FastQCFastQC Report
Thu 26 May 2016
SRR1364527_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1364527_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1189487
Sequences flagged as poor quality0
Sequence length125
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99760.8386808767140793No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83460.7016470125356561No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71640.6022764435424683No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24860.20899766033592634No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22550.18957752375603937No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19280.16208668106503055No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18120.152334577847425No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18000.15132573958353474No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48500.050.874291
GTATCAA78800.045.2287641
TATCAAC104850.033.8452032
ATCAACG107600.033.5888983
TCAACGC107750.033.376364
CAACGCA110500.032.467265
AACGCAG111400.032.418596
ACGCAGA124250.028.7785347
CGCAGAG124400.028.6960078
GTACATG62700.028.5163171
TACATGG64150.027.4735222
GCGTATC1107.6763873E-427.0905021
GCAGAGT129200.026.8010049
GAGTACT84350.026.59174712-13
AGTACTT86750.026.50761212-13
TAAGGTG5950.025.9984235
CATGGGG27000.024.9191954
GTACTTT89500.024.76246314-15
ACATGGG67300.024.5951253
AGAGTAC123200.024.12211410-11
Produced by FastQC (version 0.11.5)