##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1364527_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1189487 Sequences flagged as poor quality 0 Sequence length 125 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.993704849233325 31.0 27.0 31.0 10.0 34.0 2 27.110429958461083 31.0 27.0 33.0 10.0 34.0 3 27.31060196538508 31.0 27.0 34.0 10.0 34.0 4 30.456339581685214 35.0 30.0 37.0 10.0 37.0 5 30.6681409716962 35.0 31.0 37.0 10.0 37.0 6 30.564830048583968 35.0 31.0 37.0 8.0 37.0 7 30.392039593539064 35.0 30.0 37.0 8.0 37.0 8 30.249574816706698 35.0 30.0 37.0 8.0 37.0 9 31.14464638957803 37.0 30.0 39.0 7.0 39.0 10-11 31.16985557639554 37.0 30.0 39.0 4.5 39.0 12-13 31.07163214057825 37.0 30.0 39.0 2.0 39.0 14-15 31.68033194141676 38.0 30.0 40.0 2.0 41.0 16-17 31.7875210069551 38.0 30.0 40.0 2.0 41.0 18-19 31.608640951939783 38.0 30.0 40.0 2.0 41.0 20-21 31.3222704409548 37.5 29.0 40.0 2.0 41.0 22-23 31.142752295737573 37.0 28.5 40.0 2.0 41.0 24-25 30.961202182117162 37.0 27.0 40.0 2.0 41.0 26-27 30.799925514108182 37.0 27.0 40.0 2.0 41.0 28-29 30.78521118768007 37.0 27.5 40.0 2.0 41.0 30-31 30.683120538517866 37.0 27.5 40.0 2.0 41.0 32-33 30.583843707413365 36.5 28.0 40.0 2.0 41.0 34-35 30.214925005485558 36.0 27.0 40.0 2.0 41.0 36-37 29.992987313018133 36.0 26.0 40.0 2.0 41.0 38-39 29.78625197248898 36.0 25.5 40.0 2.0 41.0 40-41 29.87546984540394 36.0 26.0 40.0 2.0 41.0 42-43 29.703900084658343 36.0 26.0 40.0 2.0 41.0 44-45 29.515974953908703 35.0 25.5 40.0 2.0 41.0 46-47 29.3719447963702 35.0 25.0 40.0 2.0 41.0 48-49 29.14560142313451 35.0 25.0 40.0 2.0 41.0 50-51 28.952831346622535 35.0 25.0 40.0 2.0 41.0 52-53 28.853281708837507 35.0 25.0 39.5 2.0 41.0 54-55 28.83710961111807 35.0 25.0 40.0 2.0 41.0 56-57 28.6813454035227 35.0 25.0 39.0 2.0 41.0 58-59 28.456123101807755 35.0 24.5 39.0 2.0 41.0 60-61 28.232109724612375 34.0 24.0 39.0 2.0 41.0 62-63 27.933034577090794 34.0 23.0 39.0 2.0 40.0 64-65 27.65065696388443 34.0 23.0 38.0 2.0 40.0 66-67 27.401763533355137 34.0 22.0 38.0 2.0 40.0 68-69 27.35330146525351 34.0 23.0 37.0 2.0 40.0 70-71 27.130879950768694 34.0 22.0 37.0 2.0 40.0 72-73 26.87150300928047 34.0 22.0 36.0 2.0 39.0 74-75 26.592704670164533 33.5 21.5 36.0 2.0 39.0 76-77 26.319169944690444 33.0 20.0 35.5 2.0 38.5 78-79 26.043356926137065 33.0 20.0 35.0 2.0 37.5 80-81 25.781486893089205 33.0 18.5 35.0 2.0 37.0 82-83 25.55008209421372 33.0 18.0 35.0 2.0 36.5 84-85 25.325421799481624 33.0 17.0 35.0 2.0 36.0 86-87 25.137158707913578 33.0 14.0 35.0 2.0 36.0 88-89 24.9486013718519 33.0 10.5 35.0 2.0 35.5 90-91 24.806296327744647 33.0 8.5 35.0 2.0 35.0 92-93 24.683563166306147 32.0 7.0 35.0 2.0 35.0 94-95 24.559170465923543 32.0 6.0 35.0 2.0 35.0 96-97 24.440172948506373 32.0 2.0 35.0 2.0 35.0 98-99 24.329985951927174 32.0 2.0 35.0 2.0 35.0 100-101 24.221962913424022 32.0 2.0 35.0 2.0 35.0 102-103 23.145511468389316 30.0 2.0 34.0 2.0 35.0 104-105 23.937680697645288 32.0 2.0 35.0 2.0 35.0 106-107 24.065631654654485 32.0 2.0 35.0 2.0 35.0 108-109 24.01691695663761 32.0 2.0 35.0 2.0 35.0 110-111 23.911795168841692 32.0 2.0 35.0 2.0 35.0 112-113 23.79321673965331 32.0 2.0 35.0 2.0 35.0 114-115 23.658071925124023 32.0 2.0 35.0 2.0 35.0 116-117 23.529273964322435 32.0 2.0 35.0 2.0 35.0 118-119 23.368745938375113 32.0 2.0 35.0 2.0 35.0 120-121 23.175544583505328 32.0 2.0 35.0 2.0 35.0 122-123 22.907381921786452 32.0 2.0 35.0 2.0 35.0 124-125 21.545218653083218 29.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4827203877437931 1101 2 0.6282701165710485 1101 3 0.6237523118476247 1101 4 0.545696064499591 1101 5 0.5270690487377507 1101 6 0.541398495589835 1101 7 0.8285736649140105 1101 8 0.46546488746229286 1101 9 0.8381478805182745 1101 10-11 0.9373497577183869 1101 12-13 0.6028086506385719 1101 14-15 0.7480504154562126 1101 16-17 0.646597002096307 1101 18-19 1.029706805671438 1101 20-21 1.481969704627339 1101 22-23 1.3272872739071957 1101 24-25 1.417846157132633 1101 26-27 1.6292813065775746 1101 28-29 1.3698068076449594 1101 30-31 1.5816507890385942 1101 32-33 1.359266315044291 1101 34-35 1.7358674537753664 1101 36-37 1.6145889959513475 1101 38-39 1.4359022459451722 1101 40-41 1.2257873061119895 1101 42-43 1.1932038926369586 1101 44-45 1.1139636994678561 1101 46-47 0.7822064986972812 1101 48-49 0.7859744397642068 1101 50-51 0.7559748990531112 1101 52-53 0.38651512603670923 1101 54-55 0.31780630733085147 1101 56-57 0.4213719769012769 1101 58-59 0.3957726052172994 1101 60-61 0.3314717334393791 1101 62-63 0.132154111966031 1101 64-65 0.18138957778588605 1101 66-67 0.012817537314663952 1101 68-69 -0.011760356861991283 1101 70-71 -0.07677040573258154 1101 72-73 -0.03841488748357591 1101 74-75 0.07991220660601073 1101 76-77 0.10408511103824836 1101 78-79 0.06863564629121655 1101 80-81 0.09045702618773532 1101 82-83 0.06685291301737806 1101 84-85 0.19572694952863756 1101 86-87 0.18844074809974032 1101 88-89 0.18569704419090272 1101 90-91 0.2663568523551234 1101 92-93 0.04362814617464039 1101 94-95 0.1523499104304591 1101 96-97 0.08516493626196464 1101 98-99 0.14413975173378546 1101 100-101 0.2269607085621388 1101 102-103 0.05994350403805271 1101 104-105 0.1403838614638282 1101 106-107 0.17132459207654094 1101 108-109 0.28341791423303064 1101 110-111 0.07150557786824052 1101 112-113 0.05422063930641485 1101 114-115 0.23128963222077914 1101 116-117 0.28398031366035426 1101 118-119 0.25894633910353093 1101 120-121 0.22612870448297784 1101 122-123 0.20800497386655437 1101 124-125 0.3884558122257573 1102 1 -0.4741014524988749 1102 2 -0.18069867345254664 1102 3 -0.1840465422137143 1102 4 -0.004247717097982218 1102 5 0.002056038963566209 1102 6 -0.8054024481243545 1102 7 -1.547167218132838 1102 8 -0.4556149912030243 1102 9 -0.2730904073145517 1102 10-11 -0.489984249696505 1102 12-13 -0.4351483429576568 1102 14-15 -0.12712612650536315 1102 16-17 -0.29635223956193 1102 18-19 -0.13825288425104532 1102 20-21 0.25183084382518217 1102 22-23 0.18777382597998837 1102 24-25 0.12178966909757705 1102 26-27 -0.15197841704898352 1102 28-29 0.31185291482392685 1102 30-31 0.017462181017027234 1102 32-33 -0.19030227275483114 1102 34-35 0.19454167015554802 1102 36-37 0.27405539298538883 1102 38-39 0.3031924380584172 1102 40-41 0.14948666573450353 1102 42-43 -0.09993624892016939 1102 44-45 0.02675978979478444 1102 46-47 -0.18504527076682464 1102 48-49 -0.5147473667743832 1102 50-51 0.004294413714383438 1102 52-53 -0.3000573040273302 1102 54-55 -0.5210900863327836 1102 56-57 -0.4679182152119665 1102 58-59 -0.34451765430410575 1102 60-61 -0.3095291428666549 1102 62-63 -0.4672915233558399 1102 64-65 -0.43605632716861464 1102 66-67 -0.34719580950029894 1102 68-69 -0.5137061607042561 1102 70-71 -0.6806768432115184 1102 72-73 -0.37330211363794064 1102 74-75 -0.795398477586776 1102 76-77 -0.6440699277214428 1102 78-79 -0.7197768916258696 1102 80-81 -1.040305764509938 1102 82-83 -0.8590958567837674 1102 84-85 -1.1972376611892628 1102 86-87 -0.6942605663593113 1102 88-89 -0.5020744421589427 1102 90-91 -1.0753805254675939 1102 92-93 -0.6468527436130245 1102 94-95 -0.7073418994785428 1102 96-97 -0.4844219865556951 1102 98-99 -0.3679438343801351 1102 100-101 -0.4292169793380971 1102 102-103 -0.5428757746946715 1102 104-105 -0.3593173855870653 1102 106-107 -0.39240887607310526 1102 108-109 -0.9121885569499817 1102 110-111 -0.5458725144809371 1102 112-113 -0.9454529704003605 1102 114-115 -0.9578011665658792 1102 116-117 -0.8649402121232654 1102 118-119 -0.44907765820014234 1102 120-121 -0.07087055402730158 1102 122-123 -0.22070510365282558 1102 124-125 0.06603936463559634 1103 1 0.3065319318809081 1103 2 0.24344670684352465 1103 3 0.18732579060915455 1103 4 0.3169398275676656 1103 5 0.1765058603009848 1103 6 0.4876025544279692 1103 7 0.41326483903919 1103 8 0.4966504503487883 1103 9 0.6655966779330242 1103 10-11 0.5469223352958394 1103 12-13 0.5119250618407136 1103 14-15 0.7944448791097471 1103 16-17 0.5098962545677281 1103 18-19 0.6237955077857151 1103 20-21 1.12142961871033 1103 22-23 1.0497201251122021 1103 24-25 1.1431368870659249 1103 26-27 1.1441498116391244 1103 28-29 0.9396928331496035 1103 30-31 0.9901600881124857 1103 32-33 0.9521887941160223 1103 34-35 1.3622533597931188 1103 36-37 1.2631143005913117 1103 38-39 1.384101064908851 1103 40-41 0.9542686973833057 1103 42-43 0.8605811579200555 1103 44-45 0.5187291619234209 1103 46-47 0.5747158318365457 1103 48-49 0.5237432917248519 1103 50-51 0.5196961492937646 1103 52-53 0.5478757230272926 1103 54-55 0.4938314042236236 1103 56-57 0.4495641737619991 1103 58-59 0.36008796080564665 1103 60-61 0.1124816789812293 1103 62-63 0.15846161600097375 1103 64-65 0.3535424477165314 1103 66-67 0.5991233329193015 1103 68-69 0.4892986376622588 1103 70-71 0.3897982971662266 1103 72-73 0.35548849381483905 1103 74-75 0.38160693333716367 1103 76-77 0.32134712179361813 1103 78-79 0.2842761966844911 1103 80-81 0.2809226173175219 1103 82-83 0.41480919681500694 1103 84-85 0.4360201233947123 1103 86-87 0.3574033437989357 1103 88-89 0.3127984521748992 1103 90-91 0.2279015572276606 1103 92-93 0.23930019155798377 1103 94-95 0.20084145937801878 1103 96-97 0.3030761247255711 1103 98-99 0.20714753876943348 1103 100-101 0.2891589267364303 1103 102-103 0.18575597619864226 1103 104-105 0.3464239109928222 1103 106-107 0.38213650422296297 1103 108-109 0.6130839290122445 1103 110-111 0.5470226361060959 1103 112-113 0.5481576472617107 1103 114-115 0.6697867864713842 1103 116-117 0.598221809316243 1103 118-119 0.523385026843247 1103 120-121 0.5948025444923495 1103 122-123 0.4660358866483456 1103 124-125 0.41634013033238304 1104 1 0.22275797145261578 1104 2 0.34040194975782967 1104 3 -0.009848933994074827 1104 4 0.1384498069040383 1104 5 0.027740660826459873 1104 6 0.01735355659183213 1104 7 0.06951379921598999 1104 8 0.21448505932754713 1104 9 0.5521721832193869 1104 10-11 0.25086129519148415 1104 12-13 0.20936275692444184 1104 14-15 0.2316841817311186 1104 16-17 0.2989811611454982 1104 18-19 0.6443495075293129 1104 20-21 0.6945783594054404 1104 22-23 0.4809202635747347 1104 24-25 1.0081271459377312 1104 26-27 1.0745187363955182 1104 28-29 0.9116964721761214 1104 30-31 0.8069035823981956 1104 32-33 0.8509677289295219 1104 34-35 1.1457610738948105 1104 36-37 1.1918885073529175 1104 38-39 1.1985941560978333 1104 40-41 0.7876581328054151 1104 42-43 0.9247032659863486 1104 44-45 0.7528712939214444 1104 46-47 0.619922894973449 1104 48-49 0.5214658048611227 1104 50-51 0.33518476237532724 1104 52-53 0.48491396092130046 1104 54-55 0.4556661205056862 1104 56-57 0.421312709497478 1104 58-59 0.26875221263205873 1104 60-61 0.38853142644444816 1104 62-63 0.42113481991517077 1104 64-65 0.45583048435235796 1104 66-67 0.46003774279883913 1104 68-69 0.5623314340405656 1104 70-71 0.5325881727898292 1104 72-73 0.3974869043846283 1104 74-75 0.5517608284600506 1104 76-77 0.5235123528916645 1104 78-79 0.5885303342457853 1104 80-81 0.5457874138629748 1104 82-83 0.5602135587459642 1104 84-85 0.6444055929795098 1104 86-87 0.6862334205027416 1104 88-89 0.6747401106455015 1104 90-91 0.7364862085731687 1104 92-93 0.7956341689690909 1104 94-95 0.7626263673228735 1104 96-97 0.5929602603703614 1104 98-99 0.7714652948096941 1104 100-101 0.7087737904109339 1104 102-103 0.6836246127063745 1104 104-105 0.7871184574312942 1104 106-107 0.6287464549021351 1104 108-109 0.7408042677089348 1104 110-111 0.7506221461220619 1104 112-113 0.68111559958448 1104 114-115 0.6944417844194675 1104 116-117 0.693607741388611 1104 118-119 0.6850909791587938 1104 120-121 0.6933175544449917 1104 122-123 0.7579509179068857 1104 124-125 0.947333890490409 1105 1 0.11341725652784973 1105 2 0.3098356684536583 1105 3 0.4127163423046909 1105 4 0.4268348850200212 1105 5 0.5392453480911499 1105 6 0.39544555994422836 1105 7 0.35358759780261195 1105 8 0.2529356895836621 1105 9 0.1474118619744047 1105 10-11 0.4266067740049593 1105 12-13 0.4380849399470321 1105 14-15 0.46946045947547077 1105 16-17 0.46799955065491616 1105 18-19 0.5513354163036723 1105 20-21 0.8098883324787352 1105 22-23 1.1206299026315278 1105 24-25 1.241764656490414 1105 26-27 1.0007509828501604 1105 28-29 0.7502900303055178 1105 30-31 0.8087694931703275 1105 32-33 0.9406508286067385 1105 34-35 1.4619881653648292 1105 36-37 1.4985419285375805 1105 38-39 1.4370804864699167 1105 40-41 1.0428381533794884 1105 42-43 0.600761280076302 1105 44-45 1.0297431483424404 1105 46-47 1.0087338517747817 1105 48-49 0.7469865717026671 1105 50-51 0.8821885135631007 1105 52-53 0.7877538911315227 1105 54-55 0.6612873216349442 1105 56-57 0.6800273218530855 1105 58-59 0.5768786139762767 1105 60-61 0.5541674784142323 1105 62-63 0.6273452895538938 1105 64-65 0.6466405966841329 1105 66-67 0.45368689347499114 1105 68-69 0.36349847873094276 1105 70-71 0.560699657040626 1105 72-73 0.7052806397659914 1105 74-75 0.7485668275065187 1105 76-77 0.8785342074273217 1105 78-79 0.9422402541129102 1105 80-81 0.8733587841537016 1105 82-83 0.7321598164386245 1105 84-85 0.7341410579231962 1105 86-87 0.6345620256729632 1105 88-89 0.7417137575384842 1105 90-91 0.7650608260200507 1105 92-93 0.5361140743395758 1105 94-95 0.6698413582118263 1105 96-97 0.8018239035098595 1105 98-99 0.8110011983984862 1105 100-101 0.6808388955988676 1105 102-103 0.6160829746749705 1105 104-105 0.7064145357442548 1105 106-107 0.6202349968467118 1105 108-109 0.603810232762573 1105 110-111 0.5858063559174518 1105 112-113 0.6843898939465873 1105 114-115 0.6926565238022526 1105 116-117 0.6507293794047229 1105 118-119 0.7038284436657172 1105 120-121 0.6029989366433988 1105 122-123 0.775183549902394 1105 124-125 1.0002049723007538 1106 1 -0.19272745045179107 1106 2 -0.05208482152487548 1106 3 -0.04895763057946212 1106 4 -0.14080943664762202 1106 5 -0.11463969628037773 1106 6 -0.1003423321229917 1106 7 -0.48805510622564086 1106 8 -0.06138367083455165 1106 9 -0.11236301052164777 1106 10-11 0.16608876292717412 1106 12-13 0.08239215516585219 1106 14-15 0.40763620716915483 1106 16-17 0.3855232977973273 1106 18-19 0.3377570782191057 1106 20-21 0.5387452075235792 1106 22-23 0.5338110828888958 1106 24-25 0.7571573934237463 1106 26-27 0.8974507197643895 1106 28-29 0.6548162720398913 1106 30-31 0.5235102192292409 1106 32-33 0.34706040210016553 1106 34-35 0.7531103497870717 1106 36-37 0.6512227761310747 1106 38-39 0.7753009334524776 1106 40-41 0.3485525135680092 1106 42-43 0.31383120246161056 1106 44-45 0.19863355301552588 1106 46-47 -0.022899927880438042 1106 48-49 -0.025897554423650604 1106 50-51 -0.1246042556817244 1106 52-53 -0.13756422999116324 1106 54-55 -0.2266101136272205 1106 56-57 -0.21664492964390192 1106 58-59 -0.28132024727578653 1106 60-61 -0.15527707872471908 1106 62-63 -0.08761441656634616 1106 64-65 -0.1429938911399553 1106 66-67 -0.20835486784216073 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-0.332362885329907 2216 90-91 -0.2535688731275769 2216 92-93 -0.34867814953470244 2216 94-95 -0.46393253940212986 2216 96-97 -0.25722962743439837 2216 98-99 -0.3571205297000972 2216 100-101 -0.32416712260457103 2216 102-103 -0.0853621166239904 2216 104-105 -0.3390904514896427 2216 106-107 -0.29138194197568623 2216 108-109 -0.27725980814387086 2216 110-111 -0.3288211295716721 2216 112-113 -0.2690770391682662 2216 114-115 -0.3190634397272909 2216 116-117 -0.3694529150841035 2216 118-119 -0.24847097243127791 2216 120-121 -0.12977332384583207 2216 122-123 -0.15623060508549003 2216 124-125 -0.06508422657739033 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 105144.0 3 17581.0 4 12500.0 5 11507.0 6 10481.0 7 9113.0 8 9302.0 9 9656.0 10 10057.0 11 10601.0 12 11068.0 13 12048.0 14 13141.0 15 11420.0 16 8728.0 17 7728.0 18 7171.0 19 6800.0 20 6798.0 21 7168.0 22 8391.0 23 12129.0 24 17578.0 25 18478.0 26 23816.0 27 24584.0 28 27485.0 29 30827.0 30 34515.0 31 39892.0 32 48180.0 33 59611.0 34 76807.0 35 105084.0 36 148215.0 37 166790.0 38 48998.0 39 95.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.55809338832204 19.521581387918456 13.114723774209958 22.80560144954954 2 14.830564761707787 21.78192432963238 39.26638316762259 24.12112774103724 3 19.041497390228155 27.769099090615583 27.844012322427897 25.345391196728368 4 11.911550183920125 17.878970047293745 36.05910667367315 34.15037309511298 5 13.06034247180052 40.365006159111076 32.16914222291358 14.405509146174836 6 30.59090974054853 38.032116798139675 17.07397658286751 14.302996878444283 7 27.38799683197885 35.18908075258999 19.991071023200206 17.43185139223095 8 25.60044792418917 36.842857467126585 18.941274683960398 18.615419924723852 9 26.42966256087852 18.592318020209188 18.77441761338558 36.203601805526716 10-11 24.25591872798946 29.11456787674014 25.830799327777438 20.79871406749296 12-13 25.112699904661177 26.74501993371682 26.289680371132008 21.852599790489993 14-15 22.287748194158496 27.850425325547572 24.545191969328968 25.316634510964963 16-17 21.65978701315957 31.025380224020076 25.037201426751977 22.277631336068378 18-19 21.678379002123314 29.268829942101814 27.58527192558025 21.46751913019462 20-21 22.634528704978084 28.2840911288363 27.569188653371928 21.51219151281368 22-23 22.20089132958765 28.311946859874954 27.59019016208209 21.896971648455306 24-25 21.889050367025277 28.453003486330246 27.658500226382348 21.999445920262126 26-27 21.75902763471556 28.66678158358314 27.557525025961414 22.01666575573989 28-29 21.63803235357912 28.64124887976447 27.709835037436463 22.010883729219945 30-31 21.860366381665784 28.56923671887479 27.35617543948145 22.214221459977974 32-33 21.66983987923155 28.57858714199415 27.241591967603384 22.50998101117091 34-35 22.023135651326555 28.480132237352933 27.61955528800174 21.877176823318774 36-37 22.308375614778306 28.198392148998845 27.30210539104714 22.191126845175713 38-39 21.950933469638592 28.472694531339982 27.187644757782138 22.38872724123929 40-41 21.9975487877431 28.627426248152656 27.34829708019837 22.026727883905878 42-43 21.93178932783128 28.54543650693558 27.015682199207312 22.507091966025826 44-45 22.041249960060068 28.722317424067885 26.94452806137464 22.291904554497403 46-47 22.320476361626238 28.973270866568722 26.318517444243366 22.38773532756167 48-49 22.154637246389914 29.056363403205086 26.180402412541177 22.60859693786382 50-51 22.21117128872194 29.26056924171166 26.09278132098917 22.435478148577232 52-53 22.422102973802993 28.917802380353884 26.06724579587671 22.592848849966416 54-55 22.168983577720994 29.113116213836893 26.08608096029678 22.631819248145334 56-57 22.18075044348974 29.339053159916933 25.743633505124304 22.736562891469024 58-59 22.24095584343961 28.786948284696678 25.972281246440154 22.999814625423557 60-61 22.150395251517004 29.034266341301418 26.15125280333268 22.664085603848893 62-63 22.105966424148242 28.969599424144874 26.256571711117164 22.667862440589715 64-65 22.013013575672677 29.034139089789633 26.096436002292112 22.856411332245575 66-67 22.064340718028998 29.05727745552726 26.073707958497288 22.80467386794646 68-69 22.390111045149617 28.869846945039125 26.12382131199096 22.6162206978203 70-71 22.19399261424899 28.898547861305936 26.051691849821662 22.855767674623408 72-73 22.169915428631004 29.00293059110834 25.906408581826806 22.92074539843385 74-75 22.145975533990704 29.30960994109225 25.971532265590124 22.57288225932692 76-77 22.334957949704435 28.87833369345616 26.077615017206025 22.709093339633384 78-79 22.32505937493432 28.948023287584856 26.065028689127555 22.661888648353266 80-81 22.362203532825617 29.094716062834376 25.963170202560942 22.579910201779064 82-83 22.24652344869854 29.145694423309898 25.94968847443691 22.658093653554655 84-85 22.30263970938732 29.178587071569506 25.879938158214422 22.638835060828743 86-87 22.347516874842626 29.00358452470682 25.945325383790486 22.703573216660075 88-89 22.447907951725778 29.091331038965297 25.64830751818944 22.812453491119484 90-91 22.271932258688395 29.107111324134106 25.878972108002657 22.741984309174846 92-93 22.270167790806923 28.93892229665112 26.0732579329131 22.717651979628855 94-95 22.050871522853587 28.793027985349955 26.27219800683058 22.88390248496588 96-97 22.416836097195105 28.62089435775319 25.96806633940929 22.994203205642417 98-99 22.185030292763493 28.761374235955262 25.80708892280673 23.246506548474517 100-101 22.05844951027232 28.76090216334401 26.075860181581163 23.104788144802505 102-103 22.0828811075699 28.548021121710455 26.20116907540814 23.167928695311506 104-105 21.853049701675612 28.72245533120859 26.10554890931056 23.31894605780524 106-107 21.866284316803934 28.884480824288467 26.03056024595487 23.218674612952732 108-109 21.903770280801723 29.00670625235921 25.79359841679649 23.29592505004258 110-111 21.88425955700829 28.992152456471544 25.896347186860147 23.22724079966002 112-113 21.977247334355063 29.15609838527029 25.96720266804093 22.89945161233372 114-115 21.790107836403426 29.412511444009056 25.756061226394234 23.041319493193285 116-117 21.641724541756236 29.4531592190583 25.89011061070865 23.015005628476814 118-119 21.591114488851076 29.480734131604635 25.751647558989717 23.176503820554576 120-121 21.62151483013889 29.51950274341071 25.81803156235905 23.040950864091354 122-123 21.55883166440659 29.469426736063532 25.75673378523683 23.215007814293052 124-125 21.674122346016397 29.493420222829513 25.59335715787261 23.23910027328148 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7207.0 1 7154.0 2 7396.5 3 5826.0 4 2997.5 5 1686.5 6 1237.5 7 1361.5 8 1848.0 9 1966.0 10 1488.0 11 1089.0 12 1034.5 13 1080.5 14 1140.0 15 1215.5 16 1291.0 17 1377.0 18 1503.0 19 1643.5 20 1888.0 21 2210.0 22 2650.0 23 3313.0 24 4014.5 25 4998.5 26 6572.0 27 8332.5 28 9964.5 29 11700.0 30 13677.0 31 16567.5 32 19860.5 33 21739.5 34 22939.5 35 24213.0 36 26039.5 37 28124.5 38 29239.0 39 29928.5 40 31091.5 41 32442.5 42 32846.0 43 33821.5 44 35519.5 45 37133.0 46 38072.5 47 38279.0 48 39737.5 49 40378.5 50 40130.5 51 39573.5 52 38693.0 53 38942.0 54 38934.5 55 37053.5 56 34718.5 57 33191.5 58 32305.5 59 29953.5 60 25540.5 61 21468.0 62 18052.5 63 14035.5 64 10487.0 65 8611.5 66 6926.5 67 5407.0 68 4313.0 69 3395.0 70 2610.5 71 2077.5 72 1704.0 73 1469.0 74 1210.0 75 922.0 76 681.0 77 478.5 78 345.5 79 251.5 80 186.0 81 137.5 82 106.5 83 86.0 84 64.0 85 42.0 86 28.5 87 20.0 88 22.5 89 22.0 90 17.5 91 12.5 92 3.0 93 5.0 94 8.5 95 5.5 96 1.5 97 1.0 98 1.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012778618009276268 2 0.005464540596072088 3 0.009331753940984644 4 0.009415823796308828 5 0.016141412222243707 6 0.0013451176851869756 7 0.008491055387742783 8 0.0 9 0.002353955949077207 10-11 0.0 12-13 0.0037831434895883686 14-15 0.018621472954307192 16-17 0.029634624001775557 18-19 0.006010994655679297 20-21 0.007902566400473482 22-23 0.002353955949077207 24-25 0.010718906553833712 26-27 0.01866350788196929 28-29 4.203492766209298E-4 30-31 0.0014291875405111616 32-33 0.008701230026053249 34-35 0.00928971901332255 36-37 0.0113914653964272 38-39 0.0 40-41 0.02349752456310998 42-43 0.009499893651633014 44-45 0.01689804092016138 46-47 0.004581807115168136 48-49 0.02391787383973091 50-51 0.0037831434895883686 52-53 0.0 54-55 0.015805132800946965 56-57 0.0047919817534786005 58-59 2.942444936346509E-4 60-61 0.004581807115168136 62-63 0.025136886741931604 64-65 0.01601530743925743 66-67 0.0018915717447941843 68-69 0.02026083513312882 70-71 0.002900410008684416 72-73 0.011475535251751386 74-75 0.0 76-77 0.006557448715286507 78-79 0.0010088382638902317 80-81 0.0035729688512779042 82-83 0.0015132573958353476 84-85 0.0 86-87 0.005758785089706739 88-89 0.016645831354188824 90-91 0.021605952818315795 92-93 0.005002156391789065 94-95 0.023161245141813235 96-97 0.017570599762754865 98-99 9.668033362281387E-4 100-101 0.008533090315404876 102-103 0.0 104-105 8.406985532418596E-4 106-107 4.203492766209299E-5 108-109 0.0 110-111 5.464540596072087E-4 112-113 0.0 114-115 0.0 116-117 0.0 118-119 0.0 120-121 4.203492766209299E-5 122-123 0.0 124-125 0.019924555711832076 >>END_MODULE >>Sequence Length Distribution pass #Length Count 125 1189487.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.078307909221 #Duplication Level Percentage of deduplicated Percentage of total 1 77.26652843168067 41.78443115605006 2 10.631076337164522 11.498212391352329 3 4.516296959980803 7.327010928339618 4 2.3561001098301815 5.096556288173838 5 1.3597585891139254 3.6766721832155396 6 0.8954852206483047 2.905579529422544 7 0.6375718357229269 2.4135164232530197 8 0.44893781797849636 1.9422238042187951 9 0.31753516793311093 1.5454588125143676 >10 1.4664487065861171 13.481838474134628 >50 0.06830915203767306 2.502425166993092 >100 0.0327701107246548 3.169312054896899 >500 0.002068014389129144 0.7488624830691548 >1k 9.544681795980664E-4 1.2219233439993422 >5k 1.5907802993301104E-4 0.6859769603668513 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 7974 0.6703730263550589 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3480 0.29256309652816714 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2918 0.24531583783597466 No Hit GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2513 0.21126754642967932 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2431 0.2043738182930961 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1624 0.13652944504647802 No Hit AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1238 0.10407848089134222 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.203492766209299E-5 0.0 12-13 0.0 0.0 0.0 8.406985532418597E-5 0.0 14-15 0.0 0.0 0.0 8.406985532418597E-5 0.0 16-17 0.0 0.0 0.0 2.522095659725579E-4 0.0 18-19 4.203492766209299E-5 0.0 0.0 4.2034927662092987E-4 0.0 20-21 8.406985532418597E-5 0.0 0.0 5.884889872693018E-4 0.0 22-23 8.406985532418597E-5 0.0 0.0 0.0017654669618079053 0.0 24-25 8.406985532418597E-5 0.0 0.0 0.00323668942998116 0.0 26-27 8.406985532418597E-5 0.0 0.0 0.004161457838547206 0.0 28-29 1.2610478298627896E-4 0.0 0.0 0.005422505668409995 0.0 30-31 1.6813971064837195E-4 0.0 0.0 0.010382627132536968 0.0 32-33 2.1017463831046493E-4 8.406985532418597E-5 0.0 0.015594958162636497 0.0 34-35 2.522095659725579E-4 8.406985532418597E-5 0.0 0.023035140358826957 0.0 36-37 2.522095659725579E-4 8.406985532418597E-5 0.0 0.03480492010421299 0.0 38-39 3.362794212967439E-4 8.406985532418597E-5 0.0 0.056494942777852976 0.0 40-41 3.783143489588369E-4 8.406985532418597E-5 0.0 0.08167386444744668 0.0 42-43 4.2034927662092987E-4 8.406985532418597E-5 0.0 0.10618022727444688 0.0 44-45 4.2034927662092987E-4 8.406985532418597E-5 0.0 0.13396531445909035 0.0 46-47 4.2034927662092987E-4 8.406985532418597E-5 0.0 0.16822378050369613 0.0 48-49 4.2034927662092987E-4 8.406985532418597E-5 0.0 0.21063702251474795 0.0 50-51 4.2034927662092987E-4 8.406985532418597E-5 0.0 0.25805242091758884 0.0 52-53 4.2034927662092987E-4 8.406985532418597E-5 0.0 0.30588816859705065 0.0 54-55 4.2034927662092987E-4 8.406985532418597E-5 0.0 0.35452257990209224 0.0 56-57 4.2034927662092987E-4 8.406985532418597E-5 0.0 0.4125307800757806 0.0 58-59 4.2034927662092987E-4 8.406985532418597E-5 0.0 0.47789509259033514 0.0 60-61 4.2034927662092987E-4 8.406985532418597E-5 0.0 0.5548610451396274 0.0 62-63 4.2034927662092987E-4 8.406985532418597E-5 0.0 0.630187635510098 0.0 64-65 4.2034927662092987E-4 8.406985532418597E-5 0.0 0.7050938766039478 0.0 66-67 4.2034927662092987E-4 8.406985532418597E-5 0.0 0.79832734615847 0.0 68-69 4.2034927662092987E-4 8.406985532418597E-5 0.0 0.9058106561904418 0.0 70-71 4.623842042830228E-4 8.406985532418597E-5 0.0 1.0285106100360912 0.0 72-73 5.044191319451158E-4 8.406985532418597E-5 0.0 1.158608711150269 0.0 74-75 5.044191319451158E-4 8.406985532418597E-5 0.0 1.2871515199409491 0.0 76-77 5.044191319451158E-4 8.406985532418597E-5 0.0 1.4296919596431068 0.0 78-79 5.044191319451158E-4 8.406985532418597E-5 0.0 1.5949312602827943 0.0 80-81 5.884889872693018E-4 8.406985532418597E-5 0.0 1.7840043649068884 0.0 82-83 5.884889872693018E-4 8.406985532418597E-5 0.0 1.9814844550634012 0.0 84-85 5.884889872693018E-4 8.406985532418597E-5 0.0 2.180772047109384 0.0 86-87 5.884889872693018E-4 8.406985532418597E-5 0.0 2.384683481198197 0.0 88-89 5.884889872693018E-4 8.406985532418597E-5 0.0 2.624282568872127 0.0 90-91 5.884889872693018E-4 1.2610478298627896E-4 0.0 2.900452043612078 0.0 92-93 5.884889872693018E-4 1.6813971064837195E-4 0.0 3.201380090745002 0.0 94-95 5.884889872693018E-4 1.6813971064837195E-4 0.0 3.5085713420995774 0.0 96-97 5.884889872693018E-4 1.6813971064837195E-4 0.0 3.819335562305431 0.0 98-99 6.305239149313948E-4 1.6813971064837195E-4 0.0 4.142247876605629 0.0 100-101 7.145937702555808E-4 1.6813971064837195E-4 0.0 4.524303334126392 0.0 102-103 7.566286979176737E-4 1.6813971064837195E-4 0.0 4.948477789164572 0.0 104-105 8.406985532418597E-4 1.6813971064837195E-4 0.0 5.367271773462005 0.0 106-107 8.406985532418597E-4 1.6813971064837195E-4 0.0 5.784720640074251 0.0 108-109 8.406985532418597E-4 1.6813971064837195E-4 0.0 6.228273196764656 0.0 110-111 9.247684085660457E-4 1.6813971064837195E-4 0.0 6.728783080437196 0.0 112-113 9.247684085660457E-4 1.6813971064837195E-4 0.0 7.262332417252143 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5255 0.0 43.36517 1 GGTATCA 3385 0.0 39.900852 1 GTACATG 6755 0.0 35.232998 1 TATCAAC 6595 0.0 34.91493 2 ATCAACG 6505 0.0 34.39185 3 AACGCAG 6570 0.0 34.13785 6 TCAACGC 6535 0.0 34.051872 4 CAACGCA 6590 0.0 33.948254 5 TACATGG 6875 0.0 33.666027 2 ACATGGG 7300 0.0 30.401915 3 ACGCAGA 7485 0.0 29.646759 7 CGCAGAG 7480 0.0 28.871227 8 CATGGGG 2895 0.0 27.745987 4 GAGTACT 3150 0.0 27.301205 12-13 ATACCGA 90 0.009535166 26.440987 6 GAACAAA 1680 0.0 25.854048 1 GCAGAGT 7830 0.0 25.833149 9 CATGGGA 4605 0.0 25.712152 4 AGTACTT 3390 0.0 24.75392 12-13 GTACTTT 3570 0.0 23.755884 14-15 >>END_MODULE