##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364528_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2404379 Sequences flagged as poor quality 0 Sequence length 125 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.95341167095537 31.0 30.0 34.0 26.0 34.0 2 29.878113225909892 31.0 30.0 34.0 26.0 34.0 3 30.12999073773311 31.0 30.0 34.0 26.0 34.0 4 33.69311285783148 35.0 35.0 37.0 28.0 37.0 5 33.08396970693888 35.0 33.0 37.0 26.0 37.0 6 33.112096304284805 35.0 33.0 37.0 26.0 37.0 7 32.89822070480569 35.0 33.0 37.0 26.0 37.0 8 32.831378081408964 35.0 33.0 37.0 25.0 37.0 9 34.09052108673383 38.0 34.0 39.0 25.0 39.0 10-11 34.01100346492795 38.0 34.0 39.0 25.0 39.0 12-13 33.8883040069806 38.0 33.5 39.0 24.0 39.0 14-15 34.85145124791058 39.0 34.0 41.0 24.0 41.0 16-17 34.84637654878869 39.0 34.0 41.0 24.0 41.0 18-19 34.85196385428421 39.0 34.0 41.0 24.0 41.0 20-21 34.85860922924381 39.0 34.0 41.0 24.0 41.0 22-23 34.80997692127572 39.0 34.0 41.0 24.0 41.0 24-25 34.77153206711587 39.0 34.0 41.0 24.0 41.0 26-27 34.64925309196262 39.0 34.0 41.0 23.0 41.0 28-29 34.504969890354225 39.0 33.5 41.0 22.5 41.0 30-31 34.374275644563525 38.5 33.0 41.0 22.0 41.0 32-33 34.19829569298351 38.0 33.0 40.5 21.0 41.0 34-35 34.031359656693056 38.0 33.0 40.0 19.0 41.0 36-37 33.84742359669586 38.0 33.0 40.0 18.0 41.0 38-39 33.69353791561147 38.0 33.0 40.0 16.5 41.0 40-41 33.74414037886706 38.0 33.0 40.0 16.0 41.0 42-43 33.638321994993305 38.0 33.0 40.0 15.5 41.0 44-45 33.50030673200855 38.0 33.0 40.0 15.0 41.0 46-47 33.30943270590868 38.0 32.0 40.0 11.0 41.0 48-49 33.08162440280837 38.0 32.0 40.0 8.5 41.0 50-51 32.93091105853112 38.0 32.0 40.0 8.0 41.0 52-53 32.755802225855405 38.0 32.0 40.0 7.0 41.0 54-55 32.58022528894155 38.0 31.0 40.0 2.0 41.0 56-57 32.369992418000656 37.5 31.0 40.0 2.0 41.0 58-59 32.175411821514 37.0 31.0 40.0 2.0 41.0 60-61 31.95180148387588 37.0 31.0 40.0 2.0 41.0 62-63 31.785739477844384 36.5 31.0 40.0 2.0 41.0 64-65 31.658874911151695 36.0 31.0 40.0 2.0 41.0 66-67 31.370279186434416 36.0 30.0 39.0 2.0 41.0 68-69 31.088417840947706 35.0 30.0 39.0 2.0 41.0 70-71 30.777812898881585 35.0 30.0 39.0 2.0 40.5 72-73 30.486040054417376 35.0 30.0 38.0 2.0 40.0 74-75 30.12619807443003 35.0 29.0 37.0 2.0 39.5 76-77 29.7968375617987 35.0 29.0 37.0 2.0 39.0 78-79 29.49702272395492 35.0 29.0 36.0 2.0 39.0 80-81 29.20083023516675 34.0 29.0 36.0 2.0 37.5 82-83 28.895354060237594 34.0 29.0 35.5 2.0 37.0 84-85 28.655166885087585 34.0 29.0 35.0 2.0 37.0 86-87 28.428342203953704 34.0 29.0 35.0 2.0 36.0 88-89 28.24063593967507 34.0 28.5 35.0 2.0 36.0 90-91 28.087783997447993 34.0 28.0 35.0 2.0 36.0 92-93 27.96300354478225 34.0 28.0 35.0 2.0 35.5 94-95 27.843300910546965 34.0 28.0 35.0 2.0 35.0 96-97 27.7237663030662 34.0 27.0 35.0 2.0 35.0 98-99 27.638110921780633 34.0 27.0 35.0 2.0 35.0 100-101 27.526697746070816 34.0 27.0 35.0 2.0 35.0 102-103 26.260430239991283 31.5 24.5 34.0 2.0 35.0 104-105 27.197846096642834 33.5 26.5 35.0 2.0 35.0 106-107 27.363704099894402 34.0 27.0 35.0 2.0 35.0 108-109 27.36560583834745 34.0 27.0 35.0 2.0 35.0 110-111 27.287298716217368 34.0 27.0 35.0 2.0 35.0 112-113 27.19902748277206 34.0 27.0 35.0 2.0 35.0 114-115 27.096119829694068 34.0 26.5 35.0 2.0 35.0 116-117 26.96971317749822 34.0 25.5 35.0 2.0 35.0 118-119 26.83312946918934 34.0 25.0 35.0 2.0 35.0 120-121 26.6350194790422 34.0 25.0 35.0 2.0 35.0 122-123 26.333779325139673 34.0 25.0 35.0 2.0 35.0 124-125 24.516912475113116 31.5 19.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8216477275107685 1101 2 0.9746692799657062 1101 3 0.8441047928691319 1101 4 0.7594427333195668 1101 5 0.9209361497366757 1101 6 0.8834699670431121 1101 7 1.0493417088461712 1101 8 1.0900293090516797 1101 9 1.3805958551232607 1101 10-11 1.278302248508325 1101 12-13 1.2295226124305998 1101 14-15 1.4315118610844877 1101 16-17 1.364933674492491 1101 18-19 1.4456484066762272 1101 20-21 1.4630772919188786 1101 22-23 1.5383837083141714 1101 24-25 1.5300683935084152 1101 26-27 1.4193825550339838 1101 28-29 1.5904508087883684 1101 30-31 1.5402426777481963 1101 32-33 1.5160751086463478 1101 34-35 1.5873240595040414 1101 36-37 1.6826718678914574 1101 38-39 1.5777185601538122 1101 40-41 1.42952752565688 1101 42-43 1.4436658845393069 1101 44-45 1.3648306074692655 1101 46-47 1.384431224872145 1101 48-49 1.329135697182224 1101 50-51 1.4282353985891802 1101 52-53 1.4303126806680737 1101 54-55 1.544787955103402 1101 56-57 1.472967677270475 1101 58-59 1.569280121496675 1101 60-61 1.4509679689277704 1101 62-63 1.5229496790481853 1101 64-65 1.3971633372199932 1101 66-67 1.6661914718378554 1101 68-69 1.6663877886291836 1101 70-71 1.5893702054506385 1101 72-73 1.5081070295142105 1101 74-75 1.5894747632274822 1101 76-77 1.4973378621713707 1101 78-79 1.558625455904238 1101 80-81 1.2565257598804003 1101 82-83 1.4629962318876402 1101 84-85 1.4507594558355699 1101 86-87 1.5025405813726813 1101 88-89 1.4422875861438094 1101 90-91 1.426827497750594 1101 92-93 1.4204078539304987 1101 94-95 1.423240711265553 1101 96-97 1.467624886059312 1101 98-99 1.465951226554072 1101 100-101 1.4581718177154492 1101 102-103 1.3697949656375172 1101 104-105 1.4861109929033027 1101 106-107 1.505488567847376 1101 108-109 1.4738002823637153 1101 110-111 1.3667391694491862 1101 112-113 1.433340441346573 1101 114-115 1.47353862768081 1101 116-117 1.5022016141543304 1101 118-119 1.5326031534022349 1101 120-121 1.6060052551596549 1101 122-123 1.5294350577912041 1101 124-125 1.788175582111208 1102 1 0.45925434319576297 1102 2 -0.1343634646677927 1102 3 0.3375968968691261 1102 4 0.4302535945475725 1102 5 0.5989565956886267 1102 6 0.5496155324578638 1102 7 0.500481515579267 1102 8 0.5225319548422647 1102 9 0.9978162780612436 1102 10-11 1.0383434630822777 1102 12-13 1.0917316403411164 1102 14-15 1.258264477699825 1102 16-17 1.2600862613913364 1102 18-19 1.263039588204677 1102 20-21 1.218002792357737 1102 22-23 1.2884618920376951 1102 24-25 1.2308326662826445 1102 26-27 1.3355879167247124 1102 28-29 0.9496786605517258 1102 30-31 1.257632242960689 1102 32-33 1.3859977449436656 1102 34-35 1.3431808322608418 1102 36-37 1.0058310730851403 1102 38-39 0.5512877905990976 1102 40-41 0.2542372078787878 1102 42-43 0.6059213926549134 1102 44-45 0.2812975454298652 1102 46-47 0.5067517919291689 1102 48-49 0.1717281730683453 1102 50-51 0.10418421683237966 1102 52-53 -0.00823650671796372 1102 54-55 0.1287702862491713 1102 56-57 0.200896326856288 1102 58-59 0.2197077927664779 1102 60-61 0.17820764530276278 1102 62-63 0.13611277822874612 1102 64-65 0.13704705292573394 1102 66-67 0.001479941974039889 1102 68-69 0.16238121570983566 1102 70-71 -0.3426998904651093 1102 72-73 -0.144650991231277 1102 74-75 0.030323679057609354 1102 76-77 -0.29310473555383254 1102 78-79 -0.1022653773748452 1102 80-81 -0.17414043958692815 1102 82-83 -0.36265154380210873 1102 84-85 -0.346542586609484 1102 86-87 -0.1015765609036059 1102 88-89 -0.1525242472463404 1102 90-91 -0.15085364789415223 1102 92-93 -0.0277328711369762 1102 94-95 0.17967605840499346 1102 96-97 -0.0727164324185452 1102 98-99 -0.2012409034404392 1102 100-101 0.007540112506564611 1102 102-103 -0.018036154850534558 1102 104-105 0.19912797860454035 1102 106-107 0.19429043435124527 1102 108-109 0.13590765289792017 1102 110-111 0.28927652302986573 1102 112-113 0.3966479932047342 1102 114-115 0.3028692311701846 1102 116-117 0.22293936303547568 1102 118-119 0.40665910996396804 1102 120-121 0.6850128549586394 1102 122-123 0.6868332769669117 1102 124-125 0.8743027571127016 1103 1 0.4396780109083167 1103 2 0.06321502985307248 1103 3 0.2447134889897562 1103 4 0.32625456121405705 1103 5 0.39749080153544014 1103 6 0.45031808760736425 1103 7 0.5769896347909764 1103 8 0.5598603184727438 1103 9 0.8813553075916261 1103 10-11 0.8152406017227634 1103 12-13 0.7837367726394859 1103 14-15 0.8511114054980595 1103 16-17 0.7121377779458768 1103 18-19 0.9185553616276678 1103 20-21 0.9683674447251889 1103 22-23 1.007731784221427 1103 24-25 0.7845317539317733 1103 26-27 0.8594000577410483 1103 28-29 0.9043583016085108 1103 30-31 0.8901855309701503 1103 32-33 0.9369368157117606 1103 34-35 0.9270832746523681 1103 36-37 0.9730686573511562 1103 38-39 0.9922342954362122 1103 40-41 0.8669828509398556 1103 42-43 0.9863837745288535 1103 44-45 1.0932430611854755 1103 46-47 1.075635611294949 1103 48-49 0.9345321619926494 1103 50-51 0.9637823673998795 1103 52-53 0.9905808409671195 1103 54-55 0.9596900088432037 1103 56-57 1.0817638862742882 1103 58-59 1.084065202260625 1103 60-61 1.1305393496625484 1103 62-63 1.0910601749166382 1103 64-65 1.0489151106698031 1103 66-67 1.0062498888593367 1103 68-69 1.0868686353144916 1103 70-71 0.9405793326034235 1103 72-73 0.8546565181136323 1103 74-75 0.9599716013704018 1103 76-77 0.9951073515476878 1103 78-79 0.8409274914410005 1103 80-81 0.9803814279022767 1103 82-83 0.9890883222688629 1103 84-85 0.9835078486798992 1103 86-87 0.9606354487102102 1103 88-89 1.0357120065172687 1103 90-91 1.0140690507025951 1103 92-93 0.9355995260103498 1103 94-95 0.9534851934329502 1103 96-97 0.9855329794097258 1103 98-99 1.0452974226144427 1103 100-101 0.9376331437645398 1103 102-103 0.713424949960789 1103 104-105 0.7598751069949792 1103 106-107 0.8840070075007453 1103 108-109 0.9742884613024536 1103 110-111 0.8900688347655539 1103 112-113 0.862620204809101 1103 114-115 0.9598922294305687 1103 116-117 1.045595029813427 1103 118-119 0.9791878572327875 1103 120-121 0.9415065320662066 1103 122-123 0.9658935396858404 1103 124-125 1.2806521542004994 1104 1 0.37320152650672966 1104 2 0.5428097039006197 1104 3 0.285858091895836 1104 4 0.11132313083540879 1104 5 0.41921615372285714 1104 6 0.5695499861541791 1104 7 0.5543528510787326 1104 8 0.5164423724270577 1104 9 0.5922189034656355 1104 10-11 0.6104293371026799 1104 12-13 0.5505292723264077 1104 14-15 0.722996799457654 1104 16-17 0.6870897548013772 1104 18-19 0.6465083693446232 1104 20-21 0.5802409947446137 1104 22-23 0.6148758239389593 1104 24-25 0.7045180492053049 1104 26-27 0.5553533428071447 1104 28-29 0.786542369457166 1104 30-31 0.6883304402338268 1104 32-33 0.7499128285490642 1104 34-35 0.9196485843600897 1104 36-37 0.985922410160903 1104 38-39 1.0235588993469733 1104 40-41 0.9378309096020274 1104 42-43 0.8110934600276636 1104 44-45 0.8025316394244655 1104 46-47 0.7707878738483629 1104 48-49 0.8008748537367723 1104 50-51 0.6231820621743296 1104 52-53 0.727973589277731 1104 54-55 0.32010029453404343 1104 56-57 0.503781304083077 1104 58-59 0.646104666495674 1104 60-61 0.7834197124238997 1104 62-63 0.724124472512262 1104 64-65 0.7116896916214372 1104 66-67 0.7895277877415268 1104 68-69 0.4667267202894543 1104 70-71 0.16878099938683278 1104 72-73 0.17147838001782034 1104 74-75 0.5785905724758571 1104 76-77 0.7247026269900445 1104 78-79 0.7951571365260826 1104 80-81 0.7182137250677272 1104 82-83 0.6655096481533924 1104 84-85 0.7674219878964301 1104 86-87 0.7803747829732295 1104 88-89 0.6614770337816758 1104 90-91 0.7669315131242627 1104 92-93 0.6356331687436239 1104 94-95 0.6181102662194142 1104 96-97 0.6172205781534501 1104 98-99 0.6599379901613425 1104 100-101 0.654500853179055 1104 102-103 0.5837784498309588 1104 104-105 0.6596187359244752 1104 106-107 0.7265903236766391 1104 108-109 0.6340042421145675 1104 110-111 0.7784219752138846 1104 112-113 0.7260619802397876 1104 114-115 0.6743420840699059 1104 116-117 0.6477997431921949 1104 118-119 0.5458599364527537 1104 120-121 0.5766026950965539 1104 122-123 0.7319413003391979 1104 124-125 1.2263695156196768 1105 1 0.2691506921200961 1105 2 0.08154635549197664 1105 3 0.29054822789746027 1105 4 0.33781710194276826 1105 5 0.18466577849437726 1105 6 0.48012927866696486 1105 7 0.6331705777865295 1105 8 0.7067677041947817 1105 9 0.8325064853582234 1105 10-11 0.7165808948438013 1105 12-13 0.8063978203975708 1105 14-15 0.8139893096712427 1105 16-17 0.8425666545176398 1105 18-19 0.8840667320595017 1105 20-21 0.8126407358279906 1105 22-23 0.9203416890763378 1105 24-25 0.9292976656102567 1105 26-27 0.8726766461130424 1105 28-29 0.8643620915406558 1105 30-31 0.8404492729689679 1105 32-33 0.8700558451389497 1105 34-35 1.0331999271821033 1105 36-37 1.0297270484854266 1105 38-39 1.0730550925052569 1105 40-41 0.9493652299582038 1105 42-43 1.0316963387328215 1105 44-45 0.989671233710979 1105 46-47 0.831044066147804 1105 48-49 0.9720673872514496 1105 50-51 0.6972752996141764 1105 52-53 0.7843280459980733 1105 54-55 0.7687387471728258 1105 56-57 0.7715818749245429 1105 58-59 0.5704033723701158 1105 60-61 0.5886459142514511 1105 62-63 0.7911843714446682 1105 64-65 0.5663495045298887 1105 66-67 0.8239446216867705 1105 68-69 0.9410514199007629 1105 70-71 1.022077205849964 1105 72-73 1.0198217324671965 1105 74-75 0.9477654499603538 1105 76-77 1.0268303120269096 1105 78-79 0.8639575774868611 1105 80-81 0.8617594819641639 1105 82-83 0.795772165536814 1105 84-85 0.7963357875964725 1105 86-87 0.8860999519149217 1105 88-89 0.8085260494866837 1105 90-91 0.633687301174465 1105 92-93 0.7705971558907123 1105 94-95 0.7539081648272941 1105 96-97 0.8054923167138277 1105 98-99 0.5999636635962347 1105 100-101 0.6591745165078962 1105 102-103 0.36122721999458207 1105 104-105 0.5722057938241605 1105 106-107 0.5979962979771578 1105 108-109 0.5819377488333366 1105 110-111 0.4969609124941847 1105 112-113 0.4599940670704825 1105 114-115 0.4022378514729503 1105 116-117 0.1219681636001475 1105 118-119 0.41239478653911377 1105 120-121 0.43938870979735967 1105 122-123 0.37968836315252474 1105 124-125 0.844042546440118 1106 1 0.039433450761499955 1106 2 -0.34367673028707557 1106 3 0.11920074216183707 1106 4 -0.021034647979170984 1106 5 0.2711304608763285 1106 6 0.4461580349806411 1106 7 0.5383525242693992 1106 8 0.6196280467113908 1106 9 0.6758097783184454 1106 10-11 0.5734089710045609 1106 12-13 0.509678966050167 1106 14-15 0.6243874591609853 1106 16-17 0.4594438355103847 1106 18-19 0.568504644004932 1106 20-21 0.6584820510267733 1106 22-23 0.5791621218127574 1106 24-25 0.574677383087618 1106 26-27 0.7139681370928557 1106 28-29 0.6843022563352719 1106 30-31 0.6833649451356081 1106 32-33 0.6559545010388064 1106 34-35 0.7081900113844881 1106 36-37 0.7694080036746058 1106 38-39 0.6375396376637283 1106 40-41 0.5762341593481466 1106 42-43 0.512667089954796 1106 44-45 0.42499913587148797 1106 46-47 0.36221224651419703 1106 48-49 0.5651201212724999 1106 50-51 0.5362394924639631 1106 52-53 0.42092432635461563 1106 54-55 0.5244290847876698 1106 56-57 0.10490847376377133 1106 58-59 0.44516827892847033 1106 60-61 0.4129060484254552 1106 62-63 0.33499188611024877 1106 64-65 0.4244947047045642 1106 66-67 0.33079775016561896 1106 68-69 0.47111155547650085 1106 70-71 0.5100954680815803 1106 72-73 0.6570029125765551 1106 74-75 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-2.5021146657660758 2216 100-101 -2.5388130086468905 2216 102-103 -2.442586569583014 2216 104-105 -2.3412790018362095 2216 106-107 -2.395606619342736 2216 108-109 -2.43590100115269 2216 110-111 -2.4800581424437347 2216 112-113 -2.3756713521729083 2216 114-115 -2.5385684919967133 2216 116-117 -2.6014906745706057 2216 118-119 -2.6557433557510493 2216 120-121 -2.8301840228496857 2216 122-123 -2.798288675194833 2216 124-125 -3.026953874816268 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 81624.0 3 27271.0 4 12599.0 5 9558.0 6 9943.0 7 11814.0 8 13428.0 9 14344.0 10 15759.0 11 16845.0 12 18303.0 13 20390.0 14 22375.0 15 20309.0 16 15343.0 17 13628.0 18 12565.0 19 12129.0 20 11741.0 21 12206.0 22 13806.0 23 17268.0 24 22512.0 25 29685.0 26 36738.0 27 41743.0 28 48320.0 29 56989.0 30 66111.0 31 77748.0 32 95173.0 33 117830.0 34 150327.0 35 204034.0 36 327706.0 37 520106.0 38 205442.0 39 667.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.59182506110347 19.650651749551326 14.093451781024141 24.66407140832106 2 15.149233960203446 20.54451482066679 39.96237697966918 24.343874239460582 3 19.42297782504339 24.650523066454998 29.10892999814089 26.817569110360722 4 12.77054501808317 17.281908044149 37.241216224936586 32.706330712831246 5 13.061044036734643 38.490104929380934 34.67564805714906 13.773202976735366 6 31.642307639519395 37.511931355248066 17.36502439923157 13.480736606000967 7 27.886847338153252 32.40719752346499 21.067392247393972 18.63856289098779 8 25.22591470186247 36.99556530875217 19.43864678278997 18.339873206595385 9 25.654029112140847 16.379728060977694 19.783187462072988 38.183055364808475 10-11 24.62082535652835 27.126578429998418 27.560663575901685 20.691932637571554 12-13 25.42858259866685 24.162891956717306 28.080951463974692 22.327573980641155 14-15 22.217903711915575 25.750417415560822 25.98812957277733 26.043549299746278 16-17 22.08998397710837 28.9509840925231 26.53288955850227 22.426142371866256 18-19 21.91220958367378 27.655372383598998 28.677508085937507 21.754909946789716 20-21 22.65812242198767 26.808063310316 28.850405359458232 21.683408908238096 22-23 22.23459779011545 26.83372712870974 28.55862158170571 22.373053499469094 24-25 22.017639481961872 26.874174163058317 28.7775554519483 22.33063090303151 26-27 22.04459030793398 27.18046946841575 28.54416878537036 22.230771438279906 28-29 21.94697674534672 27.154620798135404 28.775184777441492 22.123217679076383 30-31 22.256495336217792 26.93360738885176 28.707038283066023 22.102858991864426 32-33 22.03051182862602 26.949203931659692 28.64926868850543 22.371015551208856 34-35 22.24778206763576 26.98917267202883 28.615746519163576 22.147298741171838 36-37 22.21816943169109 26.937766466933873 28.5344157472678 22.309648354107235 38-39 22.11306952855602 27.138254825882274 28.606700524334972 22.14197512122673 40-41 22.345603584127126 26.96879318942646 28.494946096268514 22.190657130177897 42-43 22.22946132868404 26.91574414848907 28.429898115064223 22.42489640776267 44-45 22.3319826034082 27.052099523411243 28.263451810217937 22.35246606296262 46-47 22.31985889079883 27.305117038536768 27.852555691095287 22.522468379569112 48-49 22.367417948667825 27.26758135884567 27.86332770332797 22.501672989158532 50-51 22.428722759598216 27.440453439328827 27.70314081099527 22.427682990077688 52-53 22.537752991520886 27.48044297508837 27.511386516019314 22.47041751737143 54-55 22.446544409180085 27.321690964694 27.6898317611325 22.541932864993413 56-57 22.35785782767205 27.505984331553808 27.619827010704206 22.51633083006994 58-59 22.40390374153621 27.472986574389857 27.621425244139814 22.50168443993412 60-61 22.563060981650562 27.368293434604112 27.68120167411211 22.387443909633216 62-63 22.334727594942393 27.412005345247152 27.776091040555585 22.47717601925487 64-65 22.33482027646703 27.261217943052525 27.74234119615775 22.661620584322694 66-67 22.231187346088117 27.453783284582006 27.639881233366285 22.675148135963592 68-69 22.23669812454692 27.369083659439713 27.778877622870603 22.61534059314276 70-71 22.184127377588975 27.27870689271533 27.686067795468183 22.851097934227507 72-73 22.306425068593597 27.319361880968017 27.577307903620852 22.796905146817537 74-75 22.298787136068558 27.437742542112726 27.433728984189287 22.829741337629432 76-77 22.366315792976067 27.293637983030127 27.50936936314949 22.830676860844317 78-79 22.35433764809957 27.329426849926737 27.47383004093781 22.842405461035888 80-81 22.23169105862492 27.423989193049263 27.540505789749826 22.80381395857599 82-83 22.338345992873833 27.45563407432855 27.452223630301216 22.753796302496404 84-85 22.480752826405485 27.391043591713288 27.34038602067311 22.78781756120811 86-87 22.283882865388527 27.290976173057572 27.598643974182107 22.82649698737179 88-89 22.35228721566941 27.26880635480598 27.48150386372054 22.89740256580407 90-91 22.34884766503118 27.43612799812342 27.51542082175897 22.69960351508643 92-93 22.269216004245663 27.35259241944869 27.48603877077132 22.892152805534334 94-95 22.216485005067838 27.359268235165917 27.418701460959362 23.005545298806883 96-97 22.254947816634164 27.289208130967634 27.481603291618946 22.974240760779256 98-99 22.151350924185202 27.194627913554157 27.44534746368975 23.20867369857088 100-101 22.092996656439258 27.270691167534476 27.556322507416848 23.079989668609418 102-103 22.11737417437101 27.104420725684264 27.59080411199732 23.187400987947406 104-105 22.15609519131551 27.11623250743747 27.50269404282769 23.224978258419327 106-107 22.023244574616132 27.187580323297894 27.551878972493977 23.237296129591996 108-109 22.001710212907366 27.116024553533364 27.440328666986364 23.441936566572906 110-111 21.90957237969436 27.294607831720164 27.39211753902052 23.403702249564958 112-113 21.968354406688796 27.307550099214804 27.37070569989174 23.353389794204656 114-115 21.95300740856579 27.2295049990039 27.387757920028417 23.429729672401898 116-117 21.82363928482157 27.19047205120324 27.462413371602395 23.52347529237279 118-119 21.782526797979855 27.264358073332033 27.36779434523426 23.585320783453856 120-121 21.794426822489438 27.26612183597642 27.418283194810417 23.521168146723728 122-123 21.845482155468655 27.19497343471979 27.331148051006004 23.628396358805556 124-125 21.722137940952976 27.18560386656424 27.243776327201047 23.84848186528174 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 161.0 1 132.0 2 160.5 3 244.0 4 212.0 5 173.0 6 313.0 7 746.0 8 1224.0 9 1423.0 10 1219.0 11 899.0 12 834.5 13 949.5 14 1080.5 15 1227.0 16 1450.0 17 1721.0 18 1970.0 19 2220.5 20 2617.0 21 3223.5 22 4184.5 23 5391.5 24 6898.0 25 9173.0 26 12265.0 27 16177.0 28 20068.0 29 24393.5 30 29522.5 31 34195.5 32 38803.5 33 43483.0 34 49055.0 35 54398.0 36 59835.0 37 65126.5 38 69440.0 39 73449.0 40 77020.5 41 80062.0 42 82012.5 43 84867.0 44 88809.5 45 91300.5 46 91985.0 47 93047.5 48 94324.0 49 93823.0 50 92119.5 51 89000.0 52 85556.5 53 82204.0 54 78003.0 55 72500.0 56 65369.0 57 58990.5 58 53394.5 59 46739.0 60 39180.0 61 31330.0 62 23898.0 63 18239.0 64 13484.0 65 9450.0 66 6996.0 67 5503.0 68 4203.0 69 3030.0 70 2164.0 71 1583.5 72 1176.0 73 954.0 74 727.0 75 491.0 76 326.0 77 182.5 78 103.5 79 74.0 80 58.0 81 42.0 82 28.5 83 23.0 84 12.5 85 4.5 86 5.0 87 2.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0959083405735951 2 0.0 3 0.0 4 0.10073287114884966 5 0.0 6 0.0 7 0.0021627206027003227 8 0.006363389465637489 9 0.0037431702739044053 10-11 4.990893698539207E-4 12-13 0.0 14-15 2.079539041058003E-5 16-17 2.7034007533754034E-4 18-19 0.002100334431468583 20-21 0.001310109595866542 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.007652703671093451 58-59 7.902248356020411E-4 60-61 0.0 62-63 0.0 64-65 2.2874929451638032E-4 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0011853372534030616 76-77 0.0 78-79 0.0 80-81 2.079539041058003E-5 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0010605649109395813 90-91 0.0 92-93 0.0017468127944887226 94-95 0.0 96-97 0.008838040924496512 98-99 0.004117487301294846 100-101 0.002329083725984963 102-103 0.0 104-105 0.0 106-107 0.001372495767098282 108-109 0.0 110-111 1.2477234246348017E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 9.773833492972613E-4 122-123 0.004699758232791087 124-125 0.08482439748475594 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 2404379.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.44480219921131 #Duplication Level Percentage of deduplicated Percentage of total 1 77.57308342731886 48.44035850601843 2 12.794510559261987 15.979013622176705 3 4.306199455640397 8.06699319713448 4 1.8993761357339982 4.744246683912474 5 1.0305565400477816 3.217644964919366 6 0.6174258082677331 2.3133019481980055 7 0.37792260944347306 1.6519511815315222 8 0.2776330553105276 1.386939297826287 9 0.19735847335728948 1.1091609748020832 >10 0.8685499540642766 9.299111375813284 >50 0.04237273584257235 1.7687559214600177 >100 0.01467701839367169 1.5068893612186312 >500 6.684546347203015E-5 0.02997040952497529 >1k 2.6738185388811974E-4 0.48566255546390863 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4302 0.1789235390926306 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3778 0.15712996994234268 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 4.1590780821160055E-5 0.0 0.0 4.1590780821160055E-5 0.0 12-13 4.1590780821160055E-5 0.0 0.0 4.1590780821160055E-5 0.0 14-15 4.1590780821160055E-5 0.0 0.0 4.1590780821160055E-5 0.0 16-17 4.1590780821160055E-5 0.0 0.0 6.238617123174009E-5 0.0 18-19 6.238617123174009E-5 0.0 0.0 1.6636312328464025E-4 4.1590780821160055E-5 20-21 8.318156164232011E-5 0.0 0.0 2.079539041058003E-4 4.1590780821160055E-5 22-23 8.318156164232011E-5 0.0 0.0 2.2874929451638032E-4 4.1590780821160055E-5 24-25 1.2477234246348017E-4 0.0 0.0 3.7431702739044054E-4 4.1590780821160055E-5 26-27 1.2477234246348017E-4 0.0 0.0 4.159078082116006E-4 4.1590780821160055E-5 28-29 1.2477234246348017E-4 0.0 0.0 9.357925684761014E-4 6.238617123174009E-5 30-31 1.2477234246348017E-4 0.0 0.0 0.0026202191917330834 8.318156164232011E-5 32-33 1.455677328740602E-4 0.0 0.0 0.006321798684816329 8.318156164232011E-5 34-35 1.6636312328464022E-4 0.0 0.0 0.011978144876494097 8.318156164232011E-5 36-37 2.079539041058003E-4 0.0 0.0 0.02083698119140119 8.318156164232011E-5 38-39 2.079539041058003E-4 0.0 0.0 0.03782681515684508 8.318156164232011E-5 40-41 2.079539041058003E-4 0.0 0.0 0.058081525416750025 8.318156164232011E-5 42-43 2.2874929451638032E-4 0.0 0.0 0.07731726154653655 8.318156164232011E-5 44-45 2.4954468492696034E-4 0.0 0.0 0.09865333210779165 8.318156164232011E-5 46-47 3.7431702739044054E-4 0.0 0.0 0.12595767971688324 8.318156164232011E-5 48-49 4.159078082116006E-4 0.0 0.0 0.15671406213413108 8.318156164232011E-5 50-51 4.159078082116006E-4 0.0 0.0 0.19025702686639667 8.318156164232011E-5 52-53 4.159078082116006E-4 0.0 0.0 0.22394555933153631 8.318156164232011E-5 54-55 4.159078082116006E-4 0.0 0.0 0.26283293939932095 8.318156164232011E-5 56-57 4.159078082116006E-4 0.0 0.0 0.3063992823094861 8.318156164232011E-5 58-59 4.159078082116006E-4 0.0 0.0 0.3570568533496591 8.318156164232011E-5 60-61 4.159078082116006E-4 0.0 0.0 0.41355792909520506 8.318156164232011E-5 62-63 4.159078082116006E-4 0.0 0.0 0.47116116053251167 8.318156164232011E-5 64-65 4.159078082116006E-4 0.0 0.0 0.5324867668533122 8.318156164232011E-5 66-67 4.159078082116006E-4 0.0 0.0 0.6041684775985816 8.318156164232011E-5 68-69 4.159078082116006E-4 0.0 0.0 0.6855200448847707 8.318156164232011E-5 70-71 4.5749858903276064E-4 0.0 0.0 0.7814699762391869 8.318156164232011E-5 72-73 4.990893698539207E-4 0.0 0.0 0.8797281959291776 8.318156164232011E-5 74-75 4.990893698539207E-4 0.0 0.0 0.9784231188177903 8.318156164232011E-5 76-77 4.990893698539207E-4 0.0 0.0 1.0868710798089651 8.318156164232011E-5 78-79 4.990893698539207E-4 0.0 0.0 1.215469774107992 8.318156164232011E-5 80-81 5.822709314962408E-4 0.0 0.0 1.3601225098039869 8.318156164232011E-5 82-83 5.822709314962408E-4 0.0 0.0 1.5168989581093495 8.318156164232011E-5 84-85 6.030663219068208E-4 0.0 0.0 1.6746319943735992 8.318156164232011E-5 86-87 6.238617123174009E-4 0.0 0.0 1.8389987601788236 8.318156164232011E-5 88-89 6.446571027279809E-4 0.0 0.0 2.0294013547780945 8.318156164232011E-5 90-91 7.07043273959721E-4 0.0 0.0 2.2437602391303537 8.318156164232011E-5 92-93 7.07043273959721E-4 0.0 0.0 2.4741315740987586 8.318156164232011E-5 94-95 7.07043273959721E-4 0.0 0.0 2.7059793817863156 8.318156164232011E-5 96-97 7.07043273959721E-4 0.0 0.0 2.9457502332203034 8.318156164232011E-5 98-99 7.07043273959721E-4 0.0 0.0 3.2118480489140855 8.318156164232011E-5 100-101 7.07043273959721E-4 0.0 0.0 3.5193702823057427 8.318156164232011E-5 102-103 7.486340547808811E-4 0.0 0.0 3.847271998299769 8.318156164232011E-5 104-105 7.486340547808811E-4 0.0 0.0 4.1864656112867396 8.318156164232011E-5 106-107 7.486340547808811E-4 0.0 0.0 4.523018209691568 8.318156164232011E-5 108-109 7.486340547808811E-4 0.0 0.0 4.880095858431637 8.318156164232011E-5 110-111 7.486340547808811E-4 0.0 0.0 5.282881775294161 8.318156164232011E-5 112-113 7.486340547808811E-4 0.0 0.0 5.718586795176634 8.318156164232011E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3780 0.0 57.2708 1 GTATCAA 6410 0.0 49.03103 1 ATCAACG 7740 0.0 40.097523 3 CAACGCA 7965 0.0 39.142735 5 TCAACGC 7945 0.0 39.062912 4 TATCAAC 8200 0.0 37.84815 2 AACGCAG 8325 0.0 37.235664 6 TACATGG 7945 0.0 31.340302 2 GTACATG 8355 0.0 31.121351 1 CGCAGAG 9815 0.0 30.97678 8 ACGCAGA 9805 0.0 30.88701 7 ACATGGG 8390 0.0 28.04503 3 GCAGAGT 11250 0.0 25.702795 9 GCACCGC 515 0.0 25.416813 6 TGGTATC 2110 0.0 24.843971 2 GTGGTAT 2265 0.0 24.486864 1 AGAGTAC 10495 0.0 23.073002 10-11 GAGTACT 7060 0.0 22.964098 12-13 CATGGGG 4005 0.0 22.310513 4 CAGAGTA 10990 0.0 22.250319 10-11 >>END_MODULE