FastQCFastQC Report
Thu 26 May 2016
SRR1364529_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364529_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1816766
Sequences flagged as poor quality0
Sequence length125
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97840.5385393606000993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81490.448544281431951No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54380.29932308288464227No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21470.1181770244489384No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82650.045.1458441
GTATCAA130800.042.425621
ATCAACG161900.033.839223
TCAACGC164600.033.2841384
TATCAAC165400.033.1591572
CAACGCA166250.032.923875
AACGCAG168950.032.3272826
GTACATG132300.031.8526731
TACATGG135300.030.3249972
CGTATAG1254.825522E-528.6106361
GAGTACT100450.028.48917412-13
ACATGGG139150.028.2876993
ACGCAGA191200.028.1608267
CGCAGAG190850.028.1501268
GTACTTT109050.026.35155114-15
GCAGAGT200450.026.2973739
AGTACTT102650.025.96592312-13
CATGGGA98000.025.6428174
ATGGGAG37600.023.734945
CATGGGG44200.023.5773564
Produced by FastQC (version 0.11.5)