FastQCFastQC Report
Thu 26 May 2016
SRR1364528_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364528_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2404379
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43020.1789235390926306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37780.15712996994234268No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37800.057.27081
GTATCAA64100.049.031031
ATCAACG77400.040.0975233
CAACGCA79650.039.1427355
TCAACGC79450.039.0629124
TATCAAC82000.037.848152
AACGCAG83250.037.2356646
TACATGG79450.031.3403022
GTACATG83550.031.1213511
CGCAGAG98150.030.976788
ACGCAGA98050.030.887017
ACATGGG83900.028.045033
GCAGAGT112500.025.7027959
GCACCGC5150.025.4168136
TGGTATC21100.024.8439712
GTGGTAT22650.024.4868641
AGAGTAC104950.023.07300210-11
GAGTACT70600.022.96409812-13
CATGGGG40050.022.3105134
CAGAGTA109900.022.25031910-11
Produced by FastQC (version 0.11.5)