FastQCFastQC Report
Thu 26 May 2016
SRR1364527_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364527_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189487
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA79740.6703730263550589No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34800.29256309652816714No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29180.24531583783597466No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA25130.21126754642967932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24310.2043738182930961No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16240.13652944504647802No Hit
AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12380.10407848089134222No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA52550.043.365171
GGTATCA33850.039.9008521
GTACATG67550.035.2329981
TATCAAC65950.034.914932
ATCAACG65050.034.391853
AACGCAG65700.034.137856
TCAACGC65350.034.0518724
CAACGCA65900.033.9482545
TACATGG68750.033.6660272
ACATGGG73000.030.4019153
ACGCAGA74850.029.6467597
CGCAGAG74800.028.8712278
CATGGGG28950.027.7459874
GAGTACT31500.027.30120512-13
ATACCGA900.00953516626.4409876
GAACAAA16800.025.8540481
GCAGAGT78300.025.8331499
CATGGGA46050.025.7121524
AGTACTT33900.024.7539212-13
GTACTTT35700.023.75588414-15
Produced by FastQC (version 0.11.5)