FastQCFastQC Report
Thu 26 May 2016
SRR1364528_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364528_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404379
Sequences flagged as poor quality0
Sequence length125
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27450.11416669335408436No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32250.051.104411
GTATCAA55150.047.9011341
ATCAACG61900.042.388423
TCAACGC62900.041.6199264
CAACGCA64200.040.962515
AACGCAG67350.039.395116
TATCAAC67050.038.511482
GTACATG89200.034.2851941
ACGCAGA79750.033.045947
TACATGG87150.032.974562
CGCAGAG80100.032.1615228
ACATGGG89650.031.4577243
GCAGAGT92250.026.4428189
CATGGGA56450.023.1877174
GAGTACT44950.023.16898512-13
CATGGGG45500.022.4913924
CAGAGTA89150.022.42724610-11
ATAGCGC1350.002556691922.036143
AGAGTAC83900.021.98658210-11
GTGTAGC20250.020.8613131
Produced by FastQC (version 0.11.5)