 3.141073027620827 7 0.7582650623397907 2.554731527358999 8 0.5244566142769789 2.0194157332054403 9 0.3767006083373172 1.6317927999865434 >10 1.9046329059464795 16.01221520441209 >50 0.11385179226472927 3.7640411684870285 >100 0.058493828656304925 4.901772142430345 >500 0.0018791066491734899 0.6013930946107052 >1k 7.046649934400588E-4 0.8392031095582791 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4154 0.22864804823516072 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2976 0.16380755694459276 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2507 0.13799245472449398 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 1882 0.10359066605165443 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.5042861876543265E-5 0.0 0.0 0.0 0.0 8 5.5042861876543265E-5 0.0 0.0 0.0 0.0 9 5.5042861876543265E-5 0.0 0.0 0.0 0.0 10-11 5.5042861876543265E-5 5.5042861876543265E-5 0.0 8.25642928148149E-5 0.0 12-13 5.5042861876543265E-5 5.5042861876543265E-5 0.0 1.1008572375308653E-4 0.0 14-15 1.1008572375308653E-4 5.5042861876543265E-5 0.0 1.1008572375308653E-4 0.0 16-17 1.3760715469135815E-4 5.5042861876543265E-5 0.0 1.651285856296298E-4 0.0 18-19 2.2017144750617303E-4 5.5042861876543265E-5 0.0 2.752143093827163E-4 5.5042861876543265E-5 20-21 2.752143093827163E-4 1.1008572375308653E-4 0.0 4.128214640740745E-4 5.5042861876543265E-5 22-23 3.302571712592596E-4 1.1008572375308653E-4 0.0 6.054714806419759E-4 5.5042861876543265E-5 24-25 3.8530003313580285E-4 1.651285856296298E-4 0.0 0.0012109429612839518 5.5042861876543265E-5 26-27 3.8530003313580285E-4 2.2017144750617306E-4 0.0 0.0020641073203703725 5.5042861876543265E-5 28-29 3.8530003313580285E-4 2.2017144750617306E-4 0.0 0.003770436038543214 5.5042861876543265E-5 30-31 3.8530003313580285E-4 3.302571712592596E-4 0.0 0.008696772176493835 5.5042861876543265E-5 32-33 3.8530003313580285E-4 3.8530003313580285E-4 0.0 0.01687063716516051 5.5042861876543265E-5 34-35 4.4034289501234606E-4 3.8530003313580285E-4 0.0 0.02898006677800003 5.5042861876543265E-5 36-37 5.504286187654326E-4 3.8530003313580285E-4 0.0 0.05022661146234573 5.5042861876543265E-5 38-39 6.054714806419759E-4 3.8530003313580285E-4 0.0 0.08451831441143218 5.5042861876543265E-5 40-41 6.054714806419759E-4 3.8530003313580285E-4 0.0 0.12401156780785197 5.5042861876543265E-5 42-43 6.054714806419759E-4 4.128214640740745E-4 0.0 0.16146823531483967 5.5042861876543265E-5 44-45 6.054714806419759E-4 4.403428950123461E-4 0.0 0.20379619609790145 5.5042861876543265E-5 46-47 6.605143425185191E-4 4.403428950123461E-4 0.0 0.25633460775906197 5.5042861876543265E-5 48-49 6.605143425185191E-4 4.403428950123461E-4 0.0 0.31823030593923485 5.5042861876543265E-5 50-51 6.605143425185191E-4 4.403428950123461E-4 0.0 0.3891255120362226 5.5042861876543265E-5 52-53 6.605143425185191E-4 4.953857568888893E-4 0.0 0.4537458318792844 5.5042861876543265E-5 54-55 6.605143425185191E-4 6.054714806419759E-4 0.0 0.5208155590758523 5.5042861876543265E-5 56-57 6.605143425185191E-4 6.054714806419759E-4 0.0 0.6029945518575315 5.5042861876543265E-5 58-59 6.605143425185191E-4 6.054714806419759E-4 0.0 0.7041082891247414 5.5042861876543265E-5 60-61 6.605143425185191E-4 6.054714806419759E-4 0.0 0.8193955633251613 5.5042861876543265E-5 62-63 6.605143425185191E-4 6.054714806419759E-4 0.0 0.9291235084760503 5.5042861876543265E-5 64-65 7.155572043950624E-4 6.329929115802475E-4 0.0 1.0376955535275318 5.5042861876543265E-5 66-67 7.155572043950624E-4 7.43078635333334E-4 0.0 1.161514471318816 5.5042861876543265E-5 68-69 7.155572043950624E-4 7.706000662716057E-4 0.0 1.3116989199489644 5.5042861876543265E-5 70-71 7.43078635333334E-4 7.706000662716057E-4 0.0 1.4842032490700507 5.5042861876543265E-5 72-73 7.981214972098774E-4 8.25642928148149E-4 0.0 1.661413742881582 5.5042861876543265E-5 74-75 8.25642928148149E-4 8.806857900246922E-4 0.0 1.8388994510024956 5.5042861876543265E-5 76-77 8.806857900246922E-4 8.806857900246922E-4 0.0 2.0277239886699774 5.5042861876543265E-5 78-79 8.806857900246922E-4 8.806857900246922E-4 0.0 2.246904664662373 5.5042861876543265E-5 80-81 0.0010733358065925936 8.806857900246922E-4 0.0 2.4982854148525453 5.5042861876543265E-5 82-83 0.0011559000994074086 8.806857900246922E-4 0.0 2.7589133658379783 5.5042861876543265E-5 84-85 0.0012109429612839518 8.806857900246922E-4 0.0 3.031458096419682 5.5042861876543265E-5 86-87 0.0012935072540987667 8.806857900246922E-4 0.0 3.307140270128349 5.5042861876543265E-5 88-89 0.0013210286850370383 8.806857900246922E-4 0.0 3.623664247349411 5.5042861876543265E-5 90-91 0.0014861572706666682 8.806857900246922E-4 0.0 3.9849105498451642 5.5042861876543265E-5 92-93 0.0015412001325432114 8.806857900246922E-4 0.0 4.3775588050414855 5.5042861876543265E-5 94-95 0.0015412001325432114 9.357286519012355E-4 0.0 4.767730131453362 5.5042861876543265E-5 96-97 0.0015412001325432114 9.357286519012355E-4 0.0 5.144911342462375 5.5042861876543265E-5 98-99 0.0015687215634814832 9.907715137777787E-4 0.0 5.568053343138302 5.5042861876543265E-5 100-101 0.0016237644253580263 9.907715137777787E-4 0.0 6.055512927916968 5.5042861876543265E-5 102-103 0.001651285856296298 9.907715137777787E-4 0.0 6.581034651683266 5.5042861876543265E-5 104-105 0.0019265001656790142 9.907715137777787E-4 0.0 7.1063637254329945 5.5042861876543265E-5 106-107 0.0020641073203703725 9.907715137777787E-4 0.0 7.626133470133193 5.5042861876543265E-5 108-109 0.002091628751308644 0.0011008572375308652 0.0 8.168223095324329 5.5042861876543265E-5 110-111 0.002091628751308644 0.0011008572375308652 0.0 8.781538183783713 5.5042861876543265E-5 112-113 0.0021466716131851873 0.0011008572375308652 0.0 9.454272041638824 5.5042861876543265E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 13710 0.0 36.71725 1 GTATCAA 10620 0.0 36.138695 1 TACATGG 13835 0.0 35.783386 2 GGTATCA 7030 0.0 33.43328 1 ACATGGG 14375 0.0 33.36295 3 ATCAACG 12195 0.0 30.64186 3 TCAACGC 12300 0.0 30.477034 4 CAACGCA 12380 0.0 30.328156 5 AACGCAG 12565 0.0 29.781986 6 CATGGGA 10035 0.0 29.529034 4 TATCAAC 12940 0.0 29.429506 2 CATGGGG 4590 0.0 27.482767 4 ATGGGAG 3995 0.0 27.405537 5 ACGCAGA 14175 0.0 26.06358 7 CGCAGAG 14130 0.0 25.432917 8 ATGGGAT 3650 0.0 25.431326 5 AGTACAT 9850 0.0 23.619886 2 GAGTACT 4685 0.0 23.369932 12-13 GCAGAGT 14905 0.0 23.153112 9 TAGACGC 130 0.0020520582 22.885685 4 >>END_MODULE