##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364529_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1816766 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.231258731173966 31.0 30.0 34.0 26.0 34.0 2 30.131875541484153 31.0 30.0 34.0 26.0 34.0 3 30.370039399680532 31.0 30.0 34.0 26.0 34.0 4 33.92784211065156 35.0 35.0 37.0 30.0 37.0 5 33.35846443625651 35.0 35.0 37.0 27.0 37.0 6 33.385251044988735 36.0 35.0 37.0 27.0 37.0 7 33.17656484104172 36.0 33.0 37.0 26.0 37.0 8 33.104586391423 36.0 33.0 37.0 26.0 37.0 9 34.32368395269396 38.0 34.0 39.0 25.0 39.0 10-11 34.276458002846816 38.0 34.0 39.0 25.0 39.0 12-13 34.16154639617871 38.0 34.0 39.0 25.0 39.0 14-15 35.120239205269144 39.0 34.0 41.0 25.0 41.0 16-17 35.14978263573845 39.0 34.0 41.0 25.0 41.0 18-19 35.21699052051832 39.0 34.0 41.0 25.0 41.0 20-21 35.25407922649367 39.0 34.0 41.0 25.0 41.0 22-23 35.21689116815264 39.0 34.0 41.0 25.0 41.0 24-25 35.17132090759074 39.0 34.0 41.0 25.0 41.0 26-27 35.042471622652556 39.0 34.0 41.0 25.0 41.0 28-29 34.89512766090955 39.0 34.0 41.0 24.0 41.0 30-31 34.70102836578844 38.5 34.0 41.0 24.0 41.0 32-33 34.44824980212091 38.0 33.0 41.0 23.5 41.0 34-35 34.24019549022824 38.0 33.0 40.5 22.5 41.0 36-37 34.004267473081285 38.0 33.0 40.0 20.5 41.0 38-39 33.82020359253751 38.0 33.0 40.0 18.5 41.0 40-41 33.81465114384571 38.0 33.0 40.0 18.0 41.0 42-43 33.65333812940136 38.0 33.0 41.0 15.5 41.0 44-45 33.43353574428407 38.0 33.0 40.5 14.0 41.0 46-47 33.100105076823326 38.0 32.0 40.0 8.5 41.0 48-49 32.75370906324755 38.0 32.0 40.0 4.0 41.0 50-51 32.569357308536155 38.0 31.0 40.0 2.0 41.0 52-53 32.39034223449801 38.0 31.0 40.0 2.0 41.0 54-55 32.201971250012384 37.5 31.0 40.0 2.0 41.0 56-57 31.989737258403117 37.0 31.0 40.0 2.0 41.0 58-59 31.78494451129094 37.0 30.5 40.0 2.0 41.0 60-61 31.552513642373313 36.5 30.0 40.0 2.0 41.0 62-63 31.376577390814226 36.0 30.0 40.0 2.0 41.0 64-65 31.236292125678265 36.0 30.0 39.5 2.0 41.0 66-67 30.946209088016836 35.0 30.0 39.0 2.0 41.0 68-69 30.66800402473406 35.0 30.0 39.0 2.0 41.0 70-71 30.35705093556352 35.0 29.0 38.5 2.0 40.5 72-73 30.06393393535546 35.0 29.0 37.5 2.0 40.0 74-75 29.709595512025214 35.0 29.0 37.0 2.0 39.5 76-77 29.39514554983966 35.0 29.0 36.5 2.0 39.0 78-79 29.10056551036292 35.0 29.0 36.0 2.0 39.0 80-81 28.814092183583355 34.0 29.0 36.0 2.0 37.5 82-83 28.521527538494226 34.0 28.5 35.0 2.0 37.0 84-85 28.28745061279218 34.0 28.0 35.0 2.0 37.0 86-87 28.061459758714108 34.0 28.0 35.0 2.0 36.0 88-89 27.87000472267755 34.0 27.5 35.0 2.0 36.0 90-91 27.719724224253426 34.0 27.0 35.0 2.0 36.0 92-93 27.60840168739397 34.0 27.0 35.0 2.0 35.0 94-95 27.493697867529445 34.0 27.0 35.0 2.0 35.0 96-97 27.37193535105787 34.0 27.0 35.0 2.0 35.0 98-99 27.281591850574042 34.0 27.0 35.0 2.0 35.0 100-101 27.17653374182476 34.0 26.0 35.0 2.0 35.0 102-103 25.90518481741732 31.5 24.5 34.0 2.0 35.0 104-105 26.825272214473408 33.5 25.0 35.0 2.0 35.0 106-107 26.98536190131255 34.0 25.0 35.0 2.0 35.0 108-109 26.983339626567208 34.0 25.0 35.0 2.0 35.0 110-111 26.899428159707966 34.0 25.0 35.0 2.0 35.0 112-113 26.797843255543093 34.0 25.0 35.0 2.0 35.0 114-115 26.685185929283133 34.0 25.0 35.0 2.0 35.0 116-117 26.557069815265148 34.0 25.0 35.0 2.0 35.0 118-119 26.42470439231029 34.0 25.0 35.0 2.0 35.0 120-121 26.225640781476535 34.0 24.5 35.0 2.0 35.0 122-123 25.93559764988997 34.0 23.5 35.0 2.0 35.0 124-125 24.129513652281034 31.5 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5352663487563731 1101 2 0.7085305959514905 1101 3 0.47486765802081266 1101 4 0.45734160996799744 1101 5 0.621140198144218 1101 6 0.7562736571337751 1101 7 0.7671063539043388 1101 8 0.7942023617197478 1101 9 0.9500844289990695 1101 10-11 0.7462653532824959 1101 12-13 0.9219012633532415 1101 14-15 0.9754149884144496 1101 16-17 0.9525250375189103 1101 18-19 1.106595865959413 1101 20-21 1.0392041615222922 1101 22-23 1.0182891078416034 1101 24-25 1.0527104136723082 1101 26-27 1.010828337025636 1101 28-29 1.1877160331953363 1101 30-31 1.1335605758148901 1101 32-33 1.2009069541844823 1101 34-35 1.1582016637596908 1101 36-37 1.1481392883155692 1101 38-39 1.1572573339496657 1101 40-41 1.0015181979486698 1101 42-43 1.0091854921173535 1101 44-45 0.8971999739528655 1101 46-47 1.0163218156453055 1101 48-49 0.9984595746073026 1101 50-51 1.0252954427667333 1101 52-53 1.0597476769649816 1101 54-55 0.964046951593275 1101 56-57 1.0099040688983862 1101 58-59 1.074316596813322 1101 60-61 1.0413317262391253 1101 62-63 1.0555938677289909 1101 64-65 0.9087745776271632 1101 66-67 0.9641044439668214 1101 68-69 0.9892028666255896 1101 70-71 0.8148941287087403 1101 72-73 0.8092427113651119 1101 74-75 0.874688345568984 1101 76-77 0.8507403510582137 1101 78-79 0.826831236274316 1101 80-81 0.6558477263639624 1101 82-83 0.7044113409386554 1101 84-85 0.7689894516442664 1101 86-87 0.7501780341909274 1101 88-89 0.747753461618192 1101 90-91 0.7011518223570548 1101 92-93 0.6003440763355563 1101 94-95 0.6248171109761884 1101 96-97 0.6817385793082806 1101 98-99 0.6793354219254581 1101 100-101 0.7435779474634963 1101 102-103 0.6292322496679397 1101 104-105 0.7528987241239946 1101 106-107 0.7114314915244506 1101 108-109 0.6503894779640298 1101 110-111 0.5696644283585286 1101 112-113 0.4622639505255748 1101 114-115 0.5768114042921617 1101 116-117 0.6913742344988627 1101 118-119 0.7305690776746232 1101 120-121 0.6667647520003612 1101 122-123 0.635296797185628 1101 124-125 0.9363126090062472 1102 1 0.12882625579183227 1102 2 -0.5352627075368694 1102 3 0.08663489641210376 1102 4 0.029342500209274647 1102 5 0.2126341616516214 1102 6 -0.06838223177578584 1102 7 0.03546695999811078 1102 8 0.18098227875218242 1102 9 0.4079187346655999 1102 10-11 0.5537244076565386 1102 12-13 0.5617723243092243 1102 14-15 0.648189804802179 1102 16-17 0.728137534463265 1102 18-19 0.6983453724957585 1102 20-21 0.5924048343111963 1102 22-23 0.7840879352041128 1102 24-25 0.6692637934879144 1102 26-27 0.6909910614558257 1102 28-29 0.44734271562365535 1102 30-31 0.7082304385936808 1102 32-33 0.7196420818647837 1102 34-35 0.7734381432202895 1102 36-37 0.5815124635945921 1102 38-39 0.17295557134490736 1102 40-41 -0.20189784755579154 1102 42-43 0.019046154763344703 1102 44-45 -0.33022398156425936 1102 46-47 -0.08371780738859513 1102 48-49 -0.27962290405792345 1102 50-51 -0.48772360620748145 1102 52-53 -0.6950214258250504 1102 54-55 -0.5656165445009762 1102 56-57 -0.6557617330667114 1102 58-59 -0.5129364615667988 1102 60-61 -0.7440653979896688 1102 62-63 -0.7153500434220135 1102 64-65 -0.6308124817672507 1102 66-67 -0.7417986583780909 1102 68-69 -0.5479784251728823 1102 70-71 -1.1939062711701283 1102 72-73 -0.957803256462153 1102 74-75 -0.9164719963712358 1102 76-77 -1.2444121392458172 1102 78-79 -1.0834559395082017 1102 80-81 -1.0536037171768626 1102 82-83 -1.2255194464505692 1102 84-85 -1.0863389707679225 1102 86-87 -0.8281842867118634 1102 88-89 -0.9960766190508039 1102 90-91 -0.9548302706585687 1102 92-93 -0.8619278631864127 1102 94-95 -0.7387944227562926 1102 96-97 -1.0807554827094314 1102 98-99 -1.1558419513519773 1102 100-101 -1.016581493289852 1102 102-103 -0.9809859689008853 1102 104-105 -0.9500571687989527 1102 106-107 -0.8841377338561855 1102 108-109 -0.832969942357547 1102 110-111 -0.7915947522546425 1102 112-113 -0.6062430345847751 1102 114-115 -0.5958541021420203 1102 116-117 -0.6574147819669562 1102 118-119 -0.525254450234808 1102 120-121 -0.3257620373298771 1102 122-123 -0.433497983957313 1102 124-125 -0.1752257206238319 1103 1 0.3582522530678496 1103 2 0.08655026997935522 1103 3 0.3127071911848276 1103 4 0.3935865223798629 1103 5 0.4335911970269706 1103 6 0.5507809085238478 1103 7 0.6261140877994791 1103 8 0.6393985818570869 1103 9 0.9243442002552982 1103 10-11 0.9554105087934701 1103 12-13 0.8423035736264097 1103 14-15 0.9441885374950658 1103 16-17 0.7579385526789792 1103 18-19 0.9759115601591759 1103 20-21 0.9571547596566035 1103 22-23 1.0250815331181613 1103 24-25 1.029877126432794 1103 26-27 1.0609073026520477 1103 28-29 1.071361972865148 1103 30-31 1.1549327452383054 1103 32-33 1.1243759837868623 1103 34-35 1.0120081255216888 1103 36-37 1.1396945333838886 1103 38-39 1.222294168130027 1103 40-41 1.1084896279538654 1103 42-43 1.1559869190806324 1103 44-45 1.1628103449785456 1103 46-47 1.1709215563358342 1103 48-49 1.1824182615732894 1103 50-51 1.149918692716767 1103 52-53 1.1454354602411243 1103 54-55 1.1819346394115087 1103 56-57 1.2691961326133026 1103 58-59 1.3481258185663485 1103 60-61 1.1145970831646004 1103 62-63 1.1705967588125255 1103 64-65 1.1484733833883567 1103 66-67 1.202557236420855 1103 68-69 1.154242858303327 1103 70-71 1.0740808955244567 1103 72-73 1.1021700863881243 1103 74-75 1.0820680512620342 1103 76-77 1.0998204480272165 1103 78-79 1.0116301135584607 1103 80-81 0.9611596095771588 1103 82-83 1.0929053782021967 1103 84-85 1.1810642149799726 1103 86-87 1.20658173489063 1103 88-89 1.1752614096311227 1103 90-91 1.1476149037418288 1103 92-93 1.131610982690109 1103 94-95 0.9626980862932975 1103 96-97 0.9580283394597053 1103 98-99 1.038687943257596 1103 100-101 0.9797847584672645 1103 102-103 0.9473752718947495 1103 104-105 0.8248995315411811 1103 106-107 0.8767778591197306 1103 108-109 0.8888237342169347 1103 110-111 0.8598047481058835 1103 112-113 0.7509248981751142 1103 114-115 0.8268204257132439 1103 116-117 0.8757640467207999 1103 118-119 0.8964029544076162 1103 120-121 0.9855760641857927 1103 122-123 1.022409852225035 1103 124-125 1.2142747855706801 1104 1 0.2577819866589124 1104 2 0.4327516218608771 1104 3 0.2272242713920356 1104 4 -0.1654635212776867 1104 5 0.25066329856944236 1104 6 0.24134384694313127 1104 7 0.3541609010463418 1104 8 0.29237122413562844 1104 9 0.23399424231377708 1104 10-11 0.39638362417816353 1104 12-13 0.43513562949043205 1104 14-15 0.5592089546721226 1104 16-17 0.40301720106214134 1104 18-19 0.36060089677116736 1104 20-21 0.29097760346017054 1104 22-23 0.3509988724309352 1104 24-25 0.39615674760601394 1104 26-27 0.35063953449721197 1104 28-29 0.5337656112609679 1104 30-31 0.501711418958557 1104 32-33 0.4264200725378231 1104 34-35 0.579912918699037 1104 36-37 0.6953040807541839 1104 38-39 0.8260035298695811 1104 40-41 0.6335395094981209 1104 42-43 0.6495644893276591 1104 44-45 0.69301290664432 1104 46-47 0.5909101670496995 1104 48-49 0.7249227548410389 1104 50-51 0.5032252525430465 1104 52-53 0.6124207980860632 1104 54-55 0.25444125834848563 1104 56-57 0.3947889282763768 1104 58-59 0.5157736428708155 1104 60-61 0.6096541440119516 1104 62-63 0.5911221630412022 1104 64-65 0.5302167657609687 1104 66-67 0.5538073973624087 1104 68-69 0.32950401096745807 1104 70-71 -0.003223091442514203 1104 72-73 0.014069092010533524 1104 74-75 0.4323846133132392 1104 76-77 0.4259224393464365 1104 78-79 0.4385863564780692 1104 80-81 0.4555675969102637 1104 82-83 0.4847131564973779 1104 84-85 0.5238942030723628 1104 86-87 0.6082328643212769 1104 88-89 0.523877780923506 1104 90-91 0.6411543580651653 1104 92-93 0.5771139653817556 1104 94-95 0.5193368325289178 1104 96-97 0.5755504156788689 1104 98-99 0.5813588921414556 1104 100-101 0.5621109491659873 1104 102-103 0.4364838362464205 1104 104-105 0.4031926715295988 1104 106-107 0.4452650057134342 1104 108-109 0.3958833133344264 1104 110-111 0.44550754094852607 1104 112-113 0.49693540852045004 1104 114-115 0.43248527908204437 1104 116-117 0.5055965324056153 1104 118-119 0.43246447805754684 1104 120-121 0.43042204690528507 1104 122-123 0.3885201918275776 1104 124-125 0.8343522828942938 1105 1 -0.05487657859061201 1105 2 -0.2548224358344626 1105 3 -0.004844174678616753 1105 4 -0.009398564551212019 1105 5 -0.40636082394530604 1105 6 0.08827269905390267 1105 7 0.13691651368067426 1105 8 0.11802840414782167 1105 9 0.28290362033010297 1105 10-11 0.09854285781479177 1105 12-13 0.15349002408517975 1105 14-15 0.3307827135115957 1105 16-17 0.256886778746491 1105 18-19 0.26070569825414225 1105 20-21 0.31482206109734534 1105 22-23 0.30814213343307273 1105 24-25 0.2719577015216288 1105 26-27 0.2913210874169181 1105 28-29 0.3272182446253922 1105 30-31 0.408139041317817 1105 32-33 0.5066814502633719 1105 34-35 0.5411404085924545 1105 36-37 0.6279966913409538 1105 38-39 0.6134687474891507 1105 40-41 0.45828174631311924 1105 42-43 0.3692374844692381 1105 44-45 0.5235802077739109 1105 46-47 0.4765592294903911 1105 48-49 0.4529970326396793 1105 50-51 0.3755112172215149 1105 52-53 0.29188031321260866 1105 54-55 0.10000385451748173 1105 56-57 0.2560075332656666 1105 58-59 0.10089546702241137 1105 60-61 0.20037608285958086 1105 62-63 0.32495224328804184 1105 64-65 0.1594012527183324 1105 66-67 0.34229821562169604 1105 68-69 0.4015300921997138 1105 70-71 0.44368112525221903 1105 72-73 0.42962090513676543 1105 74-75 0.4277525493096235 1105 76-77 0.34632864100952787 1105 78-79 0.22331421590273237 1105 80-81 0.27488378237859834 1105 82-83 0.25932211975021957 1105 84-85 0.23084300583153095 1105 86-87 0.3474883901258643 1105 88-89 0.21055910768201258 1105 90-91 0.1521532636065217 1105 92-93 0.16453253725457273 1105 94-95 0.19504106214715122 1105 96-97 0.24412733673649356 1105 98-99 0.23738184750288838 1105 100-101 0.3013206369423749 1105 102-103 0.05924554715587149 1105 104-105 0.06204425728305907 1105 106-107 0.10873007794366885 1105 108-109 0.1899451684216409 1105 110-111 0.08914958431493503 1105 112-113 0.03195889293753851 1105 114-115 0.10323334762503933 1105 116-117 -0.09728924407442818 1105 118-119 0.04549984401463192 1105 120-121 0.0886413909570436 1105 122-123 0.0716334218537078 1105 124-125 0.6380528454702734 1106 1 -0.1261040178180508 1106 2 -0.4637428487548725 1106 3 0.0319976501632091 1106 4 -0.08378018293282707 1106 5 0.2058309842465036 1106 6 0.3453582801394788 1106 7 0.3954846122487723 1106 8 0.30191784803726307 1106 9 0.5457580991845816 1106 10-11 0.4546440899160231 1106 12-13 0.45877673737189895 1106 14-15 0.36256226729114616 1106 16-17 0.2153749572346726 1106 18-19 0.383724013272456 1106 20-21 0.3522812685565597 1106 22-23 0.29601026530119867 1106 24-25 0.4165471121110329 1106 26-27 0.5100237181195482 1106 28-29 0.4450037924109438 1106 30-31 0.393383130561908 1106 32-33 0.407320810902732 1106 34-35 0.5645343479863953 1106 36-37 0.5815777769220389 1106 38-39 0.5623518643722676 1106 40-41 0.521230131261504 1106 42-43 0.5607972580290053 1106 44-45 0.42701344120714424 1106 46-47 0.3452671881983491 1106 48-49 0.5685041921468397 1106 50-51 0.7545941343044333 1106 52-53 0.6808373561696541 1106 54-55 0.6719439144575432 1106 56-57 0.1938869871451132 1106 58-59 0.6484845446114953 1106 60-61 0.636420038903541 1106 62-63 0.48568084801664213 1106 64-65 0.6801778094948858 1106 66-67 0.554224289547772 1106 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-1.1281580339444446 2216 108-109 -1.1294787866187548 2216 110-111 -1.172068763547248 2216 112-113 -1.0796340802992646 2216 114-115 -1.1327619356305192 2216 116-117 -1.204524588638538 2216 118-119 -1.3210295170352744 2216 120-121 -1.409295147390452 2216 122-123 -1.2802750577945012 2216 124-125 -1.4729036637739092 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 51456.0 3 18446.0 4 8961.0 5 7236.0 6 8212.0 7 9851.0 8 10917.0 9 12175.0 10 13541.0 11 15002.0 12 16781.0 13 20287.0 14 24801.0 15 21207.0 16 13033.0 17 11420.0 18 10444.0 19 9705.0 20 9691.0 21 9929.0 22 10813.0 23 13110.0 24 17269.0 25 22849.0 26 28009.0 27 30677.0 28 35111.0 29 41704.0 30 47713.0 31 56009.0 32 68542.0 33 85410.0 34 111217.0 35 154789.0 36 244791.0 37 386457.0 38 158641.0 39 560.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.469311408174995 19.712973334222212 14.010456065845997 22.8072591917568 2 15.055607081818506 21.204778823898522 39.966864178911415 23.772749915371552 3 18.81898934700451 27.019935423714447 29.922070316155192 24.23900491312585 4 12.173014670431847 17.298546731868587 37.61416075487293 32.91427784282664 5 12.42388948274021 39.86996674310285 33.59144766029307 14.114696113863868 6 30.047457955509955 37.729349844724084 17.777027971681548 14.44616422808441 7 27.23004307201145 34.45271023407505 20.9424651502016 17.374781543711897 8 26.379596775170178 35.186557264068995 19.728467831518827 18.705378129242 9 27.447520113436703 15.77451593442161 20.09598744093673 36.68197651120496 10-11 24.62148890068647 27.63171619311669 26.891275743337097 20.855519162859746 12-13 25.974478826662324 25.181751529916347 27.83311114364756 21.010658499773776 14-15 22.994470941481147 25.68650871191376 26.876178261910592 24.4428420846945 16-17 21.268553613516794 29.286802564122322 27.338253442112947 22.106390380247937 18-19 21.1190420163673 26.99910416090426 31.00954598434277 20.872307838385673 20-21 22.435549532570356 26.03578159608618 30.775910089698932 20.752758781644527 22-23 21.892032325571922 26.17067910782126 30.629563741285338 21.30772482532148 24-25 21.486724212143997 26.187935045019557 31.050201291745882 21.275139451090563 26-27 21.55115188197049 26.4910010425118 30.750520430259044 21.20732664525866 28-29 21.086480042008713 26.402547163476196 31.22234784226477 21.288624952250316 30-31 21.358694515419156 26.34491728709146 31.12139923358319 21.174988963906195 32-33 21.250617856124563 26.287232367844844 30.811480399787317 21.650669376243282 34-35 21.510255035596217 26.285223303386346 30.8167920359584 21.387729625059034 36-37 21.872354502451056 25.983753548888522 30.574823615149114 21.569068333511304 38-39 21.7302888759477 26.23554712054277 30.612170196932354 21.42199380657718 40-41 21.66726479909906 26.175990743992344 30.72828311406092 21.428461342847676 42-43 21.691511179755675 26.255059815078003 30.33673571610213 21.716693289064196 44-45 21.575178091179602 26.51301818726242 29.963022205391336 21.948781516166637 46-47 21.81279812590064 26.5903809296299 29.357330553301857 22.239490391167603 48-49 21.995980770225778 26.80854331267758 28.827598050602006 22.36787786649464 50-51 22.090571928360614 27.001853843588002 28.619178254106476 22.288395973944912 52-53 22.15054112637511 27.108444345611925 28.533421475302816 22.207593052710145 54-55 21.8614560157995 26.992303907052424 28.73292432817435 22.413315748973726 56-57 22.045432964564927 27.223915679148092 28.290449499279575 22.440201857007402 58-59 22.048031819387763 26.828948773471804 28.521775650418274 22.601243756722152 60-61 22.17231057824728 26.88153014752588 28.673505558778622 22.27265371544822 62-63 22.269681400906887 26.737482977994965 28.791600018934744 22.201235602163404 64-65 22.083073599328475 26.727599232150595 28.7085406045094 22.48078656401153 66-67 21.91930606363175 27.054942683867928 28.566915056754695 22.458836195745626 68-69 22.138685482523748 26.989091327416777 28.562629574372693 22.30959361568678 70-71 21.90895800559896 26.942600202777907 28.579519872124425 22.568921919498713 72-73 22.03162102329084 26.965442990456665 28.23759361414734 22.765342372105156 74-75 22.05428446322529 27.227796754028976 28.1608393475252 22.55707943522053 76-77 22.13457869643091 26.914693471806494 28.411418971953463 22.539308859809136 78-79 21.95943230993975 27.005679322488422 28.301470855355067 22.733417512216764 80-81 21.819592418167687 27.225908263263694 28.322776100803402 22.631723217765213 82-83 22.03602445224096 27.16767046499109 28.086968822622175 22.709336260145776 84-85 21.895114725837008 27.011046001521382 28.012110530470075 23.081728742171528 86-87 21.988302290993996 26.757518579718027 28.209411668866547 23.04476746042143 88-89 22.18402202401053 26.632556201491187 28.17449013881699 23.008931635681297 90-91 21.730674175980834 26.974635148390053 28.322634835746598 22.972055839882515 92-93 21.727214605814794 27.06345499616481 28.16576610098867 23.043564297031725 94-95 21.58233366322355 26.82893669300284 28.198953525110003 23.3897761186636 96-97 21.826283259496737 26.753857705281884 28.011382652656795 23.40847638256458 98-99 21.584521384928717 26.796746848681675 27.882699400011006 23.7360323663786 100-101 21.559116169288963 26.936641038174123 28.176947787450846 23.327295005086064 102-103 21.751232684891725 26.666505207605162 28.162817886288057 23.41944422121506 104-105 21.504392970806364 26.521522309422345 28.29547118341052 23.678613536360764 106-107 21.498803348422665 26.81427020766914 28.082561252708153 23.604365191200042 108-109 21.470816825061675 26.820487613704792 27.69465082459711 24.01404473663642 110-111 21.32931336744158 26.63228320668881 28.015098265323253 24.023305160546357 112-113 21.44131385109585 26.59935291611578 27.981754392145163 23.97757884064321 114-115 21.27761637987501 26.72694226994561 27.81491947779736 24.180521872382023 116-117 21.01038328546439 26.791067204031783 27.974020870051508 24.22452864045232 118-119 20.93381866459412 26.863778824570694 27.88135621208235 24.321046298752837 120-121 20.776435729617248 26.809322468880563 27.96327839183201 24.45096340967018 122-123 21.020547517286232 26.721983341695605 27.632759882456654 24.624709258561513 124-125 20.76469047396729 26.602538942264392 27.660803395172156 24.97196718859616 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 871.0 1 648.0 2 684.0 3 992.0 4 741.0 5 547.5 6 1082.5 7 2475.0 8 4087.0 9 4586.0 10 3372.5 11 1995.5 12 1593.0 13 1534.5 14 1682.0 15 1880.0 16 2026.0 17 2334.0 18 2728.0 19 3014.5 20 3378.5 21 3989.5 22 4813.0 23 5644.5 24 6530.0 25 7963.5 26 10427.5 27 13269.5 28 15712.5 29 18797.0 30 22971.0 31 26943.0 32 31062.5 33 35635.0 34 40225.5 35 43690.0 36 47586.0 37 52528.5 38 54786.5 39 55299.5 40 55604.5 41 56665.0 42 58606.0 43 60983.5 44 63932.0 45 66630.0 46 66936.0 47 65378.5 48 69491.0 49 70646.5 50 65840.5 51 61456.0 52 56770.0 53 55575.5 54 54125.0 55 50412.5 56 45975.5 57 41071.5 58 37574.5 59 34048.5 60 29665.0 61 24840.0 62 20287.0 63 15543.0 64 11216.5 65 8765.0 66 6752.5 67 5114.0 68 3843.5 69 2753.0 70 2061.0 71 1715.0 72 1505.0 73 1451.0 74 1155.5 75 887.5 76 695.0 77 392.5 78 218.5 79 149.0 80 110.0 81 74.0 82 45.0 83 28.5 84 25.5 85 21.0 86 12.0 87 5.5 88 5.0 89 5.5 90 3.5 91 3.5 92 2.5 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.09593970825081491 2 5.5042861876543265E-5 3 0.0 4 0.1040860518085433 5 0.0 6 0.0 7 0.0022567573369382736 8 0.006164800530172846 9 0.0038530003313580283 10-11 5.22907187827161E-4 12-13 0.0 14-15 5.5042861876543265E-5 16-17 2.2017144750617306E-4 18-19 0.0018439358728641994 20-21 0.001210942961283952 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.007348222060518526 58-59 6.605143425185191E-4 60-61 0.0 62-63 0.0 64-65 1.9265001656790145E-4 66-67 0.0 68-69 2.7521430938271632E-5 70-71 0.0 72-73 0.0 74-75 0.0011834215303456802 76-77 0.0 78-79 0.0 80-81 1.1008572375308653E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0011834215303456802 90-91 0.0 92-93 0.0020641073203703725 94-95 0.0 96-97 0.007816086386469143 98-99 0.0036328288838518557 100-101 0.002091628751308644 102-103 0.0 104-105 0.0 106-107 0.001651285856296298 108-109 0.0 110-111 5.5042861876543265E-5 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 8.531643590864207E-4 122-123 0.004100693209802473 124-125 0.08160104273197538 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1816766.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.50720155575638 #Duplication Level Percentage of deduplicated Percentage of total 1 72.63745440014759 33.78164732284727 2 12.47502058014428 11.603565930659581 3 5.2723868081755905 7.356118679031999 4 2.847465955325242 5.2971069242986175 5 1.7546589989257801 4.08021398623315 6 1.0939737383942487 3.052659428892338 7 0.7442795798956312 2.423005230522786 8 0.5337705686138507 1.985934033524404 9 0.413366555548757 1.7302069543783332 >10 2.033089493441608 16.619307473762287 >50 0.12394509143850699 3.9785733770905987 >100 0.06655502866919615 5.374225784229971 >500 0.002846965609258353 0.8417609946923303 >1k 8.303649693670196E-4 0.5863308331098497 >5k 3.558707011572941E-4 1.2893430467265992 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9784 0.5385393606000993 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8149 0.448544281431951 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5438 0.29932308288464227 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2147 0.1181770244489384 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.5042861876543265E-5 0.0 0.0 0.0 0.0 8 5.5042861876543265E-5 0.0 0.0 0.0 0.0 9 5.5042861876543265E-5 0.0 0.0 0.0 0.0 10-11 5.5042861876543265E-5 0.0 0.0 0.0 0.0 12-13 8.25642928148149E-5 5.5042861876543265E-5 0.0 0.0 0.0 14-15 1.1008572375308653E-4 5.5042861876543265E-5 0.0 0.0 0.0 16-17 1.1008572375308653E-4 5.5042861876543265E-5 0.0 5.5042861876543265E-5 0.0 18-19 1.1008572375308653E-4 5.5042861876543265E-5 0.0 1.1008572375308653E-4 0.0 20-21 1.1008572375308653E-4 1.651285856296298E-4 0.0 1.9265001656790142E-4 0.0 22-23 1.1008572375308653E-4 1.651285856296298E-4 0.0 5.22907187827161E-4 0.0 24-25 2.2017144750617306E-4 1.9265001656790142E-4 0.0 0.001265985823160495 0.0 26-27 2.2017144750617306E-4 2.2017144750617306E-4 0.0 0.0021466716131851873 0.0 28-29 3.0273574032098794E-4 2.2017144750617306E-4 0.0 0.0038805217622963004 0.0 30-31 3.302571712592596E-4 3.0273574032098794E-4 0.0 0.009164636502444454 0.0 32-33 3.302571712592596E-4 3.302571712592596E-4 0.0 0.017806365817061746 0.0 34-35 3.302571712592596E-4 3.302571712592596E-4 0.0 0.03013596687740744 0.0 36-37 3.302571712592596E-4 3.302571712592596E-4 0.0 0.05141003299269141 0.0 38-39 3.8530003313580285E-4 4.128214640740745E-4 0.0 0.08619712169866675 0.0 40-41 3.8530003313580285E-4 4.403428950123461E-4 0.0 0.12530507506195074 0.0 42-43 3.8530003313580285E-4 4.6786432595061775E-4 0.0 0.16226635681204954 0.0 44-45 3.8530003313580285E-4 4.953857568888893E-4 0.0 0.20396132468353106 0.0 46-47 3.8530003313580285E-4 4.953857568888893E-4 0.0 0.2558117005712348 0.0 48-49 4.403428950123461E-4 4.953857568888893E-4 0.0 0.3166340629448151 0.0 50-51 4.403428950123461E-4 4.953857568888893E-4 0.0 0.3855477260142473 0.0 52-53 4.403428950123461E-4 5.229071878271609E-4 0.0 0.44837915284632146 0.0 54-55 4.953857568888893E-4 5.504286187654326E-4 0.0 0.5137700727556549 0.0 56-57 5.504286187654326E-4 5.779500497037043E-4 0.0 0.5931969224435067 0.0 58-59 5.504286187654326E-4 6.054714806419759E-4 0.0 0.6922465523903463 0.0 60-61 5.504286187654326E-4 6.054714806419759E-4 0.0 0.8067081836626181 0.0 62-63 5.504286187654326E-4 6.054714806419759E-4 0.0 0.9143169786312602 0.0 64-65 5.504286187654326E-4 7.43078635333334E-4 0.0 1.0214579092739515 0.0 66-67 6.054714806419759E-4 7.981214972098774E-4 0.0 1.1425246839714085 0.0 68-69 6.329929115802475E-4 8.25642928148149E-4 0.0 1.288470832237063 0.0 70-71 7.155572043950624E-4 8.25642928148149E-4 0.0 1.4566267752699027 0.0 72-73 7.706000662716057E-4 8.25642928148149E-4 0.0 1.6306998259544707 0.0 74-75 7.706000662716057E-4 8.25642928148149E-4 0.0 1.8041949265893351 0.0 76-77 7.706000662716057E-4 8.25642928148149E-4 0.0 1.9890563782017057 0.0 78-79 7.706000662716057E-4 8.25642928148149E-4 0.0 2.2035308895036563 0.0 80-81 8.531643590864205E-4 8.25642928148149E-4 0.0 2.4510035965005947 0.0 82-83 9.357286519012355E-4 8.25642928148149E-4 0.0 2.7068978613646446 0.0 84-85 0.0010182929447160502 8.25642928148149E-4 0.0 2.9751767701509166 0.0 86-87 0.0011008572375308652 8.25642928148149E-4 0.0 3.2463179077547686 0.0 88-89 0.0011008572375308652 8.25642928148149E-4 0.0 3.5569247773241024 0.0 90-91 0.0012935072540987667 8.25642928148149E-4 0.0 3.9101073005549423 0.0 92-93 0.0013210286850370383 8.25642928148149E-4 0.0 4.295599983707313 0.0 94-95 0.0013210286850370383 8.25642928148149E-4 0.0 4.6780102665945975 0.0 96-97 0.0013210286850370383 8.25642928148149E-4 0.0 5.049026677073437 0.0 98-99 0.0014035929778518533 8.25642928148149E-4 0.0 5.467214820180475 0.0 100-101 0.001568721563481483 8.25642928148149E-4 0.0 5.949004990185857 0.0 102-103 0.001706328718172841 8.25642928148149E-4 0.0 6.467013363306006 0.0 104-105 0.0018164144419259276 8.25642928148149E-4 0.0 6.987443622348723 0.0 106-107 0.001871457303802471 8.25642928148149E-4 0.0 7.50038805217623 0.0 108-109 0.001871457303802471 8.25642928148149E-4 0.0 8.03623031254438 0.0 110-111 0.001871457303802471 8.25642928148149E-4 0.0 8.644151200539861 0.0 112-113 0.001871457303802471 8.806857900246922E-4 0.0 9.310747779295738 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8265 0.0 45.145844 1 GTATCAA 13080 0.0 42.42562 1 ATCAACG 16190 0.0 33.83922 3 TCAACGC 16460 0.0 33.284138 4 TATCAAC 16540 0.0 33.159157 2 CAACGCA 16625 0.0 32.92387 5 AACGCAG 16895 0.0 32.327282 6 GTACATG 13230 0.0 31.852673 1 TACATGG 13530 0.0 30.324997 2 CGTATAG 125 4.825522E-5 28.610636 1 GAGTACT 10045 0.0 28.489174 12-13 ACATGGG 13915 0.0 28.287699 3 ACGCAGA 19120 0.0 28.160826 7 CGCAGAG 19085 0.0 28.150126 8 GTACTTT 10905 0.0 26.351551 14-15 GCAGAGT 20045 0.0 26.297373 9 AGTACTT 10265 0.0 25.965923 12-13 CATGGGA 9800 0.0 25.642817 4 ATGGGAG 3760 0.0 23.73494 5 CATGGGG 4420 0.0 23.577356 4 >>END_MODULE